6篇
您的检索式：作者名="Feng Guohe"
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| 1 | Microelement analysis of source of raw materials of terracotta warriors and horses of Qin's Mausoleum in pits No.1 and No.2显示文摘In order to search for sources of the raw material of the Qin’s terracotta warriors and horses, we selected 20 samples of pottery warriors and pottery horses of the Qin’s Mausoleum from pit No. 1,19 samples of pottery warriors and brick from pit No. 2, 20 samples of clays near the Qin’s Mausoleum and 2 samples of Yaozhou porcelain bodies. The 32 kinds of element content of each sample were measured by neutron activation analysis (NAA). These data were analyzed by fuzzy cluster analysis, the trend cluster analysis diagram was obtained. The results showed that samples of terracotta warriors and horses from pits No.1 and No. 2 were closely related to the loam layer near Qin’s Mausoleum, but their relations with the loess layers were not so close, and they were out of all relation to Yaozhou porcelain bodies. Thus, it could be deduced that the Li Mountain clay may be considered as the original place of the raw materials of the terracotta warriors and horses, the kiln sites might be located nearby the Qin’s | Weijuan Zhao Aijun Xu Rongwu Li Zhengyao Gao Guoxia Li Jianzhong Xie Guohe Han Songlin Feng Dongyu Fan Ying Zhang et al. | 2002 | Chinese Science Bulletin2002，47，16： | 6 |
| 2 | Analysis of Airlines Marketing Based on Double Clustering显示文摘 | Feng Guohe | 2013 | 电子商务学刊(中英文版)2013，2，2： | 2 |
| 3 | Characterization and E protein expression of mutant strains during persistent infection of KN73 cells with Japanese encephalitis virus显示文摘 | Feng Guohe Tsutomu Takegami ZHAO Guizhen | | 0，，09： | 1 |
| 4 | Enhancedinterval linear progzamming显示文摘 | ZHOU Feng HUANG Guohe CHEN Guoxian | 2009 | European Journal of Operational Research2009，199，2： | 1 |
| 5 | Switch of substrate specificity of hyperthermophilic acylaminoacyl peptidase by combination of protein and solvent engineering显示文摘The inherent evolvability of promiscuous enzymes endows them with great potential to be artificially evolved for novel functions.Previously,we succeeded in transforming a promiscuous acylaminoacyl peptidase(apAAP)from the hyperthermophilic archaeon Aeropyrum pernix K1 into a specific carboxylesterase by making a single mutation.In order to fulfill the urgent requirement of thermostable lipolytic enzymes,in this paper we describe how the substrate preference of apAAP can be further changed from p-nitrophenyl caprylate(pNP-C8)to p-nitrophenyl laurate(pNP-C12)by protein and solvent engineering.After one round of directed evolution and subsequent saturation mutagenesis at selected residues in the active site,three variants with enhanced activity towards pNP-C12 were identified.Additionally,a combined mutant W474V/F488G/R526V/T560W was generated,which had the highest catalytic efficiency(kcat/Km)for pNP-C12,about 71-fold higher than the wild type.Its activity was further increased by solvent engineering,resulting in an activity enhancement of 280-fold compared with the wild type in the presence of 30%DMSO.The structural basis for the improved activity was studied by substrate docking and molecular dynamics simulation.It was revealed that W474V and F488G mutations caused a significant change in the geometry of the active center,which may facilitate binding and subsequent hydrolysis of bulky substrates.In conclusion,the combination of protein and solvent engineering may be an effective approach to improve the activities of promiscuous enzymes and could be used to create naturally rare hyperthermophilic enzymes. | Chang Liu Guangyu Yang Lie Wu Guohe Tian Zuoming Zhang Yan Feng | 2011 | Protein & Cell2011，2，6： | 0 |
| 6 | Comprehensive Evaluation Analysis of Airlines Frequent Flyer Value显示文摘 | Guohe Feng Xiaoxiao Zhang Xia Feng Yun Xue | 2014 | 电子商务学刊(中英文版)2014，3，1： | 0 |
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