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| 1 | The Chinese Hα Solar Explorer(CHASE) mission: An overview显示文摘The Chinese Hα Solar Explorer(CHASE), dubbed “Xihe”—Goddess of the Sun, was launched on October 14, 2021 as the first solar space mission of China National Space Administration(CNSA). The CHASE mission is designed to test a newly developed satellite platform and to acquire the spectroscopic observations in the Hα waveband. The Hα Imaging Spectrograph(HIS)is the scientific payload of the CHASE satellite. It consists of two observational modes: raster scanning mode and continuum imaging mode. The raster scanning mode obtains full-Sun or region-of-interest spectral images from 6559.7 to 6565.9 ? and from 6567.8 to 6570.6 ? with 0.024 ? pixel spectral resolution and 1 min temporal resolution. The continuum imaging mode obtains photospheric images in continuum around 6689 ? with the full width at half maximum of 13.4 ?. The CHASE mission will advance our understanding of the dynamics of solar activity in the photosphere and chromosphere. In this paper, we present an overview of the CHASE mission including the scientific objectives, HIS instrument overview, data calibration flow, and first results of on-orbit observations. | Chuan Li Cheng Fang Zhen Li MingDe Ding PengFei Chen Ye Qiu Wei You Yuan Yuan MinJie An HongJiang Tao XianSheng Li Zhe Chen Qiang Liu Gui Mei Liang Yang Wei Zhang WeiQiang Cheng JianXin Chen ChangYa Chen Qiang Gu QingLong Huang MingXing Liu ChengShan Han HongWei Xin ChangZheng Chen YiWei Ni WenBo Wang ShiHao Rao HaiTang Li Xi Lu Wei Wang Jun Lin YiXian Jiang LingJie Meng Jian Zhao | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，8： | 7 |
| 2 | A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants显示文摘microRNAs (miRNAs)are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression,leading to gene silencing.We previously developed short tandem target mimic (STTM)technology to deactivate endogenous miRNAs in Arabidopsis.Here,we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis,tomato,rice,and maize,providing a resource for the functional interrogation of miRNAs.We not only revealed the functions of several miRNAs in plant development,but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and development.RNA-seq and small RNAseq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation,secondary metabolism,and ion-channel activity-associated electrophysiology,demonstrating that STTM technology is an effective approach for studying miRNA functions.To facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks,we have established an online Genome Browser (http://gffzzc750bc0108fa4044s6uuwn9bvxk606pcc.ffgz.tsg.suse.edu.cn/designindex2.php) to display the transcriptomic and miRNAomic changes in STTMinduced miRNA knockdown plants. | Ting Peng Mengmeng Qiao Haiping Liu Sachin Teotia Zhanhui Zhang Yafan Zhao Bobo Wang Dongjie Zhao Lina Shi Cui Zhang Brandon Le Kestrel Rogers Chathura Gunasekara Haitang Duan Yiyou Gu Lei Tian Jinfu Nie Jian Qi Fanrong Meng Lan Huang Qinghui Chen Zhenlin Wang Jinshan Tang Xiaoqing Tang Ting Lan Xuemei Chen Hairong Wei Quanzhi Zhao Guiliang Tang | 2018 | Molecular Plant2018，11，11： | 7 |
| 3 | Interaction between PCC Filler and Cellulosic Fiber in Fiber/Filler Co-refining System显示文摘Mineral fillers are important for conserving raw fiber materials and reducing production costs in the paper industry.However,the increase in filler content will inevitably result in strength reduction,which limits the adding amount of filler in paper production.In this study,we designed a cellulose fiber/filler co-refining approach to improve the strength and optical properties of paper;moreover,the synergistic interaction between fibers and precipitated calcium carbonate(PCC)fillers in the co-refining process was investigated.Results of fiber separation and PCC particle size analysis showed that,compared with conventional refining,the content of fines increased,whereas the PCC particle size decreased.More importantly,composites were formed between the PCC and fines,which promoted strength improvement of paper.Physical tests show that the tensile index of paper with 15%PCC content increased by 22%compared with that of the paper filled by conventional method,whereas the brightness and opacity of paper improved by fiber/filler co-refining for a specified filler content.These findings provide a basis for the further development of co-refining filling technology. | Sheng Qiang Shunxi Song Peiyao Wang Yujie Shi Linhao Li Haitang Liu | 2021 | Paper And Biomaterials2021，6，4： | 5 |
