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| 1 | Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System显示文摘Transcription activator-like effector nucleases(TALENs) and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) systems have emerged as powerful tools for genome editing in a variety of species. Here, we report, for the first time, targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system. We designed five TALENs targeting 4 genes, namely ZmPDS, ZmIPK1A, ZmIPK, ZmMRP4, and obtained targeting efficiencies of up to 23.1% in protoplasts, and about 13.3% to 39.1% of the transgenic plants were somatic mutations. Also, we constructed two gRNAs targeting the ZmIPK gene in maize protoplasts, at frequencies of 16.4% and 19.1%, respectively. In addition, the CRISPR/Cas system induced targeted mutations in Z. mays protoplasts with efficiencies(13.1%) similar to those obtained with TALENs(9.1%). Our results show that both TALENs and the CRISPR/Cas system can be used for genome modification in maize. | Zhen Liang Kang Zhang Kunling Chen Caixia Gao | 2014 | Journal of Genetics and Genomics2014，41，2： | 136 |
| 2 | Diagnosis, treatment, and prevention of 2019 novel coronavirus infection in children: experts' consensus statement显示文摘Since the outbreak of 2019 novel coronavirus infection (2019-nCoV) in Wuhan City,China,by January 30,2020,a total of 9692 confirmed cases and 15,238 suspected cases have been reported around 31 provinces or cities in China.Among the confirmed cases,1527 were severe cases,171 had recovered and been discharged at home,and 213 died.And among these cases,a total of 28 children aged from 1 month to 17 years have been reported in China.For standardizing prevention and management of 2019-nCoV infections in children,we called up an experts' committee to formulate this experts' consensus statement.This statement is based on the Novel Coronavirus Infection Pneumonia Diagnosis and Treatment Standards (the fourth edition) (National Health Committee) and other previous diagnosis and treatment strategies for pediatric virus infections.The present consensus statement summarizes current strategies on diagnosis,treatment,and prevention of 2019-nCoV infection in children. | Kunling Shen Yonghong Yang Tianyou Wang Dongchi Zhao Yi Jiang Runming Jin Yuejie Zheng Baoping Xu Zhengde Xie Likai Lin Yunxiao Shang Xiaoxia Lu Sainan Shu Yan Bai Jikui Deng Min Lu Leping Ye Xuefeng Wang Yongyan Wang Liwei Gao | 2020 | World Journal of Pediatrics2020，16，3： | 56 |
| 3 | Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs显示文摘 | Qiwei Shan Yanpeng Wang Kunling Chen Zhen Liang Jun Li Yi Zhang Kang Zhang Jinxing Liu Daniel F. Voytas Xuelian Zheng Yong Zhang Caixia Gao | 2013 | Molecular Plant2013，6，4： | 37 |
| 4 | TALENs:Customizable Molecular DNA Scissors for Genome Engineering of Plants显示文摘Precise genome modification with engineered nucleases is a powerful tool for studying basic biology and applied biotechnology. Transcription activator-like effector nucleases(TALENs),consisting of an engineered specific(TALE) DNA binding domain and a Fok I cleavage domain,are newly developed versatile reagents for genome engineering in different organisms.Because of the simplicity of the DNA recognition code and their modular assembly,TALENs can act as customizable molecular DNA scissors inducing double-strand breaks(DSBs) at given genomic location.Thus,they provide a valuable approach to targeted genome modifications such as mutations, insertions,replacements or chromosome rearrangements.In this article,we review the development of TALENs,and summarize the principles and tools for TALEN-mediated gene targeting in plant cells,as well as current and potential strategies for use in plant research and crop improvement. | Kunling Chen Caixia Gao | 2013 | Journal of Genetics and Genomics2013，40，6： | 18 |
