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| 1 | Characterization of two distinct neuraminidases from avian-origin human-infecting H7N9 influenza viruses显示文摘一个鸟起源的 H7N9 流行性感冒病毒的流行病最近出现在中国,感染 45 死于的 134 个病人。这是怀有 N9 serotype neuraminidase (NA ) 的一个流行性感冒病毒被知道了感染人的第一次。H7N9 病毒是分叉的并且至少二不同 NA 和红血球凝聚素() 分别地,被发现了从临床孤立。这些病毒的原型是 A/Anhui/1/2013 和 A/Shanghai/1/2013。当 A/Shanghai/1/2013 NA 有能授与 NA 禁止者 oseltamivir 抵抗的 R294K 替换,从这二个病毒的 NA 是不同的。Oseltamivir 迄今为止由于它的力量和高 bioavailability 是最通常使用的反流行性感冒药。在这研究,我们证明 R294K 替换用基于蛋白质、基于病毒的试金与极端 oseltamivir 抵抗(超过 100 000 褶层) 导致 multidrug 抵抗。为禁止者抵抗决定分子的基础,我们从 A/Anhui/1/2013 N9 (包含 R294 ) 和 A/Shanghai/1/2013 N9 (包含 K294 ) 解决了 NA 的高分辨率的水晶结构。在为 oseltamivir 绑定的相反的 E276 符合构造的 R294K 替换结果,并且因而禁止者羧化物相互作用的损失,它损害所有古典 NA ligands/inhibitors 的绑定。而且,我们与更低的病毒的健康一起在减少的 NA 催化效率发现那 R294K 替换结果。这帮助解释 K294 为什么主要在 oseltamivir 处理并且不在主导的人感染的病毒的选择压力下面在 H7N9 感染的临床的情况中被发现了。这暗示 oseltamivir 能高效地仍然在 H7N9 感染的处理被使用。 | YanWu #x0002A YuhaiBi #x0002A Christopher JVavricka #x0002A XiaomanSun YanfangZhang FengGao MinZhao HaixiaXiao ChengfengQin JianhuaHe WenjunLiu JinghuaYan JianxunQi George FGao | 2013 | Cell Research2013，，12： | 15 |
| 2 | Propranolol and verapamil inhibit mRNA expression of RyR2 and SERCA in L-thyroxin-induced rat ventricular hypertrophy显示文摘AIM: To study the alteration in the mRNA level of cardiac ryanodine receptor 2 (RyR2) and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) in L-thyroxin-induced hypertrophy. METHODS: L-thyroxin (500 g/kg) daily was injected for 10 d. RT-PCR was used to determine mRNA expression. RESULTS: An increase in the relative amount of RyR2 (111 %) and SERCA mRNA (65 %) expression was observed in the hypertrophied rats (RyR2: 77±11; SERCA: 87±10, n=9) compared with the normal rats (RyR2: 36±10; SERCA: 53±10, n=9). Propranolol was effective to inhibit the increase in RyR2 (51±7) and SERCA (63±13) mRNA expression in hypertrophied rats, respectively. Verapamil also reduced RyR2 (62±5) and SERCA (75±8) mRNA expression. CONCLUSION: Both RyR2 and SERCA mRNA level in L-thyroxin-induced cardiac hypertrophy was over-expressed and propranolol or verapamil inhibited the alteration. | Xiao-dongWU De-zaiDAI Qiu-pinZHANG FengGAO | 2004 | Acta Pharmacologica Sinica2004，25，3： | 7 |
| 3 | Dispersion of ventricular mRNA of RyR2 and SERCA2 associated with arrhythmogenesis in rats显示文摘AIM: To investigate the effect of CPU86017 on the changes of mRNA abundance of different calcium handling system in infarcted heart. METHODS: Rats were subjected to left coronary ligation to induce myocardial infarc- tion (MI). The treatment with either propranolol (Pro) 5 mg/kg ip or CPU86017 1, 2, and 4 mg/kg ip was initiated on the next day of operation and continued for 20 d. Medication with isoproterenol (Isop) 3 mg/kg sc started on the d 17-21. Ventricular mRNA abundance of ryanodine receptor 2 (RyR2), sarcoplasmic reticulum Ca2+-ATPase (SERCA2), L-type Ca2+ channel, and Na+/Ca2+exchanger (NCX1) were measured. RESULTS: Arrhythmic scores (AS) in the Isop group was raised up to 5.27±1.75 (P<0.01) vs myocardial infarction group 2.25±2.04 and sham group 1.50±1.73. The