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| 1 | Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds. | Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes | 2017 | World Journal of Gastroenterology2017，23，2： | 6 |
| 2 | Tenofovir Is Effective Alone or With Emtricitabine in Adefovir-Treated Patients With Chronic-Hepatitis B Virus Infection显示文摘 | Thomas Berg Patrick Marcellin Fabien Zoulim Bernd Moller Huy Trinh Sing Chan Emilio Suarez Fabien Lavocat Andrea Snow–Lampart David Frederick Jeff Sorbel Katyna Borroto–Esoda David Oldach Franck Rousseau | 2010 | Gastroenterology2010，，4： | 2 |
| 3 | Carbon dioxide reaction processes in a model brine aquifer at 200 ℃ and 200 bars: implications for geologic sequestration of carbon 显示文摘 | John P Kaszuba David R Janecky Marjorie G Snow | 2003 | Applied Geochemistry2003，18，： | 1 |
| 4 | Occurrence of antimicrobials and antimicrobial resistance genes in beef cattle storage ponds and swine treatment lagoons显示文摘 | Yuping Zhang Chiqian Zhang David B. Parker Daniel D. Snow Zhi Zhou Xu Li | 2013 | Science of the Total Environment2013，，： | 1 |
| 5 | Frame Alignment Processes, Micro-mobilization and Movement Participation 显示文摘 | David Snow E Rochford S Worden and R Benford | 1986 | American Sociological Review1986，51，4： | 1 |
| 6 | Characterization of human sperm antigens and antispern antibodies in infertile patients显示文摘 | Snow Karen David Ball G | 1992 | Fertil Steril1992，58，： | 1 |
| 7 | Focused Inductive Theorem Proving 显示文摘 | David Baelde Dale Miller Zachary Snow | 2010 | Lecture Notes in Computer Science2010，6173，： | 1 |
| 8 | Caregiver Outcomes of Partners in Dementia Care: Effect of a Care Coordination Program for Veterans with Dementia and Their Family Members and Friends显示文摘 | David M. Bass Katherine S. Judge A. Lynn Snow Nancy L. Wilson Robert Morgan Wendy J. Looman Catherine A. McCarthy Katie Maslow Jennifer A. Moye Ronda Randazzo Maurilio Garcia‐Maldonado Richard Elbein Germaine Odenheimer Mark E. Kunik | 2013 | J Am Geriatr Soc2013，，8： | 1 |
| 9 | Health-related quality of life in patients with chronic hepatitis C and advanced fibrosis显示文摘 | Herbert L. Bonkovsky Kristin K. Snow Peter F. Malet Carla Back-Madruga Robert J. Fontana Richard K. Sterling Clark C. Kulig Adrian M. Di Bisceglie Timothy R. Morgan Jules L. Dienstag Marc G. Ghany David R. Gretch | 2006 | Journal of Hepatology2006，，3： | 1 |
| 10 | Reagents for the preparation of chromophori-cally labeled polyethylene glycol-protein conjugates显示文摘 | David L Ladd RobetA Snow | 1993 | Analytical Bioche-mistry1993，210，： | 1 |
| 11 | Social Networks and Social Movements: A Micro structural Approach to Differential Recruitment 显示文摘 | Snow David A Louis A Zurcher Sheldon Ekland-Olson | 1980 | American Sociology Review1980，，45： | 1 |
| 12 | Social Networks and Social Movements: A Microstructural Apprlach to Differential Recruitment显示文摘 | Snow David A Louis A Zurcher and Sheldon Ekland-Olson | 1980 | American Sociology Reviewl1980，45，： | 1 |
| 13 | Sensitive and simplified analysis of natural and synthetic steroids in water and solids using on-line solid-phase extraction and microwave-assisted solvent extraction coupled to liquid chromatography tandem mass spectrometry atmospheric pressure photoioni显示文摘 | Daniel D. Snow Teyona Damon-Powell Sathaporn Onanong David A. Cassada | 2013 | Analytical and Bioanalytical Chemistry2013，，5： | 1 |
| 14 | FRAMING PROCESSES AND SOCIAL MOVEMENTS: An Overview and Assessment显示文摘 | Robert D. Benford David A. Snow | 2000 | Annual Review of Sociology2000，，： | 1 |
| 15 | Strategic Entrepreneurship,Collaborative Innovation,and Wealth Creation显示文摘 | KETCHEN JR DAVID J IRELAND R D SNOW C C | 2007 | Entrepreneurship Theory and Practice2007，1，34： | 1 |
| 16 | 伦敦塔码头餐厅显示文摘餐厅位于伦敦塔码头至泰晤士河沿岸的伦敦塔桥和伦敦塔的交叉处。伦敦塔被联合国教科文组织列为世界文化遗产,游客在这里能够将大伦敦议会大厦和贝尔法斯特号巡洋舰等景观尽收眼底。英国政府曾委任“历史皇家宫殿组织“对该地区进行新的发展规划。作为一家独立的慈善机构,该组织目前管理着伦敦塔、汉普顿宫、国宴厅、肯辛顿宫以及裘园等众多景观建筑。 | Tony Fretton Jim McKinney David Owen Chris Snow Daniel Buckley Guy Derwent Yuichi Hashimura Peter Cook 谢作标 | 2012 | 现代装饰2012，，12： | 0 |
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