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| 1 | Identification of key gene networks controlling organic acid and sugar metabolism during watermelon fruit development by integrating metabolic phenotypes and gene expression profiles显示文摘The organoleptic qualities of watermelon fruit are defined by the sugar and organic acid contents,which undergo considerable variations during development and maturation.The molecular mechanisms underlying these variations remain unclear.In this study,we used transcriptome profiles to investigate the coexpression patterns of gene networks associated with sugar and organic acid metabolism.We identified 3 gene networks/modules containing 2443 genes highly correlated with sugars and organic acids.Within these modules,based on intramodular significance and Reverse Transcription Quantitative polymerase chain reaction(RT-qPCR),we identified 7 genes involved in the metabolism of sugars and organic acids.Among these genes,Cla97C01G000640,Cla97C05G087120 and Cla97C01G018840(r^(2)=0.83 with glucose content)were identified as sugar transporters(SWEET,EDR6 and STP)and Cla97C03G064990(r^(2)=0.92 with sucrose content)was identified as a sucrose synthase from information available for other crops.Similarly,Cla97C07G128420,Cla97C03G068240 and Cla97C01G008870,having strong correlations with malic(r^(2)=0.75)and citric acid(r^(2)=0.85),were annotated as malate and citrate transporters(ALMT7,CS,and ICDH).The expression profiles of these 7 genes in diverse watermelon genotypes revealed consistent patterns of expression variation in various types of watermelon.These findings add significantly to our existing knowledge of sugar and organic acid metabolism in watermelon. | Muhammad Jawad Umer Luqman Bin Safdar Haileslassie Gebremeskel Shengjie Zhao Pingli Yuan Hongju Zhu M.O.Kaseb Muhammad Anees Xuqiang Lu Nan He Chengsheng Gong Wenge Liu | 2020 | Horticulture Research2020，7，1： | 4 |
| 2 | Application and optimization of solid-state fermentation process for enhancing polygalacturonase production by Penicillium expansum显示文摘Penicillium expansum 3.5425 was applied in solid-state fermentation(SSF)of agricultural wastes for polygalacturonase biosynthesis.Among various carbon additives,apple pomace was most suitable for the biosynthesis of polygalacturonase(1440.57 U/g).Optimization of medium parameters using rotational orthogonal design(ROD)experiment combined with optimal fermentation conditions resulted in a 2.72-fold increase in the polygalacturonase production.By using ammonium sulphate precipitation,ion-exchange and gel-permeation chromatography,the polygalacturonase produced by P.expansum 3.5425 was finally purified which had specific activity of 19269 U/mg and molecular weight of 30 kDa.The enzyme was remarkably active in the pH range of 3-5 and at 50℃,which makes it more acceptable in the industrial application.Besides,partially purified polygalacturonase(875.15 U/mL)was used for apple juice clarification and the clarity at 0.4 mL/kg was maximum,which reveals a great potential of polygalacturonase in food industry. | Mingming Zhu Hongju He Mingtao Fan Hanjun Ma Haiwei Ren Jie Zeng Haiyan Gao | 2018 | International Journal of Agricultural and Biological Engineering2018，11，6： | 2 |
