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| 1 | Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing显示文摘COVID-19,caused by SARS-CoV-2[1,2],has been contained in China through stringent non-pharmaceutical interventions.Border control and quarantine have effectively prevented the virus from being spread by infected travellers,but the risk of resurgence caused by other routes of introduction and transmission remains unclear,and current strategies to prevent resurgence could be flawed.Since July,SARS-CoV-2 RNA contaminations in frozen food imported from countries with ongoing epidemics have been reported in nine provinces in China[3,4].However,there is no robust evidence of COVID-19 outbreaks initiated by environmentto-human transmission.Here we add to evidence of such transmission by investigating the recent COVID-19 resurgence in Beijing. | Xinghuo Pang Lili Ren Shuangsheng Wu Wentai Ma Jian Yang Lin Di Jie Li Yan Xiao Lu Kang Shichang Du Jing Du Jing Wang Gang Li Shuguang Zhai Lijuan Chen Wenxiong Zhou Shengjie Lai Lei Gao Yang Pan Quanyi Wang Mingkun Li Jianbin Wang Yanyi Huang Jianwei Wang COVID-19 Field Response Group COVID-19 Laboratory Testing Group | 2020 | National Science Review2020，7，12： | 51 |
| 2 | Effects of Tempering Temperature and Mo/Ni on Microstructures and Properties of Lath Martensitic Wear-Resistant Steels显示文摘The tempering behavior was experimentally studied in lath martensitic wear-resistant steels with various Mo/Ni contents after tempering at different temperatures from 200to 600℃.It is shown that a good combination of hardness(HV)(420-450)and-20℃impact toughness(38-70J)can be obtained after quenching and tempering at 200-250 ℃.The microstructure at this temperature is lath structure with rod-like and/or flake-likeε-carbide with about 10nm in width and 100nm in length in the matrix,and the fracture mechanism is quasi-cleavage fracture combining with ductile fracture.Tempering at temperature from 300to 400℃results in the primary quasi-cleavagefracture due to the carbide transformation from resolved retained austenite and impurity segregation between laths or blocks.However,when the tempering temperature is higher than 500℃,the hardness(HV)is lower than 330 and the fracture mechanism changes to ductile fracture due to the spheroidization and coarsening of cementite.Additions of Mo and Ni have no significant effects on the carbides morphologies at low tempering temperatures,but improve the resistance to softening and embrittling for steels when tempered at above 350℃. | CAO Yi WANG Zhao-dong KANG Jian WU Di WANG Guo-dong | 2013 | Journal of Iron and Steel Research(International)2013，20，4： | 13 |
| 3 | Regression model to estimate flood impact on corn yield using MODIS NDVI and USDA cropland data layer显示文摘Flood events and their impact on crops are extremely significant scientific research issues; however,flood monitoring is an exceedingly complicated process. Flood damages on crops are directly related to yield change,which requires accurate assessment to quantify the damages. Various remote sensing products and indices have been used in the past for this purpose. This paper utilizes the moderate resolution imaging spectroradiometer(MODIS) weekly normalized difference vegetation index(NDVI) product to detect and further quantify flood damages on corn within the major corn producing states in the Midwest region of the US. County-level analyses were performed by taking weighted average of all pure corn pixels(>90%) masked by the United States Department of Agriculture(USDA) Cropland Data Layer(CDL). The NDVI-based time-series difference between flood years and normal year(median of years 2000–2014) was used to detect flood occurrences. To further measure the impact of the flood on corn yield,regression analysis between change in NDVI and change in corn yield as independent and dependent variables respectively was performed for 30 different flooding events within growing seasons of the corn. With the R2 value of 0.85,the model indicates statistically significant linear