AS was depressed by Pro (1.63±1.53, P<0.01 vs Isop), and CPU86017 2 and 4 mg/kg (3.00±1.24, and 1.70±1.85, P<0.01 vs Isop). The significant dispersion of depressed mRNA abundance of RyR2 and SERCA2 was associated with an increase in AS in Isop group, and it was much depressed in the left than the right ventricle. The dispersion and depression of mRNA were restored significantly by Pro and CPU86017, asso- ciated with suppression on AS. In Isop group, the mRNA abundance of L-type Ca2+ channel was not changed; and a moderate increase in the mRNA of NCX1 was seen, the changes were regressed by Pro and CPU86017. CONCLUSION: Isop-induced arrhythmogenesis in MI heart was correlated mainly with a dispersion of depressed mRNA abundance in ventricle likely due to the consequence of PKA over-phosphorylation. A suppression of arrhythmia by Pro and CPU86017 resulted from a regression of the dispersion and depression of RyR2 and SERCA2. | Hong-lanWANG De-zaiDAI FengGAO Yan-pinZHANG FengLU | 2004 | Acta Pharmacologica Sinica2004，25，6： | 6 |
| 4 | Repression of allo-cell transplant rejection through CⅡTA ribonuclease P^+hepatocyte显示文摘AIM: Allo-cell transplant rejection and autoimmune responses were associated with the presence of class Ⅱmajor histocompatibility complex (MHC Ⅱ) molecules on cells.This paper studied the effect of Ribonuclease P (RNase P)against CIITA, which was a major regulator of MHCII molecules, on repressing the expression of MHCII molecules on hepatocyte.METHODS: M1-RNA is the catalytic RNA subunit of RNase P from Escherichia coli. It were constructed that M1-RNA with guide sequences (GS) recognizing the 452, 3408 site of CIITA by PCR from pTK117 plasmid, then were cloned into the EcoRI/Bg/II or EcoR//SalIsite of vector psNAV (osNAV-M1-452-GS, psNAV-M1-3408-GS) respectively. The target mould plate (3176-3560) of CIITA was obtained from Raji cell by RT-PCR, and then inserted into the XhoI/EcoRIof pGEM-7zf(+) plasmid (pGEM-3176). These recombinant plasmids were screened out by sequence analysis. psNAV-M 1-452-GS, psNAV-M1-3408-GS and its target RNA pGEM-3176 were transcribed and then mixed up and incubated in vitro. It showed that M1-3408-GS could exclusively cleave target RNA that formed a base pair with the GS. Stable transfectants of hepatocyte cell line with psNAVl-M1-3408-GS were tested for expression of class Ⅱ MHC through FCM, for mRNA abundance of MHCII, Ii and CIITA by RTPCR., for the level of IL-2 mRNA on T cell by mixed lymphocyte reaction.RESULTS: When induced with recombinant human interferon-gamma (IFN-γ), the expression of HLA-DR, -DP,-DQ on psNAV-M1-3408-GS+ hepatocyte was reduced 83.27 %, 88.93 %, 58.82 % respectively, the mRNA contents of CIITA, HLA-DR, -DP, -DQ and Ii decreased significantly.While T cell expressed less IL-2 mRNA in the case of psNAV-M1-3408-GS+ hepatocyte.CONCLUSION: The Ribonuclease P against CIITA-M1-3408-GS could effectively induce antigen-specific tolerance through cleaving CIITA. These results provided insight into the future application of M1-3408-GS as a new nucleic acid drug against allo-transplantation rejection and autoimmune diseases. | RongGuo PingZou Hua-HuaFan FengGao Qing-XinShang Yi-LinCao Hua-ZhongLu | 2003 | World Journal of Gastroenterology2003，9，5： | 5 |
