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| 1 | Liver-specific gene expression in mesenchymal stem cells is induced by liver cells显示文摘AIM: The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stem cell cultures. We investigated the potential of rat mesenchymal stem cells (MSC) from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore,we assessed the influence of cocultured liver cells on induction of liver-specific gene expression.METHODS: Mesenchymal stem cells were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF,EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thy1 and the hepatocytic markers CK-18, albumin, CK-19,and AFP was performed in the different cell populations.RESULTS: Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18,CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression.CONCLUSION: The results indicate that (1) rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and (2) that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC. | Claudia Lange Philipp Bassler Michael V. Lioznov Helge Bruns Dietrich Kluth Axel R. Zander Henning C. Fiegel | 2005 | World Journal of Gastroenterology2005，11，29： | 31 |
| 2 | Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells显示文摘瞄准:为了与胎儿的肝细胞(FLC ) 和可能性在合作文化调查间充质的干细胞(MSC ) 的 hepatocytic 区别膨胀,区分了 hepatocytic 房间。方法:MSC 被制动火箭与绿荧光灯的蛋白质(GFP ) 标记病毒的基因转导变异。同种细胞的显著 MSC 在用与干细胞补充的fibronectin涂的培养皿和媒介刺激条件的肝下面是也有教养的因素( SCF ), hepatocyte 生长因素( HGF ),表皮的生长因素( EGF ),和成纤维细胞生长因素 4 ( FGF-4 )独自一个,或在刚孤立的 FLC 的存在。在合作文化的房间被收获,并且 GFP+ 或 GFP- 房间用荧光被分开激活的房间排序。为肝 specific 标记 cytokeratin-18 (CK-18 ) 的反向的抄写聚合酶链反应(RT-PCR )( 法新社) ,白朊,和 alpha-fetoprotein 在不同房间人口被执行。结果:在指定文化条件下面,与 FLC co 有教养的老鼠 MSC 超过二个星期表示了白朊, CK-18,和 AFP-RNA。在 wk 3, MSC 失去了 hepatocytic 基因表示,可能由于 cocultured FLC 的增生。FLC 也在合作文化和一个很高的生长潜力显示出稳定的肝 specific 基因表情。结论:从骨髓的老鼠 MSC 能面对 FLC 在试管内区分 hepatocytic 房间,在合作文化的 MSC 的存在也为 FLC 的扩大和区别提供有益的环境。 | Claudia Lange Helge Bruns Dietrich Kluth Axel R Zander Henning C Fiegel | 2006 | World Journal of Gastroenterology2006，12，15： | 23 |
| 3 | Differential changes in intrinsic innervation and interstitial cells of Cajal in small bowel atresia in newborns显示文摘AIM: To investigate morphological changes of the enteric nervous system (ENS) and the interstitial cells of Cajal (ICCs) in small bowel atresia.METHODS: Resected small bowel specimens from affected patients (n = 7) were divided into three parts (proximal, atretic, distal). Standard histology and enzyme immunohistochemistry anti-S100, anti-protein gene product (PGP) 9.5, anti-neurofilament (NF), antic-kit-receptor (CD117) was carried out on conventional paraffin sections of the proximal and distal part. RESULTS: The neuronal and glial markers (PGP 9.5, NF, S-100) were expressed in hypertrophied ganglia and nerve fibres within the myenteric and submucosal plexuses. Furthermore, the submucous plexus contained typical giant ganglia. The innervation pattern of the proximal bowel resembled intestinal neuronal dysplasia. The density of myenteric ICCs was clearly reduced in the proximal bowel, whereas a moderate number of muscular ICCs were found. The anti-CD117 immunore- action revealed additional numerous mast cells. The distal bowel demonstrated normal morphology and density of the ENS, the ICCs and the mast cells.CONCLUSION: The proximal and distal bowel in small bowel atresia revealed clear changes in morphology and density of the ENS and ICCs. | Stefan Gfroerer Roman Metzger Henning Fiegel Priya Ramachandran Udo Rolle | 2010 | World Journal of Gastroenterology2010，16，45： | 9 |