| 5 | An Uncanonical CCCH-Tandem Zinc-Finger Protein Represses Secondary Wall Synthesis and Controls Mechanical Strength in Rice显示文摘第二等的墙,代表生物资源的体积,在植物生长和改编上有大影响到环境。第二等的墙合成被一个复杂发信号 transduction 网络交换并且调整。然而,有这些规章的小径的有限理解。这里,我们报导(IIP4 )交往 ILA1 蛋白质 4 能镇压第二等的墙合成。IIP4 是象 Raf 一样 MAPKKK 的 phosphorylation 底层,但是它的功能是未知的。由产生 iip4 异种和相关转基因的植物,我们发现在 IIP4 的损害提高第二等的墙形成。基因表示和 transactivation 活动试金表明 IIP4 否定地调整 MYB61 和 CESA 的表示但是不绑 ? 他们的倡导者。IIP4 与 NAC29/NAC31 交往,第二等的墙合成的在上游的管理者,并且在植物压制下游地规章的小径。显示出的 Mutagenesis 分析那 phosphomimic IIP4 从原子核的蛋白质 translocate 到细胞质。而且,我们表明 IIP evolutionarily 被保存并且分享 unreported CCCH 主题,叫作 uncanonical CCCH 双人脚踏车锌手指蛋白质。一起,我们的学习提供机械学的卓见进第二等的墙合成的控制并且为在庄稼改进相关农学的特点介绍一个机会。 | Dongmei Zhang Zuopeng Xu Shaoxue Cao Kunling Chen Shance Li Xiangling Liu Caixia Gao Baocai Zhang Yihua Zhou | 2018 | Molecular Plant2018，11，1： | 14 |
| 6 | Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene显示文摘Maize(Zea mays L.)is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013(Baldaufa et al.,2016).Heterosis is widely used to increase the productivity of maize,and the first commercial hybrid maize was introduced in the 1930s(Duvick,2001).The high-yield maize varieties used today are all hybrids,and the production of hybrid maize seed requires sterilization to prevent self-pollination.Conventional seed produc- | Jun Li Huawei Zhang Xiaomin Si Youhui Tian Kunling Chen Jinxing Liu Huabang Chen Caixia Gao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 7 | effective treatment of post-intubation subglottic stenosis in children with holmium laser therapy and cryotherapy via flexible bronchoscopy显示文摘Importance:Post-intubation subglottic stenosis(SGS)in children can be life threatening.Definitive treatment varies and lacks a universally accepted approach.Objective:We performed a prospective study to assess the safety and feasibility of holmium laser combined with cryotherapy delivered via flexible bronchoscopy for the treatment of post-intubation SGS in children.Methods:This study involved all patients with post-intubation SGS seen at the Interventional Pulmonology Department of Beijing Children's Hospital between July 2014 and December 2016.Holmium laser treatment and cryotherapy was then performed under flexible bronchoscopy,whose parents refused to accept the alternative standard treatment of tracheotomy and balloon dilation under direct laryngoscopy.results:Sixteen patients with post-intubation SGS were included in this study.Ages ranged from 2 months to 12.25 years old.According to the Cotton-Myer grading system,three cases were Grade II,12 cases were Grade III,and one case was Grade IV.According to the McCaffrey system,eight cases were Stage 1,two cases were Stage 2,and six cases were Stage 3.The average number of procedures was 4.88.Fifteen of the 16 patients achieved clinical cure.One patient achieved clinical improvement.The average treatment course duration was 55.31 days.No severe complications were seen.Post-treatment clinical symptoms,endoscopic findings and quality of life showed marked improvement.Interpretation:Our study supports the conclusion that holmium laser treatment combined with cryotherapy via flexible bronchoscopy appears to be a safe and feasible treatment for post-intubation SGS in children. | Anxia Jiao Fang Liu Andrew D.Lerner Xiaochun Rao Yan Guo Chenfang Meng Yuena Pan Gan Li Zheng Li Fang Wang Jing Zhao Yuyan Ma Xicheng Liu Xin Ni Kunling Shen | 2019 | Pediatric Investigation2019，3，1： | 10 |
| 8 | Cloning and expression of two sterol C-24 methyltransferase genes from upland cotton(Gossypium hirsuturm L.)显示文摘Brassinosteroids (BRs) are an important class of plant steroidal hormones that are essential in a wide variety of physiological proc-esses. Two kinds of intermediates,sitosterol and campesterol,play a crucial role in cell elongation,cellulose biosynthesis,and accumula-tion. To illuminate the effects of sitosterol and campesterol on the development of cotton (Gossypium hirsuturm L.) fibers through screening cotton fiber EST database and contigging the candidate ESTs,two key genes GhSMT2-1 and GhSMT2-2 controlling the sitos-terol biosynthesis were cloned from developing fibers of upland cotton cv. Xuzhou 142. The