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| 1 | Three-dimensional bioprinting of gelatin methacryloyl (GelMA)显示文摘The three-dimensional (3D)bioprinting technology has progressed tremendously over the past decade.By controlling the size, shape,and architecture of the bioprinted constructs,3D bioprinting allows for the fabrication of tissue/organ-like constructs with strong structural-functional similarity with their in vivo counterparts at high fidelity.The bioink,a blend of biomaterials and living cells possessing both high biocompatibility and printability,is a critical component of bioprinting.In particular, gelatin methacryloyl (GelMA)has shown its potential as a viable bioink material due to its suitable biocompatibility and readily tunable physicochemical properties.Current GelMA-based bioinks and relevant bioprinting strategies for GelMA bioprinting are briefly reviewed. | Guoliang Ying Nan Jiang Cunjiang Yu Yu Shrike Zhang | 2018 | Bio-Design and Manufacturing2018，1，4： | 8 |
| 2 | Electrochemical Lithium Storage Performance of Molten Salt Derived V_(2)SnC MAX Phase显示文摘MAX phases are gaining attention as precursors of two-dimensional MXenes that are intensively pursued in applications for electrochemical energy storage.Here,we report the preparation of V_(2)SnC MAX phase by the molten salt method.V_(2)SnC is investigated as a lithium storage anode,showing a high gravimetric capacity of 490 mAh g−1 and volumetric capacity of 570 mAh cm^(−3) as well as superior rate performance of 95 mAh g^(−1)(110 mAh cm^(−3))at 50 C,surpassing the ever-reported performance of MAX phase anodes.Sup-ported by operando X-ray diffraction and density functional theory,a charge storage mechanism with dual redox reaction is proposed with a Sn-Li(de)alloying reaction that occurs at the edge sites of V_(2)SnC particles where Sn atoms are exposed to the electrolyte followed by a redox reaction that occurs at V_(2)C layers with Li.This study offers promise of using MAX phases with M-site and A-site elements that are redox active as high-rate lithium storage materials. | Youbing Li Guoliang Ma Hui Shao Peng Xiao Jun Lu Jin Xu Jinrong Hou Ke Chen Xiao Zhang Mian Li Per OÅPersson Lars Hultman Per Eklund Shiyu Du Zhifang Chai Zhengren Huang Na Jin Jiwei Ma Ying Liu Zifeng Lin Qing Huang | 2021 | Nano-Micro Letters2021，13，10： | 2 |
| 3 | Cobalt-containing covalent organic frameworks for visible light-driven hydrogen evolution显示文摘Covalent organic frameworks(COFs) have recently emerged as a new class of photocatalysts.However,integrated design is crucial to maximizing the performance of COF-incorporating photocatalytic systems.Herein,we compare two strategies of installing earth-abundant metal-based catalytic centers into the matrice of a 2 D COF named NUS-55.Compared to NUS-55(Co)prepared from the post-synthetic metalation of coordination sites within the COF,the molecular co-catalyst impregnated NUS-55/[Co(bpy)3]Cl2 achieves a seven-fold improvement in visible light-driven H2 evolution rate to 2,480 μmol g^-1h^-1,with an apparent quantum efficiency(AQE) of 1.55% at 450 nm.Our results show that the rational design of molecular anchoring sites in COFs for the introduction of catalytic metal sites can be a viable strategy for the development of highly efficient photocatalysts with enhanced stability and photocatalytic activities. | Jian Wang Jian Zhang Shing Bo Peh Guoliang Liu Tanay Kundu Jinqiao Dong Yunpan Ying Yuhong Qian Dan Zhao | 2020 | Science China Chemistry2020，63，2： | 2 |
