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| 1 | Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity显示文摘AIM To establish a model to enrich and characterize stemlike cells from murine normal liver and hepatocellular carcinoma(HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition(EMT).METHODS In this study,we utilized a stem cell conditioned serumfree medium to enrich stem-like cells from mouse HCC and normal liver cell lines,Hepa 1-6 and AML12,respectively.We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating theRNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR(q RTPCR).Next,we examined the relationship between stem cells and EMT using q RT-PCR.RESULTS Three-dimensional spheres were enriched by culturing murine HCC and normal hepatocyte cell lines in stem cell conditioned serum-free medium supplemented with epidermal growth factor,basic fibroblast growth factor and heparin sulfate.The 3-dimensional spheres had enhanced stemness markers such as Klf4 and Bmi1 and hepatic cancer stem cell(CSC) marker Cd44 compared to parental cells grown as adherent cultures.We report that epithelial markers E-cadherin and ZO-1 were downregulated,while mesenchymal markers Vimentin and Fibronectin were upregulated in 3-dimensional spheres.The 3-dimensional spheres also exhibited changes in expression of Snai,Zeb and Twist family of EMT transcription factors.CONCLUSION Our novel method successfully enriched stem-like cells which possessed an EMT phenotype.The isolation and characterization of murine hepatic CSCs could establish a precise target for the development of more effective therapies for HCC. | Aparna Jayachandran Ritu Shrestha Bijay Dhungel I-Tao Huang Marianna Yumi Kawashima Vasconcelos Brian J Morrison Charmaine A Ramlogan-Steel Jason C Steel | 2017 | World Journal of Stem Cells2017，9，9： | 4 |
| 2 | Imaging of nontraumatic hemorrhage of the adrenal gland显示文摘 | Kawashima A Sandler C M Ernst R D | 1999 | Radiographics1999，19，： | 1 |
| 3 | Adaptation of HIV-1to human leukocyte antigen class I显示文摘 | Kawashima Y Pfafferott K Frater J Matthews P Payne R Addo M Gatanaga H Fujiwara M Hachiya A Koizumi H Kuse N Oka S Duda A Prendergast A Crawford H Leslie A Brumme Z Brumme C Allen T Brander C Kaslow R Tang J Hunter E Allen S Mulenga J Branch S Roach T John M Mallal S Ogwu A Shapiro R Prado J G Fidler S Weber J Pybus O G Klenerman P Ndung'u T Phillips R Heckerman D Harrigan P R Walker B D Takiguchi M Goulder P | 2009 | Nature2009，458，7238： | 1 |
| 4 | 3489 hourly thermal load prediction for the next 24 hours by ARIMA, EWMA, LR, and an artificialneural network显示文摘 | Kawashima M Dorgan C E Mitchell J W | 1995 | ASHRAE Trans1995，101，1： | 1 |
| 5 | Relationship between metabolic hormones and ovulation of dominant follicle during the first follicular wave post-partum in high-producing dairy cows显示文摘 | Kawashima C Fukihara S Maeda M | | 0，，： | 1 |
| 6 | CT of renal inflammatory disease显示文摘 | Kawashima A Sandle C M Goldman S M | 1997 | Radio Graphics1997，17，： | 1 |
| 7 | Imaging of renal trauma: a comprehensive review 显示文摘 | KAWASHIMA A SANDLER C M CORL F M et at | 2001 | Radiographics2001，21，3： | 1 |
| 8 | Glass fiber reinforced flexible composite material using soft fluorofesin显示文摘 | KATSURAGAWA S KAWASHIMA C MASAKI T | 1998 | Ausz Eur Patentanmeld I1998，26，3： | 1 |
| 9 | Hourly thermal load prediction for the next 24 hours by ARIMA,EWA,LR,and an artificial neural network显示文摘 | Kawashima M C E Dotgan J W Mitchell | 1995 | ASHRAE Transactions1995，101，： | 1 |
| 10 | Complete genomic sequence of the genome of the filamentous nitrogen-fixing cyanobacerium Anabaena sp.strain PCC 7120显示文摘 | Kaneko T Nakamura Y Wolk C P Kuritz T, Sasamoto S, Watanabe A, Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M, Takazawa M, Yamada M, Yasuda M, Tabata S | | 0，，： | 1 |
| 11 | Improtance of the carboxy-terminus of human interleukin-11 in conserving its biological activity显示文摘 | Ohshimura C Kawashima I | 1996 | Biosci Biotechnol Biochem1996，60，3： | 1 |
| 12 | Inguinal hernia repair in children using single-incision laparoscopic-assisted percutaneous extra peritoneal closure 显示文摘 | Uchida H Kawashima H Goto C | 2010 | J Pediatr Surg2010，45，12： | 1 |
| 13 | lnguinal hernia repair in children using single-incision laparoscopfc-assisted percutaneous extraper-itoneal closure显示文摘 | Uchida H Kawashima H Goto C | 2010 | J Pediatr Surg2010，45，7： | 1 |
| 14 | Stereotic CT scan applied to stereotactic thalamotomy and biopsy 显示文摘 | Ohye C Kawashima Y Hirato M | 1984 | Acta Neurochirurgica1984，71，： | 1 |
| 15 | Inguinal hernia repair in children using single - incision laparoscopie - as- sisted percutaneous extraperitoneal closure 显示文摘 | Uehida H Kawashima H Goto C | 2010 | J Pediatr Surg2010，45，12： | 1 |
| 16 | Inguinal hernia repair in children using single-incision laparoscopic-assisted percu-taneous extraperitoneal closure 显示文摘 | Uchida H Kawashima H Goto C | 2010 | J Pediatr Surg2010，45，12： | 1 |
| 17 | NOx (nitrite/ nitrate) in patients with pediatric nephrotic syndrome 显示文摘 | Kawashima H Kashiwagi Y Watanabe C | 2007 | Pediatr Nephrol2007，22，6： | 1 |
| 18 | Transaminase in rota- virus gastroentedtis 显示文摘 | Kawashima H Ishii C Ioi H | 2012 | Pediatr Int2012，54，1： | 1 |
| 19 | Hourly thermal load prediction for the next 24 hours by ARIMA, EWMA,LR, and an artificial neural network 显示文摘 | M Kawashima C E Dorgan J W Mitchell | 1995 | ASHRAE Tram1995，101，1： | 1 |
| 20 | Hourly thermal load prediction for the next 24 hours by ARIMA,EWA,LR,and an artifical neural network 显示文摘 | Kawashima M Dorgan C E Mitchell J W | 1995 | ASHRAE Trans1995，101，1： | 1 |
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