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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | The influence of DC stray current on pipeline corrosion显示文摘DC stray current can cause severe corrosion on buried pipelines.In this study,firstly,we deduced the equation of DC stray current interference on pipelines.Next,the cathode boundary condition was discretized with pipe elements,and corresponding experiments were designed to validate the mathematical model.Finally,the numerical simulation program BEASY was used to study the corrosion effect of DC stray current that an auxiliary anode bed generated in an impressed current cathodic protection system.The effects of crossing angle,crossing distance,distance of the two pipelines,anode output current,depth,and soil resistivity were investigated.Our results indicate that pipeline crossing substantially affects the corrosion potential of both protected and unprotected pipelines.Pipeline crossing angles,crossing distances,and anode depths,our results suggest,have no significant influence.Decreasing anode output current or soil resistivity reduces pipeline corrosion gradually.A reduction of corrosion also occurs when the distance between two parallel pipelines increases. | Gan Cui Zi-Li Li Chao Yang Meng Wang | 2016 | Petroleum Science2016，13，1： | 18 |
| 3 | Advanced Space-based Solar Observatory(ASO-S):an overview显示文摘The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar community.The scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single platform.ASO-S is scheduled to be launched into a 720 km Sun-synchronous orbit in 2022.This paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C. | Wei-Qun Gan Cheng Zhu Yuan-Yong Deng Hui Li Yang Su Hai-Ying Zhang Bo Chen Zhe Zhang Jian Wu Lei Deng Yu Huang Jian-Feng Yang Ji-Jun Cui Jin Chang Chi Wang Ji Wu Zeng-Shan Yin Wen Chen Cheng Fang Yi-Hua Yan Jun Lin Wei-Ming Xiong Bin Chen Hai-Chao Bao Cai-Xia Cao Yan-Ping Bai Tao Wang Bing-Long Chen Xin-Yu Li Ye Zhang Li Feng Jiang-Tao Su Ying Li Wei Chen You-Ping Li Ying-Na Su Hai-Yan Wu Mei Gu Lei Huang Xue-Jun Tang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 17 |
| 4 | Statistical scenarios forecasting method for wind power ramp events using modified neural networks显示文摘Wind power ramp events increasingly affect the integration of wind power and cause more and more problems to the safety of power grid operation in recent years.Several forecasting techniques for wind power ramp events have been reported.In this paper,the statistical scenarios forecasting method is proposed for wind power ramp event probabilistic forecasting based on the probability generating model.Multi-objective fitness functions are established considering cumulative density functions and higher order moment autocorrelation functions with respect to the consistency of distribution and timing characteristics,respectively.Parameters of probability generating model are calculated by the iterative optimization using the modified genetic algorithm with multi-objective fitness functions.A number of statistical scenarios captured bands are generated accordingly.Eventually,ramp event probability characteristics are detected from scenarios captured bands to evaluate the ramp event forecasting method.A wind plant of Bonneville Power Administration with actual wind power data is selected for calculation and statistical analysis.It is shown that statistical results with multi-objective functions are more accurate than the results with single objective functions.Moreover,the statistical scenarios forecasting method can accurately estimate the characteristics of wind power ramp events.The results verify that the proposed method can guide the generation method of statistical scenarios and forecasting models for ramp events. | Mingjian CUI Deping KE Di GAN Yuanzhang SUN | 2015 | Journal of Modern Power Systems and Clean Energy2015，3，3： | 13 |
