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| 1 | N-acetylcysteine modulates angiogenesis and vasodilation in stomach such as DNA damage in blood of portal hypertensive rats显示文摘AIM: To evaluate the antioxidant effect of N-acetylcysteine(NAC) on the stomach of rats with portal hypertension.METHODS: Twenty-four male Wistar rats weighing ± 250 g were divided into four experimental groups(n =6 each): Sham-operated(SO),SO + NAC,partial portal vein ligation(PPVL),and PPVL + NAC. Treatment with NAC in a dose of 10 mg/kg(i.p.) diluted in 0.6 m L of saline solution was administered daily for 7 d starting 8 d after the surgery. Animals from the PPVL and SO group received saline solution(0.6 m L) for the same period of time as the PPVL + NAC and SO + NAC group. On the 15 th day the animals were anesthetized and we evaluated portal pressure by cannulating mesenteric artery. After,we removed the stomach for further analysis. We performed immunohistochemical analysis for endothelial nitric oxide synthase(e NOS),vascular endothelial growth factor(VEGF),and nitrotirosine(NTT) proteins in stomach. We also evaluated e NOS and VEGF by Western blot analysis and assessed DNA damage in blood samples by the comet assay.RESULTS: The portal hypertension group exhibited increases in portal pressure when compared to SO group(29.8 ± 1.8 vs 12.0 ± 0.3 mm Hg)(P < 0.001). The same was observed when we compared the e NOS(56.8 ± 3.7 vs 13.46 ± 2.8 pixels)(P < 0.001),VEGF(34.9 ± 4.7 vs 17.46 ± 2.6 pixels)(P < 0.05),and NTT(39.01 ± 4.0 vs 12.77 ± 2.3 pixels)(P < 0.05) expression by immunohistochemistry of the PPVL animals with the SO group. The expression of e NOS(0.39 ± 0.03 vs 0.25 ± 0.03 a.μ)(P < 0.01) and VEGF(0.38 ± 0.04 vs 0.26 ± 0.04 a.μ)(P < 0.01) were also evaluated by Western blot analysis,and we observed an increase of both proteins on PPVL animals. We also evaluated the DNA damage by comet assay,and observed an increase on damage index and damage frequency on those animals. NAC decreased portal pressure values in PPVL + NAC animals(16.46 ± 2 vs 29.8 ± 1.8 mm Hg)(P < 0.001) when compared to PPVL. The expression of e NOS(14.60 ± 4.1 vs 56.8 ± 3.7 pixels)(P < 0.001),VEGF(19.53 ± 3.2 vs 34.9 ± 4.7 pixels)(P < 0.05) and NTT(21.84 ± 0.7 vs 39.01 ± 4.0 pixels)(P < 0.05) evaluated by immunohistochemistry were also reduced in PPVL + NAC animals. Also,when evaluated by Western blot e NOS expression(0.32 ± 0.03 vs 0.39 ± 0.03 a.μ)(P < 0.05) and VEGF expression(0.31 ± 0.09 vs 0.38 ± 0.04 a.μ)(P < 0.01). Furthermore,NAC modulated DNA damage in PPVL + NAC animals.CONCLUSION: In view of these results,we believe NAC is able to protect the stomach from the alterations induced by the PPVL procedure. | Francielli Licks Renata Minuzzo Hartmann Camila Marques Elizangela Schemitt Josieli Raskopf Colares Mariana do Couto Soares Juliana Reys Camila Fisher Juliana da Silva Norma Possa Marroni | 2015 | World Journal of Gastroenterology2015，21，43： | 2 |
| 2 | Pruritus as a symptom of hepatitis C显示文摘 | Fisher DA Wright TL | 1994 | J Am AcadDermatol1994，30，4： | 1 |
| 3 | Pathways for angiotensin Ⅱ generation in intact human tissue:evidence from comparative pharmacological interruption of the renin system显示文摘 | HOLLENBERG NK FISHER NDL PRICE DA | 1998 | Hypertension1998，32，3： | 1 |