| 5 | Endoscopic survey of esophageal cancer in a high-risk area of China显示文摘AIM: To characterize the histological types of esophageal and cardiac mucosa by endoscopic survey of a population in a high-risk area of esophageal cancer of China. METHODS: A selected cohort of residents in Cixian County during December 2001 and May 2002 was surveyed by using Lugol's staining, followed by computer-based statistical analysis of the data with SPSS 10.0 software. RESULTS: Histologically, the detection rates of squamous epithelial acanthosis, squamous epithelial atrophy, and basal cell hyperplasia in the esophagus were 1.9% (38/2013),0.1% (3/2013) and 0.9% (18/2013) respectively, and those of mild, moderate, and severe esophagitis were 34.9% (703/2013), 1.6% (33/2013) and 0.2% (2/2013) respectively. Mild, moderate, and severe esophageal dysplasia were detected in 8.6% (172/2013), 7.8% (157/2013) and 2.6% (53/2013) respectively in the selected population, whereas in situ carcinoma, intramucosal carcinoma, invasive squamous carcinoma of the esophagus in 2.5% (50/2013),0.2% (4/2013) and 0.7% (14/2013) respectively. The detection rates of non-atrophic gastritis and atrophic gastritis of the cardia were 36.3% (730/2013) and 11.5% (232/2013) respectively, with mild and severe dysplasia of the cardia detected in 2.5% (51/2013) and 0.8% (17/2013), respectively, in this population; the rates of intramucosal adenocarcinoma and invasive adenocarcinoma of the cardia were 0.1% (3/2013) and 0.8% (17/2013) respectively. The detection rate of esophageal cancer at early stage was 79.4% (54/68). The survey rate (ratio of examined population to expected population) was 73.8% (2013/2725). CONCLUSION: Histologic types of the esophageal and cardiac mucosa were characterized by endoscopic survey in a high-risk population of esophageal cancer, which may help the early detection and treatment of esophageal and cardiac cancers and dysplasia, and reduce the mortality of such malignancies. | Xu-JingLu Zhi-FengChen Cui-LanGuo Shao-SenLi Wen-LongBai Guo-LiangJin Yu-XiaWang Fan-ShuMeng FengGao JunHou | 2004 | World Journal of Gastroenterology2004，10，20： | 3 |
| 6 | C-kit gene mutation in human gastrointestinal stromal tumors显示文摘AIM: TO investigate the significance of c-kit gene mutation in gastrointestinal stromal tumors (GIST).METHODS: Fifty two cases of GIST and 28 cases of other tumors were examined. DNA samples were extracted from paraffin sections and fresh blocks. Exons 11, 9 and 13 of the c-kit gene were amplified by PCR and sequenced.RESULTS: Mutations of exon 11 were found in 14 of 25 malignant GISTs (56%), mutations of exon 11 of the c-kit gene were revealed in 2 of 19 borderline GISTs (10.5%),and no mutation was found in benign tumors. The mutation rate showed significant difference (x^2=14.39, P<0.01) between malignant and benign GISTs. Most of mutations consisted of the in-frame deletion or replication from 3 to 48 bp in heterozygous and homozygous