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| 1 | Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells显示文摘AIM:To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells. METHODS: Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths. RESULTS: Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P < 0.05) adhesion to all the cell lines and clones tested, when compared to the non-pathogenic strain HPTx30a. Bacteria showed a significantly higher (P < 0.05) adhesion to the GP202 cell line, when compared to the MKN45 cell line. Furthermore, both strains showed a significantly higher (P < 0.05) adhesion to GP202 clones with larger MUC1 VNTR domains. CONCLUSION: This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells. The extent of bacterial adhesion depends on the size of theMUC1 VNTR domain. The adhesion is further dependent on bacterial pathogenicity and the gastric cell line. MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa. | Natália R Costa Nuno Mendes Nuno T Marcos Celso A Reis Thomas Caffrey Michael A Hollingsworth Filipe Santos-Silva | 2008 | World Journal of Gastroenterology2008，14，9： | 4 |
| 2 | Electrical spin injection and accumulation at room temperature in an all-metal mesoscopic spin valve显示文摘 | Filip A T van Wees B J | 2001 | Nature2001，410，： | 1 |
| 3 | Amino acids profile of Serbian unifloral honeys显示文摘 | Jelena K Jelena T Filip A | 2013 | Journal of the Science of Food and Agricuhure2013，93，13： | 1 |
| 4 | Identification of a novel group of bacteria phosphorus removal react-or显示文摘 | Nielsen A T Liu W T Filipe C | 1999 | Journal of Applied Microbiology1999，65，3： | 1 |
| 5 | Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor 显示文摘 | Nielsen A T Liu W T Filipe C | 1999 | Applied and Environmental Microbiology1999，65，3： | 1 |
| 6 | Identification of a novel group of bacterial in sludge from a deteriorated biological phosphorus removal reactor显示文摘 | Nielsen A T Liu W T Filipe C | 1999 | Appl Environ Microbiol1999，65，： | 1 |
| 7 | A new approach for devising local graph invariants:derived topological indices with low degeneracy and good correlational ability显示文摘 | Filip P A Balaban T S Balaban A T | | 0，，： | 1 |
| 8 | Identification of a novel group of bacterial in sludge from a deteriorated bio-logical phosphorus removal reactor显示文摘 | Nielsen A T Liu W T Filipe C et a1 | 1999 | Appl Environ Mierobiol1999，65，： | 1 |
| 9 | Identification of a novel group of bacterial in sludge from a deteriorated biological phosphorus removal reactor显示文摘 | NIELSEN A T W LIU T FILIPE C | 1999 | Appl Environ Microbiol1999，65，： | 1 |
| 10 | Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor显示文摘 | Nielsen A T Liu W T Filipe C | 1999 | Applied and Environmental Microbiology1999，65，3： | 1 |
| 11 | Synthesis and characterization of polymer/clay nanocomposites by interca- lated chain transfer agent显示文摘 | Hakan A Mehmet A T Filip D P | 2008 | Eur Potym J2008，，44： | 1 |
| 12 | Identification of A Novel Group of Bacteria in Sludge From a Deteriorated Biological Phosphorus Removal Reactor显示文摘 | NIELSEN A T LIU W T FILIPE C | 1999 | Appl Environ Microbiol1999，65，： | 1 |
| 13 | Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor 显示文摘 | NIELSEN A T LIU W T FILIPE C | 1999 | Appl Environ Microbiol1999，65，： | 1 |
| 14 | Electrical spin injection and accumulation at room temperature in an all-metal mesoscopic spin valve显示文摘 | Jedema F J Filip A T Van Wees B | 2001 | Nature2001，410，： | 1 |
| 15 | Electrical spin injection and accumulation in room temperature in all-metal mesoscopic spin valve显示文摘 | Jedema F J Filip A T Van Wees B | 2001 | Natrue2001，410，： | 1 |
| 16 | Performance of a yeast-me- diated biological fuel cell显示文摘 | GUNAWARDENA A G SANDUN F FILIP T | 2008 | Int J Mol Sci2008，9，： | 1 |
