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| 1 | Exogenous cytokinin application to Actinidia chinensis var. deliciosa ‘Hayward’ fruit promotes fruit expansion through water uptake显示文摘Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.deliciosa‘Hayward’fruit are very responsive to CPPU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is unclear.In this study,we hypothesised that CPPU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit DM.During fruit development,CPPU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate supply.We showed that CPPU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate limited.Enhanced glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CPPU-treated fruit and up-regulation of water channel aquaporin gene PIP2.4 at 49 days after anthesis.Transcipt analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate supply.At standard carbohydrate supply,the early glucose increase in CPPU fruit was associated with reduced starch synthesis and increased starch degradation.When carbohydrate supply was high,the early glucose increase in CPPU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase genes.We conclude that CPPU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited. | Simona Nardozza Helen L Boldingh Mark W Wohlers Andrew P Gleave Zhiwei Luo Guglielmo Costa Elspeth A MacRae Michael J Clearwater Annette C Richardson | 2017 | Horticulture Research2017，4，1： | 2 |
| 2 | Efficient transformation of Actinidia arguta by reducing the strength of basal salts in the medium to alleviate callus browning显示文摘 | Han M L Gleave A P Wang T C | 2010 | Plant B iotechnology Reporter2010，4，： | 1 |
| 3 | Transformation of citrus embryogenie cells using particle bombardment and production of transgenic embryos显示文摘 | Yao J L Wu J H Gleave A P | 1996 | Plant Sei1996，113，： | 1 |
| 4 | Analyses of expressed sequence tags from apple显示文摘 | NEWCOMB R D CROWHURST R N GLEAVE A P RIKKERINK E H A ALLAN A C BEUNING L L BOWEN J H GERA E JAMIESON K R JANSSEN B J LAING W A MCARTNEY S NAIN B ROSS G S SNOWDEN K C SOULEYRE E J F WALTON E F YAUK Y K | 2006 | Plant Physiology2006，141，： | 1 |
| 5 | Transformation of Citrus embryogenic cell using particle bombardment and production of transgenic embryos显示文摘 | Yao J L Wu J H Gleave A P | 1996 | Plant Sci1996，113，： | 1 |
| 6 | Transgenic overexpression of plasminogen activator inhibitor-1 promotes the development of polycystic ovarian changes in female mice显示文摘 | Devin J K Johnson J E Eren M Gleaves L A Bradham W S Bloodworth J R | 2007 | J Mol Endocrinol2007，39，： | 1 |
| 7 | Transformation of citrus embryogenic cells using particle bombardment and production of transgenic embryos显示文摘 | YAO J L WU J H GLEAVE A P MORRIS B A M | 1996 | Plant Science1996，113，： | 1 |
| 8 | Transformation of citrus rembryogenic cell using particle bombardment and production of transgenic embryos显示文摘 | Yao J L Wu J H Gleave A P | 1996 | Plant Sci1996，113，： | 1 |
| 9 | Macrophage lipoprotein lipase promotes foam cell formation and atherosclerosis in vivo 显示文摘 | Babaev V R Fazio S Gleaves L A | 1999 | J Clin Invest1999，103，12： | 1 |
| 10 | Transformation of Citrus embryogenic cell using particle bombardment and production of transgenic embryos显示文摘 | Yao J L Wu J H Gleave A P | 1996 | Plant Sci1996，113，： | 1 |
| 11 | Macrophage lipoprotein lipase promotes foam cell formation and atherosclerosis in vivo显示文摘 | BABAEV V R FAZIO S GLEAVES L A | 1999 | Journal of Clinical Investigation1999，103，12： | 1 |
| 12 | Analysis of expressed sequence tags from Actinidia:applications of across species EST database for gene discovery in the areas of flavor,health,color and ripening显示文摘 | CROWHURST R N GLEAVE A P MACRAE E A MPOMAH-DWAMENA C A ATKINSON R G BEUNING L L BULLEY S M CHAGNE D MARSH K B MATICH A J MONTEFIORI M NEWCOMB R D SCHAFFER R J USADEL B ALLAN A C BOLDINGH H L BOWEN J H DAVY M W ECKLOFF R FER-GUSON A R FRASER L G GERA E HELLENS R P JANSSENB J KLAGES K LO K R NAIN B MCNEILAGE M A DI-ARMID R M M RASSAM M RICHARDSON A C RIKKERINK EH A Ross G S SCHRODER R SNOWDEN K C SOULEYRE E JF TEMPLETON M D WALTON E F WANG D WANG M Y WANG Y Y WOOD M WU R M YAUK Y K LIANG W A | 2008 | BMC Genomics2008，9，351： | 1 |
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