relation between the NDVI and corn yield. Testing the predictability of the model with 10 new cases,the average relative error of the model was only 4.47%. Furthermore,small error(4.8%) of leave-one-out cross validation(LOOCV) along with smaller statistical error indicators(root mean square error(RMSE),mean absolute error(MAE),and mean bias error(MBE)),further validated the accuracy of the model. Utilizing the linear regression approach,change in NDVI during the growing season of corn appeared to be a good indicator to quantify the yield loss due to flood. Additionally,with the 250 m MODIS-based NDVI,these yield losses can be estimated up to field level. | Ran jay Shrestha Liping Di Eugene G. Yu Lingjun Kang SHAO Yuan-zhen BAI Yu-qi | 2017 | Journal of Integrative Agriculture2017，16，2： | 8 |
| 4 | A deep learning-integrated micro-CT image analysis pipeline for quantifying rice lodging resistance-related traits显示文摘Lodging is a common problemin rice,reducing its yield andmechanical harvesting efficiency.Rice architecture is a key aspect of its domestication and a major factor that limits its high productivity.The ideal rice culm structure,includingmajor_axis_culm,minor axis_culm,andwall thickness_culm,is critical for improving lodging resistance.However,the traditionalmethod ofmeasuring rice culms is destructive,time consuming,and labor intensive.In this study,we used a high-throughput micro-CT-RGB imaging system and deep learning(SegNet)todevelopa high-throughputmicro-CTimageanalysis pipelinethatcanextract 24 riceculmmorphological traits and lodging resistance-related traits.When manual and automatic measurements were compared at themature stage,the mean absolute percentage errors for major_axis_culm,minor_axis_culm,andwall_thickness_culmin 104 indica rice accessionswere 6.03%,5.60%,and 9.85%,respectively,and the R^(2) valueswere 0.799,0.818,and 0.623.We also builtmodels of bending stress using culmtraits at the mature and tillering stages,and the R^(2) values were 0.722 and 0.544,respectively.The modeling results indicated that this method can quantify lodging resistance nondestructively,even at an early growth stage.In addition,we also evaluated the relationships of bending stress toshoot dryweight,culm density,and drought-related traits and found that plants with greater resistance to bending stress had slightly higher biomass,culm density,and culm area but poorer drought resistance.In conclusion,we developed a deep learning-integrated micro-CT image analysis pipeline to accurately quantify the phenotypic traits of rice culms in4.6 min per plant;this pipeline will assist in future high-throughput screening of large rice populations for lodging resistance. | Di Wu Dan Wu Hui Feng Lingfeng Duan Guoxing Dai Xiao Liu Kang Wang Peng Yang Guoxing Chen Alan P.Gay John H.Doonan Zhiyou Niu Lizhong Xiong Wanneng Yang | 2021 | Plant Communications2021，2，2： | 7 |
| 5 | Texture and stretch formability of rolled Mg–Zn–RE(Y, Ce, and Gd) alloys at room temperature显示文摘To develop a new magnesium alloy with excellent formability at room temperature,the effect of Y,Ce,and Gd addition on texture and stretch formability of Mg–1.5Zn alloys was carried out.The result shows that Y,Ce,and Gd addition in Mg–1.5Zn alloys can effectively weaken and modify the basal plane texture,characterized by TD-split texture in which the position of basal is titled from normal direction(ND)toward transverse direction(TD).When Mg–1.5Zn alloy with Gd addition appears low texture intensity and TD-split texture,where the position of basal poles is tilted by about±35°from ND toward to TD,the largest Erichsen value of 7.0 and the elongation rate reaches 29.1%in TD direction.However,Y and Ce addition in Mg–1.5Zn alloys promote a large number of second phase particles,which cancel the contribution of the unique basal texture to stretch formability and ductility. | Zheng-Xu Cai Hai-Tao Jiang Di Tang Zhao Ma Qiang Kang | 2013 | Rare Metals2013，32，5： | 6 |
