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| 1 | Telmisartan induces melanoma cell apoptosis and synergizes with vemurafenib in vitro by altering cell bioenergetics显示文摘Objective: Despite recent advancements in targeted therapy and immunotherapies, prognosis for metastatic melanoma patients remains extremely poor. Development of resistance to previously effective treatments presents a serious challenge and new approaches for melanoma treatment are urgently needed. The objective of this study was to examine the effects of telmisartan, an AGTR1 inhibitor and a partial agonist of PPARγ, on melanoma cells as a potential agent for repurposing in melanoma treatment.Methods: Expression of AGTR1 and PPARγ m RNA in melanoma patient tumor samples was examined in publicly available datasets and confirmed in melanoma cell lines by qRT-PCR. A panel of melanoma cell lines was tested in viability, apoptosis and metabolic assays in presence of telmisartan by flow cytometry and immunocytochemistry. A cytotoxic effect of combinations of telmisartan and targeted therapy vemurafenib was examined using the Chou-Talalay combination index method.Results: Both AGTR1 and PPARγ mRNA were expressed in melanoma patient tumor samples and decreased compared to the expression in the healthy skin. In vitro, we found that telmisartan decreased melanoma cell viability by inducing cell apoptosis.Increased glucose uptake, but not utilization, in the presence of telmisartan caused the fission of mitochondria and release of reactive oxygen species. Telmisartan altered the cell bioenergetics, thereby synergizing with vemurafenib in vitro, and even sensitized vemurafenib-resistant cells to the treatment.Conclusions: Given that the effective doses of telmisartan examined in our study can be administered to patients and that telmisartan is a widely used and safe antihypertensive drug, our findings provide the scientific rationale for testing its efficacy in treatment of melanoma progression. | Jelena Grahovac Tatjana Srdic-Rajic Juan Francisco Santibanez Marijana Pavlovic Milena Cavic Sinisa Radulovic1 | 2019 | Cancer Biology & Medicine2019，16，2： | 3 |
| 2 | Numerical algorithm for rigid body position estimation using the quaternion approach显示文摘This paper deals with rigid body attitude estimation on the basis of the data obtained from an inertial measurement unit mounted on the body. The aim of this work is to present the numerical algorithm, which can be easily applied to the wide class of problems concerning rigid body positioning, arising in aerospace and marine engineering, or in increasingly popular robotic systems and unmanned aerial vehicles. Following the considerations of kinematics of rigid bodies, the relations between accelerations of different points of the body are given. A rotation matrix is formed using the quaternion approach to avoid singularities. We present numerical procedures for determination of the absolute accelerations of the center of mass and of an arbitrary point of the body expressed in the inertial reference frame, as well as its attitude. An application of the algorithm to the example of a heavy symmetrical gyroscope is presented, where input data for the numerical procedure are obtained from the solution of differential equations of motion, instead of using sensor measurements. | Miodrag Zigic Nenad Grahovac | 2018 | Acta Mechanica Sinica2018，34，2： | 2 |
