|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The role of gut microbiota(commensal bacteria)and the mucosal barrier in the pathogenesis of inflammatory and autoimmune diseases and cancer:contribution of germ-free and gnotobiotic animal models of human diseases显示文摘Metagenomic approaches are currently being used to decipher the genome of the microbiota(microbiome),and,in parallel,functional studies are being performed to analyze the effects of the microbiota on the host.Gnotobiological methods are an indispensable tool for studying the consequences of bacterial colonization.Animals used as models of human diseases can be maintained in sterile conditions(isolators used for germ-free rearing)and specifically colonized with defined microbes(including non-cultivable commensal bacteria).The effects of the germ-free state or the effects of colonization on disease initiation and maintenance can be observed in these models.Using this approach we demonstrated direct involvement of components of the microbiota in chronic intestinal inflammation and development of colonic neoplasia(i.e.,using models of human inflammatory bowel disease and colorectal carcinoma).In contrast,a protective effect of microbiota colonization was demonstrated for the development of autoimmune diabetes in non-obese diabetic(NOD)mice.Interestingly,the development of atherosclerosis in germ-free apolipoprotein E(ApoE)-deficient mice fed by a standard low-cholesterol diet is accelerated compared with conventionally reared animals.Mucosal induction of tolerance to allergen Bet v1 was not influenced by the presence or absence of microbiota.Identification of components of the microbiota and elucidation of the molecular mechanisms of their action in inducing pathological changes or exerting beneficial,disease-protective activities could aid in our ability to influence the composition of the microbiota and to find bacterial strains and components(e.g.,probiotics and prebiotics)whose administration may aid in disease prevention and treatment. | Helena Tlaskalova-Hogenova Renata Stepankova Hana Kozakova Tomas Hudcovic Luca Vannucci Ludmila Tuckova Pavel Rossmann TomasHrncır Miloslav Kverka Zuzana Zakostelska Klara Klimesova Jaroslava Pribylova Jirina Bartova Daniel Sanchez Petra Fundova Dana Borovska Dagmar Sru˚tkova Zdenek Zıdek Martin Schwarzer Pavel Drastich David P Funda | 2011 | Cellular & Molecular Immunology2011，8，2： | 47 |
| 2 | Th1 cytokines promote T-cell binding to antigen-presenting cells via enhanced hyaluronan production and accumulation at the immune synapse显示文摘Hyaluronan(HA)production by dendritic cells(DCs)is known to promote antigen presentation and to augment T-cell activation and proliferation.We hypothesized that pericellular HA can function as intercellular‘glue’directly mediating T cell–DC binding.Using primary human cells,we observed HA-dependent binding between T cells and DCs,which was abrogated upon pre-treatment of the DCs with 4-methylumbelliferone(4-MU),an agent which blocks HA synthesis.Furthermore,T cells regulate HA production by DCs via T cell-derived cytokines in a T helper(Th)subset-specific manner,as demonstrated by the observation that cell-culture supernatants from Th1 but not Th2 clones promote HA production.Similar effects were seen upon the addition of exogenous Th1 cytokines,IL-2,interferon c(IFN-c)and tumor necrosis factor a(TNF-a).The critical factors which determined the extent of DC–T cell binding in this system were the nature of the pre-treatment the DCs received and their capacity to synthesize HA,as T-cell clones which were pre-treated with monensin,added to block cytokine secretion,bound equivalently irrespective of their Th subset.These data support the existence of a feedforward loop wherein T-cell cytokines influence DC production of HA,which in turn affects the extent of DC–T cell binding.We also document the presence of focal deposits of HA at the immune synapse between T-cells and APC and on dendritic processes thought to be important in antigen presentation.These data point to a pivotal role for HA in DC–T cell interactions at the IS. | Paul L Bollyky Stephen P Evanko Rebecca P Wu Susan Potter-Perigo S Alice Long Brian Kinsella Helena Reijonen Kelly Guebtner Brandon Teng Christina K Chan Kathy R Braun John A Gebe Gerald T Nepom Thomas N Wight | 2010 | Cellular & Molecular Immunology2010，7，3： | 3 |
| 3 | Genetic ancestry analysis in non-alcoholic fatty liver disease patients from Brazil and Portugal显示文摘AIM:To study the association between genetic ancestry,non-alcoholic fatty liver disease(NAFLD) metabolic characteristics in two cohorts of patients,from Brazil and Portugal. METHODS:We included 131 subjects from Brazil [(n = 45 with simple steatosis(S. Steatosis) and n = 86 with nonalcoholic steatohepatitis(NASH)] and 90 patients from Portugal(n = 66,S. Steatosis; n = 24,NASH). All patients had biopsy-proven NAFLD. In histologic evaluation NAFLD activity score was used to assess histology and more than 5 points defined NASH in this study. Patients were divided into two groups according to histology diagnosis:simple steatosis or non-alcoholic statohepatitis. Genetic ancestry was assessed using real-time polymerase chain reaction. Seven