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| 1 | LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration显示文摘The adult mammalian heart is thought to be a terminally differentiated organ given the postmitotic nature of cardiomyocytes.Consequently,the potential for cardiac repair through cardiomyocyte proliferation is extremely limited.Low-density lipoprotein receptor-related protein 6(LRP6)is a Wnt co-receptor that is required for embryonic heart development.In this study we investigated the role of LRP6 in heart repair through regulation of cardiomyocyte proliferation.Lrp6 deficiency increased cardiomyocyte cell cycle activity in neonatal,juvenile and adult mice.Cardiomyocyte-specific deletion of Lrp6 in the mouse heart induced a robust regenerative response after myocardial infarction(MI),led to reduced MI area and improvement in left ventricular systolic function.In vivo genetic lineage tracing revealed that the newly formed cardiomyocytes in Lrp6-deficient mouse hearts after MI were mainly derived from resident cardiomyocytes.Furthermore,we found that the pro-proliferative effect of Lrp6 deficiency was mediated by the ING5/P21 signaling pathway.Gene therapy using the adeno-associated virus(AAV)9 miRNAi-Lrp6 construct promoted the repair of heart injury in mice.Lrp6 deficiency also induced the proliferation of human induced pluripotent stem cell-derived cardiomyocytes(iPSC-CMs).Our study identifies LRP6 as a critical regulator of cardiomyocyte proliferation,which may lead to the development of a novel molecular strategy to promote myocardial regeneration and repair. | Yahan Wu Liping Zhou Hongyu Liu Ran Duan Huixing Zhou Fulei Zhang Xiaoyu He Dongbo Lu Ke Xiong Maolin Xiong Jinzhu Zhuang Yi Liu Li Li Dandan Liang Yi-Han Chen | 2021 | Cell Research2021，31，4： | 5 |
| 2 | Nonlinear dynamic responses of sandwich functionally graded porous cylindrical shells embedded in elastic media under 1:1 internal resonance显示文摘In this article, the nonlinear dynamic responses of sandwich functionally graded(FG) porous cylindrical shell embedded in elastic media are investigated. The shell studied here consists of three layers, of which the outer and inner skins are made of solid metal, while the core is FG porous metal foam. Partial differential equations are derived by utilizing the improved Donnell's nonlinear shell theory and Hamilton's principle. Afterwards, the Galerkin method is used to transform the governing equations into nonlinear ordinary differential equations, and an approximate analytical solution is obtained by using the multiple scales method. The effects of various system parameters,specifically, the radial load, core thickness, foam type, foam coefficient, structure damping,and Winkler-Pasternak foundation parameters on nonlinear internal resonance of the sandwich FG porous thin shells are evaluated. | Yunfei LIU Zhaoye QINT Fulei CHU | 2021 | Applied Mathematics and Mechanics(English Edition)2021，42，6： | 4 |
| 3 | Redox-sensitive micelles for targeted intracellular delivery and combination chemotherapy of paclitaxel and all-trans-retinoid acid显示文摘The application of paclitaxel(PTX) in clinic has been restricted due to its poor solubility.Several traditional nano-medicines have been developed to improve this defect,while they are still lack of tumor targeting ability and rapid drug release. In this work,an amphiphilic polymeric micelle of hyaluronic acid(HA) – all-trans-retinoid acid(ATRA) with a disulfide bond,was developed successfully for the co-delivery of PTX and ATRA. The combination chemotherapy of PTX and ATRA can strengthen the anti-tumor activity. Along with selfassembling to micelles in water,the delivery system displayed satisfying drug loading capacities for both PTX(32.62% ± 1.39%) and ATRA,due to directly using ATRA as the hydrophobic group. Rapid drug release properties of the PTX-loaded redox-sensitive micelles(HA-SS-ATRA) in vitro were confirmed under reducing condition containing GSH. Besides,HA-CD44 mediated endocytosis promoted the uptake of HA-SS-ATRA micelles by B16 F10 cells. Due to these properties,cytotoxicity assay verified that PTX-loaded HA-SS-ATRA micelles showed concentration-dependent cytotoxicity and displayed obvious combination therapy of PTX and ATRA. Importantly,HA-SS-ATRA micelles could remarkably prolong plasma circulation time after intravenously administration. Therefore,redox-sensitive HASS-ATRA micelles could be utilized and explored as a promising drug delivery system for cancer combination chemotherapy. | Lingfei Han Lejian Hu Fulei Liu XinWang Xiaoxian Huang Bowen Liu Feng Feng Wenyuan Liu Wei Qu | 2019 | Asian Journal of Pharmaceutical Sciences2019，14，5： | 2 |
