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您的检索式：作者名="Dangwei SHI"
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| 1 | Investigation of the thermal stability of Mn ferrite particles synthesized by a modified co-precipitation method显示文摘Manganese ferrite particles with spinel structure were synthesized by a modified co-precipitation method,and then the thermal stability on their structural and magnetic properties was investigated by thermal annealing at different gas ambients.Experimental results showed that decomposition of metal oxides occurred when Mn ferrite particles with spinel structure were annealed under air ambient,while samples annealed in vacuum or argon remained in the spinel structural form.The highest saturation magnetization value of 58 emu/g was obtained when the sample was annealed at 400℃ in argon.The result showed that the thermal stability of MnFe2O4 was better under Ar ambient conditions or in vacuum. | DONG ChunHui WANG GaoXue SHI Lei GUO DangWei JIANG ChangJun XUE DeSheng | 2013 | Science China Chemistry2013，56，3： | 3 |
| 2 | Phylogenetic relationships and divergence times of the family Araucariaceae based on the DNA sequences of eight genes显示文摘Araucariaceae is one of the most primitive families of the living conifers, and its phylogenetic rela-tionships and divergence times are critically important issues. The DNA sequences of 8 genes, i.e., nuclear ribosomal 18S and 26S rRNA, chloroplast 16S rRNA, rbcL, matK and rps4, and mitochondrial coxI and atp1, obtained from this study and GenBank were used for constructing the molecular phylogenetic trees of Araucariaceae, indicating that the phylogenetic relationships among the three genera of this family should be ((Wollemia, Agathis), Araucaria). On the basis of the fossil calibrations of Wol-lemia and the two tribes Araucaria and Eutacta of the genus Araucaria, the divergence time of Arauca-riaceae was estimated to be (308 ± 53) million years ago, that is, the origin of the family was in the Late Carboniferous rather than Triassic as a traditional view. With the same gene combination, the diver-gence times of the genera Araucaria and Agathis were (246 ± 47) and (61 ± 15) Ma, respectively. Statistical analyses on the phylogenetic trees generated by using different genes and comparisons of the divergence times estimated by using those genes suggested that the chloroplast matK and rps4 genes are most suitable for investigating the phylogenetic relationships and divergence times of the family Araucariaceae. | LIU Nian ZHU Yong WEI ZongXian CHEN Jie WANG QingBiao JIAN ShuGuang ZHOU DangWei SHI Jing YANG Yong ZHONG Yang | 2009 | Chinese Science Bulletin2009，54，15： | 1 |
| 3 | Cloning and Bioinformatics Analysis of Resveratrol Synthase Gene from Vitis vinifera显示文摘[Objectives] To obtain a resveratrol synthase gene of Vitis vinifera and make bioinformatics analysis. [Methods] Taking total RNA of V. vinifera as the template,by RT-PCR method,a complete c DNA sequence of resveratrol synthase gene was amplified from V. vinifera,and the resveratrol synthase gene was named as RS. The nucleic acid and protein sequences were analyzed using bioinformatics software.[Results]This sequence was 1179 bp in length,the similarity with reported resveratrol synthase gene reached 94%-99%,and the similarity with amino acid sequence reached 96%-99%; the RS gene encoded 392 amino acids,and amino acid sequence contained complete characteristic sequence GVLFGPGLT and active center sequence GCYAGGTVLR of stilbene synthase family; the predicted molecular weight was42. 78 k Da,the theoretical isoelectric point was 6. 57,the instability parameter was 35. 92,and it belonged to stable protein in the classification; the secondary structure was mainly α-helix,random coil and β-folding,α-helix content was 44. 13%,the random coil content was26. 53%,and β-folding content was 17. 66%. [Conclusions] The isolated RS gene is a resveratrol synthase gene from V. vinifera. This experiment is expected to lay a certain foundation for biosynthesis of resveratrol by the genetic engineering method. | Yi ZHAI Qinsong LIU Yongkun WU Yun MA Nianqiang ZHANG Dangwei SHI Qingsheng QI | 2018 | Medicinal Plant2018，9，2： | 0 |
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