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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Hongda New Material Improved Silicone Rubber Industrial Chain显示文摘 | Jiangsu Hongda Chemical New Material Co.,Ltd.(Hongda New material,SZ:002211)is located in Yangzhong,Jiangsu province.It got listed in Shenzhen Stock Exchange onFebruary 1st,2008.(CCR2008, No.3)The company mainly produces silicone monomersand high-temperature silicone rubber series.The capacity of silicone monomers and prod-ucts of silicone rubber series reached 30 000 t/a and 28 000 t/a respectively at the end of2007.The sales volume of high-temperature silicone rubber compound has grown steadilyat 30% a year.The company’s high-temperature silicone rubber capacity is still beingexpanded today and will hopefully reach 50 000 t/a at the end of 2008. | 2008 | China Chemical Reporter2008，，25： | 16 |
| 3 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 4 | Viability and virulence of experimentally stressed noncuhurable Salmonella typhimurium 显示文摘 | Caro A Got P Lesne J Binard S | 1999 | Appl Environ Microbiol1999，65，7： | 1 |
| 5 | Biomechanical compari- son of parallel versus 90 - 90 plating of bicolumn distal hu-merus fractures with intra - articular comminution 显示文摘 | Got C Shuck J Biereevicz A | 2012 | J Hand Surg(Am)2012，37，12： | 1 |
| 6 | Biomechanical Comparision of parallel versus 90-90plating of bicolumn distal humerus fracture with intra-articular comminution显示文摘 | Got C Shuck J Biercevicz A | 2012 | J Hand Surg(Am)2012，37，12： | 1 |
| 7 | Prevalence of Act inobacillus pleuropneumoniae,Actinobacillus suis,Haemophiluspara suis,Pasteurella multocida,and Streptococcus suis in representative Ontario swine herds显示文摘 | MACINNES J GOT T SCHALK M LONE A G | 2008 | Can J Vet Res2008，72，3： | 1 |
| 8 | Viability and virulence ofexperimentally stressed nonculturable Salmonella typhimu-rium显示文摘 | CARO A GOT P LESNE J | 1999 | Appl Environ Microbiol1999，65，7： | 1 |
| 9 | Management of patients hospitalized for diabetic foot infection: results of the French OPIDIA study 显示文摘 | Richard JL Lavigne JP Got I Hartemann A | 2011 | Diabetes Metab2011，37，3： | 1 |
| 10 | Biomechanical comparison of parallel versus 90-90 plating of bicolumn distal humerus fractures with intra-articular comminution显示文摘 | Got C Shuck J Biercevicz A | | 0，，： | 1 |
| 11 | Value of intracardiac ultra-sound in diagnosis of arrthrthmogenic right ventricular dysplasia-cardiomyopathy显示文摘 | Peter S Brattstrom A Gotting B | | 0，，02： | 1 |
| 12 | Physicochemical properties of FK506, a novel immunosuppressant isolated from streptomyces tsukubaenis 显示文摘 | Kuroda A Marusawa H Hashimoto M Hatanaka H Kino T Got o T Okuhara M | 1987 | Transplant Proc1987，19，6： | 1 |
| 13 | Viability and virulence of experimentally stressed nonculturable Salmonella typhimurium显示文摘 | Caro A Got P Lesne J | | 0，，7： | 1 |
| 14 | Beta-galactosidase of Helix pomatia显示文摘 | Got R Marnay A Jarrige P | 1964 | Nature1964，204，： | 1 |
| 15 | Sedimentation on the Ionian ac- tive margin(Hellenieare) :Provenance of sediments and mecha- nisms of deposition 显示文摘 | Got H Monaco A Vittori J | 1981 | Sedimentary Geology1981，28，4： | 1 |
| 16 | Long - term follow - up evaluation of surgery for ossification of the posterior longitudinal ligament 显示文摘 | Got A Kita T | 1995 | Spine1995，20，20： | 1 |
| 17 | Biomechanical comparison of parallel versus 90-90 plating of bicolumn distal humerus fractures with intra-articular comminution显示文摘 | Got C Shuck J Biercevicz A | 2012 | J Hand Sttrg Am2012，37，12： | 1 |
| 18 | Numerical simulation of incremental forming of sheet metal 显示文摘 | Yamashit A M Got Oh M Atsumi S Y | 2008 | Journal of Materials Pro- cessing Technology2008，199，： | 1 |
| 19 | Experimental airborne transmission of Streptococcus suis capsular type 2 in pigs显示文摘 | Bert H F Got Schalk M Labbe A | 2001 | Vet Microbiol2001，82，： | 1 |
| 20 | Filter structures for equalization and diversity combining in wireless communications显示文摘 | Venkat~ S Mahmoud S A Got~ran R A | 1994 | The 3th annual international conference on universal personal communications1994，，： | 1 |
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