| 4 | Complications of newborn enterostomies显示文摘AIM To evaluate the occurrence and severity of enterostomy complications in newborns suffering from different intestinal disorders.METHODS A 10-year retrospective cohort study(2008-2017) investigated newborns that underwent enterostomy formation and reversal for different intestinal disorders. Only infants less than 28 d old at the time of enterostomy creation were included in the study(corrected age was applied in the cases of preterm neonates). The patients were divided into two groups according to their underlying diseases. Group 1 included infants suffering from necrotizing enterocolitis(NEC), whereas Group 2 included newborns diagnosed with intestinal disorders other than NEC, such as meconium obstruction, anorectal malformation, focal intestinal perforation, ileus, intestinal atresia and volvulus. The primary outcome measure was enterostomy-related morbidity. The data were analyzed statistically using Pearson's χ2 test or Fisher's exact test for categorical variables and the Wilcoxon-Mann-Whitney U-Test for continuous variables. RESULTS In total, 76 infants met the inclusion criteria and were evaluated for enterostomy-related complications. Neither group showed significant differences regarding gender, gestational age, weight at birth or weight at enterostomy formation. Infants suffering from NEC(Group 1) were significantly older at enterostomy for-mation than the neonates of Group 2 [median(range), 11(2-75) d vs 4(1-101) d, P = 0.004)]. Significantly more ileostomies were created in Group 1 [47(92.2%) vs 16(64.0%), P = 0.007], whereas colostomies were performed significantly more often in Group 2 [2(3.9%) vs 8(32.0%), P = 0.002]. The initiation of enteral nutrition after enterostomy was significantly later in Group 1 infants than in Group 2 infants [median(range), 5(3-13) vs 3(1-9), P < 0.001]. The overall rate of one or more complications in patients of both groups after enterostomy formation was 80.3%, with rates of 86.3% in Group 1 and 68.0% in Group 2(P = 0.073). Most patients suffered from two complications(23.7%). Four or more complications occurred in 21.6% of the infants in Group 1 and in 12.0% of the infants in Group 2(P = 0.365). Following enterostomy closure, at least one complication was observed in 26.0% of the patients(30.6% in Group 1 and 16.7% in Group 2, P = 0.321). The occurrence of complications was not significantly different between neonates with NEC and infants with other intestinal disorders. 48(65.8%) patients required no treatment or only pharmacological treatment for the complications that occurred [Clavien-Dindo-Classification(CDC) < Ⅲ], while 25(34.2%) required surgery to address the complications(CDC ≥Ⅲ). Early reversal of the enterostomy was performed significantly more often(P = 0.003) and the time to full enteral nutrition after closure was significantly longer [median(range), 7(3-87) d vs 12(5-93) d, P = 0.006] in infants with a CDC grading ≥Ⅲ than in infants with a CDC grading < Ⅲ. CONCLUSION Complications occur in almost all infants with enterostomies. The majority of these complications are minor and do not require surgical treatment. There is a clear trend that neonates with NEC have a higher risk for developing complications than those without NEC. | Lea Wolf Stefan Gfroerer Henning Fiegel Udo Rolle | 2018 | World Journal of Clinical Cases2018，6，16： | 5 |
