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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Gastrin,somatostatin,and experimental disturbance of the gastrointestinal tract in rats显示文摘INTRODUCTIONThe field of gastrointestinal hormones has expanded at a dizzying rate[1-4].Gastrointestinal hormones as regulatory peptides that appear to be major components of bodily integration and have important regulatory actions on physioligical function of the gastrointestinal tract .The successful isolation of some gastrointestinal hormones and the development of sensitive methods for their detection have led to the unexpected finding that they also exist in the brain . | Yong Li Yao~1 Bo Xu~2 Wan Dai Zhang~1 Yu Gang Song~1 ~1Institute of Gastrointestinal Diseases,Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2Department of Orthopedics Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaYong Li Yao graduated from First Military Mcdical University with a master degree in 1998.She now works in the Institute of Gastrointestinal Diseases,Nanfang Hospital as a doctoral candidate majoring gastrointestinal diseases and gastrointestinal hormones,she has published 8 papers. | 2001 | World Journal of Gastroenterology2001，7，3： | 12 |
| 3 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 4 | Use of phosphite salts in laboratory and semicommercial tests to control citrus postharvest decay显示文摘 | Cerioni L Rapisarda V A Doctor J | 2013 | Plant Disease2013，97，2： | 1 |
| 5 | Theeffectiveness of leflunomide as a co-therapy of tumour necrosis factorinhibitors in rheumatoid arthritis: a population-based study 显示文摘 | Finekh A Dehler S Gabay C on behalf of the SCQM doctors | 2009 | AnnRheum Dis2009，，： | 1 |
| 6 | Effect of processing and storage on red blood cell function in vivo显示文摘 | Doctor A SpineUa P | 2012 | Semin Perinatol2012，36，4： | 1 |
| 7 | Hydrogen production by direct contact pyrolysis of natural gas显示文摘 | SERBAN M LEWIS M A MARSHALL C L DOCTOR R D | 2003 | Energy Fuels2003，17，3： | 1 |
| 8 | Effects of nitroglycerin on erythrocyte rheology and oxygen unloading: Novel role of s-nitrosohemoglobin in relieving myocardial ischemia 显示文摘 | Bin JP Doctor A Lindner J | 2006 | Circulation2006，113，21： | 1 |
| 9 | Isolation and aticoagulant pro-pertiec of polysaccharides of typha angustata and dae monorops species显示文摘 | Gibbs A Greec E Doctor V M | 1983 | Thromb Res1983，32，2： | 1 |
| 10 | Evolution of adverse changes in stored RBCs 显示文摘 | Bennett-Guerrero E Veldman TH Doctor A | 2007 | Proc Natl Acad Sci USA2007，104，17： | 1 |
| 11 | S-Nitrosothiols signalhypoxia-mimetic vascular pathology显示文摘 | Palmer LA Doctor A’Chhabra P | 2007 | J Clin Invest2007，117，9： | 1 |
| 12 | Extrapulmonary effects of inhaled nitric oxide :role of reversible S-nitrosylation of erythrocytic hemoglobin 显示文摘 | Mcmahon TJ Doctor A | 2006 | Proc AmThorac Soc2006，3，2： | 1 |
| 13 | Extrapulmonary effects of inhaled nitric oxide role of reversible S - nitrosylation of erythrocytic hemoglobin 显示文摘 | McMahon TJ Doctor A | 2006 | Proc Am Thorac Soc2006，3，2： | 1 |
| 14 | Alveolar macrophage activa- tion is a key initiation signal for acute lung isehemla-reperfusion inju- ry显示文摘 | Zhao M Femandez LG Doctor A | 2006 | Am J Physiol Lung Cell Mol Physiol2006，291，5： | 1 |
| 15 | Does the storage duration of blood products affect outcomes in critically ill patients 显示文摘 | Spinella PC Doctor A Blumberg N | 2011 | Transfusion2011，51，8： | 1 |
| 16 | Concentration-dependent effects of endogenous S-nitrosoglutathione on gene regulation by specificity protein Sp3 and Spl显示文摘 | Zaman K Palmer LA Doctor a | 2004 | Biochem J2004，380，1： | 1 |
| 17 | Isolation and atieoagulant propertiec of polysaccharides of Typha angustata and dae monorops species显示文摘 | Gibbs A Greec E Doctor V M | 1983 | Thromb Res1983，32，2： | 1 |
| 18 | Evolution of adverse changes in stored RBCs 显示文摘 | Bennett-Guerrero E Veldman TH Doctor A | 2007 | Proc Natl Acad Sci U S A2007，104，17： | 1 |
| 19 | Effect of oversulfation on thechemicalandbiolo~calproperties of fucoidan显示文摘 | Qiu x D Amarasekara A Vasant Doctor | 2006 | Carbohyd Polym2006，63，： | 1 |
| 20 | Evolution of adverse changes in stored RBCs显示文摘 | Bennett-Guerrero E Veldman TH Doctor A | 2007 | Proc Natl Acad Sci U S A2007，104，17： | 1 |
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