| 6 | Uncertainty-optimized deep learning model for small-scale person re-identification显示文摘In recent years, deep learning has developed rapidly and is widely used in various fields, such as computer vision, speech recognition, and natural language processing. For end-to-end person re-identification,most deep learning methods rely on large-scale datasets. Relatively few methods work with small-scale datasets. Insufficient training samples will affect neural network accuracy significantly. This problem limits the practical application of person re-identification. For small-scale person re-identification, the uncertainty of person representation and the overfitting problem associated with deep learning remain to be solved.Quantifying the uncertainty is difficult owing to complex network structures and the large number of hyperparameters. In this study, we consider the uncertainty of pedestrian representation for small-scale person re-identification. To reduce the impact of uncertain person representations, we transform parameters into distributions and conduct multiple sampling by using multilevel dropout in a testing process. We design an improved Monte Carlo strategy that considers both the average distance and shortest distance for matching and ranking. When compared with state-of-the-art methods, the proposed method significantly improve accuracy on two small-scale person re-identification datasets and is robust on four large-scale datasets. | Cairong ZHAO Kang CHEN Di ZANG Zhaoxiang ZHANG Wangmeng ZUO Duoqian MIAO | 2019 | Science China(Information Sciences)2019，62，12： | 6 |
| 7 | Medical Image Segmentation using PCNN based on Multi-feature Grey Wolf Optimizer Bionic Algorithm显示文摘Medical image segmentation is a challenging task especially in multimodality medical image analysis.In this paper,an improved pulse coupled neural network based on multiple hybrid features grey wolf optimizer(MFGWO-PCNN)is proposed for multimodality medical image segmentation.Specifically,a two-stage medical image segmentation method based on bionic algorithm is presented,including image fusion and image segmentation.The image fusion stage fuses rich information from different modalities by utilizing a multimodality medical image fusion model based on maximum energy region.In the stage of image segmentation,an improved PCNN model based on MFGWO is proposed,which can adaptively set the parameters of PCNN according to the features of the image.Two modalities of FLAIR and TIC brain MRIs are applied to verify the effectiveness of the proposed MFGWO-PCNN algorithm.The experimental results demonstrate that the proposed method outperforms the other seven algorithms in subjective vision and objective evaluation indicators. | Xue Wang Zhanshan Li Heng Kang Yongping Huang Di Gai | 2021 | Journal of Bionic Engineering2021，18，3： | 4 |
| 8 | Surface Temperature Change of U75V 60 kg/m Heavy Rail During Heat Treatment显示文摘The U75V 60 kg/m heavy rail samples were heated to 900 ℃ in a resistance furnace for a fixed duration of 50 min. Under this condition, the samples were austenitized totally. Then, the samples were dragged out of furnace and cooled for 25 s in the open air. After that, the samples entered into the air spraying channel, and were cooled from the top and both sides by compressed air. During this period, main technical parameters were changed such as the distance between spray nozzles and surface of rail head, air pressure, air spraying time and air temperature. So under laboratory condition, optimal heat-treating parameters of U75V 60 kg/m heavy rail were determined as the distance between spray nozzles and surface of rail head of 15 mm, air pressure of 0.26 MPa, cooling time of 80 s, and air temperature of 28 ℃. The surface temperature at different positions of heavy rail was measured before and after heat treatment, and the temperature changing law was determined. The self tempering occurred on the surface of rail head after heat treatment, and the tempering temperature became the largest (about 3 min) after heat treatment, separately 528, 524 and 536 ℃ at the center, top fillet and bottom fillet of rail head. The heavy rail was cooled in open air after heat treatment; during this period, the temperature gap on the surface of heavy rail became smaller and smaller, and was reduced to zero when being cooled for 20 min. | KANG Hao WU Di ZHAO Xian-ming | 2013 | Journal of Iron and Steel Research(International)2013，20，2： | 3 |