| 5 | An efficient methodology for utilization of K-feldspar and phosphogypsum with reduced energy consumption and CO2 emissions显示文摘The issues of reducing CO_2 emissions, sustainably utilizing natural mineral resources, and dealing with industrial waste offer challenges for sustainable development in energy and the environment. We propose an efficient methodology via the co-reaction of K-feldspar and phosphogypsum for the extraction of soluble potassium salts and recovery of SO_2 with reduced CO_2 emission and energy consumption. The results of characterization and reactivity evaluation indicated that the partial melting of K-feldspar and phosphogypsum in the hightemperature co-reaction significantly facilitated the reduction of phosphogypsum to SO_2 and the exchange of K^+(K-feldspar) with Ca^(2+)(CaSO_4 in phosphogypsum). The reaction parameters were systematically investigated with the highest sulfur recovery ratio of ~ 60% and K extraction ratio of ~ 87.7%. This novel methodology possesses an energy consumption reduction of ~ 28% and CO_2 emission reduction of ~ 55% comparing with the present typical commercial technologies for utilization of K-feldspar and the treatment of phosphogypsum. | Zhixi Gan Zheng Cui Hairong Yue Siyang Tang Changjun Liu Chun Li Bin Liang Heping Xie | 2016 | Chinese Journal of Chemical Engineering2016，24，11： | 11 |
| 6 | A de novo Genome of a Chinese Radish Cultivar显示文摘Here, we report a high-quality draft genome of a Chinese radish(Raphanus sativus) cultivar. This draft contains 387.73 Mb of assembled scaffolds, 83.93% of the scaffolds were anchored onto nine pseudochromosomes and 95.09% of 43 240 protein-coding genes were functionally annotated. 184.75 Mb(47.65%) of repeat sequences was identified in the assembled genome. By comparative analyses of the radish genome against 10 other plant genomes, 2 275 genes in 780 gene families were found unique to R. sativus. This genome is a good reference for genomic study and of great value for genetic improvement of radish. | ZHANG Xiaohui YUE Zhen MEI Shiyong QIU Yang YANG Xinhua CHEN Xiaohua CHENG Feng WU Zhangyan SUN Yuyan JING Yi LIU Bo SHEN Di WANG Haiping CUI Na DUAN Yundan WU Jian WANG Jinglei GAN Caixia WANG Jun WANG Xiaowu LI Xixiang | 2015 | Horticultural Plant Journal2015，1，3： | 10 |
| 7 | Achieving and maintaining biological nitrogen removal via nitrite under normal conditions显示文摘The principal aim of this paper is to develop an approach to realize stable biological nitrogen removal via nitrite under normal conditions. Validation of the new method was established on laboratory-scale experiments applying the sequencing batch reactor(SBR) activated sludge process to domestic wastewater with low C/N ratio. The addition of sodium chloride(NaCl) to influent was established to achieve nitrite build-up. The high nitrite accumulation, depending on the salinity in influent and the application duration of salt, was obtained in SBRs treating saline wastewater. The maintenance results indicated that the real-time SBRs can maintain stable nitrite accumulation, but conversion from shorter nitrification-denitrification to full nitrification-denitrification was observed after some operation cycles in the other SBR with fixed-time control. The presented method is valuable to offer a solution to realize and to maintain nitrogen removal via nitrite under normal conditions. | CUI You-wei PENG Yong-zhen GAN Xiang-qing YE Liu WANG Ya-yi | 2005 | Journal of Environmental Sciences2005，17，5： | 10 |
