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| 1 | Chondroitinase ABC plus bone marrow mesenchymal stem cells for repair of spinal cord injury显示文摘As chondroitinase ABC can improve the hostile microenvironment and cell transplantation is proven to be effective after spinal cord injury, we hypothesized that their combination would be a more effective treatment option. At 5 days after T8 spinal cord crush injury, rats were injected with bone marrow mesenchymal stem cell suspension or chondroitinase ABC 1 mm from the edge of spinal cord damage zone. Chondroitinase ABC was first injected, and bone marrow mesenchymal stem cell suspension was injected on the next day in the combination group. At 14 days, the mean Basso, Beattie and Bresnahan score of the rats in the combination group was higher than other groups. Hematoxylin-eosin staining showed that the necrotic area was significantly reduced in the combination group compared with other groups. Glial fibrillary acidic protein-chondroitin sulfate proteoglycan double staining showed that the damage zone of astrocytic scars was significantly reduced without the cavity in the combination group. Glial fibrillary acidic protein/growth associated protein-43 double immunostaining revealed that positive fibers traversed the damage zone in the combination group. These results suggest that the combination of chondroitinase ABC and bone marrow mesenchymal stem cell transplantation contributes to the repair of spinal cord injury. | Chun Zhang Xijing He Haopeng Li Guoyu Wang | 2013 | Neural Regeneration Research2013，8，11： | 10 |
| 2 | Influence of early postoperative complication on the operative results in elderly intertrochanteric fractured patients显示文摘客观:早复杂并发症与与操作或非起作用的途径对待的 intertrochanteric 骨折的 Evans Ⅲ类型在那些被分析。有在到 2006 年 7 月的 1999 年 6 月之间的 intertrochanteric 骨折的 Evans Ⅲ类型的 Methods:59 盒子被同意进入我们的部门。38 个病人与心血管的疾病是复杂的,例如高血压和心律不齐。所有盒子被操作。Results:58 盒子成功地被操作。一个人死于肺的空隙的纤维变性。不同年龄方括号的等级数据显示早易传染的复杂并发症有操作和控制组之间的明显的差别。与控制组相比,有的操作组受益例如更低的极限的更少复杂并发症,特别易传染的复杂并发症,和发生静脉的血栓,和结果显示出著名差别。结论: 在有 intertrochanteric 骨折的 Evans III 类型的老病人,肺的感染的发生最高。在更低的极限的静脉的血栓是最严肃的复杂并发症。执行四头肌 femories 功能锻练将因此是有用的。手术后的效果也与操作途径被联系。 | Chun Zhang Xijing He Binshang Lan Haopeng Li | 2007 | Journal of Nanjing Medical University2007，21，5： | 4 |
| 3 | Tuning charge density of chimeric antigen receptor optimizes tonic signaling and CAR-T cell fitness显示文摘Tonic signaling of chimeric antigen receptor(CAR),i.e.,the spontaneous CAR activation in the absence of tumor antigen stimulation,is considered to be a pivotal event controlling CAR-T efficacy.However,the molecular mechanism underlying the spontaneous CAR signals remains elusive.Here,we unveil that positively charged patches(PCPs)on the surface of the CAR antigenbinding domain mediate CAR clustering and result in CAR tonic signaling.For CARs with high tonic signaling(e.g.,GD2.CAR and CSPG4.CAR),reducing PCPs on CARs or boosting ionic strength in the culture medium during ex vivo CAR-T cell expansion minimizes spontaneous CAR activation and alleviates CAR-T cell exhaustion.In contrast,introducing PCPs into the CAR with weak tonic signaling,such as CD19.CAR,results in improved in vivo persistence and superior antitumor function.These results demonstrate that CAR tonic signaling is induced and maintained by PCP-mediated CAR clustering.Notably,the mutations we generated to alter the PCPs maintain the antigen-binding affinity and specificity of the CAR.Therefore,our findings suggest that the rational tuning of PCPs to optimize tonic signaling and in vivo fitness of CAR-T cells is a promising design strategy for the nextgeneration CAR. | Jian Chen Shizhen Qiu Wentao Li Kun Wang Yu Zhang Han Yang Baichuan Liu Guangfei Li Li Li Min Chen Junjie Lan Jiahua Niu Peijie He Lei Cheng Gaofeng Fan Xin Liu Xianmin Song Chenqi Xu Haitao Wu Haopeng Wang | 2023 | Cell Research2023，33，5： | 2 |