| 4 | Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons显示文摘Activation of the heart normally begins in the sinoatrial node(SAN).Electrical impulses spontaneously released by SAN pacemaker cells(SANPCs)trigger the contraction of the heart.However,the cellular nature of SANPCs remains controversial.Here,we report that SANPCs exhibit glutamatergic neuron-like properties.By comparing the single-cell transcriptome of SANPCs with that of cells from primary visual cortex in mouse,we found that SANPCs co-clustered with cortical neurons.Tissue and cellular imaging confirmed that SANPCs contained key elements of glutamatergic neurotransmitter system,expressing genes encoding glutamate synthesis pathway(G/s),ionotropic and metabotropic glutamate receptors(Grina,Gria3,Grm1 and Grm5)t and glutamate transporters(Slc17a7).SANPCs highly expressed cell markers of glutamatergic neurons(Snap25 and S/-c17a7)t whereas Gad1,a marker of GABAergic neurons,was negative.Functional studies revealed that inhibition of glutamate receptors or transporters reduced spontaneous pacing frequency of isolated SAN tissues and spontaneous Ca2+transients frequency in single SANPC.Collectively,our work suggests that SANPCs share dominant biological properties with glutamatergic neurons,and the glutamatergic neurotransmitter system may act as an intrinsic regulation module of heart rhythm,which provides a potential intervention target for pacemaker cell-associated arrhythmias. | Dandan Liang Zhigang Xue Jinfeng Xue Duanyang Xie Ke Xiong Huixing Zhou Fulei Zhang Xuling Su Guanghua Wang Qicheng Zou Yi Liu Jian Yang Honghui Ma Luying Peng Chunyu Zeng Gang Li Li Wang Yi-Han Chen | 2021 | Protein & Cell2021，12，7： | 1 |
| 5 | Nonlinear characteristic investigation of magnetorheological damper-rotor system with local nonlinearity显示文摘Magnetorheological(MR)dampers show superior performance in reducing rotor vibration,but their high nonlinearity will cause nonsynchronous response,resulting in fatigue and instability of rotors.Herein,we are devoted to the investigation of the nonlinear characteristics of MR damper mounted on a flexible rotor.First,Reynolds equations with bilinear constitutive equations of MR fluid are employed to derive nonlinear oil film forces.Then,the Finite Element(FE)model of rotor system is developed,where the local nonlinear support forces produced by MR damper and its coupling effects with the rotor are considered.A hybrid numerical method is proposed to solve the nonlinear FE motion equations of the MR damper-rotor system.To validate the proposed model,a rotor test bench with two dual-coil MR dampers is constructed,upon which experimental studies on the dynamic characteristics of MR damper-rotor system are carried out.The effects of different system parameters,including rotational speed,excitation current and amount of unbalance,on nonlinear dynamic behaviors of MR damper-rotor system are evaluated.The results show that the system may appear chaos,jumping,and other complex nonlinear phenomena,and the level of the nonlinearity can be effectively alleviated by applying suitable excitation current and oil supply pressure. | Jun WANG Yunfei LIU Zhaoye QIN Liang MA Fulei CHU | 2023 | Chinese Journal of Aeronautics2023，36，2： | 1 |
| 6 | Dynamic characteristics of flow induced vibration in a rotor-seal system显示文摘 | Nan Zhang Ying Liu Fulei Chu | 2011 | Shock and Vibration2011，18，： | 1 |
| 7 | Short-time Fourier Transform Using Odd Symmetric Window Function显示文摘In this paper,a novel time–frequency(TF)analysis method,called the short-time Fourier transform using odd symmetric window function(OSTFT),is proposed using odd symmetric window function to replace the conventional even window function of STFT.Different from conventional STFT acquiring the amplitude maximum at time and frequency centers,OSTFT acquires the minimum amplitude of 0.Hence,OSTFT can obtain a TF representation(TFR)with high TF resolution by utilizing the leaked energy rather than restraining it.It is worth to mention that the proposed OSTFT can vitiate the effect of window size we choose on the TFR obtained.Furthermore,it also has a good performance on signals with complex instantaneous frequencies(IFs),even crossing IFs.Because we just replace the conventional window function of STFT,the time-consuming of the proposed OSTFT is at the same level as the conventional STFT.The effectiveness of proposed OSTFT has been validated on two complex multi-component simulated numerical signals and a signal collected from the brown bat. | Miaofen Li Youmin Liu Shaodan Zhi Tianyang Wang Fulei Chu | 2022 | Journal of Dynamics, Monitoring and Diagnostics2022，1，1： | 0 |
