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| 1 | Component analysis of Chinese medicine and advances in fuming-washing therapy for knee osteoarthritis via unsupervised data mining methods显示文摘OBJECTIVE: To analyze the component law of Chinese medicines in fuming-washing therapy for knee osteoarthritis(KOA), and develop new fuming-washing prescriptions for KOA through unsupervised data mining methods.METHODS: Chinese medicine recipes for fuming-washing therapy for KOA were collected and recorded in a database. The correlation coefficient among herbs, core combinations of herbs, andnew prescriptions were analyzed using modified mutual information, complex system entropy cluster, and unsupervised hierarchical clustering, respectively.RESULTS: Based on analysis of 345 Chinese medicine recipes for fuming-washing therapy, 68 herbs occurred frequently, 33 herb pairs occurred frequently, and 12 core combinations were found.Five new fuming-washing recipes for KOA were developed.CONCLUSION: Chinese medicines for fuming-washing therapy of KOA mainly consist of wind-dampness-dispelling and cold-dispersing herbs, blood-activating and stasis-resolving herbs,and wind-dampness-dispelling and heat-clearing herbs. The treatment of fuming-washing therapy for KOA also includes dispelling wind-dampness and dispersing cold, activating blood and resolving stasis, and dispelling wind-dampness and clearing heat. Zhenzhutougucao(Herba Speranskiae Tuberculatae), Honghua(Flos Carthami), Niuxi(Radix Achyranthis Bidentatae), Shenjincao(Herba Lycopodii Japonici), Weilingxian(Radix et Rhizoma Clematidis Chinensis), Chuanwu(Radix Aconiti), Haitongpi(Cortex Erythrinae Variegatae), Ruxiang(Olibanum),Danggui(Radix Angelicae Sinensis), Caowu(Radix Aconiti Kusnezoffii), Moyao(Myrrha), and Aiye(Folium Artemisiae Argyi) are the main herbs used in the fuming-washing treatment for KOA. | Jun Liu Jianke Pan Yanping Wang Dingkun Lin Dan Shen Hongjun Yang Xiang Li Minghui Luo Xuewei Cao | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 32 |
| 2 | Clinical significance of K-ras and BRAF mutations in Chinese colorectal cancer patients显示文摘AIM:To identify and assess mutations in the K-ras and BRAF genes in a cohort of Chinese patients with colorectal cancer (CRC) for their association with various clinicopathological parameters and prognosis.METHODS:Genomic DNA was isolated from frozen tissues.Pyrosequencing analysis was conducted to detect mutations in the K-ras (codons 12,13,and 61) and BRAF genes (codon 600).Statistical analysis was carried out using SPSS-15.0 software.RESULTS:Among the 118 colorectal cancer patients,we detected 41 (34.7%) mutations in the K-ras gene.Mutation frequencies at codon 12 and codon 13 were 23.7% (28/118) and 10.2% (12/118),respectively.Only one patient harbored a point mutation at codon 61 (0.8%,1/118).Gender was the only factor that showed an obvious relationship with K-ras gene mutation (female 44.7% vs male 28.2%,P=0.037).Other clinicopathological features,such as age,location of the tumor,tumor differentiation,Tumor,Node and Metastases classification,and the Union for International Cancer Control staging,showed no positive relationship with K-ras gene mutations.No significant correlation was observed between the presence of K-ras mutations (codons 12,13,and 61) and the survival of the patients.BRAF mutations were rare,and only two patients (1.7%) harbored a detectable mutation at codon 600.CONCLUSION:K-ras gene mutation is a common event in our 118 Chinese CRC patients,with an obvious relationship with gender.However,it seems not to be an independent prognostic factor in CRC patients.The BRAF gene is rarely mutated in Chinese CRC patients. | Hong Shen Ying Yuan Han-Guang Hu Xiao-Xian Ye Mo- Dan Li Xian Zhong Wei-Jia Fang Shu Zheng | 2011 | World Journal of Gastroenterology2011，17，6： | 29 |
| 3 | Nosocomial bloodstream infection in patients caused by Staphylococcus aureus: drug susceptibility, outcome, and risk factors for hospital mortality显示文摘背景以前的研究在获得医院的血液感染( BSI )的死亡上关于 methicillin 抵抗的临床的影响有不同观点有这研究客观的 .The 是在一所军事医院和分析里与 S.aureus 调查获得医院的 BSI 的死亡的葡萄球菌 aureus ( S.aureus )的病人为医院的风险因素 mortality.Methods A 回顾的队学习在进入最大的军事第三级的教学 hos | Chen Rong Yan Zhong-qiang Feng Dan Luo Yan-ping Wang Lei-li Shen Ding-xia | 2012 | Chinese Medical Journal2012，，2： | 22 |
| 4 | Ratios of T-helper 2 Cells to T-helper 1 Cells and Cytokine Levels in Patients with Hepatitis B显示文摘 | Ming-Hui Li Dan Zhang Lu Zhang Xiao-Jing Qu Yao Lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping Hu Hong-Xiao Hao Wen-Hao Hua Shu-Jing Song Gang Wan Shun-Ai Liu Yao Xie | 2017 | Chinese Medical Journal2017，，15： | 21 |
