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| 1 | Smoothelin,a new marker to determine the origin of liver fibrogenic cells显示文摘AIM:To explore this hypothesis that smooth muscle cells may be capable of acquiring a myofibroblastic phenotype,we have studied the expression of smoothelin in fibrotic conditions.METHODS:Normal liver tissue(n=3)was obtained from macroscopically normal parts of hepatectomy,taken at a distance from hemangiomas.Pathological specimens included post-burn cutaneous hypertrophic scars(n=3),fibrotic liver tissue(n=5),cirrhotic tissue(viral and alcoholic hepatitis)(n=5),and hepatocellular carcinomas(n=5).Tissue samples were fixed in 10%formalin and embedded in paraffin for immunohistochemistry or were immediately frozen in liquid nitrogen-cooled isopentane for confocal microscopy analysis.Sections were stained with antibodies against smoothelin,which is expressed exclusively by smooth muscle cells,andα-smooth muscle actin,which is expressed by both smooth muscle cells and myofibroblasts.RESULTS:In hypertrophic scars,α-smooth muscle actin was detected in vascular smooth muscle cells and in numerous myofibroblasts present in and around nodules,whereas smoothelin was exclusively expressed in vascular smooth muscle cells.In the normal liver,vascular smooth muscle cells were the only cells that expressα-smooth muscle actin and smoothelin.In fibrotic areas of the liver,myofibroblasts expressingα-smooth muscle actin were detected.Myofibroblasts co-expressingα-smooth muscle actin and smoothelin were observed,and their number was slightly increased in parallel with the degree of fibrosis(absent in liver with mild or moderate fibrosis;5%to 10%positive in liver showing severe fibrosis).In cirrhotic septa,numerous myofibroblasts co-expressedα-smooth muscle actin and smoothelin(more than 50%).In hepatocellular carcinomas,the same pattern of expression forα-smooth muscle actin and smoothelin was observed in the stroma reaction surrounding the tumor and around tumoral cell plates.In all pathological liver samples,α-smooth muscle actin and smoothelin were co-expressed in vascular smooth muscle cells.CONCLUSION:During development of advanced liver fibrosis,a subpopulation of myofibroblasts expressing smoothelin may be derived from vascular smooth muscle cells,illustrating the different cellular origins of myofibroblasts. | Sébastien Lepreux Christelle Guyot Fabrice Billet Chantal Combe Charles Balabaud Paulette Bioulac-Sage Alexis Desmoulière | 2013 | World Journal of Gastroenterology2013，19，48： | 2 |
| 2 | Activation of cul- tured rat hepatic stellate cells by tumoral hepatocytes 显示文摘 | FAOUZI S LEPREUX S BEDIN C | 1999 | Lab Invest1999，79，4： | 1 |
| 3 | Hepatic fibrosis and cirrhosis: The (myo) fibroblastic cell subpopulations involved显示文摘 | Guyot C Lepreux S Combe C | 2006 | The International Journal of Biochemistry Cell Biology2006，38，1： | 1 |
| 4 | Hepatic fibrosis and cirrho-sis:the(myo)fibroblastic cell subpopulations involved显示文摘 | Guyot C Lepreux S Combe C | 2006 | Int JBiochem Cell Biol2006，38，2： | 1 |
| 5 | Activation of cultured rat hepatic stellate cells by tumoral hepatocytes显示文摘 | Faouzi S Lepreux S Bedin C | 1999 | Lab Invest1999，79，4： | 1 |
| 6 | Association of adenoma and focal nodular hyperplasia:experience of a single French academic center显示文摘 | Laurent C Trillaud H Lepreux S | 2003 | Comp Hepatol2003，2，1： | 1 |
| 7 | Hepatic fibrosis and cirrhosis:the (myo) fibroblastic cell subpopulations involved显示文摘 | Guyot C Lepreux S Combea C | | 0，，02： | 1 |
| 8 | Activation of cultured rat hepatic stellate cells by tumoral hepatocytes显示文摘 | Faouzi S Lepreux S Bedin C | 1999 | Lab Invest1999，79，4： | 1 |
| 9 | Hepatic fibrosis and cirrhosis the(myo)fibroblastic cell subpopulations in-volved 显示文摘 | Guyot C Lepreux S Combe C | 2006 | Int J Biochem Cell Biol2006，38，2： | 1 |
| 10 | Severe linear form of granuloma annulare along Blaschko's lines preceding the onset of a classical form of granuloma annulare in a child显示文摘 | Morice-Picard F Boralevi F Lepreux S | 2007 | Br J Dermatol2007，157，5： | 1 |
| 11 | Hepatic fibrosis and cir- rhosis: the (myo)fibroblastic cell subpopulations involved 显示文摘 | Guyot C Lepreux S Combe C | 2006 | Int J Biochem Cell Biol2006，38，2： | 1 |
| 12 | Melanocyte detachment after skin friction in non lesional skin of patients with generalized vitiligo显示文摘 | Gauthier Y Cario-Andre M Lepreux S | 2003 | Br J Dermatol2003，148，1： | 1 |
| 13 | Hepatic fibrosis and cirrhosis the (myo)fibroblastic cell subpopulations involved显示文摘 | Guyot C Lepreux S Combe C | 2006 | Int J Biochem Cell Biol2006，38，2： | 1 |
| 14 | Expression and cellular localization of fibrillin-1 in normal and pathological human liver显示文摘 | Liliane Dubuisson Sébastien Lepreux Paulette Bioulac-Sage Charles Balabaud Andréa Monte Alto Costa Jean Rosenbaum Alexis Desmoulière | 2001 | Journal of Hepatology2001，，4： | 1 |
| 15 | Hepatic fibrosis and cirrhosis: the (myo)fibroblastic cell subpopulations in- volved 显示文摘 | Guyot C Lepreux S Combe C | 2006 | International Journal of Biochemistry and Cell Biology2006，38，2： | 1 |
| 16 | Activation of cultured rat hepatic stellate cells by tumoral hepatocytes显示文摘 | Faouzi S Lepreuxs Bedin C | 1999 | Lab invest1999，79，： | 1 |
| 17 | Mutation of TP53 gene is involved in carcinogenesis of hepatic undifferentiated (embryonal) sarcoma of the adult in contrast with Wnt or telomerase pathways : animmunohistochemical study of three cases with genomic relation in two cases 显示文摘 | Lepreux S Rebouissou S Le Bail B | 2005 | J Hepato12005，42，3： | 1 |
| 18 | Quantitative elastography of renal transplants using supersonic shear imaging: a pilot study显示文摘 | Grenier N Poulain S Lepreux S | 2012 | Eur Radiol2012，22，10： | 1 |
| 19 | Hepatic fibrosis and cirrhosis:The (myo)fibroblastic cell subpopulations involved显示文摘 | Guyot C Lepreux S Combe C | 2006 | Int J Biochem Cell Biol2006，38，2： | 1 |
| 20 | Clinical, Morphologic, and Molecular Features Defining So-Called Telangiectatic Focal Nodular Hyperplasias of the Liver显示文摘 | Paulette Bioulac-Sage Sandra Rebouissou Antonio Sa Cunha Emmanuelle Jeannot Sébastien Lepreux Jean-Frédéric Blanc Hélène Blanché Brigitte le Bail Jean Saric Pierre Laurent-Puig Charles Balabaud Jessica Zucman-Rossi | 2005 | Gastroenterology2005，，5： | 1 |
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