| 3 | LW-NIR hyperspectral imaging for rapid prediction of TVC in chicken flesh显示文摘Total viable count(TVC)is often used as an important indicator for chicken freshness evaluation.In this study,112 fresh chicken flesh samples were acquired after slaughtered and their hyperspectral images were collected in the LW-NIR(900-1700 nm)range.The full LW-NIR spectra(486 wavebands)within the images were extracted and applied to related to reference TVC values measured in different storage periods,using partial least squares regression(PLSR)algorithm,resulting in high correlation coefficients(R)and low root mean square errors(RMSE),for either raw spectra or pretreatment spectra.By using regression coefficients(RC)method,20,18,17 and 20 optimal wavebands were respectively selected from raw spectra,baseline correction(BC)spectra,Savitzky-Golay convolution smoothing(SGCS)spectra and standard normal variate(SNV)spectra and applied for the optimization of original full waveband PLSR model.By comparison,RC-PLSR model based on the SGCS spectra showed a better performance in TVC prediction with RC of 0.98 and RMSEC of 0.35 log10 CFU/g in calibration set,and RP of 0.98 and RMSEP of 0.44 log10 CFU/g in prediction set.At last,by transferring the best RC-PLSR model,the dynamic TVC change during the storage was visualized by color maps to indicate the TVC spoilage degree.The overall study revealed that LW-NIR hyperspectral imaging combined with PLSR could be used to predict the freshness of chicken flesh. | Hui Wang Hongju He Hanjun Ma Fusheng Chen Zhuangli Kang Mingming Zhu Zhengrong Wang Shengming Zhao Rongguang Zhu | 2019 | International Journal of Agricultural and Biological Engineering2019，12，3： | 2 |
| 4 | Quick assessment of chicken spoilage based on hyperspectral NIR spectra combined with partial least squares regression显示文摘Pseudomonas spp.and Enterobacteriaceae are dominant spoilage bacteria in chicken during cold storage(0°C-4°C).In this study,high resolution spectra in the range of 900-1700 nm were acquired and preprocessed using Savitzky-Golay convolution smoothing(SGCS),standard normal variate(SNV)and multiplicative scatter correction(MSC),respectively,and then mined using partial least squares(PLS)algorithm to relate to the total counts of Pseudomonas spp.and Enterobacteriaceae(PEC)of fresh chicken breasts to predict PEC rapidly.The results showed that with full 900-1700 nm range wavelength,MSC-PLS model built with MSC spectra performed better than PLS models with other spectra(RAW-PLS,SGCS-PLS,SNV-PLS),with correlation coefficient(RP)of 0.954,root mean square error of prediction(RMSEP)of 0.396 log10 CFU/g and residual predictive deviation(RPD)of 3.33 in prediction set.Based on the 12 optimal wavelengths(902.2 nm,905.5 nm,923.6 nm,938.4 nm,946.7 nm,1025.7 nm,1124.4 nm,1211.6 nm,1269.2 nm,1653.7 nm,1691.8 nm and 1693.4 nm)selected from MSC spectra by successive projections algorithm(SPA),SPA-MSC-PLS model had RP of 0.954,RMSEP of 0.397 log10 CFU/g and RPD of 3.32,similar to MSC-PLS model.The overall study indicated that NIR spectra combined with PLS algorithm could be used to detect the PEC of chicken flesh in a rapid and non-destructive way. | Shengqi Jiang Hongju He Hanjun Ma Fusheng Chen Baocheng Xu Hong Liu Mingming Zhu Zhuangli Kang Shengming Zhao | 2021 | International Journal of Agricultural and Biological Engineering2021，14，1： | 2 |
| 5 | Non-destructive and rapid analysis of moisture distribution in farmed Atlantic salmon (Salmo salar) fillets using visible and near-infrared hyperspectral imaging显示文摘 | He Hongju Di Wu Sun Dawen | 2013 | Innovative Food Science and Emerging Technologies2013，18，： | 1 |
| 6 | Breeding of a New Clubroot-resistant Chinese Cabbage Variety, CCR11242显示文摘In this study,using Japanese radish cytoplasmic sterile line ' Half Green Cabbage'( HGC) and clubroot-resistant South Korean cabbage variety ' CR Cabbage King'( CCK) as experimental materials,self-incompatible DH lines were obtained by microspore culture.Through five generations of backcross,CCR11239,a clubroot-resistant radish cytoplasmic sterile line,was obtained.Through six generations of self-crossing,CCR11240,the maintainer line of CCR11239,was bred.After the cross of CCK and Luchunbai 1( 83- 1) and five generations of self-crossing,self-compatible