| 8 | Simulated Microgravity Conditions and Carbon Ion Irradiation Induce Spermatogenic Cell Apoptosis and Sperm DNA Damage显示文摘Objective To investigate the effect of simulated microgravity and carbon ion irradiation(CIR) on spermatogenic cell apoptosis and sperm DNA damage to the testis of male Swiss Webster mice,and assess the risk associated with space environment.Methods Sperm DNA damage indicated by DNA fragmentation index(DFI) and high DNA stainability(HDS) was measured by sperm chromatin structure assay(SCSA).Apoptosis of spermatogenic cells was detected by annexin V-propidium iodide assay.Bax(the expression levels of p53) and proliferating cell nuclear antigen(PCNA) were measured by immunoblotting;p53 and PCNA were located by immunohistology.Results HDS,DFI,apoptosis index,and the expression levels of p53 and Bax were detected to be significantly higher in the experimental groups(P<0.05) compared with those in the control group;however,the PCNA expression varied to a certain degree.p53-and PCNA-positive expression were detected in each group,mainly in relation to the spermatogonic cells and spermatocytes.Conclusion The findings of the present study demonstrated that simulated microgravity and CIR can induce spermatogenic cell apoptosis and sperm DNA damage.Sperm DNA damage may be one of the underlying mechanisms behind male fertility decline under space environment.These findings may provide a scientific basis for protecting astronauts and space traveler's health and safety. | LI Hong Yan ZHANG Hong MIAO Guo Ying XIE Yi SUN Chao DI Cui Xia LIU Yang LIU Yuan Yuan ZHANG Xin MA Xiao Fei XU Shuai GAN Lu ZHOU Xin | 2013 | Biomedical and Environmental Sciences2013，26，9： | 10 |
| 9 | Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq显示文摘抄写和 post-transcriptional 过程例如其他的拼接,在在 metazoans 控制发展节目起关键作用。最近出现的 RNA-seq 方法把我们真核细胞的 transcriptomes 的理解带到新水平,因为它能两个都同时解决基因表示水平和其他的拼接事件。在性腺获得细胞的区别的更好的理解,我们用 RNA-seq 从果蝇睾丸和卵巢分析了 mRNA 侧面。我们识别了有性特定的 isoforms 在的一套基因野类型(WT ) 性腺,包括几个抄写因素。我们发现从无差别的细菌房间的精子的区别导致了拼接的 RNA 的戏剧的 downregulation 因素。我们的数据证实 RNA 拼接事件是显著地在云石(欺骗) 的无差别的充实房间的袋子里更经常在在 WT 的区别之上的变异的睾丸,而是 downregulated 睾丸。与这一致,我们证明基因在 WT 为成熟分裂和终端区别要求睾丸主要在 transcriptional 水平被调整,然而并非由选择拼接。出人意料地,我们在在无差别的充实房间的欺骗睾丸改变因素和 histone 修改酶的染色质的所有家庭的表示观察了增加。更有趣地,有相反的酶的活动的染色质管理者和 histone 修改酶是在在睾丸的无差别的房间的 coenriched,建议这些房间可以拥有动态染色质建筑学。最后,我们的数据表明果蝇 gonadal transcriptomes 的许多新特征,和愿望导致的更全面的理解微分基因表示并且拼接在果蝇调整 gametogenesis。我们的数据为在果蝇在细菌房间生物学的许多有趣的区域拼接的基因表示和选择的系统的学习提供了一个基础,例如性同种二形和在 germline 干细胞系的增长对终端区别程序的规定的分子的基础。为未加工、分析的 RNA-seq 数据的 GEO 就职数字是 GSE16960。 | Qiang Gan Iouri Chepelev Gang Wei Lama Tarayrah Kairong Cui Keji Zhao Xin Chen | 2010 | Cell Research2010，20，7： | 9 |
| 10 | Involvement of the Mitochondrion-dependent and the Endoplasmic Reticulum Stress-signaling Pathways in Isoliquiritigenin-induced Apoptosis of HeLa Cell显示文摘Objective Isoliquiritigenin(ISL),a licorice chalconoid,is considered to be a bioactive agent with chemopreventive potential.This study investigates the mechanisms involved in ISL-induced apoptosis in human cervical carcinoma HeLa cells.Methods Cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT)assay.Apoptosis was determined by flow cytometry using an Annexin V-FITC Apoptosis Detection Kit.The intracellular ROS levels were assessed using a 2,7-dichlorofluorescein probe assay.The mitochondrial membrane potential was measured with the dual-emission potential-sensitive probe 5,5',6,6'-tetra-chloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide(JC-1).The degradation of poly-ADP-ribose polymerase(PARP)protein,the phosphorylation of PKR-like ER kinase(PERK),the phosphorylation of the α-subunit of eukaryotic initiation factor 2(eIF2α),the expression of the 78 kD glucose-regulated protein(GRP 78),and the activation of caspase-12 were analyzed via western blot analysis.Results ISL significantly inhibited the proliferation,the increase in ROS levels and apoptotic rates of HeLa cells in a concentration-dependent manner.Moreover,ISL induced mitochondrial dysfunction,caspase activation,and PARP cleavage,which displayed features of mitochondria dependent on apoptotic signals.Besides,exposure of HeLa cells to ISL triggered endoplasmic reticulum(ER)stress,as indicated by the increase in p-eIF2α and GRP78 expression,ER stress-dependent apoptosis is caused by the activation of ER-specific caspase-12.Conclusion The findings from our study suggest that ISL-induced oxidative stress causes HeLa cell apoptosis via the mitochondrion-dependent and the ER stress-triggered signaling pathways. | YUAN Xuan ZHANG Bo GAN Lu WANG Zhen Hua YU Ba Cui LIU Liang Liang ZHENG Qiu Sheng WANGZhi Ping | 2013 | Biomedical and Environmental Sciences2013，26，4： | 8 |