| 4 | Olfactory ensheathing cell transplantation for spinal cord injury An 18-year bibliometric analysis based on the Web of Science显示文摘OBJECTIVE:Olfactory ensheathing cell (OEC) transplantation is a promising new approach for the treatment of spinal cord injury (SCI),and an increasing number of scientific publications are devoted to this treatment strategy.This bibliometric analysis was conducted to assess global research trends in OEC transplantation for SCI.DATA SOURCE:All of the data in this study originate from the Web of Science maintained by the Institute for Scientific Information,USA,and includes SCI-EXPANDED,SSCI,A&HCI,CPCI-S,CPCI-SSH,BKCI-S,BKCI-SSH,CCR-EXPANDED and IC.The Institute for Scientific Information's Web of Science was searched using the keywords 'olfactory ensheathing cells' or 'OECs' or 'olfactory ensheathing glia' or 'OEG' or 'olfactory ensheathing glial cells' or 'OEGs' and 'spinal cord injury' or 'SCI' or 'spinal injury' or 'spinal transection' for literature published from January 1898 to May 2012.DATA SELECTION:Original articles,reviews,proceedings papers and meeting abstracts,book chapters and editorial materials on OEC transplantation for SCI were included.Simultaneously,unpublished literature and literature for which manual information retrieval was required were excluded.MAIN OUTCOME MEASURES:All selected literatures addressing OEC transplantation for SCI were evaluated in the following aspects:publication year,document type,language,author,institution,times cited,Web of Science category,core source title,countries/territories and funding agency.RESULTS:In the Web of Science published by the Institute for Scientific Information,the earliest literature record was in April,1995.Four hundred and fourteen publications addressing OEC transplantation for SCI were added to the data library in the past 18 years,with an annually increasing trend.Of 415 records,405 publications were in English.Two hundred and fifty-nine articles ranked first in the distribution of document type,followed by 141 reviews.Thirty articles and 20 reviews,cited more than 55 times by the date the publication data were downloaded by us,can be regarded as the most classical references.The journal Experimental Neurology published the most literature (32 records),followed by Glia.The United States had the most literature,followed by China.In addition,Yale University was the most productive institution in the world,while The Second Military Medical University contributed the most in China.The journal Experimental Neurology published the most OEC transplantation literature in the United States,while Neural Regeneration Research published the most in China.CONCLUSION:This analysis provides insight into the current state and trends in OEC transplantation for SCI research.Furthermore,we anticipate that this analysis will help encourage international cooperation and teamwork on OEC transplantation for SCI to facilitate the development of more effective treatments for SCI. | Zikuan Leng Xijing He Haopeng Li Dong Wang Kai Cao | 2013 | Neural Regeneration Research2013，8，14： | 2 |
| 5 | Synthesis, self- assembly and photoindueed surface-relief gratings of a polyaerylate-based Azo polyeleetrolyte显示文摘 | Yaning He Haopeng Wang Xinlin Luo | 2004 | Optical Materials2004，26，： | 1 |
| 6 | Synthesis,self-assembly and photoinduced surface-relief gratings of a polyacrylate-based Azo polyelectrolyte显示文摘 | HE Yaning WANG Haopeng LUO Xinlin | 2004 | Optical Materials2004，26，： | 1 |