full length of GhSMT2-1 was 1,151 bp,in-cluding an 8 bp 5′-untranslated region (UTR),a 1,086 bp open reading frame (ORF),and a 57 bp 3′-UTR. GhSMT2-1 gene encoded a polypeptide of 361 amino acid residues with a predicted molecular mass of 40 kDa. The full length of GhSMT2-2 was 1,166 bp,including an 18 bp 5′-UTR,a 1,086 bp ORF,and a 62 bp 3′-UTR. GhSMT2-2 gene encoded a polypeptide of 361 amino acid residues with a pre-dicted molecular mass of 40 kDa. The two deduced amino acid sequences had high homology with the SMT2 from Arabidopsis thaliana and Nicotiana tabacum. Furthermore,the typical conserved structures characterized by the sterol C-24 methyltransferase,such as region I (LDVGCGVGGPMRAI),region Ⅱ (IEATCHAP),and region Ⅲ (YEWGWGQSFHF),were present in both deduced proteins. Southern blotting analysis indicated that GhSMT2-1 or GhSMT2-2 was a single copy in upland cotton genome. Quantitative real-time RT-PCR analysis revealed that the highest expression levels of both genes were detected in 10 DPA (day post anthesis) fibers,while the lowest levels were observed in cotyledon and leaves. The expression level of GhSMT2-1 was 10 times higher than that of GhSMT2-2 in all the organs and tissues detected. These results indicate that the homologue of sterol C-24 methyltransferase gene was cloned from upland cotton and both GhSMT2 genes play a crucial role in fiber elongation. The role of GhSMT2-1 may be more important than that of GhSMT2-2. | Ming Luo,Kunling Tan,Zhongyi Xiao,Mingyu Hu,Peng Liao,Kuijun Chen Key Laboratory of Biotechnology and Crop Quality Improvement,Ministry of Agriculture Biotechnology Research Center,Southwest University,Chongqing 400716,China | 2008 | Journal of Genetics and Genomics2008，35，6： | 8 |
| 9 | A Multicenter Study of Viral Aetiology of Community-Acquired Pneumonia in Hospitalized Children in Chinese Mainland显示文摘Community-acquired pneumonia(CAP) is one of the leading causes of morbidity and mortality in children worldwide.In this study,we aimed to describe the aetiology of viral infection of pediatric CAP in Chinese mainland.During November2014 to June 2016,the prospective study was conducted in 13 hospitals.The hospitalized children under 18 years old who met the criteria for CAP were enrolled.The throat swabs or nasopharyngeal aspirates(NPAs) were collected which were then screened 18 respiratory viruses using multiplex PCR assay.Viral pathogens were present in 56.6%(1539/2721) of the enrolled cases,with the detection rate of single virus in 39.8% of the cases and multiple viruses in 16.8% of the cases.The most frequently detected virus was respiratory syncytial virus(RSV)(15.2%,414/2721).The highest detection rate of virus was in <6-month-age group(70.7%,292/413).RSV,human metapneumovirus(HMPV),human parainfluenza viruses(HPIVs) and influenza B virus(Flu B) showed the similar prevalence patterns both in north and south China,but HPIVs,Flu A,human bocavirus(HBoV),human adenovirus(HAdV) and human coronaviruses(HCoVs) showed the distinct circulating patterns in north and south China.Human enterovirus/human rhinovirus(HEV/HRV)(27.6%,27/98),HBoV(18.4%,18/98),RSV(16.3%,16/98) and HMPV(14.3%,14/98) were the most commonly detected viruses in severe pneumonia cases with single virus infection.In conclusion,viral pathogens are frequently detected in pediatric CAP cases and may therefore play a vital role in the aetiology of CAP.RSV was the most important virus in hospitalized children with CAP in Chinese mainland. | Yun Zhu Baoping Xu Changchong Li Zhimin Chen Ling Cao Zhou Fu Yunxiao Shang Aihuan Chen Li Deng Yixiao Bao Yun Sun Limin Ning Shuilian Yu Fang Gu Chunyan Liu Ju Yin Adong Shen Zhengde Xie Kunling Shen | 2021 | Virologica Sinica2021，36，6： | 7 |
