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| 1 | Identification of the novel recessive gene pi55(t) conferring resistance to Magnaporthe oryzae显示文摘The elite rice cultivar Yuejingsimiao 2 (YJ2) is characterized by a high level of grain quality and yield, and resistance against Magnaporthe oryzae. YJ2 showed 100% resistance to four fungal populations collected from Guangdong, Sichuan, Liaoning, and Heilongjiang Provinces, which is a higher frequency than that shown by the well-known resistance (R) gene donor cultivars such as Sanhuangzhan 2 and 28zhan. Segregation analysis for resistance with F 2 and F 4 populations indicated the resistance of YJ2 was controlled by multiple genes that are dominant or recessive. The putative R genes of YJ2 were roughly tagged by SSR markers, located on chromosomes 2, 6, 8, and 12, in a bulked-segregant analysis using genome-wide selected SSR markers with F 4 lines that segregated into 3 resistant (R):1 susceptible (S) or 1R:3S. The recessive R gene on chromosome 8 was further mapped to an interval 1.9 cM/152 kb in length by linkage analysis with genomic position-ready markers in the mapping population derived from an F 4 line that segregated into 1R:3S. Given that no major R gene was mapped to this interval, the novel R gene was designated as pi55(t). Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare, two genes that encode a leucine-rich repeat-containing protein and heavy-metalassociated domain-containing protein, respectively, were suggested as the most likely candidates for pi55(t). | HE XiuYing LIU XinQiong WANG Li WANGLing LIN Fei CHENG YongSheng CHEN ZhaoMing LIAO YaoPing PAN QingHua | 2012 | Science China(Life Sciences)2012，55，2： | 15 |
| 2 | Key vacuum technology issues to be solved in evacuated tube transportation显示文摘Evacuated tube transportation(ETT) will be one of the ultra-large-scale vacuum application areas.This paper lists some key vacuum technology issues in ETT:(1) how to construct ultra-large-scale vacuum chamber with lower cost and high reliability,(2) how to evacuate gas out of the ETT tube in short time,(3) how to release heat or reduce temperature in the vacuum tube,(4) how to avoid vacuum discharge,(5) how to make vehicles with airproof shells and life support system,and(6) how to detect leaks and find leak positions efficiently.At the same time,some solutions are proposed. | Yaoping ZHANG Daryl OSTER Masayuki KUMADA Jianye YU Shengshan LI | 2011 | Journal of Modern Transportation2011，19，2： | 13 |
| 3 | 2.5-Dimensional Parylene C micropore array with a large area and a high porosity for high-throughput particle and cell separation显示文摘Large-area micropore arrays with a high porosity are in high demand because of their promising potential in liquid biopsy with a large volume of clinical sample.However,a micropore array with a large area and a high porosity faces a serious mechanical strength challenge.The filtration membrane may undergo large deformation at a high filtration throughput,which will decrease its size separation accuracy.In this work,a keyhole-free Parylene molding process has been developed to prepare a large(>20 mm×20 mm)filtration membrane containing a 2.5-dimensional(2.5D)micropore array with an ultra-high porosity(up to 91.37% with designed pore diameter/space of 100μm/4μm).The notation 2.5D indicates that the large area and the relatively small thickness(approximately 10μm)of the fabricated membranes represent 2D properties,while the large thickness-to-width ratio(10μm/<4μm)of the spaces between the adjacent pores corresponds to a local 3D feature.The large area and high porosity of the micropore array achieved filtration with a throughput up to 180 mL/min(PBS solution)simply driven by gravity.Meanwhile,the high mechanical strength,benefiting from the 2.5D structure of the micropore array,ensured a negligible pore size variation during the high-throughput filtration,thereby enabling high size resolution separation,which was proven by single-layer and multi-layer filtrations for particle separation.Furthermore,as a preliminary demonstration,the prepared 2.5-dimensional Parylene C micropore array was implemented as an efficient filter for rare cancer cell separation from a large volume,approximately 10 cells in 10 mL PBS and undiluted urine,with high recovery rates of 87±13% and 56±13%,respectively. | Yaoping Liu Han Xu Wangzhi Dai Haichao Li Wei Wang | 2018 | Microsystems & Nanoengineering2018，4，1： | 2 |
