|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Biomimetic polycaprolactone-chitosan nanofibrous substrate influenced cell cycle and ECM secretion affect cellular uptake of nanoclusters显示文摘Biomimetic cell culture substrates are developed as an alternative to the conventional substrates.They provide necessary biochemical and biophysical cues to the cells from their surrounding environment for their optimal growth,behaviour and physiology.Changes in physiology of cells growing on biomimetic substrate can essentially affect results of in vitro biological experiments such as drug cytotoxicity,nanoparticle internalization or signalling pathways.As majority of ECM proteins are fibrous in nature,nanofibrous scaffolds have more biomimicking properties.Therefore,in this study,we developed ECM mimicking polycaprolactone-chitosan nanofiber substrate and evaluated its effect on cell morphology,proliferation,cell cycle and ECM production.Further,cellular uptake of BSA-AuNCs has been assessed on conventional and biomimetic substrate in order to demonstrate the effect of these events on cellular properties.It was observed that the cells that were grown for 15 days on the nanofibers,had majority of cells in the proliferative phase of cell cycle compared to TCPS.Moreover,these cells showed extensive collagen and fibronectin production.Due to these conditions C3H10T1/2 cells displayed higher cell internalization of BSA-AuNCs.Overall,this study indicates that the nano-topographical and biochemical environment could alter the cell proliferative behaviour and ECM production,which affects the cell internalization of BSA-AuNCs.Also,PCL-chitosan nanofibrous substrate could be a better alternative to TCPS for cell culture studies. | Dhwani Jhala Hilal Rather Dhaval Kedaria Juhi Shah Sanjay Singh Rajesh Vasita | 2019 | Bioactive Materials2019，4，1： | 4 |
| 2 | Antioxidative study of Cerium Oxide nanoparticle functionalised PCL-Gelatin electrospun fibers for wound healing application显示文摘Skin wound healing involves a coordinated cellular response to achieve complete reepithelialisation.Elevated levels of reactive oxygen species(ROS)in the wound environment often pose a hindrance in wound healing resulting in impaired wound healing process.Cerium oxide nanoparticles(CeNPs)have the ability to protect the cells from oxidative damage by actively scavenging the ROS.Furthermore,matrices like nanofibers have also been explored for enhancing wound healing.In the current study CeNP functionalised polycaprolactone(PCL)-gelatin nanofiber(PGNPNF)mesh was fabricated by electrospinning and evaluated for its antioxidative potential.Wide angle XRD analysis of randomly oriented nanofibers revealed^2.6 times reduced crystallinity than pristine PCL which aided in rapid degradation of nanofibers and release of CeNP.However,bioactive composite made between nanoparticles and PCLgelatin maintained the fibrous morphology of PGNPNF upto 14 days.The PGNPNF mesh exhibited a superoxide dismutase(SOD)mimetic activity due to the incorporated CeNPs.The PGNPNF mesh enhanced proliferation of 3T3-L1 cells by^48%as confirmed by alamar blue assay and SEM micrographs of cells grown on the nanofibrous mesh.Furthermore,the PGNPNF mesh scavenged ROS,which was measured by relative DCF intensity and fluorescence microscopy;and subsequently increased the viability and proliferation of cells by three folds as it alleviated the oxidative stress.Overall,the results of this study suggest the potential of CeNP functionalised PCL-gelatin nanofibrous mesh for wound healing applications. | Hilal Ahmad Rather Ria Thakore Ragini Singh Dhwani Jhala Sanjay Singh Rajesh Vasita | 2018 | Bioactive Materials2018，3，2： | 4 |
