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| 1 | Exogenous cytokinin application to Actinidia chinensis var. deliciosa ‘Hayward’ fruit promotes fruit expansion through water uptake显示文摘Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.deliciosa‘Hayward’fruit are very responsive to CPPU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is unclear.In this study,we hypothesised that CPPU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit DM.During fruit development,CPPU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate supply.We showed that CPPU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate limited.Enhanced glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CPPU-treated fruit and up-regulation of water channel aquaporin gene PIP2.4 at 49 days after anthesis.Transcipt analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate supply.At standard carbohydrate supply,the early glucose increase in CPPU fruit was associated with reduced starch synthesis and increased starch degradation.When carbohydrate supply was high,the early glucose increase in CPPU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase genes.We conclude that CPPU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited. | Simona Nardozza Helen L Boldingh Mark W Wohlers Andrew P Gleave Zhiwei Luo Guglielmo Costa Elspeth A MacRae Michael J Clearwater Annette C Richardson | 2017 | Horticulture Research2017，4，1： | 2 |
| 2 | Efficient transformation of Actinidia arguta by reducing the strength of basal salts in the medium to alleviate callus browning显示文摘 | Han M L Gleave A P Wang T C | 2010 | Plant B iotechnology Reporter2010，4，： | 1 |
| 3 | Transformation of citrus embryogenie cells using particle bombardment and production of transgenic embryos显示文摘 | Yao J L Wu J H Gleave A P | 1996 | Plant Sei1996，113，： | 1 |
| 4 | Clinical significance of the tumour capsule in the treatment of parotid pleomorphie adenomas 显示文摘 | McGurk M Renehan A Gleave EN | 1996 | Br J Surg1996，83，12： | 1 |
| 5 | Clinical significance of the tumour capsule in the treat- ment of parotid pleomorphic adenomas显示文摘 | MCGURK M RENEHAN A GLEAVE E N | 1996 | Br J Surg1996，83，： | 1 |
| 6 | Analyses of expressed sequence tags from apple显示文摘 | Newcomb R D Crowhurst R N Gleave A P | 2006 | Plant Physiol2006，141，： | 1 |
| 7 | Agrobacteriumand PEG-mediated transformation of the phytopathogenVenturia inaequalis显示文摘 | Fitzgerald A M Mudge A M Gleave A P | 2003 | MycologicalResearch2003，107，7： | 1 |
| 8 | Selectable marker-free transgenic plants without sexual cros-sing:transient expression of cre recombinase and use of a conditional lethal dominant gene显示文摘 | Gleave A P Mitra D S Mudge S R | 1999 | Plant Mole- cular Biology1999，40，2： | 1 |
| 9 | Progression to androgen independence is delayed by adjuvant treatment with antisense Bcl-2 oligodeoxynucleotides after castration in the LNCaP prostate tumor model显示文摘 | Gleave M Tolcher A Miyake H Nelson C Brown B Beraldi E | 1999 | Clin Cancer Res1999，5，： | 1 |
| 10 | Analysis of expressed sequence tags from apple显示文摘 | Newcomb R D Crowhurst R N Gleave A P | 2006 | Plant Physiol2006，141，： | 1 |
| 11 | Androgens and prostate cancer显示文摘 | So AI Hurtado-Coll A Gleave ME | 2003 | World J Urol2003，21，5： | 1 |
| 12 | A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA intothe plant genome显示文摘 | Gleave A P | | 0，，06： | 1 |
| 13 | Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis显示文摘 | BULLEY S M RASSAM M HOSER D OTTO W SCHUNEMANN N WRIGHT M MACRAE E GLEAVE A LAING W | 2009 | Journal of Experimental Botany2009，60，3： | 1 |
| 14 | Analyses of expressed sequence tags from apple显示文摘 | NEWCOMB R D CROWHURST R N GLEAVE A P RIKKERINK E H A ALLAN A C BEUNING L L BOWEN J H GERA E JAMIESON K R JANSSEN B J LAING W A MCARTNEY S NAIN B ROSS G S SNOWDEN K C SOULEYRE E J F WALTON E F YAUK Y K | 2006 | Plant Physiology2006，141，： | 1 |
| 15 | A versatile binary vector system with a T-DNA organizational structure conducive to efficient integration of cloned DNA into the plant genome显示文摘 | Gleave A P | 1992 | Plant Molecular Biology1992，20，6： | 1 |
| 16 | Progression to androgen independence is delayed by adjuvant treatment with anti-sene bcl - 2 oligodeoxynucleotides after castration in the LNCaP prostate tumor model显示文摘 | Gleave M Tolcher A Miyake H | 1999 | Clin Cancer Res1999，5，10： | 1 |
| 17 | Construct design for efficient,effective and highthroughput gene silencing in plant显示文摘 | Wesley S V Helliwell C A Smith N A Wang M B Rouse D T Liu Q Gooding P S Singh S P Abbott D Stoutjesdijk P A Robinson S P Gleave A P Green A G Waterhouse P M | 2001 | Plant Journal2001，27，6： | 1 |
| 18 | The multiple dimensions of perfec tionism and their relation with eating disorder features显示文摘 | Pearson C A Gleaves D H | 2006 | Per sonality and Individual Differences2006，41，2： | 1 |
| 19 | Mechanisms of the development of androgen independence in prostate caneer显示文摘 | So A Gleave M Hurtado-Col A | 2005 | World J Urol2005，23，1： | 1 |
| 20 | Salivary tumors--experience over thirty years显示文摘 | Gleave EN Whittaker JS Nicho|son A | 1979 | Clin Otolaryngol1979，4，4： | 1 |
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