|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries显示文摘Due to the limitations in autogenous nerve grafting or Schwann cell transplantation,large gap peripheral nerve injuries require a bridging strategy supported by nerve conduit.Cell based therapies provide a novel treatment for peripheral nerve injuries.In this study,we first experimented an optimal scaffold material synthesis protocol,from where we selected the 10%GFD formula(10%GelMA hydrogel,recombinant human basic fibroblast growth factor and dental pulp stem cells(DPSCs))to fill a cellulose/soy protein isolate composite membrane(CSM)tube to construct a third generation of nerve regeneration conduit,CSM-GFD.Then this CSM-GFD conduit was applied to repair a 15-mm long defect of sciatic nerve in a rat model.After 12 week post implant surgery,at histologic level,we found CSM-GFD conduit could regenerate nerve tissue like neuron and Schwann like nerve cells and myelinated nerve fibers.At physical level,CSM-GFD achieved functional recovery assessed by a sciatic functional index study.In both levels,CSM-GFD performed like what gold standard,the nerve autograft,could do.Further,we unveiled that almost all newly formed nerve tissue at defect site was originated from the direct differentiation of exogeneous DPSCs in CSM-GFD.In conclusion,we claimed that this third-generation nerve regeneration conduit,CSM-GFD,could be a promising tissue engineering approach to replace the conventional nerve autograft to treat the large gap defect in peripheral nerve injuries. | Lihua Luo Yan He Ling Jin Yanni Zhang Fernando P.Guastaldi Abdullkhaleg A.Albashari Fengting Hu Xiaoyan Wang Lei Wang Jian Xiao Lingli Li Jianming Wang Akon Higuchi Qingsong Ye | 2021 | Bioactive Materials2021，6，3： | 11 |
| 2 | Phylogenomic tracing of asymptomatic transmission in a COVID-19 outbreak显示文摘SARS-CoV-2 has caused over 100 million deaths and continues to spread rapidly around the world.Asymptomatic transmission of SARS-CoV-2 is the Achilles’heel of COVID-19 public health control measures.Phylogenomic data on SARS-CoV-2 could provide more direct information about asymptomatic transmission.In this study,using a novel MINERVA sequencing technology,we traced asymptomatic transmission of COVID-19 patients in Beijing,China.One hundred and seventy-eight close contacts were quarantined,and 14 COVID-19 patients were laboratory confirmed by RT-PCR.We provide direct phylogenomic evidence of asymptomatic transmission by constructing the median joining network in the cluster.These data could help us to determine whether the current symptom-based screening should cover asymptomatic persons. | Ju Zhang Nan Ding Yangzi Song Rui Song Yang Pan Linghang Wang Shuo Yan Qi Wang Shanfang Ma Lirong Wei Fengting Yu Lianhe Lu Fujie Zhang Chen Chen Hui Zeng | 2021 | The Innovation2021，2，2： | 1 |
| 3 | Strong Inverse Correlation Between MicroRNA-125b and Human Papillomavirus DNA in Productive Infection显示文摘 | Gerard J. Nuovo Xin Wu Stefano Volinia Fengting Yan Gianpiero di Leva Nena Chin Alcina F. Nicol Jinmai Jiang Gregory Otterson Thomas D. Schmittgen Carlo Croce | 2010 | Diagnostic Molecular Pathology2010，，3： | 1 |
| 4 | Self-synthesized second mitochondria-derived activator of caspase(SMAC)mimetic TP-WY-1345 enhances the radiosensitivity of NSCLC cells H1299 by targeting anti-apoptotic protein cIAP1显示文摘Objective:To explore the toxicity and cellular uptake of the self-synthesized second mitochondria-derived activator of caspase(SMAC)mimetic TP-WY-1345 polypeptide and its radiosensitizing effect on non-small cell lung cancer(NSCLC).Methods:A fluorescence microscope was used to observe the uptake efficiency of TP-WY-1345 by human NSCLC cells H1299.Toxicity of the TP-WY-1345 peptide in normal cells was examined in human embryonic lung fibroblasts MRC5.CCK-8 and clone formation experiments were performed to detect the