fashions, None of the mutations disrupted the downstream reading frame of the gene. Point mutations and frame deletions were most frequently observed at codons 550-560, but duplications were most concentrated at codons 570-585. No mutations of exons 9 and 13 were revealed in GISTs, Neither c-kit gene expression nor gene mutations were found in 3 leiomyomas, 8 leiomyosarcomas, 2 schwannomas, 2 malignant peripheral nerve sheath tumors, 2 intra-abdominal fibromatoses, 2 malignant fibrous histiocytomas and 9 adenocarcinomas.CONCLUSION: C-kit gene mutations occur preferentially in malignant GISTs and might be a clinically useful adjunct marker in the evaluation of GISTs and can help to differentiate GISTs from other mesenchymal tumors of gastrointestinal tract, such as smooth muscle tumors,schwannomas, etc. | Ying-YongHou Yun-ShanTan Meng-HongSun Yong-KunWei Jian-FangXu Shao-HuaLu Su-JieA-Ke-Su Yan-NanZhou FengGao Ai-HuaZheng Tai-MingZhang Wen-ZhongHou JianWang XiangDu Xiong-ZengZhu | 2004 | World Journal of Gastroenterology2004，10，9： | 3 |
| 7 | Expression of Single‐Chain Fv Gene Specific for γ‐Seminoprotein by RTS and Its Biological Activity Identification显示文摘 | YuedongHan YiHaun JinlanDeng FengGao BifengPan DaxiangCui | 2008 | Biotechnol Progress2008，，4： | 1 |
| 8 | Singular linaer space and its application显示文摘 | WANG KAISHUN GUO JUN LI FENGGAO | 2011 | Finite Fields App2011，17，： | 1 |
| 9 | Kinematic Analysis and Design of a Novel 6-degree of Freedom Parallel Robot显示文摘 | HUIDU FENGGAO YANGPAN | 2014 | Proceedings of the Institution of Mechanical Engineers Part C:Journal of Mechanical Engineering Science2014，14，： | 1 |
| 10 | Fabricate organic thermoelectric modules use modified PCBM and PEDOT:PSS materials显示文摘在这篇论文,我们制作了一器官热电(TE ) 设备与修改[6,6 ]-phenyl-C61-butyric 酸甲基酉旨(PCBM ) 并且 poly (3,4-ethylenedioxythiophene ) 聚苯乙烯 sulfonate (PEDOT : PSS ) ;设备处于空气状况显示出好稳定性。为 n 腿, PCBM 与氮蒽橘子底(3,6 二度(dimethylamino ) 氮蒽)(AOB ) 和 1,3-dimethyl-2,3-dihydro-1H-benzoimidazole (N-DMBI ) 被做。共同做的 PCBM 利用 AOB 和 N-DMBI 的 synergistic 效果,导致优秀电的电导率和分别地,在有掺杂物的房间温度到达 2 S/cm 和 -500 mV/K 的 Seebeck 系数价值 0.11 的臼齿的比率。使用的 P 类型腿修改了 PEDOT : PSS。基于修改 PCBM 和 PEDOT : PSS 材料,我们与 48 p 类型和 n 类型热电偶制作了一台 TE 模块设备并且测试了他们的产量电压,短电流,和功率。产量电压测量了 ~ 0.82 V,和产生力量在 453 K 热方面的温度与 75 K 温度坡度到达了几乎 945 mW。这些有希望的结果在设备优化为申请作为 TE 材料显示出修改 PEDOT 和 PCBM 的潜力。 | FengGAO Yuchun LIU Yan XIONG Ping WU Bin HU Ling XU | 2017 | Frontiers of Optoelectronics2017，10，2： | 1 |
| 11 | End-to-end self-assembly and colorimetric characterization of gold nanorods and nanospheres via oligonucleotide hybridization显示文摘 | Bifeng Pan Limei Ao FengGao Hongye Tian Rong He Daxiang Cui | 2005 | Nanotechnology2005，16，： | 1 |
| 12 | Network structure, distribution and the growth of Chinese international research collaboration 显示文摘 | Niu Fenggao Qiu Junping | 2014 | Scientometrics2014，98，2： | 1 |
| 13 | Comparative study of regulatory T cells expanded ex vivo from cord blood and adult peripheral blood显示文摘 | HuahuaFan JieYang JunHao YanaRen LiangChen GuipingLi RufengXie YimingYang FengGao MingyaoLiu | 2012 | Immunology2012，，2： | 1 |
