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| 1 | Experimental study on the bolt–cable combined supporting technology for the extraction roadways in weakly cemented strata显示文摘Aiming at the characteristics of the poor steady ability, the short stable time and severe deformation behavior of weakly cemented soft surrounding rock around extraction roadway, a bolt–cable combined supporting technology was proposed. Numerical simulation was performed by using FLAC3 D software to study the effects of different supporting systems. The simulation result proves that those supporting systems have good practical values. Based on real-time monitoring and analysis of the deformation of surrounding rock and the stress of supporting structure, real time information of deformation of surrounding rock and stress state of supporting structure of extraction roadway within weakly cemented strata was obtained. Monitoring results show that large deformation and failure of surrounding rock of extraction roadway within weakly cemented strata can be effectively controlled by the bolt–cable combined supporting technology, which ensures the long-term stability and safety of surrounding rock and supporting structure. | Meng Qingbin Han Lijun Sun Jingwu Min Fengqing Feng Wei Zhou Xing | 2015 | International Journal of Mining Science and Technology2015，25，1： | 13 |
| 2 | Utilization of Ogura CMS germplasm with the clubroot resistance gene by fertility restoration and cytoplasm replacement in Brassica oleracea L显示文摘Clubroot disease,a major plant root disease caused by Plasmodiophora brassicae,has become one of the most destructive diseases among cultivated cruciferous vegetables.However,clubroot-resistant Brassica oleracea materials are rare.A few clubroot-resistant cabbage varieties are available on the market,but all are Ogura cytoplasmic male sterile(CMS)types.Therefore,in this study,to reutilize the clubroot-resistant Ogura CMS germplasm of cabbage,a new fertility-restored Ogura CMS material,16Q2-11,was used as a bridge to transfer the clubroot resistance(CR)gene from the Ogura CMS cytoplasm to the normal cytoplasm by a two-step method(a fertility restoration and cytoplasm replacement method).In the first cross for fertility restoration of Ogura CMS clubroot-resistant cabbage(FRCRC),16Q2-11 was used as a restorer to cross with Ogura CMS materials containing the CR gene CRb2.Eleven Rfo-positive progenies were generated,of which four contained CRb2:F8-514,F8-620,F8-732 and F8-839.After inoculation with race 4 of P.brassicae,these four CRb2-positive individuals showed resistance.Furthermore,F8-514 and F8-839 were then used as male parents in the second cross of FRCRC to cross with cabbage inbred lines,resulting in the successful introgression of the CRb2 gene into the inbred lines.All offspring produced from this step of cross,which had a normal cytoplasm,showed a high resistance to race 4 of P.brassicae and could be utilized for the breeding of clubrootresistant cabbage varieties in the future.This is the first time that the Ogura CMS restorer has been used to restore the fertility of Ogura CMS clubroot-resistant cabbages,which could improve germplasm diversity in cabbage and provide a reference method for using CMS germplasm in Brassica crops. | Wenjing Ren Zhiyuan Li Fengqing Han Bin Zhang Xing Li Zhiyuan Fang Limei Yang Mu Zhuang Honghao Lv Yumei Liu Yong Wang Hailong Yu Yangyong Zhang | 2020 | Horticulture Research2020，7，1： | 7 |
| 3 | Optically stimulated luminescence dating of sandy deposits from Gulang county at the southern margin of the Tengger Desert, China显示文摘Aeolian deposits from the deserts in northern China have been used for palaeoenvironmental research to understand aeolian sedimentology and its dynamic connection to past climate conditions. The Tengger Desert in China is sensitive to the waxing and waning of the monsoonal system. In response to past climate change, the southern margin of the Tengger Desert has evolved significantly since the last glacial period. However, previous attempts to date aeolian deposits in this region were mainly based on radiocarbon dating, which has problems when applied to aeolian deposits. Moreover, sedimentary records are limited. Accordingly, past aeolian activity in this desert remains poorly understood. In the present study, we dated sand samples from Gulang county at the southern margin of the Tengger Desert using optically stimulated luminescence(OSL) to understand the history of aeolian activity in this region. Our samples represented well-sorted aeolian sands and sandy loess. Aeolian sands are evidence of dune field buildup and sparse vegetation cover whereas sandy loess is evidence of improved stabilization of sand dunes resulting from ameliorated vegetation cover. Certain samples showed a decline in the equivalent dose(De) values when successive integration intervals were applied, which resulted from unstable OSL signals from non-fast components in the initial part of the decay curve. In order to obtain reliable De estimates, we investigated component-resolved and different background subtraction approaches, and compared the resultant De estimates. We adopted the early background subtraction method to derive De values. Luminescence chronologies and sedimentary records indicated that sand dunes accumulation occurred before 10 ka, and sandy loess developed between 9.5 and 7.6 ka when sand dunes were stabilized as a result of increased effective moisture levels. The transition between sand dune mobilization and stabilization emphasizes the significance of an effective moisture threshold in controlling aeolian activity. Mobilization of sand dunes at ~2.3 ka might be related to an increased aridity during the Late Holocene. | PENG Jun DONG Zhibao HAN Fengqing | 2016 | Journal of Arid Land2016，8，1： | 7 |