| 4 | Construction of recombinant plasmid pEGFP-C2-L539fs/47 and its expression in HEK293 cells显示文摘Objective:To reconstruct pEGFP-C2-L539fs/47,a HERG nonsense mutant in eukaryotic expression plasmid,and observe the fusion protein expressed in HEK293 cells(human embryo kidney cells).Methods:After double digestion of pcDNA3-L539fs/47 and pEGFP-C2-HERG with sbf I and Eco91 I,the small product fragment,from pcDNA3-L539fs/47,was subcloned into the big fragment of pEGFP-C2-HERG under T4 ligase.pEGFP-C2-L539fs/47 was identified by agarose gel electrophoresis and sequencing.pcDNA3-L539fs/47 and pEGFP-C2-L539fs/47 were transiently transfected into HEK293 cells by Lipofect,respectively.The expression of fusion protein in HEK293 cells was detected through immunofluorescence,laser confocal imaging scanning in vivo,Western blot and PCR.Results:Mutation region cDNA fragment(about 1 kb) and target vector fragment(about 7.2 kb) were ligated after purification and gel recovery.Agarose gel electrophoresis and sequencing successfully demonstrated eukaryotic expression plasmid pEGFP-C2-L539fs/47,constructed approximately 8.2 kb,sequencing consistent with template gene.The transfection efficiency of recombinant plasmid by fluorescence microscopy was more than60%.Western blot analysis detected pcDNA3-L539fs/47 expression of the protein size 60 KD,the expression of pEGFP-C2 fusion protein size of approximately 90 KD.The L539fs/47 gene expression in HEK293 cells was significant by PCR analysis.Confocal laser imaging showed that pEGFP-C2-L539fs/47 protein was successfully expressed in cytoplasm and cytomembrane of HEK293 cells.Conclusion:pEGFP-C2-L539fs/47 containing the HERG gene mutant was successfully constructed by double digestion method and expressed fusion protein in HEK293 cells,which laid a foundation for the further study on L539fs/47. | Lue Ying Zhang Aifeng Han Wenqi Li Guoliang Zhang Junbo Gao Jie Pan Junqiang Zhang Yong Sun Chaofeng | 2012 | Journal of Medical Colleges of PLA(China)2012，27，3： | 2 |
| 5 | Experimental realization to efficiently sort vector beams by polarization topological charge via Pancharatnam–Berry phase modulation显示文摘This paper reports the experimental realization of efficiently sorting vector beams by polarization topological charge(PTC). The PTC of a vector beam can be defined as the repetition number of polarization state change along the azimuthal axis, while its sign stands for the rotating direction of the polarization. Here, a couple of liquid crystal Pancharatnam–Berry optical elements(PBOEs) have been used to introduce conjugated spatial phase modulations for two orthogonal circular polarization states. Applying these PBOEs in a 4-f optical system,our experiments show the setup can work for PTC sorting with a separation efficiency of more than 58%. This work provides an effective way to decode information from different PTCs, which may be interesting in many fields, especially in optical communication. | SHUIQIN ZHENG YING LI QINGGANG LIN XUANKE ZENG GUOLIANG ZHENG YI CAI ZHENKUAN CHEN SHIXIANG XU DIANYUAN FAN | 2018 | Photonics Research2018，6，5： | 2 |
| 6 | Generation and characterisation of rabbit monoclonal antibodies against the native cell surface antigens of embryonic stem cells显示文摘Embryonic stem (ES) cells are potent resources for cell therapy,and monoclonal antibodies (mAbs) against native cell surface markers of ES cells could be useful tools for therapeutic applications.Here,we report the development of a feasible approach,which could be used in mass production,for experimentally producing rabbit mAbs against native cell surface antigens on the cell surface.Two of the 14 mAbs,which were selected at random,could be bound to the cell surface antigens of mES cells.The immunocytochemistry (ICC) and Western blot results showed that mAb 39 recognises conformational epitopes.The target antigen of mAb 39 was then successfully purified using an improved immunoprecipitation approach in which mAb was bounded to intact mES cells before the cells were lysed.The LC-LTQ mass spectrum analysis showed that the target antigen of mAb 39 was Glut3.This result was further confirmed by Western blot using commercially available antibodies against Glut3.Further experiments showed that mAb 39 exhibited an antiproliferative effect on mES cells.We also found that Glut3 was differentially expressed among the mES cell population as detected by flow cytometry. | Yongliang Lu Zhongyuan Su Ying Li Jingfeng Luo Zhou Tan Huijiao Ji Weimin Zhu Guoliang Yu Liangbiao Chen Ming Zhang | 2010 | Journal of Genetics and Genomics2010，37，7： | 1 |