| 5 | Abdominal cocoon in children: A case report and review of literature显示文摘BACKGROUND Abdominal cocoon or“encapsulating peritoneal sclerosis”(EPS)is an uncommon and rare cause of intestinal obstruction.Only a few cases have been reported in paediatric patients.Typically,EPS is described as the primary form in young adolescent girls from tropical and subtropical countries because of viral peritonitis due to retrograde menstruation or a history of peritoneal dialysis.Most patients are asymptomatic or present with abdominal pain,which is likely to occur secondary to subacute bowel obstruction.Findings at imaging,such as ultrasound,computed tomography,and magnetic resonance imaging,are often nonspecific.When diagnosed,EPS is characterized by total or partial encasement of the bowel within a thick fibrocollagenous membrane that envelopes the small intestine in the form of a cocoon because of chronic intraabdominal fibroinflammatory processes.The membrane forms a fibrous tissue sheet that covers,fixes,and finely constricts the gut,compromising its motility.CASE SUMMARY We present a case of EPS in a 12-year-old boy 8 wk after primary surgery for resection of symptomatic jejunal angiodysplasia.There was no history of peritoneal dialysis or drug intake.CONCLUSION In this report,we sought to highlight the diagnostic,surgical,and histopathological characteristics and review the current literature on EPS in paediatric patients. | Daniel Keese Andrea Schmedding Kerstin Saalabian Georgy Lakshin Henning Fiegel Udo Rolle | 2021 | World Journal of Gastroenterology2021，27，37： | 2 |
| 6 | Wound healing angiogenesis:Indirect stimulation by bFGF 显示文摘 | Kinghton DR Phillips GD Fiegel VD | 1990 | J Trauma1990，30，12： | 1 |
| 7 | An angiogenic extract from skeletal muscle stimulates monoeyte and endothelial cell chemotaxis in vitro显示文摘 | Phillips G D Sehilb L A Fiegel V D | 1991 | Proe Soe Exp Biol Med1991，197，4： | 1 |
| 8 | Characterization of cell types during rat liver development显示文摘 | Park JJ Lioznov MV | 2003 | Hepatology2003，37，1： | 1 |
| 9 | Angiogenic extract from skeletal muscle stimulates monoeyte and endothelial cell chemotaxis in vitro显示文摘 | Phillips GD Schilb LA Fiegel VD | 1991 | Proc Soc Exp Biol Med1991，197，： | 1 |
| 10 | Long-term differentiated function of heterotopically transplanted hepatocytes on threedimensional polymer matrices显示文摘 | Kneser U Kaufmann PM Fiegel HC | 1999 | J Biomed Mater Res1999，47，4： | 1 |
| 11 | Liver-Specific gene expression in cultured human hematopoietie stem cell显示文摘 | FIEGEL H C LIOZNOV M V CORTES DERICKS L | 2003 | Stem Ceils2003，21，1： | 1 |
| 12 | Influence of flow conditions and matrix coatings on growth and differentiation of three- dimensionally cultured rat hepatocytes显示文摘 | Fiegel HC Havers J Kneser U | 2004 | Tissue Eng2004，10，12： | 1 |
| 13 | Long- term differentiated function of heterotopically transplanted bepatocytes on three - dimensional polymer matrices显示文摘 | Kneser U Knufmann PM Fiegel HC | 1999 | J Biomed Mater Res1999，47，3： | 1 |
| 14 | Liquefaction mechanism for layered soils显示文摘 | FIEGEL G L KUTTER B L | 1994 | Journal of Geotechnical Engineering ASCE1994，120，4： | 1 |
| 15 | Stem-like cells in human hepatoblastoma 显示文摘 | Fiegel H C Gluer S Roth B | 2004 | Histochem Cytochem2004，52，11： | 1 |
| 16 | Angiogenic extract from skeletalmuscl e stimulates monocyte and endothelial cell chemotaxis in vitro显示文摘 | Philips GD Schilb LA Fiegel VD | 1991 | Proc Sdc Exp Biol Med’1991，197，3： | 1 |
| 17 | Fetal and adult liver stem cells for liver regeneration and tissue engineering 显示文摘 | Fiegel HC Lange C Kneser U | 2006 | J Cell Mol Med2006，10，3： | 1 |
| 18 | Fetal and adult liver stem cells for liver regeneration and tissue engineering显示文摘 | Fiegel HC Lange C Kneser U | 2006 | J Cell Mol Med2006，10，3： | 1 |
| 19 | Airborne infectious disease and the suppression of pulmonary bioaerosols 显示文摘 | FIEGEL J CLARKE R EDWARDS D A | 2006 | Drug Disorery Today2006，11，12： | 1 |
| 20 | Characterization of cell types during rat liver development显示文摘 | Fiegel HC Park JJ | | 0，，01： | 1 |
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