| 8 | Understanding the alkyl effect of geminal dinitropropyl ester energetic plasticizers on hydroxyl terminated polybutadiene(HTPB):Simultaneous tuning on low temperature behavior and processability显示文摘Geminal dinitropropyl ester plasticizers(DNPEPs) possess excellent energetic performances which provide good potentials as insensitive plasticizer. In this study, we design and synthesize DNPEPs with different alkane chain parts, and systematically investigate their structure-property relationships.Results show that DNPEPs have impact sensitivities all higher than 25.2 J, thermal decomposition temperatures all higher than 254 ℃, and glass transition temperatures(T_(g)) lower than-90 ℃.Furthermore, the effects of DNPEPs as plasticizer are studied on hydroxyl terminated polybutadiene(HTPB) in detail, including the viscosity, glass transition temperatures and others. It is noteworthy that 2,2-dinitropropyl nonanoate(DNPNc) among these DNPEPs exhibits the most expected simultaneous tuning effects on both viscosity and T_(g) of HTPB systems, providing favorable potentials to replace the conventional plastizers as dioctyl sebacate(DOS) in the HTPB based propellants and explosives. | Baodong Zhao Yinglei Wang Fulei Gao Yajing Liu Weixiao Liu Feng Ding | 2023 | Chinese Journal of Chemical Engineering2023，54，2： | 0 |
| 9 | Multiple internal resonances of rotating composite cylindrical shells under varying temperature fields显示文摘Composite cylindrical shells,as key components,are widely employed in large rotating machines.However,due to the frequency bifurcations and dense frequency spectra caused by rotation,the nonlinear vibration usually has the behavior of complex multiple internal resonances.In addition,the varying temperature fields make the responses of the system further difficult to obtain.Therefore,the multiple internal resonances of composite cylindrical shells with porosities induced by rotation with varying temperature fields are studied in this paper.Three different types of the temperature fields,the Coriolis forces,and the centrifugal force are considered here.The Hamilton principle and the modified Donnell nonlinear shell theory are used to obtain the equilibrium equations of the system,which are transformed into the ordinary differential equations(ODEs)by the multi-mode Galerkin technique.Thereafter,the pseudo-arclength continuation method,which can identify the regions of instability,is introduced to obtain the numerical results.The detailed parametric analysis of the rotating composite shells is performed.Multiple internal resonances caused by the interaction between backward and forward wave modes and the energy transfer phenomenon are detected.Besides,the nonlinear amplitude-frequency response curves are different under different temperature fields. | Yunfei LIU Jun WANG Jiaxin HU Zhaoye QIN Fulei CHU | 2022 | Applied Mathematics and Mechanics(English Edition)2022，43，10： | 0 |
| 10 | Vibration Characteristics of Rotor System with Loose Disc Caused by the Insufficient Interference Force显示文摘The rotating parts looseness is one of the common failures in rotating machinery.The current researches of looseness fault mainly focus on non-rotating components.However,the looseness fault of disc-shaft system,which is the main work part in the rotor system,is almost ignored.Here,a dynamic model of the rotor system with loose disc caused by the insufficient interference force is proposed based on the contact model of disc-shaft system with the microscopic surface topography,the vibration characteristics of the system are analyzed and discussed by the number simulation,and verified by the experiment.The results show that the speed of the shaft,the contact stiffness,the clearance between the disc and shaft,the damping of the disc and the rotational damping have an influence on the rotation state of the disc.When the rotation speed of the disc and the shaft are same,the collision frequency is mainly composed of one frequency multiplication component and very weak high frequency multiplication components.When the rotation speed of the disc and the shaft is close,the vibration of the disc occurs a beat vibration phenomenon in the horizontal direction.Simultaneously,a periodical similar beat vibration phenomenon also occurs in the waveform of the disc-shaft displacement difference.The collision frequency is mainly composed of a low frequency and a weak high frequency component.When the rotation speed of the disc and the shaft has great difference,the collision frequency is mainly composed of one frequency multiplication,a few weak high frequency multiplication components and a few low frequency multiplication component.With the reduction of the relative speed of the disc,the trajectory of the disc changes from circle-shape to inner eight-shape,and then to circle-shape.In the inner eight-shape,the inner ring first gradually becomes smaller and then gradually becomes larger,and the outer ring is still getting smaller.The obtained research results in this paper has important theoretical value for the diagnosis of the rotor system with the loose disc. | Zhinong Li Fang Qiao Wenxiu Lu Jie Liu Dong Wang Fulei Chu | 2022 | Chinese Journal of Mechanical Engineering2022，35，3： | 0 |
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