|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Bombyx mori bidensovirus:The type species of the new genus Bidensovirus in the new family Bidnaviridae显示文摘Bombyx mori bidensovirus(BmBDV),which had been assigned to Densovirinae in Parvoviridae previously,replicates mainly in silkworm midgut columnar cells and causes the fatal flacheric disease.In contrast to parvovirus,this virus possesses two single-stranded DNA genome segments and encodes a putative protein-primed DNA polymerase.The accumulating evidence suggests that it has unique characteristics different to parvovirus and adopts its own mechanisms for replication.So far,little is known about the replication mechanisms of BmBDV.In this review,we focus on the pathology associated with this virus and the viral biology such as viral genome structure,viral genes,and viral replication and expression strategies. | HU ZhaoYang LI GuoHui LI GuangTian YAO Qin CHEN KePing | 2013 | Chinese Science Bulletin2013，58，36： | 6 |
| 3 | NEDD9 promotes cancer stemness by recruiting myeloid-derived suppressor cells via CXCL8 in esophageal squamous cell carcinoma显示文摘Objective:Esophageal squamous cell carcinoma(ESCC)has high morbidity and mortality rates worldwide.Cancer stem cells(CSCs)may cause tumor initiation,metastasis,and recurrence and are also responsible for chemotherapy and radiotherapy failures.Myeloid-derived suppressor cells(MDSCs),in contrast,are known to be involved in mediating immunosuppression.Here,we aimed to investigate the mechanisms of interaction of CSCs and MDSCs in the tumor microenvironment.Methods:ESCC tissues and cell lines were evaluated.Neural precursor cell expressed,developmentally downregulated 9(NEDD9)was knocked down and overexpressed by lentiviral transfection.Quantitative PCR,Western blot,immunohistochemistry,cell invasion,flow cytometry,cell sorting,multiplex chemokine profiling,and tumor growth analyses were performed.Results:Microarray analysis revealed 10 upregulated genes in esophageal CSCs.Only NEDD9 was upregulated in CSCs using the sphere-forming method.NEDD9 expression was correlated with tumor invasion(P=0.0218),differentiation(P=0.0153),and poor prognosis(P=0.0373).Additionally,NEDD9 was required to maintain the stem-like phenotype.Screening of chemokine expression in ESCC cells with NEDD9 overexpression and knockdown showed that NEDD9 regulated C-X-C motif chemokine ligand 8(CXCL8)expression via the ERK pathway.CXCL8 mediated the recruitment of MDSCs induced by NEDD9 in vitro and in vivo.MDSCs promoted the stemness of ESCC cells through NEDD9 via the Notch pathway.Conclusions:As a marker of ESCC,NEDD9 maintained the stemness of ESCC cells and regulated CXCL8 through the ERK pathway to recruit MDSCs into the tumor,suggesting NEDD9 as a therapeutic target and novel prognostic marker for ESCC. | Dongli Yue Shasha Liu Tengfei Zhang Yong Wang Guohui Qin Xinfeng Chen Huanyu Zhang Dong Wang Lan Huang Feng Wang Liping Wang Song Zhao Yi Zhang | 2021 | Cancer Biology & Medicine2021，18，3： | 4 |
| 4 | Medical Additive Manufacturing: From a Frontier Technology to the Research and Development of Products显示文摘1.Research and development(R&D)and the challenges of raw materials for medical additive manufacturing Raw materials for medical additive manufacturing have a wide range of commonalities that are also seen in many other fields,making them an important basis in the field of three-dimensional(3D)printing.Problems and challenges related to material types,powder properties,formability,viscoelasticity,and so forth also share common features.For example,many metal materials are used in the field of aviation,while metals,polymers,and inorganic materials are used in the field of biomedicine.The most widely used materials in biomedicine are biocompatible.Various homogeneous and non-homogeneous composites are also available for 3D printing,and impose an additional challenge in additive manufacturing;the use of heterogeneous composites in 3D printing is particularly challenging. | Guixing Qiu Wenjiang Ding Wei Tian Ling Qin Yu Zhao Lianmeng Zhang Jian Lu Daijie Chen Guangyi Yuan Chengtie Wu Bingheng Lu Ruxu Du Jimin Chen Mo Elbestawi Zhongwei Gu Dichen Li Wei Sun Yuanjin Zhao Jie He Dadi Jin Bin Liu Kai Zhang Jianmo Li Kam WLeong Dewei Zhao Dingjun Hao Yingfang Ao Xuliang Deng Huilin Yang ShaoKeh Hsu Yingqi Chen Long Li Jianping Fan Guohui Nie Yun Chen Hui Zeng Wei Chen Yuxiao Lai | 2020 | Engineering2020，6，11： | 1 |
| 5 | A porous C/LiFePO4/multiwalled carbon nanotubes cathode material for lithium ion batteries显示文摘 | Qin Guohui Ma Qianqian Wang Chengyang | 2014 | Electrochimica Acta2014，115，： | 1 |
| 6 | Determination of Major,Minor and Trace Elements in Seamount Phosphorite by XRF显示文摘 | WANG Xiaohong LI Guohui ZHANG Qin | 2004 | Geostandards and Geoanalytical Research2004，28，1： | 1 |
