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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Role of cytochrome P450 2E1 in the metabolism of acrylamide and acrylonitrile in mice显示文摘 | S C Sumner T R Fennell T A Moore | 1999 | Chem Res Toxicol1999，12，： | 1 |
| 3 | Bone graft and bone substi- tutes 显示文摘 | Schachar N Fennel C Otsuke T | 2002 | Orthopaedics Philadelphia Mosby2002，，： | 1 |
| 4 | Benign calcaneal bone cyst and pathologic fracture-surgical treatment with injectable calcium-phosphate bone cement ( Norian ): a case report 显示文摘 | Csizy M Buckley RE Fennell C | 2001 | Foot Ankle Iht2001，22，6： | 1 |
| 5 | Oxidative stress and vas- cular damage in hypertension 显示文摘 | Berry C Brosman M J Fennell J | 2001 | Curr Opin Nephrol Hypertens2001，10，： | 1 |
| 6 | Internal Charging: a Preliminary Environmental Specification for Satellites显示文摘 | FENNELL J F KOONS H C CI-IEN M W | 2000 | Ieee Trans Plasma Sci2000，28，6： | 1 |
| 7 | Measuring the Ethical Nature of Tourism Operators 显示文摘 | David A Fennell David C Malloy | 1999 | Annals of Tourism Research1999，，26： | 1 |
| 8 | Codes of ethics and tourism: An exploratory content analysis 显示文摘 | David C Malloy David A Fennell | 1998 | Tourism Management1998，，19： | 1 |
| 9 | Internal Charging: A Preliminary Environmental Specification for Satellites显示文摘 | Fennell J F Koons H C Chen M W Blake J B | 2000 | IEEE TRANSACTIONS ON PLASMA SCIENCE2000，28，6： | 1 |
| 10 | Gentamicin in Tissue and Whole Milk : An Improved Method for Extraction and Cleanup of Samples for Quantitation on HPLC 显示文摘 | Fennell M A Uboh C E Sweeney R W | 1995 | J Agric Food Chem1995，43，： | 1 |
| 11 | Use of 1H-NMR to determine grape bud water state during the photoperiodic induction of dormancy 显示文摘 | Fennell A Wake C Molitor P | 1996 | Amer Soc Hort Sci1996，121，： | 1 |
| 12 | Early words learners ability to access phonetic detail in well-known words显示文摘 | FENNEL C T WERKER J F | 2003 | Language and Speech2003，，46： | 1 |
| 13 | Validation of a low-cost commercially-available accelerometer during low and high physical activity conditions in children 显示文摘 | Fennell C Kobak M Glickman EL | 2016 | Medicine & Science in Sports & Exercise2016，48，5： | 1 |
| 14 | Thymidylate Syn- thase Expression and Outcome of Patients Receiving Pemetrexed for Advanced Nonsquamous Non-Small- Cell Lung Cancer in a Prospective Blinded Assessment Phase II Clinical Trial显示文摘 | Nicolson Marianne C Fennell | 2013 | Journal of Thoracic Oncolo- gy2013，8，7： | 1 |
| 15 | Characterization and quantitation of urinary metabolites of acrylamide in ratsand mice using 13C nuclear magnetic resonance spectroscopy显示文摘 | Sumner S C J MacNeela J P Fennel T R | 1992 | Chem Res Toxieol1992，5，1： | 1 |
| 16 | Urovirulence determinants in escherichia coil stains causing prostatitis显示文摘 | Andreu A Stapleton AE Fennell C | 1997 | J Infect Dis1997，176，1： | 1 |
| 17 | Charecterization of two plasmids from Campylobacter jejuni isolates that carry the aphA-7 kanamycin resistance determinant显示文摘 | Tenover F C Fennell C L Lee L | 1992 | Antimicrob Agents Chemother1992，36，4： | 1 |
| 18 | Semitubular blade platefixation in proximal humeral fractures:a biomechanical study in acadaveric model显示文摘 | Instrum K Fennell C Shrive N | 1998 | J Shoulder Elbow Surg1998，7，： | 1 |
| 19 | Comparison of different natural sorbents for removing CO2 from combustion gases, as studied in a bench-scale fuidized bed显示文摘 | Ives M Mundy R C Fennell P S | 2008 | Energy & Fuels2008，22，6： | 1 |
| 20 | Comparison of different natural sorbents for removing CO2 from combustion gases,as studied in a bench-scale fluidized bed显示文摘 | Ives M Mundy R C Fennell P S Davidson J F Dennis J S Hayhurst A N | 2008 | Energy Fuels2008，22，6： | 1 |
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