| 14 | A Survey of Esophageal Cancer in Cixian County of Hebei Province显示文摘OBJECTIVE To characterize the histologic types of esophageal cardiac mucosa by endoscopic survey in a high-risk cancer area of China. METHODS An endoscopic survey with Lugol,s staining was carried out in Cixian County, Hebei Province from December 2001 to May 2002. The data were processed using computer SPSS 10.0 software. RESULTS The incidences of mild esophagitis, moderate esophagitis, and severe esophagitis were for 2013 cases, 34.9%(703), 1.6%(33) and 0.1% (2)respectively; those with mild dysplasia, moderate dysplasia, severe dysplasia of the esophagus were 8.6% (172), 7.8% (157) and 2.6% (53) respectively; those with carcinoma in situ, intramucosal carcinoma, invasive squamous carcinoma of the esophagus were 2.5%(50), 0.2% (4) and 0.7%(14) respectively. The histologic-detecting rates of non-atrophic gastritis, and atrophic gastritis of the cardia were 36.3%(730), 11.5% (232) respectively; those with mild dysplasia, severe dysplasia of the cardia were 2.5%(51), 0.8%(17) respectively; those with intramucosal adenocarcinoma, invasive adenocarcinoma of the cardia were 0.1% (3), 0.8%(17) respectively. The early-detection rate of esophageal cancer was 79.4%(54/68). The survey rate(examined population to covered population) was 73.8% (2013/2725). CONCLUSIONS Esophageal endoscopic screening with Lugol's solution staining has an advantage over esophageal balloon cytology, in that the histological diagnoses of esophageal cardiac diseases can be obtained, thus contributing to the prevention of subsequent disease. In using the staining method the detection rate of early esophageal cancer is higher than that revealed by balloon cytology. | JunHou ZhifengChen CuilanGuo ShaosenLi WenlongBai GuolimngJin YuxiaWang FanshuMeng FengGao XujingLu ZhifangWang GangKuang | 2004 | Chinese Journal of Clinical Oncology2004，1，1： | 1 |
| 15 | Association schemes based on attenuated spaces显示文摘 | Wang Kaishun Guo Jun Li Fenggao | 2010 | European Journal of Combinatorics2010，31，： | 1 |
| 16 | Toll‐Like Receptor and Heme Oxygenase‐1 Signaling in Hepatic Ischemia/Reperfusion Injury显示文摘 | Xiu‐DaShen BiboKe YuanZhai FengGao Ronald W.Busuttil GenhongCheng Jerzy W.Kupiec‐Weglinski | 2005 | American Journal of Transplantation2005，，8： | 1 |
| 17 | Functional rice centromeres are marked by a satellite repeat and a Centmmere-Specific retrotransposon显示文摘 | Zhukuan Cheng Fenggao Dang Tim Langdon | 2002 | Plant Cell2002，14，8： | 1 |
| 18 | Association Schemes Based on Attenuated Spaces 显示文摘 | WANG Kaishun GUO Jun LI Fenggao | 2010 | European Journal of Combinatories2010，31，： | 1 |
| 19 | Singular Linear Space and Its Application 显示文摘 | WANG Kaishun GUO Jun LI Fenggao | 2011 | Finite Fields and Their Applications2011，17，： | 1 |
| 20 | Room‐Temperature Synthesis in Acidic Media of Large‐Pore Three‐Dimensional Bicontinuous Mesoporous Silica with Ia3d Symmetry显示文摘 | XiaoyingLiu BozhiTian ChengzhongYu FengGao SonghaiXie BoTu RenchaoChe Lian‐MiaoPeng DongyuanZhao | 2002 | Angewandte Chemie International Edition2002，，20： | 1 |
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