| 10 | Enhanced antibacterial behavior of a novel Cu-bearing high-entropy alloy显示文摘Contact infection of bacteria and viruses has been a critical threat to human health. The worldwideoutbreak of COVID-19 put forward urgent requirements for the research and development of the selfantibacterial materials, especially the antibacterial alloys. Based on the concept of high-entropy alloys, thepresent work designed and prepared a novel Co_(0.4)FeCr_(0.9)Cu_(0.3) antibacterial high-entropy alloy with superior antibacterial properties without intricate or rigorous annealing processes, which outperform the antibacterial stainless steels. The antibacterial tests presented a 99.97% antibacterial rate against Escherichiacoli and a 99.96% antibacterial rate against Staphylococcus aureus after 24 h. In contrast, the classic antibacterial copper-bearing stainless steel only performed the 71.50% and 80.84% antibacterial rate, respectively. The results of the reactive oxygen species analysis indicated that the copper ion release and theimmediate contact with copper-rich phase had a synergistic effect in enhancing antibacterial properties.Moreover, this alloy exhibited excellent corrosion resistance when compared with the classic antibacterialstainless steels, and the compression test indicated the yield strength of the alloy was 1015 MPa. Thesefindings generate fresh insights into guiding the designs of structure-function-integrated antibacterial alloys. | Guangyu Ren Lili Huang Kunling Hu Tianxin Li Yiping Lu Dongxu Qiao Haitao Zhang Dake Xu Tongmin Wang Tingju Li Peter K.Liaw | 2022 | Journal of Materials Science & Technology2022，，22： | 6 |
| 11 | Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation显示文摘Phytosterols play an important role in plant growth and development,including cell division,cell elongation,embryogenesis,cellulose biosynthesis,and cell wall formation.Cotton fiber,which undergoes synchronous cell elongation and a large amount of cellulose synthesis,is an ideal model for the study of plant cell elongation and cell wall biogenesis.The role of phytosterols in fiber growth was investigated by treating the fibers with tridemorph,a sterol biosynthetic inhibitor.The inhibition of phytosterol biosynthesis resulted in an apparent suppression of fiber elongation in vitro or in planta.The determination of phytosterol quantity indicated that sitosterol and campesterol were the major phytosterols in cotton fibers;moreover,higher concentrations of these phytosterols were observed during the period of rapid elongation of fibers.Furthermore,the decrease and increase in campesterol:sitosterol ratio was associated with the increase and decease in speed of elongation,respectively,during the elongation stage.The increase in the ratio was associated with the transition from cell elongation to secondary cell wall synthesis.In addition,a number of phytosterol biosynthetic genes were down-regulated in the short fibers of ligon lintless-1mutant,compared to its near-isogenic wild-type TM-1.These results demonstrated that phytosterols play a crucial role in cotton fiber development,and particularly in fiber elongation. | Shasha Deng Ting Wei Kunling Tan Mingyu Hu Fang Li Yunlan Zhai Shue Ye Yuehua Xiao Lei Hou Yan Pei Ming Luo | 2016 | Science China(Life Sciences)2016，59，2： | 5 |
| 12 | Type I interferon:From innate response to treatment for COVID-19显示文摘Effective prophylactic and therapeutic interventions are urgentlyneeded to address the coronavirus disease 2019(COVID-19)pandemic.Various antiviral drugs have recently been tested.Type I interferon(IFN)is a regulatory protein involved in the innate immune response,with broad-spectrum antiviral activities and the ability to directly block viral replication and support the immune response to eliminate virus infection.Insufficient virus-induced type I IFN production is characteristic of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection,because SARS-CoV-2 suppresses the IFN response by interacting with essential IFN signaling pathways.Exogenous type I IFN is recommended for treating COVID-19.Unexpectedly however,angiotensin converting enzyme-2(ACE2)receptor,which acts as a SARS-CoV-2 receptor,was shown to be stimulated by IFN,raising doubts about the suitability of IFN use.However,further studies have excluded concerns regarding IFN administration.Type I IFNs,including IFN-α1b,have been used clinically as antiviral drugs for many years and have shown strong antiviral activity against SARS-CoV-2in vitro.Preliminary clinical studies of type I IFNs,especially when delivered via aerosol inhalation,have demonstrated efficacy for the treatment and prevention of COVID-19.Randomized controlled trials of IFN for COVID-19 treatment are ongoing. | Fuyu Lin Kunling Shen | 2020 | Pediatric Investigation2020，4，4： | 5 |