| 4 | The relationships between urban-rural temperature difference and vegetation in eight cities of the Great Plains显示文摘Interpreting the relationship between urban heat island (UHI) and urban vegetation is a basis for understanding the impacts of underlying surfaces on UHL The calculation of UHI intensity (UHII) requires observations from paired stations in both urban and rural areas.Due to the limited number of paired meteorological stations,many studies have used remotely sensed land surface temperature,but these time-series land surface temperature data are often heavily affected by cloud cover and other factors.These factors,together with the algorithm for inversion of land surface temperature,lead to accuracy problems in detecting the UHII,especially in cities with weak UHII.Based on meteorological observations from the Oklahoma Mesonet,a world-class network,we quantified the UHII and trends in eight cities of the Great Plains,USA,where data from at least one pair of urban and rural meteorological stations were available.We examined the changes and variability in urban temperature,UHII,vegetation condition (as measured by enhanced vegetation index,EVI),and evapotranspiration (ET).We found that both UHI and urban cold islands (UCI) occurred among the eight cities during 2000-2014 (as measured by impervious surface area).Unlike what is generally considered,UHII in only three cities significantly decreased as EVI and ET increased (p < 0.1),indicating that the UHI or UCI cannot be completely explained simply from the perspective of the underlying surface.Increased vegetative cover (signaled by EVI) can increase ET,and thereby effectively mitigate the UHI.Each study station clearly showed that the underlying surface or vegetation affects urban-rural temperature,and that these factors should be considered during analysis of the UHI effect over time. | Yaoping CUI Xiangming XIAO Russell B DOUGHTY Yaochen QIN Sujie LIU Nan LI Guosong ZHAO Jinwei DONG | 2019 | Frontiers of Earth Science2019，13，2： | 2 |
| 5 | Square nearly nonpositive sign pattern matrices 显示文摘 | | 2001 | Linear Algebra and Its Applications2001，327，1： | 1 |
| 6 | Hydrogen production by draTGB hupL double mutant of Rhodospirillum rubrum under different light conditions显示文摘To increase H2 yield of Rhodospirillum rubrum in two-stage hydrogen production process, two deletion mutants were constructed. One is single mutant designated R. rubrum UR801 that deleted hupL gene encoding the large subunit of uptake hy- drogenase, and the other is a double mutant desig- nated R. rubrum UR805 lacked both draTGB encod- ing regulators for the activity of nitrogenase and hupL. Comparing H2 yields of two mutants with R. rubrum UR2 (wild type) and UR472 (ΔdraTGB) under differ- ent light conditions, the results showed that the H2 yield of R. rubrum UR801 under continuous light is the highest (5700 mL of H2 per liter culture), and it is 1.56, 2.24 and 2.32-fold that of R. rubrum UR2, UR472 and UR805, respectively. However, the total H2 yield of R. rubrum UR805 in two-stage hydrogen production process is the highest (4303 mL/L), and it is 1.35, 1.21 and 1.04-fold that of R. rubrum UR2, UR801 and UR472, respectively. Thus, R. rubrum UR805 might be a valuable strain to produce a large amount of hydrogen in two-stage hydrogen produc- tion process. | ZHU Ruiyan WANG Di ZHANG Yaoping LI Jilun | 2006 | Chinese Science Bulletin2006，51，21： | 1 |
| 7 | A New Robust Multiple Description Coding Method Based on Region of Interest 显示文摘 | Song Xiao Fang Zhang Chengke Wu Yunsong Li Yaoping Yan | 2003 | IEEE AINA2003，，： | 1 |
