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| 1 | Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver显示文摘AIM To improve the Cultivation efficiency and yield of human liver cell line CL--l.METHODS High--density cultivation of CL--l onmicrocarriers was carried out with periodicobservation of their growth and proliferation.The specific functions of human liver cell werealso determined.RESULTS Cells of CL-1 cell line grew well onmicrocarrier Cytodex--3 and on the 7th day thepeak was reached. The amount of CLI cells was2.13 x 10’ and the total amount of albuminsynthesis reached 71 .23 ig, urea synthesis23 .32 mg and diazepam transformation 619 .7 ugrespectively. The yield of CL-l on microcarrierswas 49.3 times that of conventional cultivation.The amounts of albumin synthesis, ureasynthesis and diazepam transformation were39.8 times, 41 .6 times and 33.3 times those ofconventional Cultivation, respectively.CONCLUSION The human liver cell line CL--1 canbe Cultivated to a high density with Cytodex--3and has better biological functions. High-densityCultivation of CLI on microcarriers can act asthe biological material of bioartificial liver. | Gao Y Xu XP Hu HZ Yang JZ | 1999 | World Journal of Gastroenterology1999，5，3： | 13 |
| 2 | Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates显示文摘AIM To develop a culture mode providingdurable biomaterials with high yields andactivities used in bioartificial liver.METHODS Hepatocytes were isolated from awhole pig liver by Seglen’s method of orthotopicperfusion with collagenase.In culture onmicrocarriers,primary porcine hepatocyteswere inoculated at a concentration of 5×10~7/mLinto the static culture systems containing 2 g/LCytodex-3,then supplemented with 100 mL/Lfetal calf serum(FCS)or 100 mL/L porcineportal vein serum(PPVS)respectively.Inspheroidal aggregate culture hepatocytes wereinoculated into 100 mL siliconized flasks at aconcentration of 5.0×10~6/mL.RESULTS In culture on microcarriershepatocytes tended to aggregate on Cytodex-3obviously after being inoculated.Typical multi-cellular aggregated spheroids could be found inthe two systems 24 h-48 h after hepatocyteswere cultured.The morphological charact-eristics and synthetic functions were maintainedfor 5 wk in FCS culture system and 8 wk in PPVSculture system.In spheroidal aggregate cultureabout 80%-90% isolated hepatocytes becameaggregated spheroids 24h after cultured insuspension and mean diameter of the spheroidswas 100μm.The relationship among thehepatocytes resembled that in the liver in vivo.Synthetic functions of albumin and urea of the spheroids were twice those of hepatocytescultured on monolayers.CONCLUSION As high-yields and high-activitymodes of culture on microcarriers or inspheroidal aggregate culture with portal veinserum are promising to provide biomaterials forbioartificial liver(BAL)efficiently. | Gao Y Hu HZ Chen K Yang JZ | 2000 | World Journal of Gastroenterology2000，6，3： | 6 |
| 3 | Anoectochilus hainanensis (Orchidaceae),a new species from Hainan,China显示文摘 | Tian HZ Li L Hu AQ | | 0，，： | 1 |
| 4 | Transport distraction os-teogenesis using nitinol spring: An exploration in canine mandible显示文摘 | Zhou HZ Hu M Hu KJ | 2006 | J Craniofac Surg2006，17，5： | 1 |
| 5 | Distribution and chemical coding of corticotropin-releasing factor-immunoreactive neurons in the guinea pig enteric nervous system 显示文摘 | Liu S Gao N Hu HZ et a1 | 2006 | J Comp Neurol2006，494，1： | 1 |
| 6 | Effects of propolis on blood glucose,blood lipid and free radicals in rats with diabetes mellitus显示文摘 | Hu FL Hepbrun HR Xuan HZ | 2005 | Pharmacol Res2005，51，： | 1 |
| 7 | Identification of two critical amino acid res- idues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy 显示文摘 | Qu XX Hao P Song XJ Jiang SM Liu YX Wang PG Rao X Song HD Wang SY Zuo Y Zheng AH Luo M Wang HL Deng F Wang HZ Hu ZH Ding MX Zhao GP Deng HK | 2005 | J Biol Chem2005，280，29： | 1 |
| 8 | Association of lipoprotein-associated phospholipase A2with characteristics of vulnerable coronary atherosclerotic plaques显示文摘 | Liu YS Hu XB Li HZ | 2011 | Yonsei Med J2011，52，6： | 1 |
| 9 | P2X(7) receptors in the enteric nervous system of guinea-pig small intestine显示文摘 | Hu HZ Gao N Lin Z | 2001 | J Comp Neurol2001，440，3： | 1 |
| 10 | Effect of Inhaled Interleukin-4 on Airway Hyperreactivity in Asthmatics 显示文摘 | Shi HZ Deng JM Hu H | 1998 | Am J Respir Crit Care Med1998，157，： | 1 |
| 11 | An alternative splicing product of the murine trpvl gene dominant negatively modulates the activity of TRPV1 channels 显示文摘 | Wang C Hu HZ Colton CK | 2004 | J Biol Chem2004，279，37： | 1 |
| 12 | Neuroimmune interactions in guinea pig stomach and small intestine 显示文摘 | Liu S Hu HZ Gao N | 2003 | Am J Physiol Gastrointest Liver Physiol2003，284，1： | 1 |
| 13 | Validating GWAS-identified risk loci for Alzheimer′s disease in Han Chinese populations 显示文摘 | Wang HZ Bi R Hu QX | 2016 | Mol Neurobiol2016，53，1： | 1 |
| 14 | Transport distraction osteogenesis using nitinol spring:an exploration in canine mandible显示文摘 | Zhou HZ Hu M Hu KJ | 2006 | J Craniofac Surg2006，17，5： | 1 |
| 15 | Depletion of activated alloreactive T cells in prevention of acute graft-versus-host disease显示文摘 | Hu HZ New JY Li B | | 0，，07： | 1 |
| 16 | Anti-inflammatoryand analgesic activities of Edgeworthia chrysanthaand its effective chemical constituents 显示文摘 | Hu XJ Jin HZ Xu WZ | 2008 | BiolPharm Bull2008，31，9： | 1 |
| 17 | Clostridium difficile toxin A excites enteric neurones and suppresses sympathetic neurotransmission in the guinea pig显示文摘 | Xia Y Hu HZ Liu S | 2000 | Gut2000，46，4： | 1 |
| 18 | Grifolin induces apoptosis in cervical car cinoma He La cell line显示文摘 | Hu HZ Luo YH Ni GT | 2009 | Chin J Obst Gynecol2009，1，： | 1 |
| 19 | Short-term simulated nitrogen deposition increases carbon sequestration in a Pleioblastus amarus plantation 显示文摘 | Tu LH Hu TX Zhang J Li RH Dai HZ Luo SH | 2011 | Plant Soil2011，340，12： | 1 |
| 20 | Anal human papillomavirus infection among HIV-infected and uninfected men who have sex with men in Beijing,China显示文摘 | Hu Y Qian HZ Sun J | 2013 | J Acquir Immune Defic Syndr2013，64，1： | 1 |
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