| 3 | 用于脂肪移植及脂肪干细胞分离获取与处理技术的对比显示文摘背景与目的脂肪获取和处理技术的差异可能会影响脂肪移植的成功率。本研究利用裸鼠模型,对比不同处理方法获取的脂肪移植物的性质,并评估移植脂肪组织中存活组织的体积。方法第一阶段,通过负压吸引辅助脂肪抽吸或超声辅助脂肪抽吸2种方式获取脂肪,并利用2种孔隙尺寸的筛网进行过滤。对移植材料的如下方面进行分析:平均团块大小,油脂、脂肪和水溶性成分的构成比,以及基质血管细胞群(stromalvascularfraction,SVF)的产量。将过滤物及滤液注入无胸腺的裸鼠皮下。第二阶段,将仅通过负压吸引吸脂术中获取脂肪抽吸物,进行离心、纱布摇匀或过滤处理,然后利用相似的规范进行研究。结果与负压吸引脂肪抽吸术相比,超声辅助的脂肪抽吸术获取的脂肪在SVF含量和脂肪移植体内研究成活量情况均相似。通过超声辅助的脂肪抽吸术仅比负压脂肪抽吸术产生稍微多的油脂;通过500μm或800μm孔径的滤网进行过滤均可有效去除液体和油脂。利用纱布法处理的脂肪,其体内移植成活率和SVF产量均最高。对所有移植物的组织学分析均显示为完整的脂肪组织。结论通过超声辅助及负压吸引行脂肪抽吸术获取的脂肪,其在异种移植模型中具有相似的成活率。对于少量填充移植而要求最佳成活率的身体敏感区域而言,利用纱布摇匀的处理方式可能是用于美容性脂肪移植的最佳方法。过滤法和离心法均可有效去除液体成分,其处理过的脂肪具有相似的移植成活率,当所需移植体积较大时,这2种方法均可行。 | Christine Fisher Tara L. Grahovac Mark E. Schafer Ron D. Shippert Kacey G. Marra J. Peter Rubin 魏峰 高景恒 张晨 | 2014 | 中国美容整形外科杂志2014，25，4： | 2 |
| 4 | Intracranial hemorrhage after stenting and angioplasty of extracranial carotid stenosis显示文摘 | Grahovac S Douen A | 2000 | AJNR Am J Neuroradiol2000，21，： | 1 |
| 5 | Nightmare complication after lumbar disc surgery:cranial nontraumatic acute epidural hematoma 显示文摘 | Grahovac G Vilendecic M Chudy D | 2011 | Spine ( Phila Pa 1976 )2011，36，26： | 1 |
| 6 | Sharing and Lateral Transshipment of Inventory in a Supply Chain with Expensive Low - demand Items 显示文摘 | Grahovac J Chakravarty A | 2001 | Management Science2001，47，4： | 1 |
| 7 | Renal fibrosis and glomerulosclerosis in a new mouse model of diabetic nephropathy and its regression by bone morphgenic priotein-7 and advanced glycation end product inhlbitors 显示文摘 | Hikaru Sugimoto Gordan Grahovac Michael Zeiberg | 2007 | Dibetetes2007，45，7： | 1 |
| 8 | Intracranial hem orrhage after stenting and angioplasty of extracranial ca rotid stenosis 显示文摘 | Morrish W Grahovac S Douen A | 2000 | AJNR Am J Neuroradiol2000，21，： | 1 |
| 9 | Learning, Imitation, and the Use of Knowledge: A Comparison of Markets, Hierarchies, and Teams显示文摘 | John C. Butler Jovan Grahovac | 2012 | Organization Science2012，，5： | 1 |
| 10 | Intracranial hemorrhage after stenting and angioplasty of extracranial carotid stenosis显示文摘 | Morrish W Grahovac S Douen A | 2000 | Am J Neuroradiol2000，21，： | 1 |
| 11 | Intracranial hemorrhage after stenting and angioplasty of extracranial carotid stenosis 显示文摘 | Morrish W Grahovac S Douen A | 2000 | AJNR Am J Neuroradiol2000，21，： | 1 |
| 12 | Sharing and lateral transshipment of in- ventory in a supply chain with expensive low-demand items显示文摘 | Grahovac J Chakravarty A | 2002 | Management Science2002，11，2： | 1 |
| 13 | Sharing and lateral transshipment of inventory in a supply chain with ex- pensive low-demand items 显示文摘 | GRAHOVAC J CHAKRAVARTY A | 2002 | Management Science2002，11，2： | 1 |
| 14 | Renal fibro-sis and glomerulosclerosis in a new mouse model of dia-betic nephropathy and its regression by bone morphogenicprotein-7 and advanced glycation end product inhibitors显示文摘 | Sugimoto H Grahovac G Zeisberg M | 2007 | D/^ere52007，56，7： | 1 |
| 15 | Effect of an annular closure de- vice(barricaid) on same level recurrent disc herniation and disc height loss after primary lumbar discectomy : two-year results of a multi-center prospective cohort study 显示文摘 | Parker SL Grahovac G Vukas D | 2013 | J Spinal Disord Tech2013，5，： | 1 |
| 16 | Charcot shoulder caused by Chiari type I malformation with syringomyelia with six-year follow-up显示文摘 | Grahovac G Vilendecic M Srdoc D | | 0，，15： | 1 |
| 17 | Cerebellar glio- blastomas: pathophysiology, clinical presentation and man- agement显示文摘 | Grahovac G Tomac D Lambasa S | 2009 | Acta Neuroehir2009，151，6： | 1 |
| 18 | Evalua- tion of a multi-layer adipose-derived stem cell sheet in a full-thickness wound healing model显示文摘 | LIN Y C GRAHOVAC T OH S J | 2013 | Acta Bio- mater2013，9，： | 1 |
| 19 | Evaluation of a multi-layer adiposederived stem cell sheet in a full-thickness wound healing model显示文摘 | Lin YC Grahovac T Oh SJ | 2013 | Acta Biomater2013，9，2： | 1 |
| 20 | Intracranial hemorrhage after stenting and angioplasty of extracranial carotid stenosis 显示文摘 | Morrish W Grahovac S Douen A | 2000 | AJNR Am J Neuroradiol2000，21，10： | 1 |
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