| 8 | Hypertensive epidemiobgy in Heilongjiang province in China显示文摘 | FU Shiying CAO Yamin LI Yaoping | 2002 | Chin Med J(Engl)2002，115，4： | 1 |
| 9 | Nanostructure Forma- tion and Passivation of Large-Area Black Silicon for Solar Cell Applications显示文摘 | Liu Yaoping I_ai Tao Li Hailing | 2012 | Small2012，8，9： | 1 |
| 10 | Enhanced YOLOv5 network-based object detection(BALFilter Reader)promotes PERFECT filter-enabled liquid biopsy of lung cancer from bronchoalveolar lavage fluid(BALF)显示文摘Liquid biopsy of cancers,detecting tumor-related information from liquid samples,has attracted wide attentions as an emerging technology.Our previously reported large-area PERFECT(Precise-Efficient-Robust-Flexible-Easy-ControllableThin)filter has demonstrated competitive sensitivity in recovering rare tumor cells from clinical samples.However,it is time-consuming and easily biased to manually inspect rare target cells among numerous background cells distributed in a large area(Φ≥13 mm).This puts forward an urgent demand for rapid and bias-free inspection.Hereby,this paper implemented deep learning-based object detection for the inspection of rare tumor cells from large-field images of PERFECT filters with hematoxylin-eosin(HE)-stained cells recovered from bronchoalveolar lavage fluid(BALF).CenterNet,EfficientDet,and YOLOv5 were trained and validated with 240 and 60 image blocks containing tumor and/or background cells,respectively.YOLOv5 was selected as the basic network given the highest mAP@0.5 of 92.1%,compared to those of CenterNet and EfficientDet at 85.2%and 91.6%,respectively.Then,tricks including CIoU loss,image flip,mosaic,HSV augmentation and TTA were applied to enhance the performance of the YOLOv5 network,improving mAP@0.5 to 96.2%.This enhanced YOLOv5 network-based object detection,named as BALFilter Reader,was tested and cross-validated on 24 clinical cases.The overall diagnosis performance(~2 min)with sensitivity@66.7%±16.7%,specificity@100.0%±0.0%and accuracy@75.0%±12.5%was superior to that from two experienced pathologists(10–30 min)with sensitivity@61.1%,specificity@16.7%and accuracy@50.0%,with the histopathological result as the gold standard.The AUC of the BALFilter Reader is 0.84±0.08.Moreover,a customized Web was developed for a user-friendly interface and the promotion of wide applications.The current results revealed that the developed BALFilter Reader is a rapid,bias-free and easily accessible AI-enabled tool to promote the transplantation of the BALFilter technique.This work can easily expand to other cytopathological diagnoses and improve the application value of micro/nanotechnology-based liquid biopsy in the era of intelligent pathology. | Zheng Liu Jixin Zhang Ningyu Wang Yun’ai Feng Fei Tang Tingyu Li Liping Lv Haichao Li Wei Wang Yaoping Liu | 2023 | Microsystems & Nanoengineering2023，9，5： | 0 |
| 11 | Transcriptome Analysis Reveals New Insights into the Respiration Metabolism Mechanism of Different Feeding Rations of Sea Cucumber(Apostichopus japonicus)显示文摘Sea cucumber(Apostichopus japonicus)is an excellent model for investigating effects of bottom-dwellers on carbon mig-ration and transformation.However,the molecular mechanism of respiratory metabolism process variation caused by feeding rations is poorly understood.In this study,treatment groups set as 1%(about 0.63g),3%,and 7%of total body weight(named F1,F3 and F7 groups respectively).The potential molecular mechanisms behind the functions of respiratory tree and body wall were investigated by RNA-Seq.A total of 52411 expressed genes were identified from 89342 expressed transcripts.The results showed 759,254 and 334 genes were up-regulated,and 334,445 and 992 genes were down-regulated in respiratory tree of F1 vs.F3,F1 vs.F7 and F3 vs.F7,respectively.Meanwhile,2070,1601 and 896 genes were up-regulated,and 1303,1337 and 1144 genes were down-regulated in body wall between F1 vs.F3,F1 vs.F7 and F3 vs.F7,respectively.Differentially expressed genes were enriched in salivary secretion and ECM-receptor interaction pathways in respiratory tree,and in various types of N-glycan biosynthesis,ribosome and sphingolipid metabolism pathways in body wall.These results suggested respiratory tree and body wall were involved in activation of respiratory metabolisms in response to different feeding rations.Our research provided valuable knowledge for physiological differences in res-piratory metabolism. | MEI Yaoping HOU Zhishuai GAO Qinfeng DONG Shuanglin LI Xueqi XU Yuling | 2023 | Journal of Ocean University of China2023，22，6： | 0 |
