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| 1 | Effect of different exogenous fatty acids on the cytosolic triacylglycerol content in bovine mammary cells显示文摘The objective of this study was to determine how cytosolic triacylglycerols(TAG) are stored in mammary cells and whether this depends on the individual chemical configuration of fatty acids(FA). This objective was accomplished by addition of different FA to a FA-free medium used to culture mammary alveolar cells-large T antigen cells(MAC-T). Treatments consisted of adding FA(palmitate, stearate, oleate, linoleate,rumenic acid [CLA], elaidate and vaccinate) solutions to the medium at 100, 200, 300 and 400 mmol/L concentrations for a 24-h incubation period. At the end of each incubation period, cytosolic TAG, DNA and protein contents were measured. Palmitate, vaccenate, linoleate and CLA increased(P < 0.05) cytosolic TAG(μg/mg protein). Palmitate and CLA increased(P < 0.05) cytosolic TAG adjusted for DNA content. Overall, effects on cytosolic TAG accumulation depended on individual FA structure(chain length, degree of saturation, and number and orientation of FA double bonds). In addition, the long-chain FA used in this study did not have a detrimental effect on MAC-T cells as indicated by cytosolic protein and DNA contents reflecting their biological role in lipid accumulation. | Einar Vargas-Bello-Pérez Juan J.Loor Philip C Garnsworthy | 2019 | Animal Nutrition2019，，2： | 2 |
| 2 | Seasonal variation in milk conjugated linoleic acid and Δ 9 -desaturase activity in dairy cows显示文摘 | A.L Lock P.C Garnsworthy | 2002 | Livestock Production Science2002，，1： | 2 |
| 3 | Independent effect of dietary linoleic and linolenic fatty acids on the conjugated linoleic acid content of cows'milk显示文摘 | Lock A L Garnsworthy P C | 2002 | AnimalScience2002，74，： | 1 |
| 4 | Modelling responses to nutritional, endocrine and genetic strategies to increase fertility in the UK dairy herd显示文摘 | Maas J A Garnsworthy P C Flint A P F | 2009 | Veterinary Journal2009，180，3： | 1 |
| 5 | Short Communication: Effect of Production Variables on the Cis-9, Trans-11 Conjugated Linoleic Acid Content of Cows Milk 显示文摘 | LOCK D E BAUMAN D E and GARNSWORTHY P C | 2005 | J Dairy Sci2005，88，8： | 1 |
| 6 | Seasonal Variation in Milk Conjugated Linoleic Acid and 9-Desaturase Activity in Dairy Cows显示文摘 | LOCK A L GARNSWORTHY P C | 2003 | Livestock Production Science2003，79，： | 1 |
| 7 | Independent Effects of Dietary Linoleic and Linolenic Fatty Acids on the Conjugated Linoleic Acid Content of Cows' Milk显示文摘 | LOCK A L and GARNSWORTHY P C | 2002 | Anita Sci2002，74，： | 1 |
| 8 | The effect of body condition of dairy cows at calving on their food intake and performance when given complete diets显示文摘 | Garnsworthy P C Topps J H | 1982 | Anim Prod1982，35，： | 1 |
| 9 | Variation of milk citrate with stage of lacta-tion and de novo fatty acid synthesis in dairy cows显示文摘 | GARNSWORTHY P C MASSON L L LOCK AL | 2006 | J Dairy Sci2006，89，5： | 1 |
| 10 | Independent effects of dietary linoleic and linolenic fatty acids on the con- jugated linoleic acid content of cows' milk 显示文摘 | LOCK A GARNSWORTHY P C | 2002 | Ani- mal Science2002，74，： | 1 |
| 11 | The effect of supple- menting grass silage with barley on digestibility, in sacoo degrad- ability, rumen fermentation and methane production in sheep at two levels of intake 显示文摘 | Moss A R Givens D I Garnsworthy P C | 1995 | Animal Feed Science and Technology1995，50，12： | 1 |
| 12 | Seasonal variation in milk conjugated linoleic acid and △9 - desaturase activity in dairy cows 显示文摘 | Lock A L Garnsworthy P C | 2003 | Livestock Production Science2003，，79： | 1 |
| 13 | Independent effects of dietary linoleic and linolenic fatty acids on the conjugated linoleic acid content of cows' milk显示文摘 | LOCK A L GARNSWORTHY P C | 2002 | Anim Sci2002，74，： | 1 |
| 14 | Short communication: Effect of production variables on the cis-9, trans-ll conjugated linoleic acid content of cows' milk显示文摘 | LOCK A L BAUMAN D E GARNSWORTHY P C | 2005 | J Dairy Sci2005，88，8： | 1 |
| 15 | Identification of the physical stimulus and the neural basis of fabric : evoked 显示文摘 | Garnsworthy R K Gully R L Kenins P etal | 1988 | J Neurophysiol1988，59，： | 1 |
| 16 | Seasonal variation in milk conjugated linoleic acid and △9-desaturase activity in dairy cows显示文摘 | LOCK A L GARNSWORTHY P C | 2003 | Livest Prod Sci2003，79，： | 1 |
| 17 | Seasonal variation in milk conjugated linoleic acid and Delta(9)-desaturase activity in dairy cows 显示文摘 | Lock AL Garnsworthy PC | 2003 | Livestock Production Sci2003，79，1： | 1 |
| 18 | Transcutaneous electrical stimulation and the sensat ion of prickle 显示文摘 | Garnsworthy R K Gully R L Kenins P | 1988 | J Neurophysiol1988，59，： | 1 |
| 19 | Effect of synchronizing the rate of dietary energy and nitrogen release on rumen fermentation and microbial protein synthesis in sheep显示文摘 | Sinclair L A P C Garnsworthy J R Newbold | | 0，，： | 1 |
| 20 | Independent effect of dietary linoleic and linolenic fatty acids on the conjugated linoleic acid content of cow's milk显示文摘 | Lock A L Garnsworthy P C | 2002 | Animal Science2002，74，： | 1 |
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