| 9 | Effect of competition on spatial patterns of oak forests on the Chinese Loess Plateau显示文摘Reforestation or natural forest regeneration is an alternative measure for controlling soil erosion in degraded land on the Chinese Loess Plateau(CLP). However, our understanding of the temporal dynamics and the spatial patterns of forest regeneration remains inadequate. Two oak forests at different development stages were investigated to determine the spatial patterns of competitions(intraspecies and interspecies) during different successional stages. The intraspecies and interspecies spatial relationships among different tree diameters at breast height were analyzed at multiple scales by Kriging interpolation method and univariate and bivariate O-ring statistics. Our analytical results indicated that self-correlation and competition intensity were relatively high between oak and pine trees in the early development stage of oak forests due to their clumped distributions of heavy seeds. Birch trees had a lower competition in comparison to oak trees although birch was the dominant species. Therefore, asymmetric competition of oak trees was most likely to have led to their edge dispersal and their success in replacing the pioneer species. Asymmetric competition means that larger individuals obtained a disproportionately large share of the resources and suppressed the growth of smaller individuals. Kriging interpolation analysis showed a tendency towards homogenization caused by interspecies competition during the succession of oak forests. Our results demonstrated that the competition was the driving factor in the spatial distribution of oak forests on the CLP. | KANG Di DENG Jian QIN Xiaowei HAO Fei GUO Shujuan HAN Xinhui YANG Gaihe | 2017 | Journal of Arid Land2017，9，1： | 3 |
| 10 | Review on structural fatigue of NiTi shape memory alloys:Pure mechanical and thermo-mechanical ones显示文摘Structural fatigue of Ni Ti shape memory alloys is a key issue that should be solved in order to promote their engineering applications and utilize their unique shape memory effect and super-elasticity more sufficiently. In this paper, the latest progresses made in experimental and theoretical analyses for the structural fatigue features of Ni Ti shape memory alloys are reviewed. First, macroscopic experimental observations to the pure mechanical and thermo-mechanical fatigue features of the alloys are summarized;then the state-of-arts in the mechanism analysis of fatigue rupture are addressed; further, advances in the construction of fatigue failure models are provided; finally, summary and future topics are outlined. | Guozheng Kang Di Song | 2015 | Theoretical & Applied Mechanics Letters2015，5，6： | 3 |
| 11 | RF-CLASS: A remote-sensing-based flood crop loss assessment cyber-service system for supporting crop statistics and insurance decision-making显示文摘Floods often cause significant crop loss in the United States. Timely and objective information on flood-related crop loss,such as flooded acreage and degree of crop damage,is very important for crop monitoring and risk management in agricultural and disaster-related decision-making at many concerned agencies. Currently concerned agencies mostly rely on field surveys to obtain crop loss information and compensate farmers' loss claim. Such methods are expensive,labor intensive,and time consumptive,especially for a large flood that affects a large geographic area. The results from such methods suffer from inaccuracy,subjectiveness,untimeliness,and lack of reproducibility. Recent studies have demonstrated that Earth observation(EO) data could be used in post-flood crop loss assessment for a large geographic area objectively,timely,accurately,and cost effectively. However,there