| 7 | Determination of Major,Minor and Trace Element,in Seamount Phosphorite by XRF显示文摘 | Wang Xiaohong Li Guohui Zhang Qin | 2004 | Geostandards and Geoanalytical Research2004，28，1： | 1 |
| 8 | Determination of major, minor and trace elements in seamount phosphorite by XRF 显示文摘 | Wang Xiaohong Li Guohui Zhang Qin Wang Yimin | 2004 | Gestand Geoanal Res2004，28，1： | 1 |
| 9 | Determination of major,minor and trace elements in seamount phosphorite by XRF显示文摘 | WANG Xiaohong LI Guohui ZHANG Qin WANG Yimin | 2004 | Gestand Geoanal Res2004，28，1： | 1 |
| 10 | Photocatalytic reduction of carbon dioxide to formic acid, formaldehyde, and methanol using dye-sensitized TiO2 film显示文摘 | Guohui Qin Yue Zhang Xuebin Ke | 2013 | Applied Catalysis B: Environmental2013，129，： | 1 |
| 11 | Myeloid cells in COVID-19 microenvironment显示文摘Varying differentiation of myeloid cells is common in tumors,inflammation,autoimmune diseases,and metabolic diseases.The release of cytokines from myeloid cells is an important driving factor that leads to severe COVID-19 cases and subsequent death.This review briefly summarizes the results of single-cell sequencing of peripheral blood,lung tissue,and cerebrospinal fluid of COVID-19 patients and describes the differentiation trajectory of myeloid cells in patients.Moreover,we describe the function and mechanism of abnormal differentiation of myeloid cells to promote disease progression.Targeting myeloid cell-derived cytokines or checkpoints is essential in developing a combined therapeutic strategy for patients with severe COVID-19. | Guohui Qin Shasha Liu Li Yang Weina Yu Yi Zhang | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 0 |
| 12 | Overcoming resistance to immunotherapy by targeting GPR84 in myeloid-derived suppressor cells显示文摘Myeloid-derived suppressor cells(MDSCs)were found to gradually accumulate in the orthotopic esophageal cancer mouse model during tumor progression.Although the roles of MDSCs in promoting tumor growth and inhibiting immune response have been extensively explored,currently,there are still no effective means for targeting MDSCs clinically.The deficiency of specific markers of MDSCs was responsible for the limited strategy to eliminating in clinic.This study identified that GPR84 was exclusively overexpressed on MDSCs.It was further found that GPR84 was prominently expressed on MDSCs in clinical samples and tumor mouse models,which drives the immunosuppression on CD8^(+)T cells by inhibiting PD-L1 degradation in lysosomes.Furthermore,G-CSF and GM-CSF were found to induce GPR84 expression through the STAT3/C/EBPβsignaling pathway.In addition,GPR84+MDSCs and PD-L1^(+)MDSCs were highly accumulated in anti-PD-1 therapy-resistant patients with esophageal cancer,and high GPR84 signature risk was verified as a negative factor for the overall survival of patients with anti-PD-1 treatment.Finally,GPR84 antagonism combined with an anti-PD-1 antibody enhanced the antitumor responses.Therefore,targeting GPR84 enhanced anti-PD-1 efficacy in esophageal cancer and other malignant tumors.This combination therapy has the potential for tumor therapy in clinics. | Guohui Qin Shasha Liu Jinyan Liu Hongwei Hu Li Yang Qitai Zhao Congcong Li Bin Zhang Yi Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，5： | 0 |
| 13 | Meta‑analysis of the Association between Serotonin Transporter Polymorphisms and Sudden Infant Death Syndrome显示文摘The serotonin transporter(5‑HTT)gene has been considered one of the risk factors for sudden infant death syndrome(SIDS),but the association remains unconfirmed.This meta‑analysis was performed to quantitatively summarize the evidence for such a relationship.PubMed,EMBASE,and China National Knowledge Infrastructure databases were searched for eligible studies within a range of published years from 1990 to December 2015.The odds ratios(ORs)with 95%confidence intervals(CIs)were used to assess the different associations.A total of 8 studies with 624 cases and 796 controls were included for 5‑HTT promoter polymorphism,5 studies with 418 cases and 542 controls for intron 2,and 3 studies with 253 cases and 334 controls for haplotype.The pooled examinations showed an overall increased SIDS risk for the 5‑HTT promoter polymorphism(OR=1.65,95%CI=1.03–2.63,P=0.035 for LL vs.LS and SS;OR=1.46,95%CI=1.04–2.04,P=0.028 for L vs.S),but no association(OR=1.00,95%CI=0.75–1.33,P=0.994 for 10+9 carriers vs.12/12;OR=0.97,95%CI=0.79–1.19,P=0.753 for 10+9 vs.12)for intron 2 polymorphism,and an unreliable association(OR=0.52,95%CI=0.31–0.87,P=0.013)for S‑9 and S‑10 haplotypes.This meta‑analysis suggests that the L allele or LL homozygote of 5‑HTT promoter polymorphism has an increased risk for SIDS,while intron 2 polymorphism has no association with SIDS. | Haojie Qin Guohui Xu Xinmin Pan Yaonan Mo | 2016 | Journal of Forensic Science and Medicine2016，2，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员