line CCR11241 was obtained.Through crossing CCR11240 with CCR11241,the new cabbage variety CCR11242 was obtained.After variety comparison test,provincial regional test and production demonstration test,the new variety was registered in 2012.The average yield of CCR11242 reached 60 975 kg / hm2,which was improved by 142% compared with Luchunbai 1( 83- 1).The disease index of CCR11242 was 5.63,which was 88.13 lower than the control,indicating high resistance( HR) to clubroot. | Xuezhong XU Zhimei TIAN Hongli YANG Jingfeng HU Liqin ZHANG Mei LAN Hongju DUAN Jiangming HE | 2016 | Agricultural Biotechnology2016，5，5： | 1 |
| 7 | Mining hyperspectral data for non-destructive and rapid prediction of nitrite content in ham sausages显示文摘Accurate and rapid determination of nitrite contents is an important step for guaranteeing sausage quality.This study attempted to mine hyperspectral data in the range of 900-1700 nm for non-destructive and rapid prediction of nitrite contents in sausages.The average spectra of 156 samples were collected to relate to the measured nitrite values by partial least squares(PLS)regression.Optimal wavelengths were respectively selected by successive projections algorithm(SPA)and regression coefficients(RC)to simplify the PLS model.The results indicated that PLS model established with 15 optimal wavelengths(900.5 nm,907.1 nm,908.8 nm,912.1 nm,915.4 nm,920.3 nm,922.0 nm,941.7 nm,979.6 nm,1083.2 nm,1213.2 nm,1353.0 nm,1460.2 nm,1595.6 nm and 1699.9 nm)selected by SPA had better performance with r C,r CV,r P of 0.92,0.89,0.89 and RMSEC,RMSECV,RMSEP of 0.41 mg/kg,0.89 mg/kg,0.49 mg/kg,respectively,for calibration set,cross-validation and prediction set.It was concluded that hyperspectral data could be mined by PLS&SPA for realizing the rapid evaluation of nitrite content in ham sausages. | Yadong Zhu Hongju He Shengqi Jiang Hanjun Ma Fusheng Chen Baocheng Xu Hong Liu Mingming Zhu Shengming Zhao Zhuangli Kang | 2021 | International Journal of Agricultural and Biological Engineering2021，14，2： | 1 |
| 8 | Identification of aroma compounds in watermelon juice by SPME- GC- MS 显示文摘 | TANG Xiaowei HE Hongju LIU Ye | 2012 | International Society for Horticultural Science2012，944，： | 1 |
| 9 | Grain Boundaries Engineering of Hollow Copper Nanoparticles Enables Highly Efficient Ammonia Electrosynthesis from Nitrate显示文摘Electrochemical nitrate reduction reaction(NO_(3)−RR)is an ideal route to produce ammonia(NH_(3))under ambient conditions.Although a markedly improved NH3 production rate has been achieved on the NO_(3)−RR compared with the nitrogen reduction reaction(NRR),the NH_(3) production rate of NO_(3)−RR is still well below the industrial Haber-Bosch route due to the lack of robust electrocatalysts for yielding high current densitieswith concurrently good suppression of hydrogen evolution reaction(HER).Herein,we describe an in situ electrochemical strategy for the synthesis of hollow carbon-coated Cu nanoparticles(NPs)(HSCu@C)with abundant grain boundaries(HSCu-AGB@C)for highly efficient NO_(3)−RR in both alkaline and neutral media.Impressively,in alkaline media,the HSCu-AGB@C can achieve a maximum NH3 Faradaic efficiency of 94.2% with an ultrahigh NH_(3) rate of 487.8 mmol g^(−1) cat h^(−1) at−0.2 V versus a reversible hydrogen electrode,more than 2.4-fold of the rate obtained in the Haber-Bosch.Both theoretic computations and experimental results uncover that the grain boundaries play the key to improve the NO_(3)−RR performance.Herein,the industrial-scale NH_(3) production ratemay open exciting opportunities for the practical electrosynthesis NH_(3) under ambient conditions. | Qi Hu Yongjie Qin Xiaodeng Wang Hongju Zheng Keru Gao Hengpan Yang Peixin Zhang Minhua Shao Chuanxin He | 2022 | CCS Chemistry2022，4，6： | 1 |