| 5 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 6 | Association of Cytokines with Alanine Aminotransferase, Hepatitis B Virus Surface Antigen and Hepatitis B Envelope Antigen Levels in Chronic Hepatitis B显示文摘 | Ming-Hui Li Yao Lu Lu Zhang Xing-Yue Wang Chong-Ping Ran Hong-Xiao Hao Dan Zhang Xiao-Jing Qu Ge Shen Shu-Ling Wu Wei-Hua Cao Tian-Lin Qi Ru-Yu Liu Lei-Ping Hu Min Chang Wen-Hao Hua Shun-Ai Liu Gang Wan Yao Xie | 2018 | Chinese Medical Journal2018，，15： | 17 |
| 7 | Plasmacytoid Dendritic Cell Function and Cytokine Network Profiles in Patients with Acute or Chronic Hepatitis B Virus Infection显示文摘 | Ming-Hui Li Lu Zhang Dan Zhang Wei-Hua Cao Tian-Lin Qi Hong-Xiao Hao Xing-Yue Wang Chong-Ping Ran Xiao-Jing Qu Shun-Ai Liu Yao Lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping Hu Wen-Hao Hua Gang Wan Jun Cheng Yao Xie | 2018 | Chinese Medical Journal2018，，1： | 16 |
| 8 | Upregulation of CD81 in trophoblasts induces an imbalance of Treg/Th17 cells by promoting IL-6 expression in preeclampsia显示文摘The disturbance of maternal immune tolerance to a semiallogeneic fetus is recognized as one of the key pathologies of preeclampsia(PE),in which an imbalance between the inflammation-limiting regulatory T cells(Tregs)and the inflammationmediating Th17 cells plays an essential role.Previously,we reported that the abnormal upregulation of tetraspannin CD81 in trophoblast cells(fetal component)participated in the pathogenesis of PE.However,as one of the potential immune regulatory molecules,whether CD81 induces PE by interfering with the balance of the maternal immune system has not yet been clarified.Thus,we investigated the relationship between the upregulation of CD81 in trophoblast cells and the imbalance of Treg and Th17 cells in mothers.Here,we demonstrated that upregulation of CD81 in trophoblast cells was accompanied by a decrease in Treg cells and an increase in Th17 cells in both the basal plate(placental maternal side)and peripheral blood of patients with PE.In vitro culture of naïve T cells with medium from the CD81-overexpressing trophoblast cell line HTR-8 resulted in enhanced differentiation of T cells into Th17 cells and decreased the formation of Tregs,which was dependent on the paracrine signaling of IL-6 in trophocytes,induced by CD81.In a CD81-induced PE rat model,we found a significant shift of T cell differentiation towards Th17 cells,and administration of IL-6 antibody mitigated the PE phenotype and the imbalance of the Treg/Th17 cells.These results define a vital regulatory cascade involving trophocyte-derived CD81,IL-6,and maternal Treg/Th17 cells in the pathogenesis of PE and suggests new therapeutic approaches based on CD81 and IL-6 downregulation to prevent human PE. | Hailin Ding Yimin Dai Yi Lei Zhiyin Wang Dan Liu Ruotian Li Li Shen Ning Gu Mingming Zheng Xiangyu Zhu Guangfeng Zhao Yali Hu | 2019 | Cellular & Molecular Immunology2019，16，3： | 15 |
| 9 | PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by regulating synaptic transmission in cerebellum显示文摘在充满脯氨酸的 transmembrane 蛋白质的变化(PRRT2 ) 2 与发作性的 kinesigenic 运动障碍(PKD ) 和几另外的发作性的神经病学的疾病被联系,但是 PRRT2 功能和病原的机制仍然保持大部分阴暗。这里,我们证明 PRRT2 是与 SNARE 建筑群和 downregulates 的部件交往的 presynaptic 蛋白质它的形成。Loss-of-function 变异的老鼠显示出显型由小脑的概括发作,过高热,或 optogenetic 刺激触发了的象 PKD 一样。有在小脑的小粒房间(GC ) 的特定的 PRRT2 删除的变异的老鼠概括在 Prrt2 空的老鼠看见的行为的显型。而且,记录在显示出的小脑的片做了在短暂举起的 GC 结果的刺激由 Purkinje 房间开火的抑制跟随了的那 optogenetic。在 PKD 处理使用的 anticonvulsant 药 carbamazepine 也在变异的老鼠减轻了象 PKD 一样行为。一起,我们的调查结果作为 SNARE 建筑群的一个新奇管理者识别 PRRT2 并且提供位于 PRRT2 相关的行为下面的电路机制。 | Guo-He Tan Yuan-Yuan Liu Lu Wang Kui Li Ze-Qiang Zhang Hong-Fu Li Zhong-Fei Yang Yang Li Dan Li Ming-Yue Wu Chun-Lei Yu Juan-Juan Long Ren-Chao Chen Li-Xi Li Lu-Ping Yin Ji-Wei Liu Xue-Wen Cheng Qi Shen You-Sheng Shu Kenji Sakimura Lu-Jian Liao Zhi-Ying Wu Zhi-Qi Xiong | 2018 | Cell Research2018，28，1： | 14 |
