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| 1 | Anti-allergic and anti-microbial activities of some Thai crops显示文摘 | Tewtrakul S Itharat A Thammaratwasik P Ooraikul B | | 0，，04： | 1 |
| 2 | Antioxidant activities and phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts显示文摘OBJECTIVE: To investigate the antioxidant activities as well as phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts.METHODS: The leaves of A. thwaitesianum were extracted using three different methods: blending with distilled water, maceration with ethanol and decoction. The chemical antioxidant activity of the plant leaf extracts was evaluated using 2,2-diphenyl-1-picryhydrazyl(DPPH) radical and 2,2′-azinobis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt(ABTS+) radical scavenging assays, as well as the ferric reducing antioxidant power assay. Cellular antioxidant activity was determined by superoxide and nitric oxide scavenging assays. The cytotoxicity of the leaf extracts in RAW 264.7 and differentiated HL-60 cells was tested in parallel using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assays, respectively. The total phenolic and flavonoid contents were also assessed by spectrophotometric analysis. Phytochemical constituents of the most potent extract were investigated by liquid chromatography with an electrospray ionization quadrupole time-of-flight mass spectrometer(LC-ESI-QTOF-MS/MS).RESULTS: The ethanolic(ME) and decoction(LW) extracts of dried leaves had the highest chemical scavenging activity against DPPH and ABTS+ free radicals with half maximal effective concentration(EC50) values ranging from 3.54 to 6.44 μg/mL. ME and LW exerted moderate ferric reducing activity, with ferric reducing antioxidant power values of 847.41 and 941.26 mg Fe2+/g extract, respectively. Similarly, ME showed potent cellular scavenging activity against superoxide and nitric oxide radicals with EC50 values of 58.12 and 71.90 μg/mL, respectively. However, LW exhibited only strong nitric oxide scavenging activity with an EC50 value of 91.20 μg/mL. The cell viability of RAW 264.7 and HL-60 cells was greater than 70% in all tested concentrations of both extracts, thus confirming the absence of their cytotoxicity. ME and LW contained high total phenolic contents of 231.14 and 274.42 mg gallic acid equivalents per gram, respectively, as well as high total flavonoid contents of 18.82 and 22.17 mg quercetin equivalents per gram, respectively. LC-ESI-QTOFMS/MS analysis revealed the presence of 52 structurally characterized compounds in ME, 43 of whichwere tentatively identified. Hydroxycinnamic acids such as caffeic acid and its derivatives were the predominant phenolic compounds.CONCLUSION: This is the first report describing potent chemical and cellular antioxidant effects of the ethanolic leaf extract of A. thwaitesianum. The extract contained high total phenolic and flavonoid contents. LC-ESI-QTOF-MS/MS analysis further revealed an abundance of caffeic acid derivatives and flavonoids. These data support its potential use as dietary supplements in oxidative stress prevention. | Bhanuz Dechayont Arunporn Itharat Pathompong Phuaklee Jitpisute Chunthorng-Orn Thana Juckmeta Nuntika Prommee Nitra Nuengchamnong Pintusorn Hansakul | 2017 | Journal of Integrative Medicine2017，15，4： | 1 |
| 3 | Anti-allergic substances from the rhizomes of Dioscorea membranacea显示文摘 | Tewtrakul S Itharat A | | 0，，24： | 1 |
| 4 | Anti-HIV-1 protease- and HIV-1 integrase activities of Thai medicinal plants known as Hua-Khao-Yen 显示文摘 | Tewtrakul S Itharat A Rattanasuwan P | 2006 | J Ethnopharmacol2006，105，12： | 1 |
| 5 | Mulberry leaf extract restores arterial pressure in streptozotocin-induced chronic diabetic rats显示文摘 | Jarinyaporn Naowaboot Patchareewan Pannangpetch Veerapol Kukongviriyapan Upa Kukongviriyapan Saowanee Nakmareong Arunporn Itharat | 2009 | Nutrition Research2009，，： | 1 |
| 6 | Extraction of rotenone from Derris elliptica and Derris malaccensis by pressurized liquid extraction compared with maceration显示文摘 | Sae-Yun A Ovatlamporn C Itharat A | 2006 | J Chromatogr A2006，1125，2： | 1 |
| 7 | Anti-allergic substances from the rhizomes of Dioscorea membranacea显示文摘 | Tewtrakul S Itharat A | 2006 | Bioorganic and Medicinal Chemistry2006，14，11： | 1 |
| 8 | Induction of manganese superoxide dismutase mediates cardiop- rotective effect oftamoxifen显示文摘 | Daosukho C Itharat W Lin SM | 2005 | Journal of Molecular and Cellular Cardiology2005，39，： | 1 |
| 9 | In vitrocytotoxic activity of the medicinal plants used traditionally to treatcancer 显示文摘 | Itharat A Houghton P J Eno- Amooquaye E | 2004 | Journal of Ethnophar Macology2004，90，1： | 1 |
| 10 | Biological activities and chemical content of Sung Yod rice bran oil extracted by expression and soxhlet extraction methods显示文摘 | Uttama S Itharat A Rattarom R | 2014 | J Med Assoc Thai2014，97，8： | 1 |
| 11 | Nitric oxide inhibitory substances from the rhizomes of Dioscorea membranacea 显示文摘 | TEWTRAKUL S ITHARAT A | 2007 | Journal of Ethnopharmacology2007，109，3： | 1 |
| 12 | Induction of manganese superoxide dismutase mediates cardioprotective effect of tamoxifen显示文摘 | Daosukho C Itharat W Lin SM | 2005 | Journal of Molecular and Cellular Cardiology2005，39，： | 1 |
| 13 | In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer显示文摘 | Itharat A Houghton P J Eno-Amooquaye E | 2004 | J Ethnopharmacol2004，90，1： | 1 |
| 14 | Extraction of rotenone from Derris elliptica and Derris malaccensis by pressurized liquid extraction compared with maceration 显示文摘 | SAE-YUN A OVATLARNPORN C ITHARAT A | 2006 | J Chromatogr A2006，1125，2： | 1 |
| 15 | In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer显示文摘 | HOUGHTON P J ENO-AMOOQUAYE E | 2004 | Journal of Ethnopharmacology2004，90，1： | 1 |
| 16 | The Variety Selection and Testing for Effect on HepG-2 Cell Line of Dioscorea membranacea Pierre (Hua Khao Yen Tai) Extract Using Molecular Genetics显示文摘 | Nantana Jaengsuwan Arunporn Itharat | 2014 | Journal of Life Sciences2014，8，4： | 0 |
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