| 4 | An assessment and comparison of 230 Th and AMS 14 C ages for lacustrine sediments from Qarhan Salt Lake area in arid western China显示文摘 | QiShun Fan HaiZhou Ma ZhiBang Ma HaiCheng Wei FengQing Han | 2014 | Environmental Earth Sciences2014，，3： | 1 |
| 5 | Map-based cloning and CRISPR/Cas9-based editing uncover BoNA1 as the causal gene for the no-anthocyanin-accumulation phenotype in curly kale (Brassica oleracea var. sabellica)显示文摘Brassica oleracea comprises several important vegetable and ornamental crops,including curly kale,ornamental kale,cabbage,broccoli,and others.The accumulation of anthocyanins,important secondary metabolites valuable to human health,in these plants varies widely and is responsible for their pink to dark purple colors.Some curly kale varieties lack anthocyanins,making these plants completely green.The genetic basis of this trait is still unknown.We crossed the curly kale inbred line BK2019(without anthocyanins)with the cabbage inbred line YL1(with anthocyanins)and the Chinese kale inbred line TO1000(with anthocyanins)to generate segregating populations.The no-anthocyanin trait was genetically controlled by a recessive gene,bona1.We generated a linkage map and mapped bona1 to a 256-kb interval on C09.We identified one candidate gene,Bo9g058630,in the target genomic region;this gene is homologous to AT5G42800,which encodes a dihydroflavonol-4-reductase-like(DFR-like)protein in Arabidopsis.In BK2019,a 1-bp insertion was observed in the second exon of Bo9g058630 and directly produced a stop codon.To verify the candidate gene function,CRISPR/Cas9 gene editing technology was applied to knock out Bo9g058630.We generated three bona1 mutants,two of which were completely green with no anthocyanins,confirming that Bo9g058630 corresponds to BoNA1.Different insertion/deletion mutations in BoNA1 exons were found in all six of the other no-anthocyanin kale varieties examined,supporting that independent disruption of BoNA1 resulted in no-anthocyanin varieties of B.oleracea.This study improves the understanding of the regulation mechanism of anthocyanin accumulation in B.oleracea subspecies. | Kaiwen Yuan Xinyu Zhao Wenru Sun Limei Yang Yangyong Zhang Yong Wang Jialei Ji Fengqing Han Zhiyuan Fang Honghao Lv | 2023 | Horticulture Research2023，10，8： | 0 |
| 6 | Genetic diversity and population structure analysis of 161 broccoli cultivars based on SNP markers显示文摘To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on single nucleotide polymorphism(SNP)markers.Ten pairs of primers with good polymorphism and high resolution were screened from 315 pairs of SNP primers by 3 broccoli accessions(inbred lines)with different phenotypes and maturity.The 10 pairs of SNP primers were selected,producing 78 alleles.The diversity analysis indicated that the polymorphism information content(PIC)of SNP primer ranged from 0.64 to 0.90.The observed number of alleles(Na)was 2.00,the effective number of alleles(Ne)was 1.11–2.00,the Nei’s gene diversity(H)was 0.10–0.50,and Shannon information index(I)was 0.20–0.70 using PopGene32 software.The clustering results showed that the 161 broccoli cultivars could be divided into 4 major subgroups(A,B,C and D),foreign cultivars were all assigned to subgroup A,and domestic cultivars were assigned to 3 subgroups of B,C,and D.This study indicated that some domestic cultivars and foreign cultivars were similar in genetic background,but most domestic cultivars were still different from the Japanese cultivars.When K=2,the population structure result presented that 161 broccoli cultivars could be divided into 1 simple group(2 groups)and 1 mixed group.When Q≥0.6,143(88.82%)broccoli cultivars belonged to the simple groups.In simple groups 68(42.24%)broccoli cultivars of group 1 were derived from Japan,the United States,Switzerland,the Netherlands,China-Taiwan,and China-Mainland;75(46.58%)broccoli cultivars belonged to group 2;when Q<0.6,18(11.18%)broccoli cultivars belonged to the mixed groups.This study is helpful to understand the diversity and resolution of broccoli cultivars from worldwide,which is beneficial to plant breeding and materials innovation.And meanwhile,this result is also used for construction of broccoli fingerprint serving for cultivar identification. | Jingjing Huang Yumei Liu Fengqing Han Zhiyuan Fang Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Yong Wang Jialei Ji Zhansheng Li | 2021 | Horticultural Plant Journal2021，7，5： | 0 |
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