| 10 | Rapid and real-time prediction of lactic acid bacteria (LAB) in farmed salmon flesh using near- infrared (NIR) hyperspectral imaging cmnbined with chemometric analysis显示文摘 | He Hongju Sun Dawen Wu Di | 2014 | Food Research International2014，62，8： | 1 |
| 11 | Toward enhancmnent in prediction of Pseudomonas counts distribution in salmon fillets using NIR hyperspectral imaging显示文摘 | He Hongju Son Dawen | 2015 | LWT-Food Science and Technology2015，62，11： | 1 |
| 12 | Identification ofaroma compounds in watermelon juice by SPME-GCMS显示文摘 | TANG Xiaowei HE Hongju LIU Yey | 2012 | ActaHorticulturae2012，944，： | 1 |
| 13 | Effects of fresh-cut and storage on glucosinolates profile using broccoli as a case study显示文摘Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli. | Xiaoxin Huang Bing Cheng Yaqin Wang Guangmin Liu Liping Hu Xiaolu Yu Hongju He | 2023 | Horticultural Plant Journal2023，9，2： | 1 |
| 14 | Nondestructive determination of IMP content in chilled chicken based on hyperspectral data combined with chemometrics显示文摘This study was conducted to investigate the potential of hyperspectral imaging technique(900-1700 nm)for nondestructive determination of inosinic acid(IMP)in chicken.Hyperspectral images of chicken flesh samples were acquired,and their mean spectra within the images were extracted.The quantitative relationship between the mean spectra and reference IMP value was fitted by partial least squares(PLS)regression algorithm.A PLS model(MAS-PLS)built with moving average smoothing(MAS)spectra showed better performance in predicting IMP content,leading to correlation coefficients(RP)of 0.951,root mean square error(RMSEP)of 0.046 mg/g,and residual predictive deviation(RPD)of 3.152.Regression coefficient(RC),successive projections algorithm(SPA),stepwise,competitive adaptive reweighted sampling(CARS),and uninformative variable elimination(UVE)were used to select the optimal wavelengths to optimize the MAS-PLS model.Based on the 18 optimal wavelengths(907.14,917.02,918.67,926.90,930.20,936.78,956.54,1004.28,1135.89,1211.56,1302.07,1367.94,1397.60,1488.31,1680.17,1683.49,1686.80 and 1695.10 nm)selected from MAS spectra by SPA,the MAS-SPA-PLS model was built with R_(P) of 0.920,RMSEP of 0.056 mg/g and RPD of 3.220,which was similar to the MAS-PLS model.The overall study indicated that hyperspectral imaging in the 900-1700 nm range combined with PLS and SPA could be used to predict the IMP content in chicken flesh. | Yangyang Wang Hongju He Shengqi Jiang Hanjun Ma | 2022 | International Journal of Agricultural and Biological Engineering2022，15，1： | 0 |
| 15 | Recoloring tomato fruit by CRISPR/Cas9-mediated multiplex gene editing显示文摘Fruit color is an important horticultural trait,which greatly affects consumer preferences.In tomato,fruit color is determined by the accumulation of different pigments,such as carotenoids in the pericarp and f lavonoids in the peel,along with the degradation of chlorophyll during fruit ripening.Since fruit color is a multigenic trait,it takes years to introgress all color-related genes in a single genetic background via traditional crossbreeding,and the avoidance of linkage drag during this process is difficult.Here,we proposed a rapid breeding strategy to generate tomato lines with different colored fruits from red-fruited materials by CRISPR/Cas9-mediated multiplex gene editing of three fruit color-related genes(PSY1,MYB12,and SGR1).Using this strategy,the red-fruited cultivar‘Ailsa Craig’has been engineered to a series of tomato genotypes with different fruit colors,including yellow,brown,pink,light-yellow,pink-brown,yellow-green,and light green.Compared with traditional crossbreeding,this strategy requires less time and can obtain transgene-free plants with different colored fruits in less than 1 year.Most importantly,it does not alter other important agronomic traits,like yield and fruit quality.Our strategy has great practical potential for tomato breeding and serves as a reference for improving multigene-controlled traits of horticultural crops. | Tianxia Yang Muhammad Ali Lihao Lin Ping Li Hongju He Qiang Zhu Chuanlong Sun Ning Wu Xiaofei Zhang Tingting Huang Chang-Bao Li Chuanyou Li Lei Deng | 2023 | Horticulture Research2023，10，1： | 0 |