ancestry informative markers(AT3-I/D,LPL,Sb19.3,APO,FYNull,PV92,and CKMM) with the greatest ethnicgeographical differential frequencies(≥ 48%) were used to define genetic ancestry. Data were analyzed using R PROJECTS software. Ancestry allele frequencies between groups were analyzed by GENEPOP onlineand the estimation of genetic ancestry contribution was evaluated by ADMIX-95 software. The 5% alpha-error was considered as significant(P < 0.05). RESULTS:In the Brazilian sample,NASH was significantly more frequent among the elderly patients with diabetes(NASH 56 ± 1.1 years old vs S. Steatosis 51 ± 1.5 years old,P = 3.7 x 10-9),dyslipidemia(NASH 63% vs S. Steatosis 37%,P = 0.009),higher fasting glucose levels(NASH 124 ± 5.2 vs S. Steatosis 106 ± 5.3,P = 0.001) and Homeostatic Model of Assessment index > 2.5 [NASH 5.3(70.8%) vs S. Steatosis 4.6(29.2%) P = 0.04]. In the Portuguese study population,dyslipidemia was present in all patients with NASH(P = 0.03) and hypertension was present in a larger percentage of subjects in the S. Steatosis group(P = 0.003,respectively). The genetic ancestry contribution among Brazilian and Portuguese individuals with NASH was similar to those with S. Steatosis from each cohort(Brazilian cohort:P = 0.75; Portuguese cohort:P = 0.97). Nonetheless,the genetic ancestry contribution of the Brazilian and Portuguese population were different,and a greater European and Amerindian ancestry contribution was detected in the Portuguese population while a higher African genetic ancestry contribution was observed in Brazilian population of both NASH and S. Steatosis groups.CONCLUSION:There was no difference between the genetic ancestry contribution among Brazilian and Portuguese individuals with NASH and S. Steatosis from each cohort. | Lourianne Nascimento Cavalcante Jose Tadeu Stefano Mariana V Machado Daniel F Mazo Fabiola Rabelo Kiyoko Abe Sandes Flair Jose Carrilho Helena Cortez-Pinto Andre Castro Lyra Claudia P de Oliveira | 2015 | World Journal of Hepatology2015，7，10： | 2 |
| 4 | The effect of dietary protein replacement by crystalline amino acid on growth and nitrogen utilization of turbot Scophthalmus mazimus juveniles 显示文摘 | Helena P Aires O T | 2005 | Aquaculture2005，250，75： | 1 |
| 5 | Behaviors and Hygiene Habits of Complete Denture Wearers 显示文摘 | Amanda P Ingrid A Helena P | 2010 | Braz Dent J2010，21，3： | 1 |
| 6 | Characterisati- on of 19 almond cuhivars on the basis of their free amino acids composition显示文摘 | Helena S L Poveda E G Moya M S P | 1998 | Food Chemistry1998，61，4： | 1 |
| 7 | Lysine requirement and efficiency of lysine utilization in turbot (Scophthalmus maximus) juveniles显示文摘 | HELENA P AIRES O T | 2008 | Aquaculture2008，275，： | 1 |
| 8 | Partially esterified sucrose derivatives:Synthesis of 6-acetyl -2,3,4-tri-O- sucrose,a naturally occurring flavour precursor of tobacco显示文摘 | Garegg P J Stefan Oscarson Helena Ritzen | 1988 | Carbohy drate Research1988，，181： | 1 |
| 9 | The influence of layered compounds on the properties of starch/layered compound composites 显示文摘 | Helena - M Wilhelm Maria - R Sierakowski Gabriel P Souza | 2003 | Polymer International2003，52，： | 1 |
| 10 | Changes in the cell membrance of lactobacillus bulgaricus during storage following freeze-drying显示文摘 | PAULA M | 1996 | Biotechnology Letters1996，18，： | 1 |
| 11 | Study of vertical transmission of fowl adenoviruses显示文摘 | HELENA G CYNTHIA P DAVPR O | 2006 | Can J Vet Res2006，70，： | 1 |
| 12 | Highly porous and interconnected starch-based scaffolds Production,characterization and surface modification显示文摘 | Iva P Paula M L P Azevedo Helena S A | 2010 | Materials Science and Engineering C2010，30，： | 1 |
| 13 | Serologic monitoring of a broiler breeder flock previously affected by inclusion body hepatitis and testing of the progeny for vertical transmission of fowl adenoviruses显示文摘 | CYNTHIA P HELENA G DAVOR O | 2007 | Can J Vet Res2007，71，： | 1 |
| 14 | Enantioselective transport and CYP3A4-mediated metabolism of R/S-verapamil in Caco-2 cell monolayers显示文摘 | Helena E Christer T Artursson P | 2003 | Eur J Pharm Sci2003，19，： | 1 |
| 15 | Improvement of tech- nological quality of eucalypt wood by heat treatment in air at 170-200℃显示文摘 | BRUNO E IDALINA D HELENA P | 2007 | Forest Products Journal2007，57，： | 1 |
| 16 | Psychological care in nursing education and practice:a research for definition and dimension显示文摘 | Helena P | 1999 | Nurse education today1999，19，1： | 1 |
| 17 | The effect of dietary protein replacement by crystalline amino acid on growth and nitrogen utilization of turbot Scophthalmus maximus juveniles 显示文摘 | Helena P Aires O T | 2005 | Aquaculture2005，250，： | 1 |
| 18 | Microbial degradation of low rank coals显示文摘 | HELENA M KAMILA P ANNA P | 2002 | Fuel Processing Technology2002，77，78： | 1 |
| 19 | Metabolicinteractions between ethanol and MDMA in primary culturedrat hepatocytes显示文摘 | Helena P Paula G P Eduarda F | 2010 | Toxicology2010，270，23： | 1 |
| 20 | Genes of laminin β1chain, α1 (IV) chain of type Ⅳ collagen, and 72 - kDa,type Ⅳ collagenase are mainly expresed by the stromal cells of lung carcinomas 显示文摘 | Ylermi S Paavo P helena AH | 1993 | Am JPathol1993，142，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员