| 11 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 12 | Scale-up of a high shear wet granulation process using a nucleation regime map approach显示文摘 | Gan Luo Bing Xu Yi Zhang Xianglong Cui Jianyu Li Xinyuan Shi Yanjiang Qiao | 2017 | Particuology2017，15，2： | 7 |
| 13 | Epigenetic regulation of dental pulp stem cells and its potential in regenerative endodontics显示文摘Regenerative endodontics(RE)therapy means physiologically replacing damaged pulp tissue and regaining functional dentin–pulp complex.Current clinical RE procedures recruit endogenous stem cells from the apical papilla,periodontal tissue,bone marrow and peripheral blood,with or without application of scaffolds and growth factors in the root canal space,resulting in cementum-like and bone-like tissue formation.Without the involvement of dental pulp stem cells(DPSCs),it is unlikely that functional pulp regeneration can be achieved,even though acceptable repair can be acquired.DPSCs,due to their specific odontogenic potential,high proliferation,neurovascular property,and easy accessibility,are considered as the most eligible cell source for dentin–pulp regeneration.The regenerative potential of DPSCs has been demonstrated by recent clinical progress.DPSC transplantation following pulpectomy has successfully reconstructed neurovascularized pulp that simulates the physiological structure of natural pulp.The self-renewal,proliferation,and odontogenic differentiation of DPSCs are under the control of a cascade of transcription factors.Over recent decades,epigenetic modulations implicating histone modifications,DNA methylation,and noncoding(nc)RNAs have manifested as a new layer of gene regulation.These modulations exhibit a profound effect on the cellular activities of DPSCs.In this review,we offer an overview about epigenetic regulation of the fate of DPSCs;in particular,on the proliferation,odontogenic differentiation,angiogenesis,and neurogenesis.We emphasize recent discoveries of epigenetic molecules that can alter DPSC status and promote pulp regeneration through manipulation over epigenetic profiles. | Ying Liu Lu Gan Di-Xin Cui Si-Han Yu Yue Pan Li-Wei Zheng Mian Wan | 2021 | World Journal of Stem Cells2021，13，11： | 7 |
| 14 | Image Signature Based Mean Square Error for Image Quality Assessment显示文摘Motivated by the importance of Human visual system(HVS) in image processing, we propose a novel Image signature based mean square error(ISMSE)metric for full reference Image quality assessment(IQA).Efficient image signature based describer is used to predict visual saliency map of the reference image. The saliency map is incorporated into luminance difference between the reference and distorted images to obtain image quality score. The effect of luminance difference on visual quality with larger saliency value which is usually corresponding to foreground objects is highlighted. Experimental results on LIVE database release 2 show that by integrating the effects of image signature based saliency on luminance difference, the proposed ISMSE metric outperforms several state-of-the-art HVS-based IQA metrics but with lower complexity. | CUI Ziguan GAN Zongliang TANG Guijin LIU Feng ZHU Xiuchang | 2015 | Chinese Journal of Electronics2015，24，4： | 5 |
