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您的检索式：作者名="Hongnian Guo"
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| 1 | Effect of UTRs from TMV-RNA on the expression of foreign gene in transgenic plants显示文摘To investigate the effects of 5’and 3’ untranslated region (UTR) from tobacco mosaicvirus (TMV) on expression of foreign genes, four plant-expression vectors pBG437, pBG438, pBG440 and pBG440ANOS containing expression cassettes of GUS-NOS; Ω-GUS-NOS, Ω-GUS-3’ UTR-NOS and Ω-GUS-3’ UTR downstream of CaMV 35S promoter with double enhancer sequences respectively have been constructed. Results from a large number of transgenic tobacco plants show that the GUS activity of pBG440 transformed plants is the highest, being 5-fold that of pBG437 and 1.6-fold that of pBG438. Similar results have been obtained in transient expression by injection of Agrobacterium tumefaciens harbouring these constructs into N. benthamiana leaves. These results obtained at the whole plant level confirm the conclusion drawn from the transient expression studies in protoplast system that TMV- Ω fragment can be a translation enhancer and the 3’ UTR has a coordinate effect with ft fragment to enhance foreign gene expression, but | Hongmin Qin Hongnian Guo Yantao Jia Lihong Li Yingchuan Tian | 2000 | Chinese Science Bulletin2000，45，18： | 2 |
| 2 | Construction of a new plant expression vector containing two insect resistant genes and its expression in transgenic tobacco plants显示文摘A new plant expression vector (pBS29K-BA) containing two insect resistant genes, a synthetic chimeric gene BtS29K encoding the activated insecticidal protein Cry1Ac and a gene API-BA encoding the arrowhead (Sagittaria sagittifolia L.) proteinase inhibitor (API) A and B, is constructed. Transgenic tobacco plants expressing these two genes are obtained through Agrobacterium-mediated transformation of tobacco leaf discs. The average expression levels of Cry1Ac and API-BA proteins in transgenic plants are of 3.2 μg and 4.9 μg per gram fresh leaf respectively. The results of insecticidal assay of transgenic plants indicate that the pBS29K-BA transformed plants are more resistant to insect damage than the plants expressing the Cry1Ac gene or API-BA gene alone. | GUO Hongnian, QIN Hongmin, CHEN Xiaoying, LI Changqing, LU Rui and TIAN Yingchuan(Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China) | 2002 | Progress in Natural Science:Materials International2002，12，5： | 1 |
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