| 4 | Bad design, bad practices, bad bugs: frustrations in controlling an outbreak of Elizabe- thkingia meningoseptica in intensive care units 显示文摘 | Balm MN Salmon S Jureen R Teo C Mahdi R Seetoh T Teo JT Lin RT Fisher DA | 2013 | J Hosp Infect2013，85，2： | 1 |
| 5 | Three Dact gene family members are expressed during embryonic development and in the a-dult brains of mice显示文摘 | Fisher DA Kivimae S Hoshino J | 2006 | Dev Dyn2006，235，9： | 1 |
| 6 | A randomized double-blind comparison of the effects of clomiphene citrate and the aromatase in- hibitor letrozole on ovulatory function in normal women显示文摘 | Fisher SA Reid RL Van Vugt DA | 2002 | Fertil Steril2002，78，2： | 1 |
| 7 | Small lntegrin-Binding Proteins as Serum Markers for Prostate Cancer Detection显示文摘 | Jain A McKnight DA Fisher LW | | 0，，16： | 1 |
| 8 | Histopathologic and biochemical changes in the muscles affected by distraction osteogenesis of the mandible显示文摘 | Fisher E Staffenberg DA McCarthy JG | 1997 | Plast Reconstr Surg1997，99，2： | 1 |
| 9 | Fetal thyroid function :diagnosis and management of fetal thyroid disorders 显示文摘 | Fisher DA | 1997 | Clin Obstet Gynecol1997，40，1： | 1 |
| 10 | Thyroidal radioiodine clearance and thyroid iodine accumulation:Contrast between random daily variation and population data显示文摘 | Fisher DA Oddie TH | 1969 | J Clin Endocrinol Metab1969，29，： | 1 |
| 11 | Thyroid iodine content and turnover in euthyroid subjects:Validity of estimation of thyroid iodine accumulation from shortterm clearance studies显示文摘 | Fisher DA Oddie TH | 1969 | J Clin Endocrinol Metab1969，29，： | 1 |
| 12 | Whole-rat condi- tional gene knockout via genome editing显示文摘 | Brown AJ Fisher DA Kouranova E | 2013 | Nat Meth- ods2013，10，7： | 1 |
| 13 | The hypothalamic -pituitary - thyroid negative feedback control axis in children with treated congeni- tal hypothyroidism 显示文摘 | Fisher DA Schoen EJ La Franchi S etal | 2000 | J Clin Endocrinol Metab2000，85，8： | 1 |
| 14 | BOLD MRI and early impairment of cerebrovascular reserve after aneurysmal subarachnoid hemorrhage显示文摘 | da Costa L Fierstra J Fisher JA | 2014 | J Magn Reson Imaging2014，40，4： | 1 |
| 15 | The diagxiosis of Hashimoto' s thyroiditis 显示文摘 | Fisher DA Oddie TH Johnoson DE etal | 1975 | Chin Endocrinol Metab1975，40，： | 1 |
| 16 | Thyroid system immaturities in very low birth weight premature infants 显示文摘 | Fisher DA | 2008 | Semin Perinatol2008，32，6： | 1 |
| 17 | Flexible structures of SIBLING proteins,bone sialoprotein,and osteopontin显示文摘 | Fisher LW Torchia DA Fohr B | | 0，，02： | 1 |
| 18 | Relation of visual function to retinal nerve fiber layer thickness in multiple sclerosis显示文摘 | Fisher JB Jacobs DA Markowitz CE | 2006 | Ophthalmology2006，113，2： | 1 |
| 19 | Flexible structures of SIBLING proteins, bone sialoprotein, and osteoponlin 显示文摘 | Fisher LW Torehia DA Fohr B | 2001 | Biochem Biophys Res Commun2001，280，2： | 1 |
| 20 | Fetal thyroid function:diagnosis and management of fetal thyroid disorders显示文摘 | Fisher DA | 1997 | Clin Obster Gynecol1997，1，40： | 1 |
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