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| 1 | Comprehensive and innovative techniques for livertransplantation in rats: A surgical guide显示文摘AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery. METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis. CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model. | Tomohide Hori Justin H Nguyen Yasuhiro Ogura Toshiyuki Hata Shintaro Yagi Ann-Marie T Baine Norifumi Ohashi Christopher B Eckman Aimee R Herdt Hiroto Egawa Yasutsugu Takada Fumitaka Oike Seisuke Saka-moto Mureo Kasahara Kohei Ogawa Koichiro Hata Taku Iida Yukihide Yonekawa Lena Sibulesky Kagemasa Kuribayashi Takuma Kato Kanako Saito Mie Torii Naruhiko Sahara Naoko Kamo Tomoko Sahara Motohiko Yasutomi Shinji Uemoto | 2010 | World Journal of Gastroenterology2010，16，25： | 14 |
| 2 | Oxidative stress and extracellular matrices after hepatectomy and liver transplantation in rats显示文摘AIM:To investigate oxidative stress(OS)-mediated damage and the behavior of extracellular matrices in various rat models because shear stress with portal hypertension and cold ischemia/warm reperfusion injury trigger the liver regeneration cascade after surgery. These injuries also cause fatal liver damage.METHODS: Rats were divided into four groups according to the surgery performed: control; hepatectomy with 40% liver remnant(60% hepatectomy); orthotopic liver transplantation(OLT) with whole liver graft(100% OLT); and split OLT(SOLT) with 40% graft(40% SOLT). Survival was evaluated. Blood and liver samples were collected at 6 h after surgery. Biochemical and histopathological examinations were performed. OSinduced damage, 4-hydroxynonenal, ataxia-telangiectasia mutated kinase, histone H2AX, phosphatidylinositol 3-kinase(PI3K) and Akt were evaluated by western blotting. Behavior of extracellular matrices, matrix metalloproteinase(MMP)-9, MMP-2, tissue inhibitor of metalloproteinase(TIMP)-1 and TIMP-2 were also evaluated by western blotting and zymography. RESULTS: Although 100% OLT survived, 60% hepatectomy and 40% SOLT showed poor survival. Histopathological, immunohistological, biochemical and protein assays revealed that 60% hepatectomy, 100% OLT and 40% SOLT showed liver damage. PI3K and Akt were decreased in 60% hepatectomy and 40% SOLT. For protein expression, 40% SOLT showed differences in MMP-9, MMP-2 and TIMP-2. TIMP-1 showed differences in 60% hepatectomy and 40% SOLT. For protein activity, MMP-9 demonstrated significant differences in 60% hepatectomy, 100% OLT and 40% SOLT. CONCLUSION: Under conditions with an insufficient liver remnant, prevention of OS-induced damage via the Akt/PI3K pathway may be key to improve the postoperative course. MMP-9 may be also a therapeutic target after surgery. | Tomohide Hori Shinji Uemoto Feng Chen Lindsay B Gardner Ann-Marie T Baine Toshiyuki Hata Takayuki Kogure Justin H Nguyen | 2014 | World Journal of Hepatology2014，6，2： | 7 |
| 3 | Simple and reproducible hepatectomy in the mouse using the clip technique显示文摘AIM: To investigate the reliability of massive hepatectomy models by using clip techniques. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.RESULTS: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques. CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data. | Tomohide Hori Norifumi Ohashi Feng Chen Ann-Marie T Baine Lindsay B Gardner Toshiyuki Hata Shinji Uemoto Justin H Nguyen | 2012 | World Journal of Gastroenterology2012，18，22： | 4 |
| 4 | Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice显示文摘AIM:To investigate the effect of matrix metalloproteinase-9(MMP-9) on the remnant liver after massive hepatectomy in the mouse.METHODS:Age-matched,C57BL/6 wild-type(WT),MMP-9(-/-),and tissue inhibitors of metalloproteinases(TIMP)-1(-/-) mice were used.The mice received 80%-partial hepatectomy(PH).Samples were obtained at 6 h after 80%-PH,and we used histology,immunohistochemical staining,western blotting analysis and zymography to investigate the effect of PH on MMP-9.The role of MMP-9 after PH was investigated using a monoclonal antibody and MMP inhibitor.RESULTS:We examined the remnant liver 6 h after 80%-PH and found that MMP-9 