| 7 | The development of Bi Fe O_3-based ceramics显示文摘The multiferroic properties of Bi Fe O3-based ceramics were improved through optimizing their sintering method and doping with certain rare earth elements in pure Bi Fe O3. Some methods, especially liquid-phase sintering method has largely decreased the densities of oxygen vacancies and Fe2in Bi Fe O3-based ceramics, and thus their resistivity became high enough to measure the saturated polarization and the large piezoelectric d33 coefficient under the high electric field of [150 k V/cm. Besides,multiferroic properties were improved through the rare earth elements' doping in pure Bi Fe O3. Magnetization commonly increases with the proportional increase of Nd,La, Sm and Dy contents up to *30 %, while ferroelectric phase can transform to paraelectric phase at a certain proportion. An improved magnetoelectric coupling was often observed at ferroelectric phase with a relatively large proportion. Besides, an enhanced piezoelectric coefficient is expected in Bi Fe O3-based ceramics with morphotropic phase boundaries as they are already observed in thin epitaxial Bi Fe O3 films. | Ahmad Hussain Xijun Xu Guoliang Yuan Yiping Wang Ying Yang Jiang Yin Junming Liu Zhiguo Liu | 2014 | Chinese Science Bulletin2014，59，36： | 1 |
| 8 | Ghrelin inhibits cell apoptosis induced by lipotoxicity in pancreatic β-cell line显示文摘 | Wei Wang Dan Zhang Hong Zhao Ying Chen Ying Liu Cuiping Cao Lingling Han Guoliang Liu | 2010 | Regulatory Peptides2010，，1： | 1 |
| 9 | Quantitative analysis of diclofenac sodium powder via near-infrared spectroscopy combined with artifcial neural network 显示文摘 | Wang Bin Liu Guoliang Dou Ying | 2009 | Pharmaceutical and Biomedical Analysis2009，50，2： | 1 |
| 10 | Valuation and countermeasure of Diabetic Osteoporosis 显示文摘 | Guoliang H Ying Z | 2006 | Journal of Practical Diabetology2006，2，4： | 1 |
| 11 | Minimum Power Configuration for Wireless Com- munication in Sensor Networks 显示文摘 | Xing Guoliang Lu Chenyang Zhang Ying | 2007 | ACM Transactions on Sensor Networks2007，3，2： | 1 |
| 12 | Serum carbohydrate antigen-125 is elevated in patients with chronic atrial fibrillation显示文摘Objective: To determine whether CA-125 is elevated in medically stable patients with chronic atrial fibrillation (AF) compared with patients without AF and to examine whether levels of CA-125 are associated with demographic and clinical variables in a sample of patients under study. Methods: 55 patients with chronic AF and 58 control patients in sinus rhythm were included into the study. Patients with acute heart failure (HF), chronic inflammatory or neoplastic disease were excluded from the study. Circulating levels of CA-125 were assessed; all patients underwent clinical examination, assessment and medical records including demographic data, history of comorbid conditions, current use of cardiac medications, and the results of cardiac tests including electrocardiograms. Results: The mean age of the study sample was 53.2±6.5 years and 48% were men. Patients with sinus rhythm were significantly more likely to have lower heart rates, smaller dimensions of left atrium, and to be treated with aspirin. Coumadin, coumadin and digoxin were more often prescribed in patients with chronic AF. The CA-125 levels were significantly higher in patients with chronic AF than in patients in sinus rhythm (48.5±7.65 U/ml and 28.43±5.48 U/ml, P<0.005). An inverse relation was found between CA-125 levels and left ventricular ejection fraction (LVEF) (r=-0.48, P<0.001). CA-125 was significantly related to the left atrium (LA) diameter, left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD) and brain natriuretic peptide (BNP). There was no significant correlation between CA-125 and age. Conclusion: In subjects with chronic AF, CA-125 levels are increased; CA-125 was significantly related to the LA diameter, LVEDD, LVESD and BNP. | Pan Junqiang Zhang Dianxin Zhang Zaiwei Lu Ying Zhou Xin Li Guoliang Sun Chaofeng | 2012 | Journal of Medical Colleges of PLA(China)2012，27，5： | 1 |
