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| 1 | In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene显示文摘We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2 by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells:MT-901/HER2 or MCA-205/ HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells. | JIAN MIN YANG MICHAEL S FRIEDMAN MARIANNE T HUBEN JENNIFER FULLER QIAO LI ALFRED E CHANG JAMES J MULE KEVIN T MCDONAGH | 2006 | Journal of Microbiology and Immunology2006，4，2： | 3 |
| 2 | 转染嵌合性T细胞受体基因小鼠T淋巴细胞的体外抗肿瘤作用显示文摘目的:研究小鼠T淋巴细胞在转染嵌合性T细胞受体基因后的体外抗肿瘤作用。方法:应用重组DNA技术,将HER2特异性嵌合性T-细胞受体构建入逆转录病毒载体;转入包装细胞后,收集病毒上清,转染小鼠T淋巴细胞,转基因后的小鼠T淋巴细胞分别与HER2阳性(SK-OV-3)或阴性(MCF-7)的肿瘤细胞系共培养,检测其细胞因子γ干扰素释放,51Cr释放法检测CTL评价其抗肿瘤效应。结果:所构建载体经酶切鉴定符合要求,乒乓法转染包装细胞系GP+E86,检测病毒滴度为1.2×106,Retronectin结合离心法转染经抗CD3/CD28单抗活化的小鼠T淋巴细胞,转染效率可达50%以上;转染嵌合性T细胞受体基因的T淋巴细胞与HER2阳性或阴性的肿瘤细胞系共培养后可检测到HER2特异性的细胞因子γ干扰素释放,51Cr释放法测CTL可见转染嵌合性T细胞受体基因T淋巴细胞对HER2阳性的肿瘤细胞具显著杀伤效应。结论:转染嵌合性T细胞受体基因的小鼠T淋巴细胞在体外可通过细胞因子释放和CTL效应发挥显著的抗肿瘤作用。 | 杨建民 Michael S Friedman 李峤 James J Mule Alfred E Chang Kevin T McDonagh | 2006 | 中国肿瘤生物治疗杂志2006，13，4： | 2 |
| 3 | Low-power repeaters driving RC and RLC interconnects with delay and bandwidth constraints 显示文摘 | Chen G Q Friedman E G | 2006 | IEEE Transactions on Very Large Scale Integration (VLSI) Systems2006，14，2： | 1 |
| 4 | Inhibition of Rat Testicular Nuclear Kinesins (krp2; KIFC5A) by Acrylamide as a Basis for Establishing a Genotoxicity Threshold 显示文摘 | Friedman MA Zeiger E Marroni DE | 2008 | Food Chem2008，56，15： | 1 |
| 5 | Propping and Tunneling显示文摘 | E Friedman S Johnson and T Mitton | 2003 | Journal of Comparative Economic2003，31，4： | 1 |
| 6 | Propping and tunneling显示文摘 | Friedman E Johnson S Mitton T | 2003 | Journal of Comparative Economics2003，31，4： | 1 |
| 7 | Reputation systems显示文摘 | Resnick P Zeckhauser R Friedman E | 2000 | Communications of the ACM2000，43，12： | 1 |
| 8 | Reputation systems 显示文摘 | Resnickand P Zeekhuaserand R Friedman E | 2000 | Communications of the ACM2000，43，12： | 1 |
| 9 | Efficacy and safety of low dose fluticasone propionate compared with montelukast for maintenance treatment of persistent asthma显示文摘 | MELTZER E O LOCKEY R F FRIEDMAN B F | 2002 | Mayo Clin Proc2002，77，： | 1 |
| 10 | Density dependence in kaonic atom显示文摘 | FRIEDMAN E GAL A BATTY C J | 1993 | Phys Lett B1993，308，12： | 1 |
| 11 | Ego-identity status in college women显示文摘 | Marcia J E Friedman M F | 1970 | Journal of Personality1970，38，： | 1 |
| 12 | Molecular regulation of hepatic fibrogene- sis显示文摘 | Olaso E Friedman SL | 1998 | J Hepatol1998，29，： | 1 |
| 13 | Mechanisms of hepatic fibrogenesis 显示文摘 | Lee U E Friedman S L | 2011 | Best Praet Res Clin Gastroenterol2011，25，2： | 1 |
| 14 | The importance of urinary protein excretion during conservative management of severe preeclampsia显示文摘 | Schiff E Friedman SA Kao L | 1996 | Am J Obstet Gynecol1996，175，： | 1 |
| 15 | Echocardio- graphic evidence for impaired myocardial performance in chil- dren with type I diabetes mellitus显示文摘 | Friedman N E Levitsky L L Edidin D V | 1982 | Am J Med1982，73，6： | 1 |
| 16 | Stimulated D1 dopamine receptors coupled to multiple Ga proteins in different brain regions显示文摘 | Jin L Wang HY Friedman E | 2001 | J Neurochem2001，78，： | 1 |
| 17 | Adenotonsillectomy imptobes neurocongnitibe function in children with obstructive sleep apnea syndrome显示文摘 | Friedman BC Hendeles- Amitai A Kozminsky E | 2003 | Sleep2003，26，： | 1 |
| 18 | Propping and Tunneling 显示文摘 | Friedman E S Johnson T Mittton | 2003 | Journal of Comparative Economics2003，，31： | 1 |
| 19 | Lowering of plasma LDL cholesterol in hamsters by the tomato glycoalkaloid tomatine显示文摘 | Friedman M Fitch T E Yokoyama W E | 2000 | Food Chem Toxicol2000，38，7： | 1 |
| 20 | Clinical pharmacology profile of vorinostat, a histone deacetylase inhibitor 显示文摘 | IWAMOTO M FRIEDMAN E J SANDHU P | 2013 | Cancer Chemother Pharmacol2013，72，3： | 1 |
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