is no operational decision support system,which employs such EO-based data and algorithms for operational flood-related crop decision-making. This paper describes the development of an EO-based flood crop loss assessment cyber-service system,RF-CLASS,for supporting flood-related crop statistics and insurance decision-making. Based on the service-orientated architecture,RF-CLASS has been implemented with open interoperability specifications to facilitate the interoperability with EO data systems,particularly the National Aeronautics and Space Administration(NASA) Earth Observing System Data and Information System(EOSDIS),for automatically fetching the input data from the data systems. Validated EO algorithms have been implemented as web services in the system to operationally produce a set of flood-related products from EO data,such as flood frequency,flooded acreage,and degree of crop damage,for supporting decision-making in flood statistics and flood crop insurance policy. The system leverages recent advances in the remote sensing-based flood monitoring and assessment,the near-real-time availability of EO data,the service-oriented architecture,geospatial interoperability standards,and the standard-based geospatial web service technology. The prototypical system has automatically generated the flood crop loss products and demonstrated the feasibility of using such products to improve the agricultural decision-making. Evaluation of system by the end-user agencies indicates that significant improvement on flood-related crop decision-making has been achieved with the system. | Liping Di Eugene G. Yu Lingjun Kang Ranjay Shrestha BAI Yu-qi | 2017 | Journal of Integrative Agriculture2017，16，2： | 3 |
| 12 | Norovirus Infection and Histo-blood Group Antigens in Children Hospitalized with Diarrhea in Lulong and Chenzhou in China显示文摘Norovirus(No V)is a pathogen that commonly causes viral diarrhea in children.Studies indicate that No V recognizes human histo-blood group antigens(HBGAs)as cell attachment factors.In order to explore the correlation between of No V infection and HBGAs,a cross-sectional study was conducted in children less than five years old who were hospitalized with diarrhea in two areas of China between November 2014 and February 2015. | SUN Xiao Man YAN Xiao Feng HE Zhi Gang Li Jing Xin GUO Ni Jun XU Zi Qian JIN Miao LI Dan Di ZHOU Yong Kang XIE Guang Cheng Pang Li Li ZHANG Qing LIU Na DUAN Zhao Jun | 2016 | Biomedical and Environmental Sciences2016，29，4： | 3 |
| 13 | Safety and efficacy of meplazumab in healthy volunteers and COVID-19 patients: a randomized phase 1 and an exploratory phase 2 trial显示文摘Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Blocking CD147 via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication.Meplazumab is a humanized anti-CD147 IgG_(2) monoclonal antibody,which may effectively prevent SARS-CoV-2 infection in coronavirus disease 2019(COVID-19)patients.Here,we conducted a randomized,double-blinded,placebo-controlled phase 1 trial to evaluate the safety,tolerability,and pharmacokinetics of meplazumab in healthy subjects,and an open-labeled,concurrent controlled add-on exploratory phase 2 study to determine the efficacy in COVID-19 patients.In phase 1 study,59 subjects were enrolled and assigned to eight cohorts,and no serious treatment-emergent adverse event(TEAE)or TEAE grade≥3 was observed.The serum and peripheral blood Cmax and area under the curve showed non-linear pharmacokinetic characteristics.No obvious relation between the incidence or titer of positive anti-drug antibody and dosage was observed in each cohort.The biodistribution study indicated that meplazumab reached lung tissue and maintained>14 days stable with the lung tissue/cardiac blood-pool ratio ranging from 0.41 to 0.32.In the exploratory phase 2 study,17 COVID-19 patients were enrolled,and 11 hospitalized patients were involved as concurrent control.The meplazumab treatment significantly improved the discharged(P=0.005)and case severity(P=0.021),and reduced the time to virus