| 15 | Mesoporous Ag nanocubes synthesized via selectively oxidative etching at room temperature for surface- enhanced Raman spectroscopy显示文摘Silver nanocubes enriched with {100} facets have been extensively used for surface-enhanced Raman scattering. Herein, we report a new water-phase synthesis method for weU-defined Ag nanocubes with tunable sizes via a two-step procedure at room temperature. First, irregularly shaped Ag nanoparticles (INPs) were prepared by reducing silver ammonia solution using ethylal. Second, the agglomerated INPs were selectively etched with HNO3 and C1- to yield {100} facet-rich mesoporous Ag nanocubes. The mechanism of Ag-nanocube formation and growth was investigated in detail by elucidating the involved chemical reactions and physical changes at each step during the synthesis. The addition of C1- anions was responsible for facilitating Ag nanoparticle growth by removing surface-adsorbed Ag+ species, thereby eliminating inter-particle repulsive forces. This agglomeration was found crucial for the subsequent selective oxidation of Ag nanoparticles because the protective agent used, polyvinylpyrrolidone (PVP), was the most effective one for adsorption on the surfaces of Ag nanoparticles of size greater than approximately 50 nm. Importantly mesopores were found inside the Ag nanocubes; this can be attributed to the unavoidable imperfect packing during the agglomeration of INPs. The newly prepared Ag nanocubes were further used to enhance the Raman signal of rhodamine 6G, which is capable of reducing the detection limitation to 10-16 mol·L-1. | Lin Gan Meijia Yang Xi Ke Guofeng Cui Xudong Chen Shiva Gupta William Kellogg Drew Higgins Gang Wu | 2015 | Nano Research2015，8，7： | 4 |
| 16 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
| 17 | Spatial modulation of nanopattern dimensions by combining interference lithography and grayscale-patterned secondary exposure显示文摘Functional nanostructures are exploited for a variety of cutting-edge fields including plasmonics,metasurfaces,and biosensors,just to name a few.Some applications require nanostructures with uniform feature sizes while others rely on spatially varying morphologies.However,fine manipulation of the feature size over a large area remains a substantial challenge because mainstream approaches to precise nanopatterning are based on low-throughput pixel-by-pixel processing,such as those utilizing focused beams of photons,electrons,or ions.In this work,we provide a solution toward wafer-scale,arbitrary modulation of feature size distribution by introducing a lithographic portfolio combining interference lithography(IL)and grayscale-patterned secondary exposure(SE).Employed after the high-throughput IL,a SE with patterned intensity distribution spatially modulates the dimensions of photoresist nanostructures.Based on this approach,we successfully fabricated 4-inch wafer-scale nanogratings with uniform linewidths of<5%variation,using grayscale-patterned SE to compensate for the linewidth difference caused by the Gaussian distribution of the laser beams in the IL.Besides,we also demonstrated a wafer-scale structural color painting by spatially modulating the filling ratio to achieve gradient grayscale color using SE. | Zhuofei Gan Hongtao Feng Liyang Chen Siyi Min Chuwei Liang Menghong Xu Zijie Jiang Zhao Sun Chuying Sun Dehu Cui Wen-Di Li | 2022 | Light(Science & Applications)2022，11，5： | 3 |
| 18 | Coordinated transcription of ANRIL and P16 genes is silenced by P16 DNA methylation显示文摘Objective: To investigate the relationship between the transcription of ANRIL, P15, P14 and P16 at the same locus and the regulation mechanism of ANRIL.Methods: Publicly available database of Cancer Cell Line Encyclopedia(CCLE) was used in bioinformatic analyses. Methylation of Cp G islands was detected by denaturing high performance liquid chromatography(DHPLC). Gene transcript levels were determined using quantitative real-time polymerase chain reaction(q RTPCR) assays. An engineered P16-specific transcription factor and DNA methyltransferase were used to induce P16-specific DNA demethylation and methylation.Results: The expression level of ANRIL was positively and significantly correlated with that of P16 but not with that of P15 in the CCLE database. This was confirmed in human cell lines and patient colon tissue samples. In addition, ANRIL was significantly upregulated in colon cancer tissues. Transcription of ANRIL and P16 