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| 1 | High-throughput fat quantifications of hematoxylin-eosin stained liver histopathological images based on pixel-wise clustering显示文摘Besides diagnosis of fatty liver disease(FLD) using multiple medical imaging techniques in clinic,accurate fat quantification of liver tissue slice, especially the fat droplets measurement, is still a critical indicator in related pathological researches. Stained by hematoxylin-eosin(HE), different tissue components with different colors need to be identified and measured manually in conventional approaches. Automated liver fat quantification of HE stained images remains challenging because forms and distributions of fat are extremely irregular with no clear boundaries, especially in conducting high-throughput analysis which demands quick processing and higher accuracy for the reference of pathologists. To solve this problem, we propose an automated liver fat quantifications pipeline of HE stained images based on pixel-wise clustering, which firstly extracts high-relevant pixel-level features with color mode transformation, then locates boundaries between nuclei, fat and other components by clustering image pixels in an unsupervised mode, and finally identifies indicative fat droplets based on a set of morphological criteria. The pipeline was verified in analysis of multifold fatty liver treatment assays, with experimental results showing high accuracy and adaptability in fat droplets quantification despite data variance. Quantitative indicators provide a reliable evidence for relevant pathological researches or therapy selection, in which number and average area of indicative fat droplets increased sharply in severe and moderate-grade FLD respectively. Those indicators might be utilized as surrogate biomarkers for further researches. | Peng SHI Junru CHEN Jinke LIN Lurong ZHANG | 2017 | Science China(Information Sciences)2017，60，9： | 1 |
| 2 | The grain potential of cultivated lands in Mainland China in 2004显示文摘 | Jinke Zhang Fengrong Zhang Di Zhang Dexian He Lin Zhang Chuguo Wu Xiangbin Kong | 2008 | Land Use Policy2008，，1： | 1 |
| 3 | 5mC DNA methylation modification-mediated regulation in tissue functional differentiation and important flavor substance synthesis of tea plant (Camellia sinensis L.)显示文摘In plants,5mC DNA methylation is an important and conserved epistatic mark involving genomic stability,gene transcriptional regulation,developmental regulation,abiotic stress response,metabolite synthesis,etc.However,the roles of 5mC DNA methylation modification(5mC methylation)in tea plant growth and development(in pre-harvest processing)and flavor substance synthesis in pre-and post-harvest processing are unknown.We therefore conducted a comprehensive methylation analysis of four key pre-harvest tissues(root,leaf,flower,and fruit)and two processed leaves during oolong tea post-harvest processing.We found that differential 5mC methylation among four key tissues is closely related to tissue functional differentiation and that genes expressed tissue-specifically,responsible for tissue-specific functions,maintain relatively low 5mC methylation levels relative to non-tissue-specifically expressed genes.Importantly,hypomethylation modifications of CsAlaDC and TS/GS genes in roots provided the molecular basis for the dominant synthesis of theanine in roots.In addition,integration of 5mC DNA methylationomics,metabolomics,and transcriptomics of post-harvest leaves revealed that content changes in flavor metabolites during oolong tea processing were closely associated with transcription level changes in corresponding metabolite synthesis genes,and changes in transcript levels of these important synthesis genes were strictly regulated by 5mC methylation.We further report that some key genes during processing are regulated by 5mC methylation,which can effectively explain the content changes of important aroma metabolites,includingα-farnesene,nerolidol,lipids,and taste substances such as catechins.Our results not only highlight the key roles of 5mC methylation in important flavor substance synthesis in pre-and post-harvest processing,but also provide epimutation-related gene targets for future improvement of tea quality or breeding of whole-tissue high-theanine varieties. | Weilong Kong Qiufang Zhu Qing Zhang Yiwang Zhu Jingjing Yang Kun Chai Wenlong Lei Mengwei Jiang Shengcheng Zhang Jinke Lin Xingtan Zhang | 2023 | Horticulture Research2023，10，8： | 0 |
| 4 | The development and characterization ot recombinant adenovirus encoding RA538, p53 or p16 for gene therapy of cancer显示文摘We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficiency assay showed thatthe infected cancer cells inoculated with theserecombinant adenoviruses at MOI of 25 or greaterexhibited 100% transduction efficiency. The growthrates and colony formation of cancer cells infectedwith these recombinant adenoviruses were | Jinke Cheng, Chen Lin, Xueyan Zhang, Rong Xing, Juwei Mu, Min WuNational Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, and Peking Union Medical College, Beijing 100021 | 1997 | 中国实验血液学杂志1997，5，3： | 0 |
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