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83篇 您的检索式：作者名="Helbig H"

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	题名	作者	年代	出处	被引量
1	Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC.	Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard	2012	World Journal of Gastroenterology2012，18，26：	12
2	Occurrence and distribution of sequence types among Legiortella pneumophila strains isolated from patiens in Germany: common features and differences to other regions of the world显示文摘	BORCHARDT J HELBIG J H LUCK P C	2008	Eur J Clin microbial Infect Dis2008，27，1：	1
3	The role of action representation in visual object显示文摘	Helbig H B Graf M Kiefer M		0，，：	1
4	Rubeosis iridis after vitrectomy for diabetic retinopathy 显示文摘	Helbig H Kellner U Bornfeld N	1998	Graefe''s Arch Clin Exp Ophthalmol1998，236，10：	1
5	Surgery for diabetic retinopathy显示文摘	Helbig H	2007	Ophthalmologica2007，221，2：	1
6	Core de- compression combined with impl-antation of a deminer- alised bone matrix for non-traumatic osteonecrosis of the fern-oral head显示文摘	HELBIG L SIMANK H KROEBER M	2012	Arch Orthop Trauma Surg2012，132，8：	1
7	Choroidal thick- ness measurements during central serous chorioretinopathy treatment显示文摘	BRANDL C HELBIG H GAMULESCU MA	2014	Int Ophthalmol2014，34，1：	1
8	Time - variant non - linear phase - coupling analysis of EEG burst patterns in sedated patients during electroencephalic burst suppression period显示文摘	Schack B Witte H Helbig M		Clinical neurophysiology0，112，8：	1
9	Detection of Legionella pneumophila antigen in urine samples by the BinaxNOW immunochromatographic assay and comparison with both Binax Legionella Urinary Enzyme Immunoassay (EIA) and Biotest Legionella Urin Antigen EIA显示文摘	Uldum S A Luck P C	2001	J Med Microbiol2001，50，6：	1
10	Evaluation of published recommendations for screening studies of retinopathy of prematurity显示文摘	Kellner U Jardeck C Helbig H	1995	Ophthalmology1995，92，5：	1
11	A system for automatic electrical and optical characterization of microelectromechanical devices显示文摘	HELBIG H F	1999	Microelectromech Syst1999，8，4：	1
12	Pseudophakic retinal detachment显示文摘	Herrmann W Helbig H Heimann H	2011	Klinische Monatsbltter für Augenheilkunde2011，228，3：	1
13	Surgery for diabetic retinopathy显示文摘	Helbig H	2007	Ophthalmologica2007，221，2：	1
14	Diabetic eye disease 显示文摘	Prahs P Helbig H	2009	Ther Umsch2009，66，3：	1
15	A system for automatic electrical and optical characterization of microelectromechanical devices 显示文摘	Bums D J Helbig H F	1999	Journal of Microelectromechanical Systems1999，8，4：	1
16	Insulin-induced hyperpolarization in retinal capillary pericytes显示文摘	Berweck S Thieme H Lepple-Wienhues A Helbig H Wiederholt M	1993	Invest Ophthalmol Vis Sci1993，34，12：	1
17	Effect of Oxygen Deprivation on Soil Hydrophobicity during Heating 显示文摘	Bryant R Doerr S H Helbig M	2005	International Journal of Wildland Fire2005，14，：	1
18	Familial retinal arteriolar tortuosity: a re- view显示文摘	Sutter FK Helbig H	2003	Surv Ophthalmol2003，48，3：	1
19	Uveal effusion syndrome显示文摘	Valmaggia C Helbig H Fretz C	2007	Klin Monatsbl Augenheilkd2007，224，：	1
20	Epitope mapping of immunogenic and adhesive structures in repetitive domains of- Mycoplasma boris variable surface lipoprot eins显示文摘	Sachse K Helbig J H Lysnyans ky I	2000	Infect Im- mun2000，68，2：	1

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