| 13 | Simulation on the Port Timing of an Air-powered Engine显示文摘 | Chen Ying Liu Hao Tao Guoliang | 2005 | International Journal of Vehicle Design2005，38，： | 1 |
| 14 | Kinetic study on the preparation of 4,4'-dinitrostilbene-2,2'-disulfonic acid(I)-kinetic study on the oxidation of 4,4'-dinitro-bibenzyl-2,2'-disulfonicacid to prepare 4,4'-dintrostilbene-2,2'-disulfonic acid显示文摘 | Liu Ying Zhang Fengbao Zhang Guoliang | 2003 | Dyes and Pigments2003，56，3： | 1 |
| 15 | Asymmetric epigenome maps of subgenomes reveal imbalanced transcription and distinct evolutionary trends in Brassica napus显示文摘The complexity of the epigenome landscape and transcriptional regulation is significantly increased during plant polyploidization,which drives genome evolution and contributes to the increased adaptability to diverse environments.However,a comprehensive epigenome map of Brassica napus is still unavailable.In this study,we performed integrative analysis of five histone modifications,RNA polymerase Ⅱ CCU-pancy,DNA methylation,and transcriptomes in two B.napus lines(2063A and B409),and established global maps of regulatory elements,chromatin states,and their dynamics for the whole genome(including the An and Cn subgenomes)in four tissue types(young leaf,flower bud,silique,and root)of these two lines.Approximately 65.8% of the genome was annotated with different epigenomic signals.Compared with the Cn subgenome,the An subgenome possesses a higher level of active epigenetic marks and lower level of repressive epigenetic marks.Genes from subgenome-unique regions contribute to the major differences between the An and Cn subgenomes.Asymmetric histone modifications between homeologous gene pairs reflect their biased expression patterns.We identified a novel bivalent chromatin state(with H3K4me1 and H3K27me3)in B.napus that is associated with tissue-specific gene expression.Furthermore,we observed that different types of duplicated genes have discrepant patterns of histone modification and DNA methylation levels.Collectively,our findings provide a valuable epigenetic resource for allopolyploid plants. | Qing Zhang Pengpeng Guan Lun Zhao Meng Ma Liang Xie Yue Li Ruiqin Zheng Weizhi Ouyang Shunyao Wang Hongmeijuan Li Ying Zhang Yong Peng Zhilin Cao Wei Zhang Qin Xiao Yuanling Xiao Tingdong Fu Guoliang Li Xingwang Li Jinxiong Shen | 2021 | Molecular Plant2021，14，4： | 1 |
| 16 | Geology and geochemistry of the Changba SEDEX Pb-Zn deposit, Qinling orogenic belt, China显示文摘 | Guoliang Ma Georges Beaudoin Sijing Qi Ying Li | 2004 | Mineralium Deposita2004，，3： | 1 |
| 17 | Induction of CD4+CD25+Foxp3+ regulatory T cell response by glatiramer acetate in type 1 diabetes显示文摘Glatiramer 醋酸盐(GA ) 是过去常对待多重硬化的 immunomodulatory 肽药。它的处理效果被扩展了到象 uveoretinitis,煽动性的肠疾病,接枝拒绝和肝的纤维变性那样的另外的自体免疫的条件。这里,我们报导 GA 在在 cyclophosphamide (CY ) 改变糖尿病的临床的功课是有效的加强的非肥胖的糖尿病患者(CY 点头) 老鼠。有显著地减少的 GA 的治疗在老鼠和改善 insulitis 的糖尿病的率,它与增加的 CD4+CD25+Foxp3+ T 房间反应与一致在对待老鼠。GA 处理导致了抄写因素 Foxp3 的增加的表示并且在 vivo 并且在 vitro 提高了 interleukin-4 (IL-4 ) 的生产。Foxp3 的起来规定上的 GA 的效果通过 IL-4 部分被调停,是明显的。IL-4 被发现维持 Foxp3 表示和 CD4+CD25+ 规章的 T 房间(Tregs ) 的规章的功能。这研究提供 GA 通过 Tregs 的正式就职为类型 1 糖尿病有处理潜力,那增加的 IL-4 生产为提高的 Treg 在 GA 处理的功能部分负责的新证据。 | Guoliang Cui Yuebo Zhang Zhenwei Gong Jingwu Z Zhang Ying Qin Zang | 2009 | Cell Research2009，19，5： | 1 |