| 7 | Proliferation and differentiation of reactive nestin^+/GFAP^+ cells in an adult rat model of compression-induced spinal cord injury显示文摘BACKGROUND:Studies have demonstrated that astrocytes may possess similar properties to neural stem cells/neural precursor cells and have the potential to differentiate into neurons. OBJECTIVE:To observe neuroepithelial stem cell protein(nestin) and glial fibrillary acidic protein (GFAP) expression following spinal cord injury,and to explore whether nestin^+/GFAP^+ cells,which are detected at peak levels in gray and white matter around the ependymal region of the central canal in injured spinal cord,possess similar properties of neural stem cells. DESIGN,TIME AND SETTING:A randomized,controlled experiment.The study was performed at the Key Laboratory of Environment and Genes Related to Diseases(Xi'an Jiaotong University), Ministry of Education between January 2004 and December 2006. MATERIALS:Rabbit anti-rat nestin,β-tubulinⅢ,mouse anti-rat GFAP,galactocerebroside(GaLC) antibodies were utilized,as well as flow cytometry. METHODS:A total of 60 male,Sprague Dawley rats,aged 8 weeks,were randomly assigned to control(n = 12) and model(n = 48) groups.The spinal cord injury model was established in the model group by aneurysm clip compression,while the control animals were not treated.The gray and white matter around the ependymal region of the central canal exhibited peak expression of nestin^+/GFAP^+ cells.These cells were harvested and prepared into single cell suspension,followed by primary and passage cultures.The cells were incubated with serum-containing neural stem cell complete medium. MAIN OUTCOME MEASURES:Nestin and GFAP expression in injured spinal cord was determined using immunohistochemistry and double-labeled immunofluorescence at 1,3,5,7,14,28,and 56 days post-injury.In addition,cell proliferation and differentiation were detected using immunofluorescence cytochemistry and flow cytometry. RESULTS:Compared with the control group,the model group exhibited significantly increased nestin and GFAP expression(P<0.05),which reached peak levels between 3 and 7 days.The majority of cells in the ependymal region around the central canal were nestin^+/GFAP^- cells,while the gray and white matter around the ependymal region were full of nestin^+/GFAP^+ cells,with an astrocytic-like appearance.A large number of nestin^+/GFAP^+ cells were observed in the model group cell culture,and the cells formed clonal spheres and displayed strong nestin-positive immunofluorescence staining.Following induced differentiation,a large number of GaLC-nestin,β-tubulinⅢ-nestin,and GFAP-nestin positive cells were observed.However,no obvious changes were seen in the control group.Cells in S stage,as well as the percentage of proliferating cells,in the model group were significantly greater than in the control group(P<0.01). CONCLUSION:Spinal cord injury in the adult rat induced high expression of nestin^+/GFAP^+ in the gray and white matter around the ependymal region of the central canal.These nestin^+/GFAP^+ cells displayed the potential to self-renew and differentiate into various cells.The cells could be neural stem cells of the central nervous system. | Pinglin Yang Xijing He Haopeng Li Binshang Lan Guoyu Wang Yiheng Liu | 2009 | Neural Regeneration Research2009，4，10： | 1 |
| 8 | p75 neurotrophin receptor signal pathway influence on apoptosis in anterior horn neurons of the spinal cord in a rat model of cauda equina compression injury显示文摘BACKGROUND: Studies have demonstrated that cauda equina compression results in apoptosis ofmotor neurons in the spinal cord. The combination of p75 neurotrophin receptor (p75NTR) andprecursor of nerve growth factor (pro-NGF) expression initiates the apoptotic pathway and inducesneuronal apoptosis. However, few reports have focused on the p75-mediated mechanism ofneuronal apoptosis following cauda equine compression injuryOBJECTIVE: To determine apoptosis of spinal cord neurons and activation of thepro-NGF-p75NTR-JNK(c-Jun N-terminal kinase) signal pathway in rats following cauda equinacompression, and to verify experimental outcomes.DESIGN, TIME AND SETTING: A randomized, controlled, in vivo experiment was performed at theMedical Experimental Center of Xi'an Jiaotong University between April and November in 2008.MATERIALS: Streptavidin-perosidase kit was purchased from Wuhan Boster, China; in situ endlabeling detection kit was provided by Promega, USA; type AEG-220G electron microscope waspurchased from Hitachi, Japan.METHODS: A total of 48 