negative(P=0.045)in comparison to the control group.These results show a sound safety and tolerance of meplazumab in healthy volunteers and suggest that meplazumab could accelerate the recovery of patients from COVID-19 pneumonia with a favorable safety profile. | Huijie Bian Zhao-Hui Zheng Ding Wei Aidong Wen Zheng Zhang Jian-Qi Lian Wen-Zhen Kang Chun-Qiu Hao Jing Wang Rong-Hua Xie Ke Dong Jie-Lai Xia Jin-Lin Miao Wen Kang Guoquan Li Di Zhang Mingru Zhang Xiu-Xuan Sun Likun Ding Kui Zhang Junfeng Jia Jin Ding Zhiqin Li Yanyan Jia Lin-Na Liu Zhe Zhang Zhao-Wei Gao Hong Du Na Yao Qing Wang Ke Wang Jie-Jie Geng Bin Wang Ting Guo Ruo Chen Yu-Meng Zhu Li-Juan Wang Qian He Rui-Rui Yao Ying Shi Xiang-Min Yang Jian-Sheng Zhou Yi-Nan Ma Ya-Tao Wang Xue Liang Fei Huo Zhe Wang Yang Zhang Xu Yang Ye Zhang Lu-Hua Gao Ling Wang Xiao-Chun Chen Hao Tang Shuang-Shuang Liu Qing-Yi Wang Zhi-Nan Chen Ping Zhu | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 14 | Effect of Air Pressure on Hardened Layer of U75V 60 kg/m Heavy Rail after Heat Treatment显示文摘The samples cut from U75V 60 kg/m heavy rail are heated to 900 ℃ in resistance furnace and then put into air spraying channel to be cooled for 80 s, and change air pressure from 0.16 MPa to 0.33 MPa, and observe the effect of air pressure on hardened layer. The thickness and hardness of hardened layer increases with the increase of air pressure, and the thickness is more than 24 mm at the center and top fillets of rail head, and more than 15 mm at the blow fillets of rail head when air pressure is more than 0.26 MPa. During the tempering after heat treatment, tempering temperature of rail head is more than 570 ℃ when air pressure is separately 0.16 MPa, 0.20 MPa and 0.23 MPa, which is higher than finishing temperature of pearlite transformation at the cooling rate of 3 ℃/s according to CCT curve of U75V steel. When air pressure is separately 0.26, 0.30 and 0.33 MPa, the tempering temperature is 529 ℃ lower than finishing temperature of pearlite transformation at the cooling rate of 3 ℃/s. Under this condition, pearlite transformation is finished totally, so in order to reduce air consumption and control the cost, proper air pressure for U75V 60 kg/m heavy rail heat treating should be 0.26 MPa; the cooling rate increases with the increase of air pressure, and the average cooling rate on the surface of rail head is more than 3.21 ℃/s when air pressure is more than 0.26 MPa, and the largest cooling rate occurs at the top fillets of rail head. | Hao Kang Xian-Ming Zhao Di Wu | 2013 | Journal of Harbin Institute of Technology(New Series)2013，20，1： | 2 |
| 15 | Management of choroidal neovascularization following laser photocoagulation for central serous chorioretinopathy 显示文摘 | Ha TW Ham DI Kang SW | 2002 | Korean J Ophthalmol2002，16，2： | 2 |
| 16 | A framework for mixed-use decomposition based on temporal activity signatures extracted from big geo-data显示文摘Mixed use has been extensively applied as an urban planning principle and hinders the study of single urban functions.To address this problem,it is worth decomposing the mixed use.Inspired by the concept of spectral unmixing in remote sensing applications,this paper proposes a framework for mixed-use decomposition based on big geo-data.Mixeduse decomposition in terms of human activities differs from traditional land use research,and it is more reasonable to infer the actual urban function of land.The framework consists of four steps,namely temporal activity signature extraction,urban function base curve extraction,mixeduse decomposition,and result validation.First,the temporal activity signatures(TASs)of each zone are extracted as the proxy of human activity patterns.Second,the diurnal TASs of routine activities are extracted as urban function base curves(i.e.endmembers).Third,a linear decomposition model is used to decompose the mixed use and obtain multiple results(urban function composition,dynamic activity proportions,and the mixing index).Finally,result validation strategies are concluded.This framework offers method extensibility and has few requirements for the input data.It is validated by means of a case study of Beijing,based on a social media check-in dataset. | Lun Wu Ximeng Cheng Chaogui Kang Di Zhu Zhou Huang Yu Liu | 2020 | International Journal of Digital Earth2020，13，6： | 2 |