| 4 | Preparation of monoclonal antibody based indirect competitive ELISA for detecting 19-nortestosterone residue显示文摘19-Nortestosterone (NT) has been illegally used in horse racing to boost physical performance, and in animal husbandry to accelerate weight gain. To monitor the abuse of NT, our goal was to develop a commercial enzyme linked immunosorbent assay (ELISA) kit. For this purpose, hybridomas were prepared by fusing NS0 mouse myeloma cells with splenocytes isolated from immunized BALB/c mouse. Noncompetitive and competitive indirect ELISA were used to screen positive cell clones. To optimize the indirect competitive ELISA (icELISA) method, various methanol concentrations in assay buffer were evaluated. Matrix effects in urine and spiking test were also investigated. Finally, five hybridoma cell lines named NT-1, NT-2, NT-3, NT-4 and NT-5 were screened out. The corresponding monoclonal antibodies (mAbs) were of the IgG 1 isotype with a k light chain, and the antibody affinity of all mAbs were between 2.6×10 9 and 4.7×10 9 L/mol. The titer and IC 50 values of purified ascites were in the range of 0.64×10 5 2.56×10 5 and 0.55-1.0 ng/mL, respectively. Based on the NT-1 hybridoma, a heterologous icELISA method was developed for the quantitative detection of NT in cattle urine. The dynamic range was from 0.004 to 85.8 ng/mL, with a detection limit for the assay and IC 50 values of 0.002 and 0.55 ng/mL, respectively. Except for a high cross-reactivity (62%) to α-NT, negligible cross-reactivity to other compounds was observed. After optimization, 10% of methanol was used in the assay buffer, and a 20-fold dilution in cattle urine gave an inhibition curve almost the same as that in phosphate buffered saline. The correlation coefficient between the established icELISA and LC-MS/MS method was 0.9871. The results showed that the established heterologous icELISA method provides an excellent alternative for the detection of NT residues in food producing animals. | JIANG JinQing ZHANG HaiTang FAN GuoYing MA JinYou WANG ZiLiang WANG JianHua | 2011 | Chinese Science Bulletin2011，56，25： | 4 |
| 5 | Production and Characterisation of Monoclonal Antibodies against 19-Nortestosterone显示文摘Objective To produce anti‐19‐Nortestosterone (NT) monoclonal antibodies and identify their immunological characteristics. Methods Hybridomas were prepared by fusing NS0 mouse myeloma cells with splenocytes isolated from immunized BALB/c mice. Noncompetitive and competitive indirect ELISA were employed to screen positive cell clones. A caprylic acid ammonium sulphate (CAAP) method was used to purify NT mAb, and the Batty saturation method was used to determine the affinity constant (Kaff). Results Five hybridoma cell lines, named NT‐1, NT‐2, NT‐3, NT‐4, and NT‐5, were identified and their corresponding mAbs were of the IgG 1 isotype with a k light chain. The Kaffs of all mAbs were between 2.6 and 4.7×10 9 L/mol. The titers and IC 50 values of purified ascite fluids were in the range of (0.64-2.56)×10 5 and (0.55-1.0) ng/mL, respectively. Of all the cross‐reacting steroids, α‐NT was the most reactive with the mAbs at 62% with NT‐1 mAb and 64% with NT‐2 mAb. Negligible cross‐reactivity (<0.01%) with other steroids was observed. Conclusion The establishment of these hybridomas allows the potential development of a rapid test kit, and may provide an alternative method for the detection of NT residues in food producing animals. | JIANQ JinQing ZHANG HaiTang YAO SiXin WANG ZiLiang WANG JianHua | 2011 | Biomedical and Environmental Sciences2011，24，2： | 2 |
| 6 | Multiresidue determination of sarafloxacin, difloxacin, noritoxacin, and pefloxacin in fish using an enzyme-linkod immunosorbent assay显示文摘 | JIANG Jingqing ZHANG Haitang WANG Ziliang | 2011 | Procodia Environmental Sciences2011，8，： | 1 |
| 7 | Ilaprazole for the treatment of duodenal ulcer: a randomized, double-blind and controlled phase III trial显示文摘 | Ling Wang Liya Zhou Haitang Hu Sanren Lin Jielai Xia | 2012 | Current Medical Research & Opinion2012，，1： | 1 |
| 8 | A novel TIR-NBS-LRR gene regulates immune response to Phytophthora root rot in soybean显示文摘Phytophthora root rot(PRR),caused by Phytophthora sojae,is a devastating disease of soybean.The NBSLRR gene family is a class of plant genes involved in disease resistance.miRNA mediates plant response to biotic stresses by regulating the expression of target genes at the transcriptional or post-translational level.Glyma.16G135500,encoding an NBS-LRR-type protein,is a target of gma-miR1510 that responds to pathogen infections.We cloned and overexpressed Glyma.16G135500(naming it GmTNL16)and knocked down mi R1510 using short tandem target mimic technology to identify the roles of the GmTNL16/gma-mi R1510 pair in the interaction of soybean and the oomycete.By overexpressing GmTNL16 in transgenic hairy roots of soybean,we showed that biomass of P.sojae was lower in overexpressing hairy roots than in control roots.Thus,miR1510 expression was reduced upon P.sojae infection,reflecting the induced expression of GmTNL16 conferring resistance to P.sojae in soybean.Differentially expressed genes were enriched in plant-pathogen interaction,plant hormone signal transduction,and secondary metabolism by RNA sequencing analyze.In particular,jasmonate and salicylic acid pathway-associated genes,including JAZ,COI1,TGA,and PR,responded to P.sojae infection.All of these results indicate that the GmTNL16/gma-miR1510 pair participates in soybean defense response via the JA and SA pathways. | Li Zhou Sushuang Deng Huidong Xuan Xingxing Fan Ruidong Sun Jinming Zhao Haitang Wang Na Guo Han Xing | 2022 | The Crop Journal2022，10，6： | 1 |