| 16 | Glucosinolate Variation in Turnip (Brassica rapa L.) at Different Growing Stages显示文摘The seed,leaf and root parts of three turnip( Brassica rapa L.) cultivars were analyzed for glucosinolates. The component and concentration of glucosinolates in the three cultivars were also analyzed at different growing stages. Eight kinds of glucosinolates were tested in turnip. They were PRO,NAP,4 OH,GBN,GBC,NAS,4 ME and NEO,respectively. The contents and kinds of glucosinolates varied at different growing stages. The content of NAS was the highest in the seed,GBC and NEO contents were the highest in the seedling,GBN and NEO contents were the highest in the leaf,while the highest NAS content was in the root. | Xuezhi ZHAO Hongju HE Pangyuan LIU Wenqi WANG Shuhui SONG Xiangbing MENG | 2018 | Agricultural Biotechnology2018，7，4： | 0 |
| 17 | Watermelon domestication was shaped by stepwise selection and regulation of the metabolome显示文摘Although crop domestication has greatly aided human civilization,the sequential domestication and regulation of most quality traits remain poorly understood.Here,we report the stepwise selection and regulation of major fruit quality traits that occurred during watermelon evolution.The levels of fruit cucurbitacins and flavonoids were negatively selected during speciation,whereas sugar and carotenoid contents were positively selected during domestication.Interestingly,fruit malic acid and citric acid showed the opposite selection trends during the improvement.We identified a novel gene cluster(CGC1,cucurbitacin gene cluster on chromosome 1)containing both regulatory and structural genes involved in cucurbitacin biosynthesis,which revealed a cascade of transcriptional regulation operating mechanisms.In the CGC1,an allele caused a single nucleotide change in Cl ERF1 binding sites(GCC-box)in the promoter of Cl Bh1,which resulted in reduced expression of Cl Bh1 and inhibition of cucurbitacin synthesis in cultivated watermelon.Functional analysis revealed that a rare insertion of 244 amino acids,which arose in C.amarus and became fixed in sweet watermelon,in Cl OSC(oxidosqualene cyclase)was critical for the negative selection of cucurbitacins during watermelon evolution.This research provides an important resource for metabolomics-assisted breeding in watermelon and for exploring metabolic pathway regulation mechanisms. | Pingli Yuan Congping Xu Nan He Xuqiang Lu Xingping Zhang Jianli Shang Hongju Zhu Chengsheng Gong Hanhui Kuang Tang Tang Yong Xu Shuangwu Ma Dexi Sun Weiqin Zhang Muhammad J.Umer Jian Shi Alisdair R.Fernie Wenge Liu Jie Luo | 2023 | Science China(Life Sciences)2023，66，3： | 0 |
| 18 | Analysis on Composition and Content of Glucosinolates in Broccoli Flowers and Leaves显示文摘Glucosinolate composition and content were evaluated in flowers and leaves of 12 different broccoli varieties. The results indicated that there were 9 glucosinolates in broccoli,namely Glucoiberin(IBE); Progoitrin(PRO);Sinigrin(SIN);Glucoraphanin(RAA);Gluconapin(NAP);4-Hydroxyglucobrassicin(4OH);Glucobrassicin(GBC);4-Methoxyglucobrassicin(4ME);Neoglucobrassicin(NEO).Total glucosinolate content in flowers was 1-5 times higher than in leaves. The predominant glucosinolate in broccoli was glucoraphanin. | Guangmin LIU Yue MA Yunhua DING Liping HU Xuezhi ZHAO Hongju HE | 2017 | Asian Agricultural Research2017，9，4： | 0 |
