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| 1 | 新致病基因在颅缝早闭Crouzon综合征中的初步机制研究显示文摘目的研究Rbp4(Retinol binding protein 4)、Gpc3(Glypican family of growth factor binding protein 3)、C1qtnf3(Collagenous repeat-containing sequence of 26 KDa protein)等新致病基因在颅缝早闭症中的发病机制,为疾病非手术治疗奠定理论基础。方法以Crouzon综合征Fgfr2cC342Y/+小鼠为实验模型,应用MicroCT和组织学染色,研究小鼠颅缝闭合模式;以Fgfr2cC342Y/+模型,RT-qPCR研究Rbp4、Gpc3、C1qtnf3等新致病基因的表达差异,初步探讨其在颅缝闭合过程中的调控作用;以体外培养的Fgfr2cC342Y/+小鼠颅缝细胞为模型,研究基因突变动物细胞增殖与代谢改变。结果获得Fgfr2cC342Y/+小鼠后额缝、冠状缝、人字缝、矢状缝等颅缝闭合模式,随颅骨发育、颅缝闭合,OC(Osteocalcin)、ALP(Alkalinephosphatase)表达增加,目的基因Rbp4、Gpc3、C1qtnf3表达下降,Msx2(Muscle segment homeobox gene 2)表达增加,杂合子与野生型小鼠之间均存在显著统计学差异,与前期人颅缝组织Microarray研究结果一致。Gpc1(Glypican family ofgrowth factor binding protein 1)、FliI(Flightless I)在颅缝闭合中的表达较恒定,野生型与杂合子之间未见明显统计学差异。体外培养Fgfr2cC342Y/+小鼠颅缝细胞,CellTiter96 MTS和Quant-iT Picogreen dsDNA细胞增殖与代谢分析结果显示,Fgfr2功能获得性突变可促进冠状缝细胞的增殖,从而导致成骨增加,颅缝早闭。结论 Fgfr2cC342Y/+模型新致病基因表达趋势与前期人颅缝组织Microarray研究结果一致,Rbp4、Gpc3、C1qtnf3可能在颅缝早闭中具重要调控作用。 | 杨娴娴 Jodie T Hatfield Susan J Hinze 穆雄铮 Peter J Anderson Barry C Powell | 2012 | 组织工程与重建外科杂志2012，8，5： | 3 |
| 2 | Estimating absorbed photosynthetically active radiation and leaf area index from spectral reflectance in wheat显示文摘 | ASRAR G M FUCHS E T KANEMASU J HATFIELD L | 1984 | Agronomy Journal1984，76，： | 1 |
| 3 | Intercepted photosynthetically active radiation in wheat canopies estimated by spectral reflectance 显示文摘 | Hatfield J L Asrar G Kanemasu E T | 1984 | Remote Sensing of Environment1984，14，123： | 1 |
| 4 | Phytochrome A overex- pression in transgenic tobacco: Correlation of dwarf phenotype with high concentrations of phytochrome in vascular tissue and attenuated gibberellin levels 显示文摘 | Jordan E T Hatfield P M Hondred D | 1995 | Plant Physiol1995，107，： | 1 |
| 5 | Tat-Neutralizing Antibodies in Vaccinated Macaques显示文摘 | Tikhonov I Ruckwardt T J Hatfield G S | 2003 | J Virol2003，77，5： | 1 |
| 6 | Emotional contagion显示文摘 | Hatfield E Cacioppo J T Rapson R L | 1993 | Current Directions in Psychological Science1993，2，3： | 1 |
| 7 | Evalution of cotton canopy temperature to detect crop water stress显示文摘 | Howell T A Hatfield J L Yamada H | 1984 | Trans ASAE1984，27，： | 1 |
| 8 | Norepinephrine infused into the basolateral amygdale posttraining enhances retention in a spatial water maze task显示文摘 | HATFIELD T MCGAUGH J L | 1999 | Neurobiol Learn Mem1999，71，2： | 1 |
| 9 | Intercepted photosynthetically active radiation in wheat canopies estimated by spectral reflectance显示文摘 | Hatfield J L Asrar G Kanemasu E T | 1984 | Re-mote Sensing of Environment1984，14，： | 1 |
| 10 | Surface energy balance of a corn residue-covered field显示文摘 | Sauer T J Hatfield J L Prueger J H | 1998 | Agricultural and Forest Meteorology1998，89，34： | 1 |
| 11 | Electroencephalographic studies of skilled psychomotor performance 显示文摘 | HATFIELD B D HAUFLER A J HUNG T M | 2004 | J Clinical Neurophysiol2004，21，3： | 1 |
| 12 | Crop Condition and Yield Simulations Using Landsat and MODIS显示文摘 | Hatfield J L Jackson T J | 2004 | Remote Sensing of Environment2004，92，： | 1 |
| 13 | Managing soils to achieve greater water use efficiency显示文摘 | Hatfield J L Sauer T J Prueger J H | 2001 | Agronomy Journal2001，93，3： | 1 |
| 14 | Intercepted photosynthetically active radiation in wheat canopies estimated by spectral reflectance显示文摘 | Hatfield J L Asrar G Kanemasu E T | 1984 | Remote Sensing of Environment1984，14，： | 1 |
| 15 | Crop condition and yield simulations using LANDSAT and MODIS显示文摘 | Doraiswamy P C Hatfield J L Jackson T J | 2004 | Remoto Sens- ing of Enviroment2004，92，4： | 1 |
| 16 | Synchronous autotransfusion during cesarean hysterectomy 显示文摘 | Hatfield T Kraus H McConnell D | 2010 | Am J Obstet Gynecol2010，202，1： | 1 |
| 17 | Norepinephrine infused into the basolateral amygdala posttraining enhances retention in a spatial water maze task显示文摘 | Hatfield T McGaugh J L | 1999 | Neurobiol Learn Mern1999，71，： | 1 |
| 18 | Electroencephalographic studies of skilled psychomotor performance 显示文摘 | Hatfield B D Haufler A J Hung T M | 2004 | Journal of Clinical Neurophysiology2004，21，3： | 1 |
| 19 | Crop condition and yield simulations using Landsat and MODIS显示文摘 | Doraiswamy P C Hatfield J L Jackson T J | 2004 | Remote Sensing of Environment2004，，92： | 1 |
| 20 | Amygdala norepinephrine levels after training predict inhibitory avoidance retention performance in rats显示文摘 | McIntyre CK Hatfield T McGaugh JL | 2002 | Eur J Neurosci2002，16，7： | 1 |
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