| 3 | Layered inorganic nanocomposites: A promising carrier for 5-fluorouracil (5-FU)显示文摘 | Bhavesh D. Kevadiya Tapan A. Patel Devendrasinh D. Jhala Rahul P. Thumbar Harshad Brahmbhatt Maharshi P. Pandya Shalini Rajkumar Prasant K. Jena Ghanshyam V. Joshi Pankaj K. Gadhia C.B. Tripathi Hari C. Bajaj | 2012 | European Journal of Pharmaceutics and Biopharmaceutics2012，，： | 2 |
| 4 | Tumor-Derived Granulocyte-Macrophage Colony-Stimulating Factor Regulates Myeloid Inflammation and T Cell Immunity in Pancreatic Cancer显示文摘 | Lauren J. Bayne Gregory L. Beatty Nirag Jhala Carolyn E. Clark Andrew D. Rhim Ben Z. Stanger Robert H. Vonderheide | 2012 | Cancer Cell2012，，6： | 2 |
| 5 | A Facile One Pot Microwave -assisted Solid Phase Synthesis of 2-Amino-4,6- diaryl-pyrimidines and Their Antibacterial Activity 显示文摘 | Jhala Y S Gahlot U S Dulawat S S | 2006 | Heterocycl Commun2006，12，34： | 1 |
| 6 | Solid pseudopapillary tumor of the pancreas: a review of salient clinical and pathologic features显示文摘 | Adams AL Siegal GP Jhala NC | 2008 | Adv Anat Pathol2008，15，1： | 1 |
| 7 | Infiltration of Helicobacter pylori in the gastric mucosa显示文摘 | Jhala NC Siegal GR Klemm K | 2003 | Am J Clin Pathol2003，119，： | 1 |
| 8 | The software model checker Blast显示文摘 | Beyer D Henzinger T A Jhala R | 2007 | STTT2007，9，56： | 1 |
| 9 | Fluoride and/or arsenic toxicity in mice testis with formation of giant cells and subsequent recovery by some antidotes 显示文摘 | Chinoy NJ Tewari K Jhala DD | 2004 | Fluoride2004，37，3： | 1 |
| 10 | Endoscopic lumbar discectomy : experience of first 100 cases显示文摘 | Jhala A Mistry M | 2010 | Indian J Orthop2010，2，： | 1 |
| 11 | SCF(beta-TrCP1) controis Smad 4 protein stability in pancreatic cancer cells显示文摘 | HUANG J JHALA N C | 2005 | Am J Patbol2005，166，5： | 1 |
| 12 | Solid pseudopapillary tumor of the pancreas:a review of salient clinical and pathologic features 显示文摘 | Adams AL Siegal GP Jhala NC | 2008 | Adv Anat Pathol2008，15，1： | 1 |
| 13 | Genetic characterization ofIndian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments 显示文摘 | Kerur N Jhala MK Joshi CG | 2008 | Research in Veterinary Science2008，85，： | 1 |
| 14 | Fine needle aspirafionbiopsy of the islet cell tumor of pancreas: a comparison betweecomputerized axial tomography and endoscopic ultrasound-guided fine needle aspiration biopsy显示文摘 | JHALA D ELOUBEIDI M CHHIENG DC | 2002 | Ann Diagn Pathol2002，6，： | 1 |
| 15 | Endoscopic ultrasound-guided fine-needle aspiration biopsy of the adrenal glands: analysis of 24 patients 显示文摘 | JHALA NC JHALA D ELOUBEIDI MA | 2004 | Cancer2004，102，： | 1 |
| 16 | Non-Hodgkin's lymphoma f the breast: a review of 18 primal' and seconda cases 显示文摘 | I)uncanVE Reddy V Jhala NC et hi | 2006 | Annals Diag Pathol2006，10，3： | 1 |
| 17 | Fine needle aspiration diagnosis of Rosai-Dorfman disease in an osteolytic lesion of bone显示文摘 | Li S Yan Z Jhala N | 2010 | Cytojournal2010，7，： | 1 |
| 18 | Software Model Checking显示文摘 | Ranjit Jhala Rupak Majumdar | 2009 | ACM Computing Surveys (CSUR)2009，，： | 1 |
| 19 | Molecular characterization of B-Lβ Ⅱ family(MHC class Ⅱ)alleles in three strains of poultry and its association with immune response显示文摘 | SHANAZ S S JOSH C G JHALA M K | 2005 | Poult Sci2005，40，1： | 1 |
| 20 | Non-Hodgkin lymphoma of the breast: a review of 18 primary and secondary cases显示文摘 | Duncan VE Reddv VV Jhala NC | 2006 | Ann Diagn Pathol2006，10，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员