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| 1 | Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase显示文摘 | Sun, Yongwei Zhang, Xin Wu, Chuanyin He, Yubing Ma, Youzhi Hou, Han Guo, Xiuping Du, Wenming Zhao, Yunde Xia, Lanqin | 2016 | Molecular Plant2016，9，4： | 94 |
| 2 | A novel method for purification of recombinant adeno-associated virus vectors on a large scale显示文摘A novel method for recombinant adeno-associ-ated virus (rAAV) purification on large scale is described. The method involves three steps, including chloroform treatment, PEG/NaCl precipitation and chloroform extraction. The whole procedure can be performed in four hours. Using this purification method, we can reproducibly obtain, from 4 × 109 of proviral cells cultured in roller bottles, purified rAAV-GFP stocks with titers of around 5×1013 particles/mL and purity greater than 95%. The infectious titers of the vector stocks were up to 2×1012 TU/mL, thus particle-to-infectivity rate was about 25. Under an electronic microscope, most rAAV particles appeared full and a few were in intermediate form. Empty particles were rarely seen. The purified rAAV-GFP stocks have been successfully used in in vitro and in vivo transfection experiments. Therefore, this new method offers a simple, rapid and cost-effective way for large-scale rAAV purification. | WU Xiaobing DONG Xiaoyan WU Zhijian CAO Hui NIU Dongbin QU Jianguo WANG Hong HOU Yunde | 2001 | Chinese Science Bulletin2001，46，6： | 21 |
| 3 | Allelic Analyses of the Arabidopsis YUC1 Locus Reveal Residues and Domains Essential for the Functions of YUC Family of Flavin Monooxygenases显示文摘Flavin monooxygenases(FMOs) play critical roles in plant growth and development by synthesizing auxin and other signaling molecules.However,the structure and function relationship within plant FMOs is not understood.Here we defined the important residues and domains of the Arabidopsis YUC1 FMO,a key enzyme in auxin biosynthesis.We previously showed that simultaneous inactivation of YUC1 and its homologue YUC4 caused severe defects in vascular and floral development.We mutagenized the yuc4 mutant and screened for mutants with phenotypes similar to those of yuc1 yuc4 double mutants.Among the isolated mutants,five of them contained mutations in the YUC1 gene.Interestingly,the mutations identified in the new yuc1 alleles were concentrated in the two GXGXXG motifs that are highly conserved among the plant FMOs.One such motif presumably binds to flavin adenine dinucleotide(FAD) cofactor and the other binds to nicotinamide adenine dinucleotide phosphate(NADPH).We also identified the Ser139 to Phe conversion in yuc1,a mutation that is located between the two nucleotide-binding sites.By analyzing a series of yuc1 mutants,we identified key residues and motifs essential for the functions of YUC1 FMO. | Xianhui Hou Sainan Liu Florencia Pierri Xinhua Dai Li-Jia Qu Yunde Zhao | 2011 | Journal of Integrative Plant Biology2011，53，1： | 4 |
| 4 | The hepatitis B vaccine originally jointly developed by China and the US is safe and effective显示文摘According to the disease control and prevention data of China,hepatitis B vaccinations have caused 188 coincidence deaths of newborns totally from 2000 to 2013.No problems have been found with the vaccines.Besides inspection of the manufacturers,all vaccines are launched to the market after inspection of the central government of China.Similar events happened also in other vaccines.The recent deaths related to hepatitis B vaccination have been proved to be coincidence.The hepatitis B virus vaccine used is originally jointly developed by the Chinese government and the US government.Its safety has been proved by the inoculation of one billion people around the globe. | HOU YunDe | 2014 | Science China(Life Sciences)2014，57，5： | 2 |
| 5 | Complete DNA sequence and gene analysis of the virulence plasmid pCP301 of Shigella flexneri 2a显示文摘 | Jiyu Zhang Hong Liu Xiaobing Zhang Jian Yang Fan Yang Guowei Yang Yan Shen Yunde Hou Qi Jin | 2003 | Science in China Series C: Life Sciences2003，，5： | 1 |
| 6 | Effect of Radix Astragali seu Heclys- ari on the interferon system显示文摘 | Hou yunde Hu Yuewn | 1981 | Chinese Med J1981，94，： | 1 |
| 7 | A randomized controlled trial of low-dose recombinant human interferons α-2b nasal spray to prevent acute viral respiratory infections in military recruits显示文摘 | LuLu Gao Shouyi Yu Qing Chen Zhaojun Duan Jie Zhou Chen Mao Dexian Yu Wenchang Zhu Jun Nie Yunde Hou | 2010 | Vaccine2010，，28： | 1 |
| 8 | Genomic organization of EDSV strain AA-2显示文摘The genomic organization of egg drop syndrome virus strain AA-2 isolated in China is reported here. The genome was found to be 32 838 bp in length, approximately 2 kb shorter than those of the human subgenus C adenoviruses. Analysis of open reading frames indicated that the genes for major viral structural proteins (55 K, Penton base, pⅦ, pⅩ, pⅥ, Hexon, 100 K, pⅧ and Fiber), as well as EP, DNA polymerase and ⅣaⅡ are present in the expected locations in the genome. EDSV possesses no identifiable E3 and E4 regions and most proteins encoded by E3 and E4 regions of other adenoviruses genome were not presented in EDSV. | Liyu Zeng Qi Jin Jinggang Zhang Maoxiang Li Hong Li Lei Zhu Zhen Yin Yunde Hou | 1998 | Chinese Science Bulletin1998，43，7： | 0 |
| 9 | MOPCS:next-generation nucleic acid molecular biosensor显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has had a huge impact on the world,causing millions of deaths and a serious blow to the global economy.SARS-CoV-2 can aack most body tissues with devastating consequences^([1]). | Quan Liu Shang Chen Li Wang Zhaojun Duan Fuquan Xie Guojun Zhao Yunde Hou Dixian Luo | 2022 | National Science Review2022，9，9： | 0 |
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