| 17 | High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells显示文摘在导致的 pluripotent 干细胞(iPSCs ) 积累的反常 epigenetic 修正是否能最终影响 iPSC pluripotency,仍然保持争论。为了探查这个问题,有一样的基因背景的 iPSC 线和 proviral 集成地点,被建立,并且每根 iPSC 线的 pluripotency 状态用 tetraploid (4N ) 被描绘互补试金。随后,基因表示和 “ 的全球 epigenetic 修正; 4N-ON ”并且相应 “ 4N-OFF ”iPSC 线通过深定序 mRNA 表示,小 RNA 侧面, histone 修正(H3K27me3, H3K4me3,和 H3K4me2 ) ,和 DNA methylation 的分析被比较。我们发现了印的基因的那 methylation, Zrsr1,一致地与减少的 pluripotency 在 iPSC 线被破坏。而且,破坏 methylation 不能被改进文化条件或 iPSCs 的 subcloning 救。而且,在 iPSCs 的 Zrsr1 和 pluripotency 状态的 hypomethylation 之间的关系进一步在线从另外的 reprogramming 系统导出的独立 iPSC 被验证。 | Gang Chang Shuai Gao Xinfeng Hou Zijian Xu Yanfeng Liu Lan Kang Yu Tao Wenqiang Liu Bo Huang Xiaochen Kou Jiayu Chen Lei An Kai Miao Keqian Di Zhilong Wang Kun Tan Tao Cheng Tao Cai Shaorong Gao Jianhui Tian | 2014 | Cell Research2014，24，3： | 2 |
| 18 | S100 calcium binding protein A6 and associated long noncoding ribonucleic acids as biomarkers in the diagnosis and staging of primary biliary cholangitis显示文摘BACKGROUND Primary biliary cholangitis(PBC)is a chronic and slowly progressing cholestatic disease,which causes damage to the small intrahepatic bile duct by immunoregulation,and may lead to cholestasis,liver fibrosis,cirrhosis and,eventually,liver failure.AIM To explore the potential diagnosis and staging value of plasma S100 calcium binding protein A6(S100A6)messenger ribonucleic acid(mRNA),LINC00312,LINC00472,and LINC01257 in primary biliary cholangitis.METHODS A total of 145 PBC patients and 110 healthy controls(HCs)were enrolled.Among them,80 PBC patients and 60 HCs were used as the training set,and 65 PBC patients and 50 HCs were used as the validation set.The relative expression levels of plasma S100A6 mRNA,long noncoding ribonucleic acids LINC00312,LINC00472 and LINC01257 were analyzed using quantitative reverse transcription-polymerase chain reaction.The bile duct ligation(BDL)mouse model was used to simulate PBC.Then double immunofluorescence was conducted to verify the overexpression of S100A6 protein in intrahepatic bile duct cells of BDL mice.Human intrahepatic biliary epithelial cells were treated with glycochenodeoxycholate to simulate the cholestatic environment of intrahepatic biliary epithelial cells in PBC.RESULTS The expression of S100A6 protein in intrahepatic bile duct cells was up-regulated in the BDL mouse model compared with sham mice.The relative expression levels of plasma S100A6 mRNA,log10 LINC00472 and LINC01257 were upregulated while LINC00312 was down-regulated in plasma of PBC patients compared with HCs(3.01±1.04 vs 2.09±0.87,P<0.0001;2.46±1.03 vs 1.77±0.84,P<0.0001;3.49±1.64 vs 2.37±0.96,P<0.0001;1.70±0.33 vs 2.07±0.53,P<0.0001,respectively).The relative expression levels of S100A6 mRNA,LINC00472 and LINC01257 were up-regulated and LINC00312 was down-regulated in human intrahepatic biliary epithelial cells treated with glycochenodeoxycholate compared with control(2.97±0.43 vs 1.09±0.08,P=0.0018;2.70±0.26 vs 1.10±0.10,P=0.0006;2.23±0.21 vs 1.10±0.10,P=0.0011;1.20±0.04 vs 3.03±0.15,P<0.0001,respectively).The mean expression of S100A6 in the advanced stage(III and IV)of PBC was up-regulated compared to that in HCs and the early stage(II)(3.38±0.71 vs 2.09±0.87,P<0.0001;3.38±0.71 vs 2.57±1.21,P=0.0003,respectively);and in the early stage(II),it was higher than that in HCs(2.57±1.21 vs 2.09±0.87,P=0.03).The mean expression of LINC00312 in the advanced stage was lower than that in the early stage and HCs(1.39±0.29 vs 1.56±0.33,P=0.01;1.39±0.29 vs 2.07±0.53,P<0.0001,respectively);in addition,the mean expression of LINC00312 in the early stage was lower than that in HCs(1.56±0.33 vs 2.07±0.53,P<0.0001).The mean expression of log10 LINC00472 in the advanced stage was higher than those in the early