| 9 | A New PPI, Ilaprazole Compared With Omeprazole in the Treatment of Duodenal Ulcer: A Randomized Double-Blind Multicenter Trial显示文摘 | Ling Wang Liya Zhou Sanren Lin Haitang Hu Jielai Xia | 2011 | Journal of Clinical Gastroenterology2011，，4： | 1 |
| 10 | Quantitative determination of helicid in rat plasma by liquid chromatography electrospray ionization mass spectrometry and its application to preliminary pharmacokinetic studies 显示文摘 | Jia Yuanwei Wang Guangji Xie Haitang | 2007 | Chromatography B2007，847，2： | 1 |
| 11 | Pharmaeoki- netic study of triptolide, a constituent of immunosuppressive Chi- nese herb medicine, in rats显示文摘 | SHAO Feng WANG Guangji XIE Haitang | 2007 | Biol Pharm Bull2007，30，4： | 1 |
| 12 | Threshold of Soil pH for Occurrence of Tobacco Bacterial Wilt in Henan Province显示文摘[Objective]The paper was to study the relationship between spatial distribution characteristics of soil p H and bacterial wilt in tobacco-growing area in Henan Province.[Method]The 21 tobacco-growing fields infected by bacterial wilt and 91 tobacco-growing fields with no cases were selected from Henan Province,and the pH of fields was determined.[Result]Bacterial wilt mainly occurred in the tobacco-growing fields in Luoshan,Pingqiao,Queshan,Biyang,Suiping,and Xiping,and the soil pH mainly ranged from 5.5 to 6.5(accounted for 84.75%of total surveyed tobacco fields);bacterial wilt had not been found in tobacco-growing fields in Tanghe,Zhengyang,Shangcai,Sheqi,Wuyang and Chenqu,and the soil pH mainly ranged from 6.5 to 7.0(accounted for 81.25%of total surveyed tobacco fields).There were significant differences in soil pH between the fields with and without bacterial wilt,and soil pH 6.5 could be regarded as the threshold for the occurrence of tobacco bacterial wilt in Henan Province.There was extremely significantly positive linear correlation between soil p H and latitude(y=0.7375 x-17.799,R2=0.386,P<0.01).The geographical boundary of soil pH 6.5 was roughly 33°N,and bacterial wilt was less likely to occur in the north tobacco-growing areas of the boundary.[Conclusion]The study will provide a scientific basis for the prevention and control of tobacco bacterial wilt in Henan. | Haitao Wang Haohao Hao Xiaojing Xu Haitang Huang Xueli Song Lijun Yang | 2021 | Plant Diseases and Pests2021，12，2： | 1 |
| 13 | , Quantitative determination of helicid in rat plasma by liquid chromatography-electrospray ionization mass spectrometry and its application to preliminary phannacokinetic studies显示文摘 | Yuanwei Jia Guangji Wang Haitang Xie | 2007 | Journal of Chromatography B2007，847，2： | 1 |
| 14 | Simultaneous rapid quantification of ginsenoside Rg1 and its secondary glycoside Rh1 and aglycone protopanaxatriol in rat plasma by liquid chromatography-mass spectrometry after solid-phase extraction显示文摘 | Sun J Wang G Haitang X Hao L Guoyu P Tucker I | 2005 | J Pharm Biomed Anal2005，38，： | 1 |
| 15 | Simultaneous rapid quantification of ginsenoside Rg1 and its secondary glycoside Rh1 and aglycone protopanaxatriol in rat plasma by liquid chromatography-mass spectrometry after solid-phase extraction 显示文摘 | Sun J Wang G Haitang X | 2005 | J Pharm Biomed Anal2005，38，1： | 1 |
| 16 | Production of fur- fural from waste aqueous hemicellulose solution of hardwood over ZSM-5 zeolite显示文摘 | Gao Hongling Liu Haitang Wang Haisong | 2014 | Bioresource Technology2014，172，： | 1 |
| 17 | Simultaneous rapid quantification of ginsenoside Rg1 and its secondary glycoside Rh1 and aglycone protopanaxatriol in rat plasma by liquid chromatography-mass spectrometry after solid-phase extraction显示文摘 | SUN J WANG G HAITANG X | 2005 | J Pharm Biomed Anal2005，38，1： | 1 |
| 18 | Uptake and me- tabolism of ginsenoside Rh2 and its aglycon protopanaxadiol by Caco-2 cells显示文摘 | Xie Haitang Wang Gaang ii Chen Miao | 2005 | Biol Pharm Bull2005，28，2： | 1 |
| 19 | Liquid chromatographic/mass spectrometry assay of triptolide in dog plasma and its application to pharmacokinetic study显示文摘 | Feng Shao Guangji Wang Jianguo Sun Haitang Xie | 2006 | Journal of Pharmaceutical and Biomedical Analysis2006，41，： | 1 |
| 20 | Preparation of artificial antigen and immunological trait for aflatoxin BI显示文摘 | ZHANG Haitang WANG Zilang | 2012 | Procedia Engineering2012，29，1： | 1 |
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