deficiency attenuated the formation of hemorrhage and necrosis.There were significantly fewer and smaller hemorrhagic and necrotic lesions in MMP-9(-/-) remnant livers compared with WT and TIMP-1(-/-) livers(P < 0.01),with no difference between WT and TIMP-1(-/-) mice.Serum alanine aminotransaminase levels were significantly lower in MMP-9(-/-) mice compared with those in TIMP-1(-/-) mice(WT:476 ± 83 IU/L,MMP-9(-/-):392 ± 30 IU/L,TIMP-1(-/-):673 ± 73 IU/L,P < 0.01).Western blotting and gelatin zymography demonstrated a lack of MMP-9 expression and activity in MMP-9(-/-) mice,which was in contrast to WT and TIMP-1(-/-) mice.No change in MMP-2 expression was observed in any of the study groups.Similar to MMP-9(-/-) mice,when WT mice were treated with MMP-9 monoclonal antibody or the synthetic inhibitor GM6001,hemorrhagic and necrotic lesions were significantly smaller and fewer than in control mice(P < 0.05).These results suggest that MMP-9 plays an important role in the development of parenchymal hemorrhage and necrosis in the small remnant liver.CONCLUSION:Successful MMP-9 inhibition attenuates the formation of hemorrhage and necrosis and might be a potential therapy to ameliorate liver injury after massive hepatectomy. | Norifumi Ohashi Tomohide Hori Florence Chen Sura Jermanus Christopher B Eckman Akimasa Nakao Shinji Uemoto Justin H Nguyen | 2012 | World Journal of Gastroenterology2012，18，19： | 3 |
| 5 | Growth inhibitors promote differentiation of insulin producing tissue from embryonic stem cells显示文摘 | Hori Y Rulifson I C Tsai B C | 2002 | Proc Natl Acad Sci USA2002，99，16： | 1 |
| 6 | A numerical study of structural damage detection using changes in the rotation of mode shapes 显示文摘 | Abdo M A B Hori M | 2002 | Journal of Sound and Vibration2002，251，2： | 1 |
| 7 | Optimal aeroassisted or bital interception 显示文摘 | Horie K B Conway A | 1999 | Journal of Guidance Control and Dynamics1999，22，5： | 1 |
| 8 | Preparation and properties of silica-coated cobalt nanoparticles 显示文摘 | KOBAYASHI Y HORIE M KONNO M RODRIGUEZ- GONZALEZ B LIZ-MARZAN L M | 2003 | J Phys Chem B2003，107，30： | 1 |
| 9 | Kinetic parameters in- fluencing the performance of IT-SOFC composite electrodes 显示文摘 | Steele B C H Hori K M Uchino S | 2000 | Solid State Ionics2000，135，14： | 1 |
| 10 | Optimal fighter pursuit-evasion maneuvers found via two-sided optimization 显示文摘 | Horie K Conway B A | 2006 | Journal of Guidance Control and Dynamies2006，29，1： | 1 |
| 11 | Synthetic applications of arylselenenic and arylseleninic acid ,conversion of olefins to allyic alcohols and epoxides显示文摘 | Sharpless B | 1978 | J Org Chem1978，43，： | 1 |
| 12 | Optimal fighter pursuit-evasion in maneuvers found via two-side optimization 显示文摘 | Horie K Conway B A | 2006 | Journal of Guidance Control and Dynamics2006，29，1： | 1 |
| 13 | Intracerebral ganglioglioma显示文摘 | Demierre B Stichnoth F A Hori A | | 0，，02： | 1 |
| 14 | A numerical study of structural damage detection using changes in the rotation of mode shapes显示文摘 | Abdo M A B Hori M | 2002 | Journal of Sound and Vibration2002，251，2： | 1 |
| 15 | A numerical study of structural damage detection using changes in the rotation of mode shapes显示文摘 | Abdo M A- B Hori M | 2002 | Journal of Sound and Vibration2002，251，2： | 1 |
| 16 | Clinical significance of serum soluble intercellular adhesion molecule-1 in gastric cancer显示文摘 | Nakata B Hori T Sunami T | 2000 | Clin Cancer Res2000，6，3： | 1 |
| 17 | Growth inhibitors promote differentiation of insulin-producing tissue from embryonic stem cells显示文摘 | HORI Y RULIFSON I C TSAI B C | 2002 | Proc Natl Acad Sci USA2002，99，16： | 1 |
| 18 | Synthetic applications of arylselenenic and arylseleninic acids, conversion of olefins to allylic alcohols and epoxides显示文摘 | Sharpless K B | 1978 | J Org Chem1978，43，9： | 1 |
| 19 | A new collocation-based method for solving pursuit/evasion ( differential games) problems 显示文摘 | Conway B A Horie K | 2001 | AASJournal2001，445，： | 1 |
| 20 | Clinical significance of serum soluble intercellular adhesion melecule-1 in gastric cancer显示文摘 | Hori T Sunami T | 2001 | Clin Cancer2001，6，3： | 1 |
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