healthy, adult, female, Sprague Dawley rats were randomly assigned tothree groups: normal (n = 6), sham-surgery (n = 6), and compression (n = 36). The compressiongroup was randomly assigned to six subsets at 1,3, 5, 7, 14, and 28 days, respectively, with 6 rats ineach subset. A cylindrical silica gel stick was implanted into the rats to compress 75% of thevertebral canal in the compression group; in the sham-surgery group, only vertebral resection wasperformed; and no procedures were performed in the normal group.MAIN OUTCOME MEASURES: At 1,3, 5, 7, 14, and 28 days following compression, L_(2-3) spinalcord segments were processed for immunohistochemistry, in situ cell apoptosis detection, andtransmission electron microscopy observation. Nissl staining was used to observe neuronal survivalin the L_2 spinal cord segment. Immunohistochemistry was applied to detect expressions of pro-NGF,p75NTR, and JNK in the L_2 segment. TUNEL fluorometric method was used to observe apoptosis ofneurons in the L_2 segment.RESULTS: In the normal and sham-surgery groups, little neuronal apoptosis was observed in theL_(2-3) spinal cord segment. At 3 days after compression injury, pro-NGF, p75NTR and JNK expressionwas observed in the spinal cord. Expression levels reached a peak at 7 days, and then graduallydecreased. In the compression and sham-surgery groups, neurons primarily expressed pro-NGFand p75NTR. The number of JNK-positive neurons in the compression group was dramaticallyincreased compared with the sham-surgery group (P < 0.05). A few neurons were apoptotic in thespinal cord 1 day after compression injury. The number of apoptotic neurons gradually increasedand reached a peak at 7 days, and subsequently decreased. Apoptosis was still detectable at 28days. There was a positive correlation between p75NTR expression and neuronal apoptosis(r= 0.75, P< 0.05).CONCLUSION: Following cauda equina compression injury, apoptosis of spinal cord neurons wasobserved. The compression-induced neuronal apoptosis was associated with p75NTR expression inthe L_(2-3) spinal cord segment. | Haopeng Li Jie Chen Xijing He Siyue Xu Yu Liu | 2009 | Neural Regeneration Research2009，4，12： | 1 |
| 9 | Semaphorin 3A expression in spinal cord injured rats after olfactory ensheathing cell transplantation显示文摘Semaphorin 3A expression is thought to increase following spinal cord injury.The impact of olfactory ensheathing cell transplantation remains unclear.The current study demonstrated that spinal cord hemorrhage,edema,degeneration,necrosis,cyst formation,proliferation of glial cells,regeneration of nerve fibers and various pathological reactions occurred following a simple cross-section of spinal cord injury.Transplantation of olfactory ensheathing cells was found to significantly relieve the pathological reactions in the spinal cord described above,decrease the extent of necrosis in damaged neurons and nerve fibers,and downregulate semaphorin 3A expression in the injured zone.The results confirmed that olfactory ensheathing cell transplantation plays a protective role on the injured spinal cord by reducing the expression of semaphorin 3A. | Guoyu Wang Xijing He Puwei Yuan Haopeng Li Rui Chang | 2011 | Neural Regeneration Research2011，6，10： | 0 |
| 10 | Population genomic analysis reveals distinct demographics and recent adaptation in the black flying fox(Pteropus alecto)显示文摘As the only mammalian group capable of powered flight,bats have many unique biological traits.Previous comparative genomic studies in bats have focused on long-term evolution.However,the microevolutionary processes driving recent evolution are largely under-explored.Using resequencing data from 50 black flying foxes(Pteropus alecto),one of the model species for bats,we find that black flying fox has much higher genetic diversity and lower levels of linkage disequilibrium than most of the mammalian species.Demographic inference reveals strong population fluctuations(>100 fold)coinciding with multiple