| 13 | Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing显示文摘Herbicide-tolerant rice varieties generated by genome editing are highly desirable for weed control.We have used a cytosine base editor to create a series of missense mutations in the P171 and/or G628 codons of the acetolactate synthase(ALS)gene to confer herbicide tolerance in rice.The four different missense mutations in the P171 codon,P171S,P171A,P171Yand P171F,exhibited different patterns of tolerance towards five representative herbicides from five chemical families of ALS inhibitors.For example,P171S and P171A had lower levels of tolerance than P171Y and P171F to bispyribac but not to the other herbicides.Interestingly,a novel triple mutant(P171F/G628E/G629S)had the highest tolerance to all five tested herbicides.Field trials showed that both P171F and P171F/G628E/G629S could potentially be used with nicosulfuron.Our work illustrates an effective way of using base editing to generate herbicide tolerance in elite rice varieties. | Rui Zhang Sha Chen Xiangbing Meng Zhuangzhuang Chai Delin Wang Yuge Yuan Kunling Chen Linjian Jiang Jiayang Li Caixia Gao | 2021 | Science China(Life Sciences)2021，64，10： | 4 |
| 14 | Transient expression of a TaGRF4-TaGIF1 complex stimulates wheat regeneration and improves genome editing显示文摘Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1(TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site of TaGRF4, the resulting complex(mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of m TaGRF4-TaGIF1 together with a cytosine base editor targeting TaALS resulted in 2-9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, m TaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation. | Fengti Qiu Sinian Xing Chenxiao Xue Jinxing Liu Kunling Chen Tuanyao Chai Caixia Gao | 2022 | Science China(Life Sciences)2022，65，4： | 4 |
| 15 | Molecular and Functional Characterization of Sphingosine-1-Phosphate Lyase Homolog from Higher Plants显示文摘Sphingosine-1-phosphate lyase (系统程序设计语言) 涉及降级表明分子 sph-ingosine-1-phosphate 的 conservedsphingolipid。从 Arabidopsis thaliana 和米饭的通常认为的系统程序设计语言蛋白质的生物信息、分子、功能的分析(指定了为 AtSPL 和 OsSPL,分别地) 被进行。氨基酸顺序比较表明植物系统程序设计语言包含了 thepyridoxal 依赖的脱羧酶领域和可以涉及底层催化作用的保存残余。当在 Saccharomyces cerevisiae 表示了时, AtSPL 和 OsSPL 改正了酵母 dpl1 删除紧张的过分敏感的显型,它在内长的系统程序设计语言活动是缺乏的,到长链基于的外长的 sphingolipid (LCB ) ,建议那植物系统程序设计语言蛋白质是在降级的 phosphorylated LCB 的功能的 invivo。在 A。thaliana, AtSPL 抄本在根,茎,叶子,花和 siliques 被检测。在 pAtSPL-AtSPL::GUS 转基因的线, AtSPL::GUS 熔化蛋白质在许多植物、繁殖的纸巾被发现。AtSPL 表示水平动态地在叶开发和老朽期间被调整,并且稳定地并且显著地在 A 被增加。thaliana 幼苗在 A 与房间对待系统程序设计语言的导致死亡的真菌的毒素 fumonisin B1.The 潜在的功能。thaliana 被讨论…… | Yan Niu Kunling Chen Jizhou Wang Xin Liu Huanju Qin Aimin Zhang Daowen Wang | 2007 | Journal of Integrative Plant Biology2007，49，3： | 3 |
| 16 | Safety and efficacy of Yupingfeng granules in children with recurrent respiratory tract infection:A randomized clinical trial显示文摘Importance:Recurrent respiratory tract infection(RRTI)is common in children.Inappropriate RRTI treatment will lead to asthma and other diseases,thereby seriously affecting the growth and physical health of children.Immune function modulation can prevent and alleviate childhood RRTI.Yupingfeng(YPF),a patented traditional Chinese medicine(TCM),has immunomodulatory effects and is widely used in China to treat children with RRTI.Objective:To evaluate the safety and efficacy of YPF monotherapy in treating children with RRTI.Methods:This multicenter,randomized,double-blind,double-simulation,noninferiority clinical trial was conducted