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| 1 | Hypoxia induces H2O2production and activates antioxidant defence system in grapevine buds through mediation of H2O2and ethylene显示文摘 | Ricardo V Francisca P Sebastián R | 2012 | Experimental Botany2012，63，11： | 1 |
| 2 | Harvest prediction in Algerie' loquat 显示文摘 | Hueso J Mercedes P Z Francisca A | 2007 | International Journal of Biomete- orology2007，51，： | 1 |
| 3 | Structure of calcium silicate hydrates formed in alkaline-activated slag: influence of the type of alkalineactivator显示文摘 | Ana F J Francisca P Isabel S | | Journal of the American Ceramic Society0，，： | 1 |
| 4 | Biodegradation of crude oil in sandy sediment显示文摘 | Jussara P Del'Arco Francisca P | 1999 | International Biodeterioration & Biodegradation1999，44，： | 1 |
| 5 | Fluorescent probes for the detection of reactive oxygen species in human spermatozoa显示文摘Reactive oxygen species(ROS)production is a by-product of mitochondrial activity and is necessary for the acquisition of the capacitated state,a requirement for functional spermatozoa.However,an increase in oxidative stress,due to an abnormal production of ROS,has been shown to be related to loss of sperm function,highlighting the importance of an accurate detection of sperm ROS,given the specific nature of this cell.In this work,we tested a variety of commercially available fluorescent probes to detect ROS and reactive nitrogen species(RNS)in human sperm,to define their specificity.Using both flow cytometry(FC)and fluorescence microscopy(FM),we confirmed that MitoSOX™Red and dihydroethidium(DHE)detect superoxide anion(as determined using antimycin A as a positive control),while DAF-2A detects reactive nitrogen species(namely,nitric oxide).For the first time,we also report that RedoxSensor™Red CC-1,CellROX®Orange Reagent,and MitoPYl seem to be mostly sensitive to hydrogen peroxide,but not superoxide.Furthermore,mean fluorescence intensity(and not percentage of labeled cells)is the main parameter that can be reproducibly monitored using this type of methodology. | Sara Escada-Rebelo Francisca G Mora Ana P Sousa Teresa Almeida-Santos Artur Paiva Joao Ramalho-Santos | 2020 | Asian Journal of Andrology2020，22，5： | 1 |
| 6 | Engineering baker' S yeast : room for improvement 显示文摘 | Francisca R Pascual S Jose A P | 1999 | TiBTECH1999，17，： | 1 |
| 7 | A Novel surgical ap- proach to sow-trnnsit constipation:Report of two cases 显示文摘 | GEORGE P FRANCISCA L MARIA EM | 2008 | Dis Colon Rectum2008，51，： | 1 |
| 8 | A Novel Surgicalapproach to slow-transit constipation:report of two cases显示文摘 | George P Francisca L María EM | | 0，，01： | 1 |
| 9 | A Randomised Study of Midazolam for Sedation in Flexible Bronchoscopy显示文摘 | Enrique Cases Viedma Javier Pérez Pallarés Miguel ángel Martínez García Raquel López Reyes Francisca Sanchís Moret José Luis Sanchís Aldás | 2010 | Archivos de Bronconeumología (Internet)2010，，6： | 1 |
| 10 | Isolation, purification and characterization of a Cold-Active lipase from Aspergillus nidulans 显示文摘 | Isabel M Francisca R G Jose A P | 2000 | J Agric food Chem2000，48，： | 1 |
| 11 | Amantadine reduces the duration of levodopa-induced dyskinesia: A randomized, double-blind, placebo-controlled study 显示文摘 | FRANCISCO P PEDRO B FRANCISCA S | 2005 | Paikinsouism Related Disorders2005，11，7： | 1 |
| 12 | Effect of curing temperature on cement hydration 显示文摘 | Imad E Marta P Francisca P | 2009 | Ceramics-Silikaty2009，53，2： | 1 |
| 13 | Influence of the chemical structure of urethane-based thickeners on the properties of waterborne polyurethane adhesives显示文摘 | ELENA O C FRANCISCA A A ANA T P | 2009 | The Journal of Adhesion2009，85，10： | 1 |
| 14 | Characterization of waterborne polyurethane adhesives containing different amounts of ionic groups显示文摘 | M. Angeles Pérez-Limi?ana Francisca Arán-Aís Ana M. Torró-Palau A. César Orgilés-Barceló José Miguel Martín-Martínez | 2005 | International Journal of Adhesion and Adhesives2005，，6： | 1 |
| 15 | Ex pression of adipose microRNAs is sensitive to dietaryconjugated linoleic acid treatment in mice显示文摘 | PILAR P FRANCISCA S ANDREU P | 2008 | PLoS One2008，5，13： | 1 |
| 16 | Evolution of lipopolysaccharide (LPS) recognition and signaling:Fish TLR4 does not recognize LPS and negatively regulates NF-{kappa}B activation显示文摘 | Maria P S Francisca A P Azucena L M | 2009 | J Immunol2009，182，： | 1 |
