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| 1 | Desferrioxamine in warm reperfusion media decreases liver injury aggravated by cold storage显示文摘AIM:To evaluate whether desferrioxamine decreases ischemia and perfusion injury aggravated by cold storage(CS)in a rat liver perfusion model. METHODS:Isolated rat livers were kept in CS in University of Wisconsin Solution for 20 h at 4℃,then exposed to 25 min of warm ischemia(WI)at 37℃ followed by 2 h of warm perfusion(WP)at 37℃with oxygenated(95%oxygen and 5%carbon dioxide) Krebs-Henseleit buffer.Desferrioxamine(DFO),an iron chelator,was added at different stages of storage,ischemia and perfusion:in CS only,in WI only,in WP only, in WI and perfusion,or in all stages.Effluent samples were collected after CS and after WI.Perfusate samples and bile were collected every 30 min(0,0.5,1,1.5 and 2 h)during liver perfusion.Cellular injury was assessed by the determination of lactate dehydrogenase(LDH) and aspartate aminotransferase(AST)in the effluent and perfusate samples.Total iron was analysed in the perfusate samples.After WP,the liver was collected for the determination of liver swelling(wet to dry ratio) and liver morphological examination(hematoxylin and eosin staining). RESULTS:Increased CS time caused increased liver dysfunction during WP.After 2 h of WP,liver injury was indicated by increased release of AST(0.5 h CS:9.4± 2.2 U/g liver vs 20 h CS:45.9±10.8 U/g liver,P<0.05) and LDH(0.5 h CS:59±14 U/g liver vs 20 h CS:297 ±71 U/g liver,P<0.05).There was an associated increase in iron release into the perfusate(0.5 h CS:0.11 ±0.03μmoL/g liver vs 20 h CS:0.58±0.10μmoL/g liver,P<0.05)and reduction in bile flow(0.5 h CS: 194±12μL/g vs 20 h CS:71±8μL/g liver,P<0.05). When DFO was added during WI and WP following 20 h of CS,release of iron into the perfusate was de- creased(DFO absent 0.58±0.10μmoL/g liver vs DFO present 0.31±0.06μmoL/g liver,P<0.05),and liver function substantially improved with decreased release of AST(DFO absent 45.9±10.8 U/g liver vs DFO present 8.1±0.9 U/g liver,P<0.05)and LDH(DFO absent 297±71 U/g liver vs DFO present 56±7 U/g liver,P<0.05),and increased bile flow(DFO absent 71±8μL/g liver vs DFO present 237±36μL/g liver, P<0.05).DFO was also shown to improve liver morphology after WP.Cellular injury(the release of LDH and AST)was significantly reduced with the addition of DFO in CS medium but to a lesser extent compared to the addition of DFO in WP or WI and perfusion.There was no effect on liver swelling or bile flow when DFO was only added to the CS medium. CONCLUSION:DFO added during WI and perfusion decreased liver perfusion injury aggravated by extended CS. | Peter G Arthur Xian-Wa Niu Wen-Hua Huang Bastiaan DeBoer Ching Tat Lai Enrico Rossi John Joseph Gary P Jeffrey | 2013 | World Journal of Gastroenterology2013，19，5： | 2 |
| 2 | A Fast Cross-Entropy Method for Estimating Buffer Overflows in Queueing显示文摘 | DeBoer P T Kroese D P Rubinstein R Y | 2004 | Networks Management Science2004，50，： | 1 |
| 3 | Thoracic epidural anesthesia reduces myocardial infarct size after coronary artery occlusion in dogs显示文摘 | Davis R F DeBoer L W Maroko P R | 1986 | Anesth Analg1986，65，7： | 1 |
| 4 | Cardiovascular events in the postanesthesia care unit:contribution of risk factors显示文摘 | Rose D Cohen M Deboer P | 1996 | Anesthesiology1996，84，： | 1 |
| 5 | Dacite genesis via both slab melting and differentiation:Petrogenesis of La Yeguada volcanic complex,Panama显示文摘 | Defant M J Richerson P M DeBoer J Z | 1991 | J Petrol1991，32，6： | 1 |
| 6 | Low-stress PECVD SiC thin films for IC-compatible microstructures显示文摘 | Sarro P M DeBoer C R | 1998 | Sensors and Actuators1998，67，： | 1 |
| 7 | Latency of plasmamembrane H+-ATPase in vesicles isolated by aqueous phase pati-tioning显示文摘 | SandstromR P Deboer A H Lomax T L | 1987 | Plant Physiol1987，85，3： | 1 |
| 8 | Exogenous administration of thiosulfate,a donor of hydrogen sulfide,attenuates Angiotensin II-induced hypertensive heart disease in rats显示文摘 | SNIJDER P M FREBAY A S DEBOER R A | 2014 | Br J Pharmacol2014，，： | 1 |
| 9 | Extracellular replication of listeria monocytogenes in the murine gall bladder 显示文摘 | Hardy J Francis K P DeBoer M | 2004 | Science2004，303，： | 1 |
| 10 | Geldanamycin, a new antibiotic显示文摘 | Deboer C Meulmna P A Wnuk R J | 1979 | JAntibiot1979，32，4： | 1 |
| 11 | Physiological release of striatal acetylcholine in vivo:modulation by D1 and D2 dopamine receptor subtypes显示文摘 | DeBoer P Abercrombie ED | 1996 | J Pharmacol Exp Ther1996，277，2： | 1 |
| 12 | Cardiovascular events in the postanesthesia care unit:contribution of risk factors显示文摘 | Rose D Cohen M Deboer P | 1996 | Anesthesiology1996，84，： | 1 |
| 13 | Biodiversity hotspots and conservation priorities in the Campo-Ma' an rain forests, Cameroon 显示文摘 | Tchouto M G P Yemefack M Deboer W F | 2006 | Biodiversity and Conservation2006，15，： | 1 |
| 14 | Exchange nailing for femoral shaft aseptic non-union 显示文摘 | Furlong AJ Giannoudis PV DeBoer P | 1999 | Injury1999，30，4： | 1 |
| 15 | The postope: rative interview:assessing risk factors for nausea and vomi ting 显示文摘 | Cohen M M Duncan P G DeBoer D P | 1994 | Anesth Analg1994，78，1： | 1 |
| 16 | Pyruvate enhances recovery of rat heart after ischemia and reperfusion by preventing free radical generation显示文摘 | DeBoer JC Bekx P Han L | 1993 | Am J Physiol1993，34，： | 1 |
| 17 | Renal dysfunction is associatedwith shorter telomere length in heart failure显示文摘 | Wong LS van der Harst P Deboer RA | 2009 | Clin Res Cardiol2009，98，： | 1 |
| 18 | Advances in the gas chromatographic determination of persistent organic pollutants in the aquatic environment 显示文摘 | VanLeeuwen S P J deBoer J | 2008 | J Chromatogr A2008，1186，12： | 1 |
| 19 | Specialized ribosomes:Highly specific translation in vivo of a single targeted mRNA species显示文摘 | Verbeet M P Deboer H A | 1995 | Gene1995，156，2： | 1 |
| 20 | Exchange nailing for femoral shaft aseptic non-union显示文摘 | Furlong AJ Giannoudis PV DeBoer P | 1999 | Injury1999，4，： | 1 |
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