from January 2015 to August 2017,with an 8-week treatment period and 52-week follow-up after the drug withdrawal.Children aged 2–6 years with RRTI meeting the inclusion and exclusion criteria were enrolled in 13 hospitals in China and divided randomly into three groups(2:2:1 ratio)to receive YPF,pidotimod,or placebo.The primary outcome was the proportion of RRTI returning to normal standard level during the follow-up.The secondary outcomes were reduction in the number of RRTI recurrences,effect on clinical symptoms(in accord with TCM practice),effect per symptom,and safety.The trial was registered at the Chinese Clinical Trials Registry(www.chictr.org.cn)under the unique identifier ChiCTR-IPR-15006847.Results:Three hundred and fifty-one children were enrolled and randomly assigned to 3 groups;124,125,and 61 children in the YPF,pidotimod,and placebo groups,respectively,had completed the trial.During the follow-up,the proportion of RRTI returning to normal standard level was 73.13%,67.15%,and 38.81%with YPF,pidotimod,and placebo,respectively(P<0.0001).The proportion of cases who returned to normal standard level in the YPF group was 34.32%higher than that in the placebo group.The safety profile did not significantly differ among the groups.Interpretation:YPF granules were noninferior to the active control drug pidotimod oral solution for the treatment of RRTI in children,and were superior to placebo,with a high safety profile. | Baoping Xu Xinmin Li Siyuan Hu Yixiao Bao Fengmei Chen Zhimin Chen Yonggang Du Enmei Liu Yufeng Liu Qinghui Mou Baoling Su Bo Wang Jianwen Xu Guiping Xu Qiaozhi Yang Liwei Gao Xiaohui Liu Lei Li Rong Ma Kunling Shen | 2022 | Pediatric Investigation2022，6，2： | 3 |
| 17 | Severe acute respiratory syndrome coronavirus 2-induced multisystem inflammatory syndrome in children显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)-induced multisystem inflammatory syndrome in children(MIS-C)is a life-threatening illness that has been reported in the United States and Europe.It affects multiple organ systems and often requires patient admission to an intensive care unit.Although some features of MIS-C overlap with Kawasaki disease,MIS-C is more common among older children and adolescents,more often affects cardiovascular and gastrointestinal systems,and more frequently presents with elevated inflammatory markers.Rapid and complete clinical recovery is possible in nearly all patients following immunomodulation therapy.Thus far,MIS-C pathophysiology and long-term prognosis are not sufficiently clear;further studies are needed. | Zhiguan Feng Yanmin Bao Yonghong Yang Yuejie Zheng Kunling Shen | 2020 | Pediatric Investigation2020，4，4： | 3 |
| 18 | Preliminary study of microstructure, mechanical properties and corrosion resistance of antibacterial Ti-15Zr-xCu alloy for dental application显示文摘Ti-15 Zr-xCu(3≤x≤7,wt.%) novel antibacterial and antibiofilm alloys with competitive mechanical properties,biological responses and corrosion resistance were designed and fabricated.Annealing heat treatment on Ti-15 Zr-7 Cu(TZC-7 A),after holding for 2 h at slightly above their beta transus temperature(BTT) ensured their tensile strength(UTS),yield strength(YS) and hardness(HRV) were improved by31.2%,20% and 12.3% respectively compared to the control without Cu,Ti-15 Zr(T-15 ZA).Although the3 wt.% Cu alloy displayed the highest elongation(26%),the TZC-7 A alloy also possessed a good ductility.Presence of evenly dispersed Ti2 Cu and Zr2 Cu Cu-rich intermetallic phases formed as interwoven and alternating lamellae within the α+β matrix as a result of Cu addition,as revealed by X-ray diffraction(XRD),scanning electron microscopy(SEM) and transmission electron microscopy(TEM).These greatly contributed to their strengthening and bactericidal properties.Over 98% antibacterial effect against E.coli and S.aureus have been imparted,coupled with excellent biofilm inhibition.Potentiodynamic polarization curves showed that the TZC-7 A alloy possessed higher corrosion resistance