| 17 | Exploring seven hundred years of transhumance, climate dynamic, fire and human activity through a historical mountain pass in central Spain显示文摘A high-altitude peat sequence from the heart of the Spanish Central System(Gredos range) was analysed through a multi-proxy approach to determine the sensitivity of high-mountain habitats to climate, fire and land use changes during the last seven hundred years, providing valuable insight into our understanding of the vegetation history and environmental changes in a mountain pass close to a traditional route of transhumance. The pollen data indicate that the vegetation was dominated by shrublands and grasslands with scattered pines in high-mountain areas, while in the valleys cereals, chestnut and olive trees were cultivated. Strong declines of high-mountain pines percentages are recorded at 1540, 1675, 1765, 1835 and 1925 cal AD, which may be related to increasing grazing activities and/or the occurrence of anthropogenic fires. The practice of mountain summer farming and transhumance deeply changed and redesigned the landscape of the high altitudes in central Spain(Gredos range) since the Middle Ages, although its dynamics was influenced in some way by climate variability of the past seven centuries. | José Antonio LóPEZ-SáEZ Francisca ALBA-SáNCHEZ Sandra ROBLES-LóPEZ Sebastián PéREZ-DíAZ Daniel ABEL-SCHAAD Silvia SABARIEGO-RUIZ Arthur GLAIS | 2016 | Journal of Mountain Science2016，13，7： | 0 |
| 18 | Molecular analysis of pancreatic cystic neoplasm in routine clinical practice显示文摘BACKGROUND Cystic pancreatic lesions consist of a wide variety of lesions that are becoming increasingly diagnosed with the growing use of imaging techniques.Of these,mucinous cysts are especially relevant due to their risk of malignancy.However,morphological findings are often suboptimal for their differentiation.Endoscopic ultrasound fine-needle aspiration(EUS-FNA)with molecular analysis has been suggested to improve the diagnosis of pancreatic cysts.AIM To determine the impact of molecular analysis on the detection of mucinous cysts and malignancy.METHODS An 18-month prospective observational study of consecutive patients with pancreatic cystic lesions and an indication for EUS-FNA following European clinical practice guidelines was conducted.These cysts included those>15 mm with unclear diagnosis,and a change in follow-up or with concerning features in which results might change clinical management.EUS-FNA with cytological,biochemical and glucose and molecular analyses with next-generation sequencing were performed in 36 pancreatic cysts.The cysts were classified as mucinous and non-mucinous by the combination of morphological,cytological and biochemical analyses when surgery was not performed.Malignancy was defined as cytology positive for malignancy,high-grade dysplasia or invasive carcinoma on surgical specimen,clinical or morphological progression,metastasis or death related to neoplastic complications during the 6-mo follow-up period.Next-generation sequencing results were compared for cyst type and malignancy.RESULTS Of the 36 lesions included,28(82.4%)were classified as mucinous and 6(17.6%)as non-mucinous.Furthermore,5(13.9%)lesions were classified as malignant.The amount of deoxyribonucleic acid obtained was sufficient for molecular analysis in 25(69.4%)pancreatic cysts.The amount of intracystic deoxyribonucleic acid was not statistically related to the cyst fluid volume obtained from the lesions.Analysis of KRAS and/or GNAS showed 83.33%[95%confidence interval(CI):63.34-100]sensitivity,60%(95%CI:7.06-100)specificity,88.24%(95%CI:69.98-100)positive predictive value and 50%(95%CI:1.66-98.34)negative predictive value(P=0.086)for the diagnosis of mucinous cystic lesions.Mutations in KRAS and GNAS were found in 2/5(40%)of the lesions classified as non-mucinous,thus recategorizing those lesions as mucinous neoplasms,which would have led to a modification of the follow-up plan in 8%of the cysts in which molecular analysis was successfully performed.All 4(100%)malignant cysts in which molecular analysis could be performed had mutations in KRAS and/or GNAS,although they were not related to malignancy(P>0.05).None of the other mutations analyzed could detect mucinous or malignant cysts with statistical significance(P>0.05).CONCLUSION Molecular analysis can improve the classification of pancreatic cysts as mucinous or non-mucinous.Mutations were not able to detect malignant lesions. | Raquel Herranz Pérez Felipe de la Morena López Pedro L Majano Rodríguez Francisca Molina Jiménez Lorena Vega Piris Cecilio Santander Vaquero | 2021 | World Journal of Gastrointestinal Endoscopy2021，13，2： | 0 |
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