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| 1 | Role of HSP-90 for increased nNOS-mediated vasodilation in mesenteric arteries in portal hypertension显示文摘AIM:To explore the role of heat shock protein-90 (HSP-90) for nitrergic vasorelaxation in the splanchnic circulation in rats with and without portal hypertension. METHODS: Neuronal nitric oxide synthase (nNOS) and HSP-90 were analyzed by immunofluorescence, western blotting and co-immunoprecipitation in the mesenteric vasculature and isolated nerves of portal-vein-ligated (PVL) rats and sham operated rats. In vitro perfused de-endothelialized mesenteric arterial vasculature was preconstricted with norepinephrine (EC80) and tested for nNOS-mediated vasorelaxation by periarterial nerve stimulation (PNS, 2-12 Hz, 45V) before and after incubation with geldanamycin (specific inhibitor of HSP-90 signalling, 3 μg/mL) or L-NAME (non-specific NOSblocker, 10-4 mol/L). RESULTS: nNOS and HSP-90 expression was significantly increased in mesenteric nerves from PVL as compared to sham rats. Moreover, nNOS and HSP-90 were visualized in mesenteric nerves by immunofluorescence and immunoprecipitation of nNOS co-immunoprecitated HSP-90 in sham and PVL rats. PNS induced a frequencydependent vasorelaxation which was more pronounced in PVL as compared to sham rats. L-NAME and geldanamycin markedly reduced nNOS-mediated vasorelaxation abrogating differences between the study groups. The effect of L-NAME and geldanamycin on nNOS-mediated vasorelaxation was significantly greater in PVL than in sham animals. However, no difference in magnitude of effect between L-NAME and geldanamycin was noted. CONCLUSION: HSP-90 acts as a signalling mediator of nNOS-dependent nerve mediated vascular responses in mesenteric arteries, and the increased nitrergic vasorelaxation observed in portal hypertension is mediated largely by HSP-90. | Lukas Moleda Lars Jurzik Matthias Froh Erwin Gbele Claus Hellerbrand Rainer H Straub Jürgen Schlmerich Reiner Wiest | 2010 | World Journal of Gastroenterology2010，16，15： | 4 |
| 2 | Evaluation of pepper mild mottle virus, human picobirnavirus and Torque teno virus as indicators of fecal contamination in river water显示文摘 | Ibrahim Ahmed Hamza Lars Jurzik Klaus überla Michael Wilhelm | 2010 | Water Research2010，，3： | 1 |
| 3 | Methods to detect infectious human enteric viruses in environmental water samples显示文摘 | Hamza I A Jurzik L Uberla K | 2011 | Int J Hyg Environ Health2011，214，： | 1 |
| 4 | Colonization of patients and contamination of the patients’ environment by MRSA under conditions of single-room isolation显示文摘 | Ute Rohr Andrzej Kaminski Michael Wilhelm Lars Jurzik S?ren Gatermann Gert Muhr | 2008 | International Journal of Hygiene and Environmental Health2008，，2： | 1 |
| 5 | Up-regulation of Nnos and associated increase in nitrergic vasodilation in superior mesenteric arteries in pre-hapatic portal hypertention显示文摘 | Jurzik L Fron M Straub RH | 2005 | J Hepatol2005，43，2： | 1 |
| 6 | Evaluation of pepper mild mottle virus, human pieobirnavirus and Torque teno virus as indicators of fecal contamination in river water 显示文摘 | HAMZA IA JURZIK L UBERLA K | 2011 | Water Res2011，45，3： | 1 |
| 7 | Detection and quantification of human bocavirus in river water 显示文摘 | HAMZA I A JURZIK L WILHELM M | 2009 | J Gen Virol2009，90，11： | 1 |
| 8 | Methods to detect infectious human enteric viruses in environmental water samples 显示文摘 | Hamza IA Jurzik L Uberla K | 2011 | Int J Hyg Environ Health2011，214，： | 1 |
| 9 | Colonization of patients and contamination of the patients' environment by MRSA under conditions of single-room isolation显示文摘 | Rohr U Kaminski A Wilhelm M Jurzik L Gatermann S Muhr G | | International Journal of Hygiene and Environmental Health0，，： | 1 |
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