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| 1 | 人乳头瘤病毒6bL1/16E7嵌合蛋白的基因克隆和表达显示文摘目的 研究HPV6bL1/ 16E7嵌合蛋白的基因克隆及其在昆虫细胞的表达 ,为防治尖锐湿疣和宫颈癌的基因工程疫苗研究作准备。方法 用PCR扩增出HPV6bL1/ 16E7嵌合蛋白基因 ,将其克隆到杆状病毒转移载体pVL1393,制备重组杆状病毒并感染昆虫细胞表达HPV6bL1/ 16E7嵌合蛋白。结果 HPV6bL1/ 16E7嵌合蛋白基因在昆虫细胞中得到了表达 ,并可自组装形成病毒样颗粒。结论 昆虫细胞表达的HPV6bL1/ | 张丽芳 陈韶 赵孔南 刘文军 刘小凇 Ian Frazer | 2001 | 中华微生物学和免疫学杂志2001，21，4： | 3 |
| 2 | HPV6b L1 virus like particles are potent immunogens without adjuvant in man 显示文摘 | Lifang Zhang Jian Zhou Ian H Frazer | 2000 | Vaccine2000，18，1112： | 1 |
| 3 | Prophylactic HPV vaccines: Underlying mechanisms显示文摘 | Margaret Stanley Douglas R Lowy Ian Frazer | 2006 | Vaccine2006，24，： | 1 |
| 4 | Tie-in system uses low-cost flanges on deepwater girassol development 显示文摘 | VINCENT ALLIOT IAN FRAZER | 2002 | Oil & Gas Journal2002，，： | 1 |
| 5 | Comparative mapping of human Chromosome 19 with the chicken shows conserved synteny and gives an insight into chromosomal evolution显示文摘 | Jacqueline Smith Ian R. Paton Frazer Murray Richard P.M.A. Crooijmans Martien A.M. Groenen David W. Burt | 2002 | Mammalian Genome2002，，6： | 1 |
| 6 | Tie-ins System Uses Low-cost Flangs on Deepwater Girassol Development 显示文摘 | Vincent Alliot Ian Frazer | 2002 | Oil & Gas Journal2002，，6： | 1 |
| 7 | Construction and Production of FluorescentPapillom avirus-like Particles显示文摘The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has attracted widespread interest since it was demonstrated to be fluorescent in vivo when expressed in other organisms. In order to investigate papillomavirus life cycle which hampered by the unavailability of conventional cell culture system, we constructed a chimeric bovine papillomavirus (BPV) type 1 virus like particles (VLPs) containing GFP. It was found that fluorescent VLPs could be assembled from L2 protein in which GFP is inserted into the N terminal region of L2 (aa 88). The fluorescent VLPs could also be assembled from a GFP/L2 fusion protein in which part of the L2 sequence had been deleted. In vitro , fluorescent VLPs could bind to CV 1 cells, and this VLP/cell interaction could be analyzed by FACS assay. These results demonstrated that GFP could incorporate into BPV1 VLPs without disruption of the VLP structure. Fluorescent VLPs might be a useful tool for study of papillomavirus virus/cell interaction. | PENG Shiwen , ZHOU Jian , Ian H Frazer * Department of Pediatrics, Xiehe Hospital, Tongji Medical University, Wuhan 430022 * Center for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Qld | 1999 | Journal of Huazhong University of Science and Technology(Medical Sciences)1999，19，3： | 0 |
| 8 | The Innovation:A Journal to See the Unseen and Change the Unchanged显示文摘Humanity is standing at the beginning of the Fourth Industrial Revolution and is ready to embrace the digital era.The diversification and specialization of natural science have contributed to re-shaping the society in which we live.Meanwhile,humanity is also facing emergent global challenges,including climate change,emerging infectious diseases,and socioeconomic inequality.The time is ripe to adopt innovative approaches to integrate various disciplines to tackle the issues of sustainable development worldwide. | Ke Chen Tao Huang Enke Liu Yuyuan Lu Zhigang Huo Lei Mi Wei Zhang Ian Frazer | 2020 | The Innovation2020，1，1： | 0 |
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