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| 1 | Generation of Reactive Oxygen Species in Different Fractions of the Coelomocytes of Holothurian Eupentacta fraudatrix in Response to the Thermostable Toxin of Yersinia pseudotuberculosis in Vitro显示文摘Pure fraction (92%-95%) of phagocytes (FP) and a mixture of amoebocytes(62%) and morula cells (38 %) FPMC of the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota) were obtained by using ficoll verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at different concentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5 fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. | Dolmatova L. S. Eliseykina M. G. Timchenko N. F. Kovaleva A. L. Shitkova O. A | 2003 | Chinese Journal of Oceanology and Limnology2003，21，4： | 3 |
| 2 | Antioxidant enzymatic activity of coelomocytes of the Far East sea cucumber Eupentacta fraudatrix显示文摘 | Dolmatova L S Eliseikina M G Romashina V V | 2004 | J Evol Biochem Phys2004，40，： | 1 |
| 3 | Cardiomyocyte ATP release through pannexin 1 aids in early fibroblast activation显示文摘 | Dolmatova E Spagnol G Boassa D | 2012 | Am J Physiol Heart Circ Physiol2012，303，10： | 1 |
| 4 | Antioxidant Enzymatic Activity of Coelomocytes of the Far East Sea Cucumber Eupentacta fraudatrix显示文摘 | L. S. Dolmatova M. G. Eliseikina V. V. Romashina | 2004 | Journal of Evolutionary Biochemistry and Physiology2004，，2： | 1 |
| 5 | Structural and molecular mechanisms of gap junction remodeling in epicardial border zone m- yocytes following myocardial infarction 显示文摘 | Kieken F Mutsaers N Dolmatova E | 2009 | Circ Res2009，104，9： | 1 |
| 6 | Quantitative analysis of paper coatings using artificial neural networks 显示文摘 | Dolmatova L Ruckebusch C Dupuy N | 1997 | Chemometrics Andintelligent Laboratory Systems1997，36，： | 1 |
| 7 | Structural and molecular mechanisms of gap junction remodeling in epicardial border zone myoeytes following myocardial infarction显示文摘 | Kieken F Mutsaers N Dolmatova E | 2009 | Circ Res2009，104，9： | 1 |
| 8 | Inhibition ofc -Src tyrosine kinase prevents angiotensin ii -mediated connexin -43remodeling and sudden cardiac death 显示文摘 | Sovari AA Iravanian S Dolmatova E | 2011 | J Am Coll Cardiol2011，58，22： | 1 |
| 9 | Antioxidant enzymatic activity of coelomocytes of the far east sea cucumber显示文摘 | Dolmatova L S Eliseikina M G RomashinaV | 2004 | Journal of Evolutionnary Biochemistry and Physiology2004，40，2： | 1 |
| 10 | Inhibition of c-Src tyro- sine kinase prevents angiotensin Ⅱ-mediated connexin-43 remode- ling and sudden cardiac death 显示文摘 | Sovari AA Iravanian S Dolmatova E | 2011 | J Am Coil Cardiol2011，58，22： | 1 |
| 11 | Mitochondria Oxidative Stress, Connexin43 Remodeling, and Sudden Arrhythmic Death显示文摘 | Ali A. Sovari Cody A. Rutledge Euy-Myoung Jeong Elena Dolmatova Divya Arasu Hong Liu Nooshin Vahdani Lianzhi Gu Shadi Zandieh Lei Xiao Marcelo G. Bonini Heather S. Duffy Samuel C. Dudley | 2013 | Circulation: Arrhythmia and Electrophysiology2013，，： | 1 |
| 12 | Scheduled dosing of lornoxicam provides analgesia superior to that provided by on request dosing follow- ing craniotomy 显示文摘 | Dolmatova EV Imaev AA Lubnin AY | 2009 | Eur J Anaesthesiol2009，26，8： | 1 |
| 13 | Inhibition of c-Src Tyrosine Kinase Prevents Angiotensin II–Mediated Connexin-43 Remodeling and Sudden Cardiac Death显示文摘 | Ali A. Sovari Shahriar Iravanian Elena Dolmatova Zhe Jiao Hong Liu Shadi Zandieh Vibhash Kumar Kun Wang Kenneth E. Bernstein Marcelo G. Bonini Heather S. Duffy Samuel C. Dudley | 2011 | Journal of the American College of Cardiology2011，，： | 1 |
| 14 | Struetural and molecular mechanisms of gap junetion remodeling in epicardial border zone myoeytes following myocardial infaretion 显示文摘 | Kieken F Mutsaers N Dolmatova E | 2009 | Circ Res2009，104，9： | 1 |
| 15 | ‘Scheduled,dosing oflomoxicam provides analgesia superior to that provided by ‘ onrequest ’ dosing following craniotomy 显示文摘 | Dolmatova EV Imaev AA Lubnin AY | 2009 | Eur J Anaesthesiol2009，26，8： | 1 |
| 16 | Antioxidant Enzymatic Activity of Coelomocytes of the Far East Sea Cucumber Eupentacta fraudatrix显示文摘 | Dolmatova L Eliseikina M Romashina V | 2004 | Journal of Evolutionary Biochemistry and Physiology2004，40，2： | 1 |
| 17 | Management of postoperative analgesia in patients after eraniotomy 显示文摘 | IMAEV A A DOLMATOVA E V LUBNIN A | 2013 | Zh VoW Neirokhir Im N N Burdenko2013，77，3： | 1 |
| 18 | Antioxidant Enzymatic Activity of Coelomocytes of the Far East Sea Cucumber Eupentacta fraudatrix显示文摘 | L. S. Dolmatova M. G. Eliseikina V. V. Romashina | 2004 | Journal of Evolutionary Biochemistry and Physiology2004，，2： | 1 |
| 19 | Inhibition of c-Src tyro- sine kinase prevents angiotensin II-mediated connexin-43 remodeling and sudden cardiac death显示文摘 | Sovari AA Iravanian S Dolmatova E | 2011 | J Am Coll Cardiol2011，58，22： | 1 |
| 20 | Management of postoperativeanalgesia in patients after craniotomy显示文摘 | Imaev AA Dolmatova EV Lubnin AIu | 2013 | Zh Vopr Neirokhir Im N N Bur-denko2013，77，3： | 1 |
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