historical events including the last glacial change and Toba super eruption,suggesting that the black flying fox is a very resilient species with strong recovery abilities.While long-term adaptation in the black flying fox is enriched in metabolic genes,recent adaptation in the black flying fox has a unique landscape where recently selected genes are not strongly enriched in any functional category.The demographic history and mode of adaptation suggest that black flying fox might be a well-adapted species with strong evolutionary resilience.Taken together,this study unravels a vibrant landscape of recent evolution for the black flying fox and sheds light on several unique evolutionary processes for bats comparing to other mammalian groups. | Haopeng He Hechuan Yang Randy Foo Wharton Chan Feng Zhu Yunsong Liu Xuming Zhou Liang Ma Lin-Fa Wang Weiwei Zhai | 2023 | Journal of Genetics and Genomics2023，50，8： | 0 |
| 11 | Review of advanced road materials, structures, equipment, and detection technologies显示文摘As a vital and integral component of transportation infrastructure,pavement has a direct and tangible impact on socio-economic sustainability.In recent years,an influx of groundbreaking and state-of-the-art materials,structures,equipment,and detection technologies related to road engineering have continually and progressively emerged,reshaping the landscape of pavement systems.There is a pressing and growing need for a timely summarization of the current research status and a clear identification of future research directions in these advanced and evolving technologies.Therefore,Journal of Road Engineering has undertaken the significant initiative of introducing a comprehensive review paper with the overarching theme of“advanced road materials,structures,equipment,and detection technologies”.This extensive and insightful review meticulously gathers and synthesizes research findings from 39 distinguished scholars,all of whom are affiliated with 19 renowned universities or research institutions specializing in the diverse and multidimensional field of highway engineering.It covers the current state and anticipates future development directions in the four major and interconnected domains of road engineering:advanced road materials,advanced road structures and performance evaluation,advanced road construction equipment and technology,and advanced road detection and assessment technologies. | JRE Editorial Office Maria Chiara Cavalli De Chen Qian Chen Yu Chen Augusto Cannone Falchetto Mingjing Fang Hairong Gu Zhenqiang Han Zijian He Jing Hu Yue Huang Wei Jiang Xuan Li Chaochao Liu Pengfei Liu Quantao Liu Guoyang Lu Yuan Ma Lily Poulikakos Jinsong Qian Aimin Sha Liyan Shan Zheng Tong B.Shane Underwood Chao Wang Chaohui Wang Di Wang Haopeng Wang Xuebin Wang Chengwei Xing Xinxin Xu Min Ye Huanan Yu Huayang Yu Zhe Zeng You Zhan Fan Zhang Henglong Zhang Wenfeng Zhu | 2023 | Journal of Road Engineering2023，3，4： | 0 |
| 12 | Comprehensive landscape of resistance mechanisms for neoadjuvant therapy in esophageal squamous cell carcinoma by single-cell transcriptomics显示文摘Dear Editor,Preoperative neoadjuvant therapy combined with surgical resection is the standard treatment for locally advanced esopha-geal squamous cell carcinoma(ESCC).However,more than half of patients having a partial response to neoadjuvant therapy,which is considered as a therapy-resistant phenotype and the mechan-ism is still unclear.The heterogeneity of the ESCC with surgery alone therapy were characterized by single-cell RNA sequencing(scRNA-seq)previously,but few report was about ESCC patients with neoadjuvant therapy.1 It is emergent to illustrate the comprehensive hallmarks of neoadjuvant therapy-resistance in ESCC at single cell level. | Yushang Yang Yanguo Li Haopeng Yu Zhenyu Ding Longqi Chen Xiaoxi Zeng Shunmin He Qi Liao Yi Zhao Yong Yuan | 2023 | Signal Transduction and Targeted Therapy2023，8，9： | 0 |
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