| 18 | Three-dimensional silk fibroin microsphere-nanofiber scaffolds for vascular tissue engineering显示文摘A significant limitation in the engineering of artificial small-diameter vascular scaffolds is that the number of endothelial cells(ECs)is not sufficient to generate a confluent coverage of the vascular scaffolds,so that the surfaces of vascular scaffolds form thrombus via platelet adhesion and aggregation.Thrombus decrease relies on three-dimensional(3D)scaffolds to mimic the natural extracellular matrix(ECM)as templates to regulate cell behavior and facilitate tissue maturation.Here,we developed 3D scaffolds consisting of silk fibroin(SF)nanofibers and homogeneous microspheres by electrospinning and microfluidics.The nanofibers with diameters ranging from 250 to 350 nm doped with microspheres(2–10μm)formed bridge-shaped structures.ECs were seeded and maintained on the 3D microsphere-nanofiber scaffolds with a mean fiber diameter of 300 nm.A 10%higher ratio of cell proliferation on 3D microsphere-nanofiber SF scaffolds was noted as compared to that on microporous and sponge-like SF scaffolds with small surface network fabricated by freeze-drying.Moreover,the gene transcript levels including CD146,VE-C and PECAM-1 were better preserved on 3D microsphere-nanofiber SF scaffolds than those on freeze-dried scaffolds.Thus,the developed 3D microsphere-nanofiber structure may have a myriad of applications in vascular tissue engineering scaffolds and cardiovascular devices. | Qiang Liu Guoliang Ying Nan Jiang Ali KYetisen Danyu Yao Xiaoying Xie Yubo Fan Haifeng Liu | 2021 | Medicine in Novel Technology and Devices2021，，1： | 0 |
| 19 | Recursive filtering of multi-rate cyber-physical systems with unknown inputs under adaptive event-triggered mechanisms显示文摘Cyber-physical systems(CPSs)take on the characteristics of both multiple rates of information collection and processing and the dependency on information exchanges.The purpose of this paper is to develop a joint recursive filtering scheme that estimates both unknown inputs and system states for multi-rate CPSs with unknown inputs.In cyberspace,the information transmission between the local joint filter and the sensors is governed by an adaptive event-triggered strategy.Furthermore,the desired parameters of joint filters are determined by a set of algebraic matrix equations in a recursive way,and a sufficient condition verifying the boundedness of filtering error covariance is found by resorting to some algebraic operation.A state fusion estimation scheme that uses local state estimation is proposed based on the covariance intersection(CI)based fusion conception.Lastly,an illustrative example demonstrates the effectiveness of the proposed adaptive event-triggered recursive filtering algorithm. | Ying SUN Miaomiao FU Jingyang MAO Guoliang WEI | 2024 | Frontiers of Information Technology & Electronic Engineering2024，25，2： | 0 |
| 20 | Inflammatory risk stratification individualizes anti-inflammatory pharmacotherapy for acute type A aortic dissection显示文摘The systemic benefits of anti-inflammatory pharmacotherapy vary across cardiovascular diseases in clinical practice.We aimed to evaluate the application of artificial intelligence to acute type A aortic dissection(ATAAD)patients to determine the optimal target population who would benefit from urinary trypsin inhibitor use(ulinastatin).Patient characteristics at admission in the Chinese multicenter 5A study database(2016-2022)were used to develop an inflammatory risk model to predict multiple organ dysfunction syndrome(MODS). | Hong Liu Haiyang Li Lu Han Yingyuan Zhang Ying Wu Liang Hong Jinong Yang Jisheng Zhong Yuqi Wang Dongkai Wu Guoliang Fan Junquan Chen Shengqiang Zhang Xingxing Peng Zhihua Zeng Zhiwei Tang Zhanjie Lu Lizhong Sun Sichong Qian Yongfeng Shao Hongjia Zhang on behalf of the Additive Anti-inflammatory Action for Aortopathy&Arteriopathy(A)Investigators | 2023 | The Innovation2023，4，4： | 0 |
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