| 19 | Nanostructures and catalytic atoms engineering of tellurium-based materials and their roles in electrochemical energy conversion显示文摘With the dramatic developments of renewable and environmental-friendly electrochemical energy conversion systems,there is an urgent need to fabricate durable and efficient electrocatalysts to address the limitation of high overpotentials exceeding thermodynamic requirements to facilitate practical applications.Recently,tellurium-based nanomaterials(Te NMs)with unique chemical,electronic,and topological properties,including Te-derived nanostructures and transition metal tellurides(TMTs),have emerged as one of the most promising electrocatalytic materials.In the absence of comprehensive and guiding reviews,this review comprehensively summarizes the main advances in designing emerging Te NMs for electrocatalysis.First,the engineering strategies and principles of Te NMs to enhance their electrocatalytic activity and stability from the nanostructures to the catalytic atoms are discussed in detail,especially on the chemical/physical/multiplex templating strategies,heteroatom doping,vacancy/defect engineering,phase engineering,and the corresponding mechanisms and structure-performance correlations.Then,typical applications of Te NMs in electrocatalysis are also discussed in detail.Finally,the existing key issues and main challenges of Te NMs for electrocatalysis are highlighted,and the development trend of Te NMs as electrocatalysts is expounded.This review provides new concepts to guide future directions for developing Te NMs-based electrocatalysts,thereby promoting their future wide applications in electrochemical energy systems. | Tiantian Li Yuting Deng Xiao Rong Chao He Mi Zhou Yuanjiao Tang Hongju Zhou Chong Cheng Changsheng Zhao | 2023 | SmartMat2023，4，1： | 0 |
| 20 | Studies on the Simultaneous Determination of Cr(Ⅲ)and Cr(Ⅵ)by Ion Chromatography(IC)显示文摘The research aimed to develop and validate an ion chromatography method for the simultaneous analysis of Cr(Ⅲ) and Cr(Ⅵ)from pumpkin. A new analytical method based on ion chromatography techniques was developed by the Cr(Ⅲ) pre-column derivatization and Cr(Ⅵ) post-column derivatization. The ion chromatography condition was optimized and the detection sensitivity was improved. Cr(Ⅲ) and Cr(Ⅵ) were determined by 365 and 530 nm,respectively. The temperature of water bath,the heating time for pre-column derivatization,and the flow rate of post-column derivative liquid were screened on the basis of single factor experiment,the effects of various factors were determined by the method of L16( 43) orthogonal experiment design. Considering the results of orthogonal experiments and the variation tendency of peak area under different factors,the optimum derivatization conditions were chose as follows: the flow rate of post-column derivative liquid is0. 5 m L/min,the temperature of water bath for pre-column derivatization is 100 ℃ and the heating time is 5 min. The conditions were optimized by means of orthogonal experiments under the p H of leacheate ranged from 6. 5 to 6. 8 and the I-concentration of 5 mmol/L. Under optimized derivatization conditions,the detection limits for Cr(Ⅲ) and Cr(Ⅵ) were 0. 17 and 0. 019 mg,respectively. And the average recoveries of Cr(Ⅲ) in pumpkin were in the range of 82%-85%. Results indicated that pumpkin does not contain Cr(Ⅵ) according to this ion chromatography method of simultaneously determination for Cr(Ⅲ) and Cr(Ⅵ). | Guangmin LIU Xin LIU Feng GAO Na SUN Liping HU Yaqin WANG Hongju HE | 2017 | Asian Agricultural Research2017，9，5： | 0 |
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