was observed only in cell lines in which the P16 alleles were unmethylated and not in cell lines with fully methylated P16 alleles.Notably, P16-specific methylation significantly decreased transcription of P16 and ANRIL in BGC823 and GES1 cells. In contrast, P16-specific demethylation re-activated transcription of ANRIL and P16 in H1299 cells(P<0.001).Alteration of ANRIL expression was not induced by P16 expression changes.Conclusions: ANRIL and P16 are coordinately transcribed in human cells and regulated by the methylation status of the P16 Cp G islands around the transcription start site. | Ying Gan Wanru Ma Xiuhong Wang Juanli Qiao Baozhen Zhang Chenghua Cui Zhaojun Liu Dajun Deng | 2018 | Chinese Journal of Cancer Research2018，30，1： | 2 |
| 19 | Chronic inflammation deteriorates structure and function of collagen fibril in rat temporomandibular joint disc显示文摘Collagen is the building component of temporomandibular joint(TMJ) discs and is often affected by inflammation in temporomandibular disorders. The macromechanical properties of collagen are deteriorated by chronic inflammation. However,the mechanism by which inflammation influences disc function remains unknown. The relationship between the ultrastructure and nanomechanical properties of collagen in inflamed discs should be clarified. Seven-week-old female Sprague–Dawley rats were randomly divided into two groups. Chronic TMJ inflammation was induced by intra-articular injection of complete Freund's adjuvant, and samples were harvested after 5 weeks. Picrosirius staining revealed multiple colours under polarized light, which represented alternative collagen bundles in inflamed discs. Using atomic force microscopy scanning, the magnitude of Young's modulus was reduced significantly accompanied with disordered collagen fibril arrangement with porous architecture of inflamed discs. Transmission electron microscopy scanning revealed a non-uniform distribution of collagen fibres, and oversized collagen fibrils were observed in inflamed discs. Fourier transform infrared microspectroscopy revealed a decrease in 1 338 cm^(-1)/amide II area ratio of collagen in different regions. The peak positions of amide I and amide II bands were altered in inflamed discs,indicating collagen unfolding. Our results suggest that sustained inflammation deteriorates collagen structures, resulting in the deterioration of the ultrastructure and nanomechanical properties of rat TMJ discs. | Shengjie Cui Yu Fu Yan Liu Xiaoxing Kou Jieni Zhang Yehua Gan Yanheng Zhou Xuedong Wang | 2019 | International Journal of Oral Science2019，11，1： | 2 |
| 20 | Protecting lithium metal anode in all-solid-state batteries with a composite electrolyte显示文摘The volume of the metallic lithium anode in allsolid-state Li metal batteries increases significantly due to the lithium dendrite formation during the battery cycling,and the rough surface of lithium metal also reduces Li-ion transport in Li/electrolyte interface.In this work,we developed a solid polymer composite by adding the lowcost Si_(3)N_(4)particles to protect the lithium anode in allsolid-state batteries.The Fourier transform infrared spectroscopy(FTIR)data show that the surface of 10 wt%Si_(3)N_(4)particles interacts with the polyethylene oxide(PEO)and lithium bis(trifluoromethanesulfonyl)imide(LiTFSI)salt;the interaction restricts the anion mobility and improves the ionic conductivity(1×10^(-4)S·cm^(-1))and lithium-ion transference number(0.28)of the composite electrolyte.The lithium metal anode is well protected by the composite electrolyte in all-solid-state cells,including symmetric and Li/LiFePO_(4)cells.The lithium dendrite growth suppression by this composite electrolyte indicates the possible application of these low-cost composite electrolytes for lithium metal protection. | Wen-Qing Wei Bing-Qiang Liu Yi-Qiang Gan Hai-Jian Ma Da-Wei Cui | 2021 | Rare Metals2021，40，2： | 2 |
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