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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Tight junction disruption: Helicobacter pylori and dysregulation of the gastric mucosal barrier显示文摘Long-term chronic infection with Helicobacter pylori(H. pylori) is a risk factor for gastric cancer development. In the multi-step process that leads to gastric cancer,tight junction dysfunction is thought to occur and serve as a risk factor by permitting the permeation of luminal contents across an otherwise tight mucosa. Mechanisms that regulate tight junction function and structure in the normal stomach,or dysfunction in the infected stomach,however,are largely unknown. Although conventional tight junction components are expressed in gastric epithelial cells,claudins regulate paracellular permeability and are likely the target of inflammation or H. pylori itself. There are 27 different claudin molecules,each with unique properties that render the mucosa an intact barrier that is permselective in a way that is consistent with cell physiology. Understanding the architecture of tight junctions in the normal stomach and then changes that occur during infection is important but challenging,because most of the reports that catalog claudin expression in gastric cancer pathogenesis are contradictory. Furthermore,the role of H. pylori virulence factors,such as cytotoxin-associated gene A and vacoulating cytotoxin,in regulating tight junction dysfunction during infection is inconsistent in different gastric cell lines and in vivo,likely because non-gastric epithelial cell cultures were initially used to unravel the details of their effects on the stomach. Hampering further study,as well,is the relative lack of cultured cell models that have tight junction claudins that are consistent with native tissues. This summary will review the current state of knowledge about gastric tight junctions,normally and in H. pylori infection,and make predictions about the consequences of claudin reorganization during H. pylori infection. | Tyler J Caron Kathleen E Scott James G Fox Susan J Hagen | 2015 | World Journal of Gastroenterology2015，21，40： | 7 |
| 3 | Clinical Practice Guidelines for the Management of Pain, Agitation, and Delirium in Adult Patients in the Intensive Care Unit显示文摘 | Juliana Barr Gilles L. Fraser Kathleen Puntillo E. Wesley Ely Céline Gélinas Joseph F. Dasta Judy E. Davidson John W. Devlin John P. Kress Aaron M. Joffe Douglas B. Coursin Daniel L. Herr Avery Tung Bryce R. H. Robinson Dorrie K. Fontaine Michael A. Ramsa | 2013 | Critical Care Medicine2013，，1： | 6 |
| 4 | The Effects of Pedicle Screw Fit: An In Vitro Study显示文摘 | Anna G U Brantley Jack K. Mayfield James B. Koeneman Kathleen R. Clark | 1994 | Spine1994，，15： | 2 |
| 5 | Clinical Practice Guidelines for the Management of Pain, Agitation, and Delirium in Adult Patients in the Intensive Care Unit显示文摘 | Juliana Barr Gilles L. Fraser Kathleen Puntillo E. Wesley Ely Céline Gélinas Joseph F. Dasta Judy E. Davidson John W. Devlin John P. Kress Aaron M. Joffe Douglas B. Coursin Daniel L. Herr Avery Tung Bryce R. H. Robinson Dorrie K. Fontaine Michael A. Ramsa | 2013 | Critical Care Medicine2013，，1： | 2 |
| 6 | Human acute myelogenous leukemia stem cells are rare and heterogeneous when assayed in NOD/SCID/IL2R[gamma]c-deficient mice显示文摘 | Sarry Jean-Emmanuel Murphy Kathleen Perry Robin Sanchez Patricia V Secreto Anthony Keefer Cathy Swider Cezary R Strzelecki Anne-Claire Cavelier Cindy Récher Christian Mansat-De Mas Véronique Delabesse Eric Danet-Desnoyers G Carroll Martin | 2011 | Journal of Clinical Investigation2011，，1： | 2 |
| 7 | Cardiac amyloidoma with IgM-κ gammopathy显示文摘 | Kathleen JW Gerald G Guillermo AH | 1994 | Arch Pathol Lab Med1994，118，： | 1 |
| 8 | Theoretical and experimental considerations for interpretationof amide I bands in tissue 显示文摘 | KATHLEEN M G LSAN T MARZENA Z K | 2011 | Vibrational Spectroscopy2011，53，1： | 1 |
| 9 | Effects of retinoic acid on airspace development and lung collagen in hyperoxia-exposed newborn rats显示文摘 | Kathleen A Meehan V Frank G | 2000 | Pediatr Res2000，48，： | 1 |
| 10 | EQ-5D visual analog scale andutility index values in individuals with diabetes and atrisk for diabetes:Findings from the Study to Help Im-prove Early evaluation and management of risk factorsLeading to Diabetes(SHIELD) 显示文摘 | Susan G Kathleen M F | 2008 | Health and Quality ofLife Outcomes2008，6，： | 1 |
| 11 | ChemBank: asmall-molecule screening and cheminformatics resource database显示文摘 | Kathleen P S Gregory A G Mary P H | 2008 | Nucleic Acids Res2008，36，1： | 1 |
| 12 | Mannose- binding protein genetic polymorphisms in black patients with systemic lupus erythematosus显示文摘 | Kathleen S Candra W DanIEL G | 1996 | Arthritis Rheum1996，39，12： | 1 |
| 13 | The effects of depleted self-control strength on skill-based task performance显示文摘 | DESMOND M KATHLEEN A MARTIN G et a l | 2013 | J Sport Exe Psychol2013，35，： | 1 |
| 14 | Expression of Distinct Self-Incompatibility Specificities in Arabidopsis thaliana显示文摘 | Boggs Nathan A Dwyer Kathleen G Shah Paurush McCulloch Amanda A Bechsgaard Jesper Schierup Mikkel H Nasrallah Mikhail E Nasrallah June B | 2009 | Genetics2009，，4： | 1 |
| 15 | 17 β-estradiol acts directly on the clonal osteoblastic cell line UMR 106显示文摘 | GRAY T K TIMOTHY C F KATHLEEN M G | 1987 | Proc Natl Acad Sci1987，84，17： | 1 |
| 16 | Effects of retinoic acid on airspace development and lung collagen in hyperoxia-exposed newborn rats显示文摘 | Kathleen A Veness-Meehan Frank G | 2000 | Pediatr Res2000，48，： | 1 |
| 17 | Determina-tion of chondroitin sulfate in nutritional supplements by liguid chromatography 显示文摘 | Wayne K W Kathleen G Andrew G B | 2000 | Journal of liguid chromatography and relation technology2000，23，18： | 1 |
| 18 | Evolution and stratigraphic architecture of marine slope gully complexes: Monterey Formation (Miocene), Gaviota Beach, California 显示文摘 | Kathleen D S Richard B W Stephan A G | 2009 | Marine and Petroleum Geology2009，26，2： | 1 |
| 19 | Engineering of herbicide-resistant alfalfa and evaluation under field conditions显示文摘 | Kathleen D H Johan B Willy D G | 1990 | Crop Science1990，，30： | 1 |
| 20 | Effect of Coagulant Type and Storage Temperature on the Functionality of Reduced-Fat Mozzarella Cheese显示文摘 | JEREMIAH J S KATHLEEN O S TIMOTHY P G | 2004 | EDP Sciences2004，84，： | 1 |
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