than commercially pure titanium,cp-Ti;contact angle test revealed enhanced hydrophilicity;while confocal laser scanning microscopy(CLSM) and cell counting kit(CCK-8) assays also displayed drastically lowered bacterial adhesion rate with comparatively no cytotoxicity.Cell attachment on all alloys was similar but the best spread was obtained on TZC-7 A after 24 h.The developed alloy has good potential as an antibacterial implant material with combination of optimized properties. | Sharafadeen Kunle Kolawole Wang Hai Shuyuan Zhang Ziqing Sun Muhammad Ali Siddiqui Ihsan Ullah Wei Song Frank Witte Ke Yang | 2020 | Journal of Materials Science & Technology2020，47，15： | 2 |
| 19 | Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1)to nick the target DNA strand显示文摘The length of the sgRNA-DNA complementary sequence is a key factor influencing the cleavage activity of Streptococcus pyogenes Cas9(SpCas9)and its variants.The detailed mechanism remains unknown.Here,based on in vitro cleavage assays and base editing analysis,we demonstrate that reducing the length of this complementary region can confer nickase activity on SpCas9 and eSpCas9(1.1).We also show that these nicks are made on the target DNA strand.These properties encouraged us to develop a dual-functional system that simultaneously carries out double-strand DNA cleavage and C-to-T base conversions at separate targets.This system provides a novel tool for achieving trait stacking in plants. | Rong Fan Zhuangzhuang Chai Sinian Xing Kunling Chen Fengti Qiu Tuanyao Chai Jin-Long Qiu Zhengbin Zhang Huawei Zhang Caixia Gao | 2020 | Science China(Life Sciences)2020，63，11： | 2 |
| 20 | Cloning and expression analysis of GhDET3, a vacuolar H^+-ATPase subunit C gene, from cotton显示文摘Vacuolar H+-ATPase was regarded as a key enzyme promoting the fiber cell elongation in cotton (Gossypium hirsuturm L.) through regulating turgor-driven pressure involved in polarity expansion of single cell fiber. The DET3, a V-ATPase subunit C, plays an impor- tant role in assembling subunits and regulating the enzyme activity, and is involved in Brassinosteroid-induced cell elongation. To ana- lyze the function of GhDET3 on the elongation of cotton fibers, seven candidates of ESTs were screened and contigged for a 5′-upstream sequence, and the 3′-RACE technique was used to clone the 3′-downstream sequence for the full length of GhDET3 gene. The full length of the target clone was 1,340 bp, including a 10 bp 5′-UTR, an ORF of 1,134 bp, and a 196 bp 3′-UTR. This cDNA sequence encoded a polypepide of 377 amino acid residues with a predicted molecular mass of 43 kDa and a basic isoelectric point of 5.58. Furthermore, a length of 3,410 bp sequence from genomic DNA of GhDET3 was also cloned by PCR. The deduced amino acid sequence had a high ho- mology with DET3 from Arabidopsis, rice, and maize. Quantitative real-time PCR (qRT-PCR) analysis showed that the GhDET3 expres- sion pattern was ubiquitous in all the tissues and organs detected. The result also revealed that the accumulation of GhDET3 mRNA reached the highest profile at the fiber elongation stage in 12 DPA (days post anthesis) fibers, compared with the lowest level at the fiber initiation stage in 0 DPA ovules (with fibers). The transcript accumulation in fibers and ovules shared the similar variation tendency. In addition, in vitro ovule culture experiment demonstrated that exogenous 24-EBL treatment to 4 DPA ovules (with fibers) was capable of increasing the expression level of GhDET3, and the mRNA accumulation of GhDET3 increased in transgenic FBP7::GhDET2 cotton fibers in vivo. These results indicate that GhDET3 gene plays a crucial role in cotton fiber elongation. | Zhongyi Xiao, Kunling Tan, Mingyu Hu, Peng Liao, Kuijun Chen, Ming Luo* Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture Biotechnology Research Center, Southwest University, Chongqing 400716, China | 2008 | Journal of Genetics and Genomics2008，35，5： | 2 |
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