|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Matrix metalloproteinase-9 contributes to parenchymal hemorrhage and necrosis in the remnant liver after extended hepatectomy in mice显示文摘AIM:To investigate the effect of matrix metalloproteinase-9(MMP-9) on the remnant liver after massive hepatectomy in the mouse.METHODS:Age-matched,C57BL/6 wild-type(WT),MMP-9(-/-),and tissue inhibitors of metalloproteinases(TIMP)-1(-/-) mice were used.The mice received 80%-partial hepatectomy(PH).Samples were obtained at 6 h after 80%-PH,and we used histology,immunohistochemical staining,western blotting analysis and zymography to investigate the effect of PH on MMP-9.The role of MMP-9 after PH was investigated using a monoclonal antibody and MMP inhibitor.RESULTS:We examined the remnant liver 6 h after 80%-PH and found that MMP-9 deficiency attenuated the formation of hemorrhage and necrosis.There were significantly fewer and smaller hemorrhagic and necrotic lesions in MMP-9(-/-) remnant livers compared with WT and TIMP-1(-/-) livers(P < 0.01),with no difference between WT and TIMP-1(-/-) mice.Serum alanine aminotransaminase levels were significantly lower in MMP-9(-/-) mice compared with those in TIMP-1(-/-) mice(WT:476 ± 83 IU/L,MMP-9(-/-):392 ± 30 IU/L,TIMP-1(-/-):673 ± 73 IU/L,P < 0.01).Western blotting and gelatin zymography demonstrated a lack of MMP-9 expression and activity in MMP-9(-/-) mice,which was in contrast to WT and TIMP-1(-/-) mice.No change in MMP-2 expression was observed in any of the study groups.Similar to MMP-9(-/-) mice,when WT mice were treated with MMP-9 monoclonal antibody or the synthetic inhibitor GM6001,hemorrhagic and necrotic lesions were significantly smaller and fewer than in control mice(P < 0.05).These results suggest that MMP-9 plays an important role in the development of parenchymal hemorrhage and necrosis in the small remnant liver.CONCLUSION:Successful MMP-9 inhibition attenuates the formation of hemorrhage and necrosis and might be a potential therapy to ameliorate liver injury after massive hepatectomy. | Norifumi Ohashi Tomohide Hori Florence Chen Sura Jermanus Christopher B Eckman Akimasa Nakao Shinji Uemoto Justin H Nguyen | 2012 | World Journal of Gastroenterology2012，18，19： | 3 |
| 2 | Design and Hydraulic Characteristics of A Field-Scale Bi-phasic Bioretention Rain Garden System for Storm Water Management显示文摘 | Yang H Florence DC McCoy EL Dick WA Grewal PS | | 0，，： | 1 |
| 3 | Spend residue from cumin-A potential source of dietary fiber显示文摘 | SOWBHAGYA H B FLORENCE P S | 2007 | Food Chemistry2007，104，： | 1 |
| 4 | 查看详情显示文摘 | G M Espallargas L Brammer J van de Streek K Shankland A J Florence H Adams | | 0，，： | 1 |
| 5 | The placenta: a main role in congenital toxoplasmosis?显示文摘 | Florence Robert-Gangneux Jean-Benjamin Murat Hélène Fricker-Hidalgo Marie-Pierre Brenier-Pinchart Jean-Pierre Gangneux Hervé Pelloux | 2011 | Trends in Parasitology2011，，12： | 1 |
| 6 | Dynamic plastic flow buckling of short cylindrical shells due to impulsive loading显示文摘 | Florence A Vaughan H | 1968 | Journal Solids and Structures1968，4，： | 1 |
| 7 | Spent residace from cumin--a potential source of dietary fiber显示文摘 | SOWBHAGYA H B FLORENCE S P MAHADEVAMMA S | 2007 | Food Chemistry2007，，4： | 1 |
| 8 | Matrix metalloproteinase-9 in the initial injury after hepatectomy in mice显示文摘AIM:To investigate the role of matrix metalloproteinase(MMP)-9 in the pathogenesis of postoperative liver failure(PLF) after extended hepatectomy(EH).METHODS:An insufficient volume of the remnant liver(RL) results in higher morbidity and mortality,and a murine model with 80%-hepatectomy was used.All investigations were performed 6 h after EH.Mice were first divided into two groups based on the postoperative course(i.e.,the PLF caused or did not),and MMP-9 expression was measured by Western blotting.The source of MMP-9 was then determined by immunohistological stainings.Tissue inhibitor of metalloproteinase(TIMP)-1 is the endogenous inhibitor of MMP-9,and MMP-9 behavior was assessed by the experiments in wild-type,MMP-9(-/-) and TIMP-1(-/-) mice by Western blotting and gelatin zymography.The behavior of neutrophils was also assessed by