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您的检索式：作者名="Dickson M.D.Siu"
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| 1 | Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array显示文摘Parallelized fluorescence imaging has been a long-standing pursuit that can address the unmet need for a comprehensive three-dimensional(3D)visualization of dynamical biological processes with minimal photodamage.However,the available approaches are limited to incomplete parallelization in only two dimensions or sparse sampling in three dimensions.We hereby develop a novel fluorescence imaging approach,called coded light-sheet array microscopy(CLAM),which allows complete parallelized 3D imaging without mechanical scanning.Harnessing the concept of an“infinity mirror”,CLAM generates a light-sheet array with controllable sheet density and degree of coherence.Thus,CLAM circumvents the common complications of multiple coherent light-sheet generation in terms of dedicated wavefront engineering and mechanical dithering/scanning.Moreover,the encoding of multiplexed optical sections in CLAM allows the synchronous capture of all sectioned images within the imaged volume.We demonstrate the utility of CLAM in different imaging scenarios,including a light-scattering medium,an optically cleared tissue,and microparticles in fluidic flow.CLAM can maximize the signal-to-noise ratio and the spatial duty cycle,and also provides a further reduction in photobleaching compared to the major scanning-based 3D imaging systems.The flexible implementation of CLAM regarding both hardware and software ensures compatibility with any light-sheet imaging modality and could thus be instrumental in a multitude of areas in biological research. | Yu-Xuan Ren Jianglai Wu Queenie T.K.Lai Hei Ming Lai Dickson M.D.Siu Wutian Wu Kenneth K.Y.Wong Kevin K.Tsia | 2020 | Light(Science & Applications)2020，9，1： | 2 |
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