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| 1 | In vivo and in vitro phytochemical and antibacterial efficacy of Baliospermum montanum (Willd.) Muell.Arg.显示文摘Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in vitro derived rootlets and leaves segments of B.montanum were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-D either alone or in combinations.The preliminary phytochemical screening was performed by Harborne method.Antibacterial efficacy was performed by well diffusion method and incubated for 24 h at 37℃.Results:The highest percentage of callus formation(leaves segments 86.9±0.56:root segments 78.7±0.51) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 2.0 mg/L of 2.4-Dichlorophenoxy acetic acid.The phytochemical study revealed the high quantity presence of steroids,triterpenoids,glycosides,saponins,alkaloids,flavanoids, phenolic compounds,tannins,sugars etc of root and leaves derived calli.The ethanol extract of leaves segment derived calli of B.montanum showed the maximum solubility and antimicrobial activity with the MIC ranged from 100 to 200μL.Conclusions:The preliminary phytochemical study confirmed that the calli mediated tissues showed the higher percentage of metabolite constituents and extraction value compared to the in vivo leaves and roots.The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds. | Johnson M Wesely EG Zahir Hussain MI Selvan N | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，11： | 6 |
| 2 | Distribution and phosphorylation of the growth-associated protein GAP-43 in regenerating sympathetic neurons in culture显示文摘 | Meiri KF Willard M Johnson MI | | 0，，07： | 1 |
| 3 | Electrical acustimulation of the wrist for chronic neck pain: a randomized, sham- controlled trial using a wrist - ankle acustimulation device显示文摘 | Chan DK Johnson MI Sun KO | 2009 | Clin J Pain2009，25，4： | 1 |
| 4 | Acupuncture for cancer-induced bone pain?显示文摘 | Paley CA Bennett MI Johnson MI | 2011 | Evid Based Complement Alternat Med2011，，67： | 1 |
| 5 | Diaphragm electromyogram measured with unilateral magnetic stimulation显示文摘 | Luo YM Johnson LC Polkey MI | 1999 | Eur Respir J1999，13，2： | 1 |
| 6 | Efficacy of low-level laser therapy in the management of neck pain : a systematic review and meta-analysis of randomized placebo or active-treatment controlled tri- als 显示文摘 | Chow RT Johnson MI Lopes-Martins RA | 2009 | Lancet2009，374，9705： | 1 |
| 7 | An investigation into the hypoalgesic ef- fects of high- and low-frequency transcutaneous electrical nerve stimulation (tens) on experimentally-induced blunt pressure pain in healthy hunmn participants 显示文摘 | Chen CC Johnson MI | 2010 | J Pain2010，11，1： | 1 |
| 8 | Ultrasound screening for fetal chromosome anomalies显示文摘 | Drugan A Johnson ME Evans MI | 2000 | AmJ Med Genetics2000，90，11： | 1 |
| 9 | Expression of Bcl-2, Bax,and p53 in high-grade prostatic intraepithelial neoplasia and localized prostate cancer: relationship with apoptosis and proliferation 显示文摘 | Robinson MC Marsh C | 1998 | Prostate1998，37，4： | 1 |
| 10 | The low density lipoprotein receptor related protein 6 interacts with glycogen synthase kinase 3 and attenuates activity显示文摘 | Mi K Dolan PJ Johnson GV | 2006 | J Biol Chem2006，281，8： | 1 |
| 11 | Acupuncture for cancer pain in adults显示文摘 | Paley CA Johnson MI Tashani OA | 2015 | Cochrane Database Syst Rev2015，，： | 1 |
| 12 | Expression of Bc1-2, Bax, and p53 in high-grade prostatic intraepithelial neoplasia and localized prostate cancer: relationship with apoptosis and proliferation显示文摘 | Johnson MI Robinson MC March C | 1998 | Prostate1998，37，： | 1 |
| 13 | Acupuncture for cancer pain in adults显示文摘 | Paley CA Johnson MI Tashani OA | 2011 | Cochrane Database Syst Rev2011，19，00： | 1 |
| 14 | Integration of genetics and ultrasonography in prenatal diagnosis: Just looking is not enough显示文摘 | Evans MI Hume RF JR Johnson MP | 1966 | Am J Obstet Gynecol1966，174，6： | 1 |
| 15 | The thermal effects of therapeutic lasers with 810 nm and 904 nm wavelengths on human skin显示文摘 | Joensen J Demmink J H Johnson MI | 2011 | Photomed Laser Surg2011，29，3： | 1 |
| 16 | Genotyping of single nucleotide polymorphisms in cytokine genes using real - time PCR allelic dis- crimination technology显示文摘 | Johnson V J Yucesoy B Luster MI | 2004 | Cytokine2004，27，6： | 1 |
| 17 | The low density lipoprotein receptor-related protein 6 interacts with glycogen synthase kinase 3 and attenuates activity 显示文摘 | Mi K Dolan P J Johnson GV | 2006 | J Biol Chem2006，281，8： | 1 |
| 18 | Zoledronie acid treatment of osteopomsis : effects in men 显示文摘 | JOHNSON DA WILLIAMS MI PETKOV VI | 2010 | Endocr Pract2010，16，6： | 1 |
| 19 | The low density lipoprotein receptor-related protein 6 interacts with glycogen synthase kinase 3 and attenuates activity显示文摘 | Mi K Dolan PJ Johnson GV | 2006 | J Biol Chem2006，281，8： | 1 |
| 20 | PCNN models and application显示文摘 | Johnson J L Padget T MI | 1999 | IEEE Transactions on Neural Networks1999，10，3： | 1 |
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