immunohistological stainings.An anti-MMP-9 monoclonal antibody and a broadspectrum MMP inhibitor were used to examine the role of MMP-9.RESULTS:Symptomatic mice showed more severe PLF(histopathological assessments:2.97 ± 0.92 vs 0.11 ± 0.08,P < 0.05) and a higher expression of MMP-9(71085 ± 18274 vs 192856 ± 22263,P < 0.01).Nonnative leukocytes appeared to be the main source of MMP-9,because MMP-9 expression corresponding with CD11b positive-cell was observed in the findings of immunohistological stainings.In the histopathological findings,the PLF was improved in MMP-9(-/-) mice(1.65% ± 0.23% vs 0.65% ± 0.19%,P < 0.01) and it was worse in TIMP-1(-/-) mice(1.65% ± 0.23% vs 1.78% ± 0.31%,P < 0.01).Moreover,neutrophil migration was disturbed in MMP-9(-/-) mice in the immunohistological stainings.Two methods of MMP-9 inhibition revealed reduced PLF,and neutrophil migration was strongly disturbed in MMP-9-blocked mice in the histopathological assessments(9.6 ± 1.9 vs 4.2 ± 1.2,P < 0.05,and 9.9 ± 1.5 vs 5.7 ± 1.1,P < 0.05).CONCLUSION:MMP-9 is important for the process of PLF.The initial injury is associated with MMP-9 derived from neutrophils,and MMP-9 blockade reduces PLF.MMP-9 may be a potential target to prevent PLF after EH and to overcome an insufficient RL. | Norifumi Ohashi Tomohide Hori Florence Chen Sura Jermanus Akimasa Nakao Shinji Uemoto Justin H Nguyen | 2013 | World Journal of Gastroenterology2013，19，20： | 1 |
| 9 | Plasma carotenoids and vitamin C concentrations and risk of urothelial cell carcinoma in the European Prospective Investigation into Cancer and Nutrition12345显示文摘 | Martine M Ros H Bas Bueno-de-Mesquita Ellen Kampman Katja KH Aben Frederike L Büchner Eugene HJM Jansen Carla H van Gils Lars Egevad Kim Overvad Anne Tj?nneland Nina Roswall Marie Christine Boutron-Ruault Marina Kvaskoff Florence Perquier Rudolf Kaaks Jen | 2012 | American Journal of Clinical Nutrition2012，，4： | 1 |
| 10 | Effect of myocardial viability assessed by 99Tcm-sestamibi SPECT and 18F-FDG PET on clinical outcome in coronary artery disease显示文摘 | Carsten A Florence H | 1997 | J Nucl Med1997，38，： | 1 |
| 11 | Evaluation of enzyme-assisted extraction on quality of garlic volatile oil显示文摘 | Sowbhagya H Purnima K Florence S | 2009 | Food Chemistry2009，113，： | 1 |
| 12 | Oblongifolins benzoylphlucinol A - D, polyprenylated derivatives from Garcinia oblongifolia 显示文摘 | Waffa H Solenn B Florence MB | 2006 | J Nat Prod2006，69，8： | 1 |
| 13 | Structural changes of rareearth-doped, nanostructured zirconia solid solution 显示文摘 | Florence Boulc' h Elisabeth Djurado | 2003 | Solid State Ionics2003，157，： | 1 |
| 14 | Is the yield stress of aqueous foam a well-defined quantity显示文摘 | Florence Rouyer Sylvie Cohen-Addad Reinhard H¨ohler | | 0，，1： | 1 |
| 15 | Research criteria for the diagnosis of Alzheimer’s disease: revising the NINCDS–ADRDA criteria显示文摘 | Bruno Dubois Howard H Feldman Claudia Jacova Steven T DeKosky Pascale Barberger-Gateau Jeffrey Cummings André Delacourte Douglas Galasko Serge Gauthier Gregory Jicha Kenichi Meguro John O’Brien Florence Pasquier Philippe Robert Martin Rossor Steven Sallow | 2007 | Lancet Neurology2007，，： | 1 |
| 16 | Applications of Lanthanide Trichloride Hydrates Prepared from Mischmetall in Luche-Type Reduction显示文摘 | Marie-Isabelle Lannou Florence Hélion Jean-Louis Namy | 2007 | Synlett2007，，17： | 1 |
| 17 | Titanate-based ceramics for separated long-lived radionuclides显示文摘 | Catherine Fillet Thierry Advocat Florence Bart Gilles Leturcq Hélène Rabiller | 2004 | Comptes rendus - Chimie2004，，： | 1 |
| 18 | Evaluation of enzyme-assisted extraction on quality of garlic volatile oil显示文摘 | Sowbhagya H B Purnima K T Florence S P | 2009 | Food Chem2009，113，4： | 1 |
| 19 | Loss of chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landraces to high-light and heat stress显示文摘 | Michel H Florence T | 1999 | Australian Journal of Plant Physiolo- gy1999，26，： | 1 |
| 20 | Assessment and treat- ment of stereotypic behavior in children with autism and other devel- opmental disabilities: a thirty year review显示文摘 | FLORENCE D D JASON M H SARAH R H | 2012 | Res Autism Spect Dis- orders2012，6，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员