| 12 | Four-inch high quality crack-free AlN layer grown on a hightemperature annealed AlN template by MOCVD显示文摘In this work,based on physical vapor deposition and high-temperature annealing(HTA),the 4-inch crack-free high-quality AlN template is initialized.Benefiting from the crystal recrystallization during the HTA process,the FWHMs of X-ray rocking curves for(002)and(102)planes are encouragingly decreased to 62 and 282 arcsec,respectively.On such an AlN template,an ultra-thin AlN with a thickness of~700 nm grown by MOCVD shows good quality,thus avoiding the epitaxial lateral over-growth(ELOG)process in which 3-4μm AlN is essential to obtain the flat surface and high crystalline quality.The 4-inch scaled wafer provides an avenue to match UVC-LED with the fabrication process of traditional GaN-based blue LED,therefore significantly improving yields and decreasing cost. | Shangfeng Liu Ye Yuan Shanshan Sheng Tao Wang Jin Zhang Lijie Huang Xiaohu Zhang Junjie Kang Wei Luo Yongde Li Houjin Wang Weiyun Wang Chuan Xiao Yaoping Liu Qi Wang Xinqiang Wang | 2021 | Journal of Semiconductors2021，42，12： | 0 |
| 13 | Functional analysis of hydrogenases and their effects on cell growth and magnetosome synthesis in Magnetospirillum gryphiswaldense显示文摘This study addressed the effect of hydrogen metabolism on cell growth and magnetosome synthesis in Magnetospirillum gryphiswaldense strain MSR-1. Two deletion mutants were generated: L206, with single deletion of the hupL gene encoding H2-uptake [NiFe] hydrogenase; and B206, with double deletion of the hyaB gene encoding H2-producing [NiFe] hydrogenase and the hupL gene. The wild-type and mutant strains were compared in terms of hydrogen uptake capability, hydrogen yield, growth rate, and iron uptake, and observed by transmission electron microscopy. Results indicate that HupSL protein is a specific H2-uptake hydrogenase while HyaAB protein is a specific H2-producing hydrogenase. In comparison to wild-type and B206, L206 released a greater quantity of H2 under conditions that induce magnetosomes synthesis, and showed higher rates of growth and iron uptake. M. gryphiswaldense appears to regulate reducing power in vivo, via H2-uptake hydrogenase and H2-producing hy- drogenase, to promote iron absorption and magnetosome synthesis. | BAN Jia JIANG Wei LI Ying ZHANG YaoPing LI JiLun | 2010 | Chinese Science Bulletin2010，55，13： | 0 |
| 14 | Combined local therapy and CAR-GPC3 T-cell therapy in advanced hepatocellular carcinoma:a proof-of-concept treatment strategy显示文摘Dear Editor,Available evidence regarding the most suitable treatment strategies for hepatocellular carcinoma(HCC)with inferior vena cava tumor thrombus(IVCTT)is extremely limited,and the median overall survival time for these patients after liver resection is only 17.76 months[1].Other local or systemic treatments for HCC with IVCTT result in a median overall survival time ranging from 5.88 to 15.36 months[1–3].Thus,new therapeutic strategies are urgently needed to improve the survival of HCC patients with IVCTT.Chimeric antigen receptor(CAR)T-cell therapy has seen success in treating B-cell neoplasms with impressive outcomes[4].However,this therapy alone has shown limited efficacy on solid tumors,such as HCC[5]. | Yaoping Shi Donghua Shi Jiachang Chi Dan Cui Xiaoyin Tang Yan Lin Siying Wang Zonghai Li Haojie Jin Bo Zhai | 2023 | Cancer Communications2023，43，9： | 0 |
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