radiosensitizing effect of TP-WY-1345 in the H1299 cells.The AutoDock Vina simulation software was employed to predict the binding efficiency of TP-WY-1345 to the inhibitor of apoptosis proteins(IAPs).Moreover,Western blot and qPCR experiments were carried out to determine the protein and gene expressions,and the flow cytometry(FCM)technique was used to detect the apoptosis level under different conditions.Results:TP-WY-1345 can be self-synthesized,and significant green fluorescence was observed in H12992 h and 6 h after incubation with TP-WY-1345.The cell counting and CCK-8 results showed that 10μmol/L and 20μmol/L TP-WY-1345 did not produce a significant toxic effect on the MRC5 cells(P>0.05).Compared with the single ionizing radiation group,the cell viability and clone formation of H1299 cells were significantly inhibited after treatment with TP-WY-1345 at a concentration of 50μmol/L(P<0.05).The sensitivity enhancement ratio(SER)was calculated to be 1.14.Moreover,the binding efficiency of TP-WY-1345 to cIAP1 protein was predicted to be7.3,and this strong binding force was demonstrated by Western blot.TP-WY-1345 inhibited the protein and mRNA expressions of cIAP1 and further increased the apoptosis level of the H1299 cells at 24 h(P<0.01)and 48 h after radiation(P<0.05).Conclusion:SMAC mimetic TP-WY-1345 can enter the H1299 cells and produce a radiosensitizing effect by increasing the level of radiation-induced apoptosis of the cells.There,TP-WY-1345 is expected to become a new generation of radiosensitizing drugs. | Hao Sun Fengting Liu Hezheng Zhai Jiang Wu Shasha Nie Hui Cai Kaixue Wen Li Feng Qiang Liu Kaihua Ji Yan Wang | 2023 | Radiation Medicine and Protection2023，4，1： | 0 |
| 5 | Simulation of Passenger Behavior in Virtual Subway Station Based on Multi-Agent显示文摘According to architectural structure of underground station and condition of passengers,the emergency evacuations are extremely important during emergency accidents caused in the stations.Based on the architectural features and situation of passengers in the station,Multi-Agent system is proposed to analyze passengers’behavior and elements which affect passengers’behavior.Base on the crowd mentality,the model of selective behavior of passengers’route was established to simulate passengers’actions during emergency accidents.It is feasible to find out the influence brought by crowd mentality affect the emergency evacuations,and the influence of remitting the situation from external factor through WebVR.At last,it provides references for emergency evacuation strategy. | Yan Li Zequn Li Fengting Yan Yu Wei Linghong Kong Zhicai Shi Lei Qiao | 2020 | 国际计算机前沿大会会议论文集2020，，2： | 0 |
| 6 | Corrigendum to‘Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries’[Bioactive Mater.6(3)(2021)638-654]显示文摘The authors regret that the author(A.H.)was removed as an agreement could not be reached between the two affiliations of the author.The authors would like to apologise for any inconvenience caused. | Lihua Luo Yan He Ling Jin Yanni Zhang Fernando P.Guastaldi Abdullkhaleg A.Albashari Fengting Hu Xiaoyan Wang Lei Wang Jian Xiao Lingli Li Jianming Wang Akon Higuchi Qingsong Ye | 2022 | Bioactive Materials2022，7，1： | 0 |
| 7 | Assessing productivity and carbon sequestration capacity of subtropical coniferous plantations using the process model PnET-CN显示文摘概括的 A,块参数生态的模型 PnET-CN 在南部的中国为副热带的具球果的种植园被校准并且验证。PnET-CN 模型描述碳(C) 和氮(N) 和罐头的周期在估计碳隐遁潜力帮助的 biogeochemical。为 PnET-CN 的确认,从在南部的中国的具球果的森林种植园的数据被使用。从 2005 ~ 2007 的粗野主要生产率(GPP ) 与观察同意了很好的模仿的日报(R2=0.56, S.D.=0.009 ) 。从 20052007 的每月的土壤呼吸(R s ) 的模拟与 R s 观察同意了很好(R2=0.67, S.D =0.03 ) 。模仿的年度网从 19982006 的主要生产率(NPP ) 是 803 ???? ??????? ? | YAN Yuan WANG Shaoqiang WANG Yidong WU Weixing WANG Jingyuan CHEN Bin YANG Fengting | 2011 | Journal of Geographical Sciences2011，21，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员