| 17 | Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor显示文摘 | NIELSEN A T LIU W T FILIPE C | 1999 | Applied and Environmental Microbiology1999，65，3： | 1 |
| 18 | Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor显示文摘 | Nielsen A T Liu W T Filipe C | | 0，，03： | 1 |
| 19 | Exosomal glypican-1 is elevated in pancreatic cancer precursors and can signal genetic predisposition in the absence of endoscopic ultrasound abnormalities显示文摘BACKGROUND Individuals within specific risk groups for pancreatic ductal adenocarcinoma(PDAC)[mucinous cystic lesions(MCLs),hereditary risk(HR),and new-late onset diabetes mellitus(NLOD)]represent an opportunity for early cancer detection.Endoscopic ultrasound(EUS)is a premium image modality for PDAC screening and precursor lesion characterization.While no specific biomarker is currently clinically available for this purpose,glypican-1(GPC1)is overexpressed in the circulating exosomes(crExos)of patients with PDAC compared with healthy subjects or those harboring benign pancreatic diseases.AIM To evaluate the capacity of GPC1+crExos to identify individuals at higher risk within these specific groups,all characterized by EUS.METHODS This cross-sectional study with a prospective unicentric cohort included 88 subjects:40 patients with MCL,20 individuals with HR,and 20 patients with NLOD.A control group(CG)was submitted to EUS for other reasons than pancreatic pathology,with normal pancreas and absence of hereditary risk factors(n=8).The inclusion period was between October 2016 and January 2019,and the study was approved by the Ethics Committee of Centro Hospitalar Universitário de São João,Porto,Portugal.All patients provided written informed consent.EUS and blood tests for quantification of GPC1+crExos by flow cytometry and carbohydrate antigen 19-9(CA 19-9)levels by ELISA were performed in all subjects.EUS-guided tissue acquisition was done whenever necessary.For statistical analysis,SPSS®27.0(IBM Corp.,Armonk,NY,United States)version was used.All graphs were created using GraphPad Prism 7.00(GraphPad Software,San Diego,CA,United States).RESULTS Half of MCLs harbored worrisome features(WF)or high-risk stigmata(HRS).Pancreatic abnormalities were detected by EUS in 10.0%and 35.0%in HR and NLOD individuals,respectively,all considered non-malignant and“harmless.”Median levels of GPC1+crExos were statistically different:MCL[99.4%,interquartile range(IQR):94.9%-99.8%],HR(82.0%,IQR:28.9%-98.2%),NLOD(12.6%,IQR:5.2%-63.4%),and CG(16.2%,IQR:6.6%-20.1%)(P<0.0001).Median levels of CA 19-9 were within the normal range in all groups(standard clinical cut-off of 37 U/mL).Within HR,individuals with a positive history of cancer had higher median levels of GPC1+crExos(97.9%;IQR:61.7%-99.5%),compared to those without(59.7%;IQR:26.3%-96.4%),despite no statistical significance(P=0.21).Pancreatic cysts with WF/HRS were statistically associated with higher median levels of GPC1+crExos(99.6%;IQR:97.6%-99.8%)compared to those without(96.5%;IQR:81.3%-99.5%)(P=0.011),presenting an area under the receiver operating characteristic curve value of 0.723(sensitivity 75.0%and specificity 67.7%,using a cutoff of 98.5%;P=0.012).CONCLUSION GPC1+crExos may act as biomarker to support the diagnosis and stratification of PDAC precursor lesions,and in signaling individuals with genetic predisposition in the absence of EUS abnormalities. | Pedro Moutinho-Ribeiro Ines A Batista Sofia T Quintas Bárbara Adem Marco Silva Rui Morais Armando Peixoto Rosa Coelho Pedro Costa-Moreira Renato Medas Susana Lopes Filipe Vilas-Boas Manuela Baptista Diogo Dias-Silva Ana L Esteves Filipa Martins Joanne Lopes Helena Barroca Fátima Carneiro Guilherme Macedo Sonia A Melo | 2022 | World Journal of Gastroenterology2022，28，31： | 0 |
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