stage and HCs(2.99±0.87 vs 1.81±0.83,P<0.0001;2.99±0.87 vs 1.77±0.84,P<0.0001,respectively).The mean expression of LINC01257 in both the early stage and advanced stage were up-regulated compared with HCs(3.88±1.55 vs 2.37±0.96,P<0.0001;3.57±1.79 vs 2.37±0.96,P<0.0001,respectively).The areas under the curves(AUC)for S100A6,LINC00312,log10 LINC00472 and LINC01257 in PBC diagnosis were 0.759,0.7292,0.6942 and 0.7158,respectively.Furthermore,the AUC for these four genes in PBC staging were 0.666,0.661,0.839 and 0.5549,respectively.The expression levels of S100A6 mRNA,log10 LINC00472,and LINC01257 in plasma of PBC patients were decreased(2.35±1.02 vs 3.06±1.04,P=0.0018;1.99±0.83 vs 2.33±0.96,P=0.036;2.84±0.92 vs 3.69±1.54,P=0.0006),and the expression level of LINC00312 was increased(1.95±0.35 vs 1.73±0.32,P=0.0007)after treatment compared with before treatment using the paired t-test.Relative expression of S100A6 mRNA was positively correlated with log10 LINC00472(r=0.683,P<0.0001);serum level of collagen type IV was positively correlated with the relative expression of log10 LINC00472(r=0.482,P<0.0001);relative expression of S100A6 mRNA was positively correlated with the serum level of collagen type IV(r=0.732,P<0.0001).The AUC for the four biomarkers obtained in the validation set were close to the training set.CONCLUSION These four genes may potentially act as novel biomarkers for the diagnosis of PBC.Moreover,LINC00472 acts as a potential biomarker for staging in PBC. | Xi-Hua Dong Di Dai Zhi-Dong Yang Xiao-Ou Yu Hua Li Hui Kang | 2021 | World Journal of Gastroenterology2021，27，17： | 2 |
| 19 | ISG20 inhibits bluetongue virus replication显示文摘ISG20 is an interferon-inducible exonuclease that inhibits virus replication.Although ISG20 is thought to degrade viral RNA,the antiviral mechanism and specificity of ISG20 remain unclear.In this study,the antiviral role of ovine ISG20(o ISG20)in bluetongue virus(BTV)infection was investigated.It was found that BTV infection upregulated the transcription of ovine ISG20(o ISG20)in a time-and BTV multiplicity of infection(MOI)-dependent manner.Overexpression of o ISG20 suppressed the production of BTV genome,proteins,and virus titer,whereas the knockdown of o ISG20 increased viral replication.o ISG20 was found to co-localize with BTV proteins VP4,VP5,VP6,and NS2,but only directly interacted with VP4.Exonuclease defective o ISG20 significantly decreased the inhibitory effect on BTV replication.In addition,the interaction of mutant o ISG20 and VP4 was weakened,suggesting that binding to VP4 was associated with the inhibition of BTV replication.The present data characterized the anti-BTV effect of o ISG20,and provides a novel clue for further exploring the inhibition mechanism of double-stranded RNA virus by ISG20. | Di Kang Shandian Gao Zhancheng Tian Guorui Zhang Guiquan Guan Guangyuan Liu Jianxun Luo Junzheng Du Hong Yin | 2022 | Virologica Sinica2022，37，4： | 2 |
| 20 | Techno-economic Analysis of Contingency Reserve Allocation Scheme for Combined UHV DC and AC Receiving-End Power System显示文摘In a UHV DC and AC combined system the UHVDC’s blocking fault becomes the most serious load balance contingency of the receiving end power system due to its large capacity.The amount of contingency reserve thus is largely increased.This paper proposes three distinct schemes for allocating contingency reserve to cover the power shortage caused by UHVDC’s blocking fault,and compares the economic cost of these schemes.An operation simulation method using a unit commitment technique is proposed for evaluating the operation costs associated with increasing contingency reserve.A real case study for a Jiangsu Province power system in China is presented to demonstrate that the best scheme is to share the contingency reserve with neighborhood power systems through the transmission capacity of UHVAC lines. | Di Wu Ning Zhang Chongqing Kang Yi Ge Zhenjian Xie Junhui Huang | 2016 | CSEE Journal of Power and Energy Systems2016，2，2： | 2 |
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