| 10 | Relationships between vegetation and stomata,and between vegetation and pollen surface soil in Yunnan,Southwest China显示文摘Surface pollen and stomata of 61 samples collected in a study area ranging from tropical seasonal rainforest to oak forest(Quercus spinosa) in the Yulong Snow Mountain region in Yunnan,China,are used to distinguish vegetation communities.The results show that tropical seasonal rainforest(and mountain rainforest),south subtropical evergreen broad-leaved forest,and Quercus shrub are distinguished effectively from other vegetation types by analysis of surface pollen.The south subtropical evergreen broad-leaved forest,Pinus kesiya forest and evergreen broadleaf forest are distinguished effectively from other types of vegetation by pollen analysis.However,P.yunnanensis forest is not distinguished from other vegetation types,and P.armandii,P.densata forest and temperate deciduous conifer mixed forest are not distinguished.The over-representation of Pinus pollen is the main reason that these vegetation communities are not distinguished from each other.Conifer stomata analysis is an effective tool for identifying and distinguishing different types of coniferous forest,and this method performs well even with a small number of sampling points. | SHEN HuaDong LI ChunHai WAN HeWen TONG GuoBang LIU JinSong DAN Johnson | 2013 | Chinese Science Bulletin2013，58，15： | 12 |
| 11 | Effects of operational factors on soluble microbial products in a carrier anaerobic baffled reactor treating dilute wastewater显示文摘The effects of feed strength, hydraulic residence time (HRT), and operational temperatures on soluble microbial product (SMP) production were investigated, to gain insights into the production mechanism. A carrier anaerobic baffled reactor (CABR) treating dilute wastewater was operated under a wide range of operational conditions, namely, feed strengths of 300–600 mg/L, HRTs of 9– 18 h, and temperatures of 10–28°C. Generally, SMP production increased with increasing feed strength and decreasing temperature. At high temperature (28°C), SMP production increased with decreasing HRT. As the temperature was decreased to 18 and 10°C, the SMP production was at its peak for 12 h HRT. Therefore, temperature could be an important determinant of SMP production along with HRT. A higher SMP to soluble chemical oxygen demand (SCOD) ratio was found at high temperature and long HRT because of complete volatile fatty acid degradation. SMP accounted for 50%–75% of the SCOD in the last chamber of the CABR. As a secondary metabolite, some SMP could be consumed at lower feed strength. | FENG Huajun HU Lifang SHAN Dan FANG Chengran HE Yonghua SHEN Dongsheng | 2008 | Journal of Environmental Sciences2008，20，6： | 12 |
| 12 | Effect of kidney-reinforcing,blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesenchymal stem cells from chronic aplastic anemia patients显示文摘OBJECTIVE:To explore the effect of kidney-reinforcing,blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesenchymal stem cells(MSCs) from patients with chronic aplastic anemia(CAA).METHODS:We used threeTraditional Chinese Medicine recipes,namely a kidney-reinforcing recipe(KRR),blood-activating and stasis-removing recipe(BASRR),and kidney-reinforcing,blood-activating and stasis-removing recipe(KRBASRR),and a normal saline control to prepare herbal medicine serum in Sprague Dawley rats.Thirty CAA patients were enrolled in the experimental group,including 17 kidney-Yang deficient patients and 13 kidney-Yin deficient patients.Ten healthy individuals were included in the control group.MSCs were isolated from bone marrow samples,and the cell density was observed to measure their proliferation ability by microscopy on days 2,7,and 14 after isolation.In addition,the expression of adhesion molecules of bone marrow MSCs(CD106,CD49d,CD31 and CD44) were detected by flow cytometry after 48 h of treatment with the four different herbal medicine serums.RESULTS:The proliferation of MSCs from kidney-Yang deficient and kidney-Yin deficient patients was weaker than that of MSCs from the control group.The expression of all adhesion molecules of bone marrow MSCs from CAA patients was obviously lower than that in the control group(P< 0.01).The expression of CD49d and CD31 in MSCs from patients with a kidney-Yin deficiency was lower than in those with a kidney-yang deficiency(P< 0.05 and P<0.01,respectively).For kidney-Yang deficient patients,CD31 expression in the KRBASRR group was significantly higher than that in the BASRR group(P<0.01),while CD44 in the KRBASRR group was significantly higher than that in both KRR and BASRR groups(P<0.01).For kidney-Yin deficient patients,CD106 and CD49d expression in the KRBASRR group was obviously higher than that in the KRR group(P<0.05),while CD31 and CD44 expression in the KRBASRR group was significantly higher than that in both KRR and BASRR groups(P< 0.05 and P<0.01,respectively).CONCLUSION:The bone marrow microenvironment in CAA patients is abnormal.The effect of KRBASRR may be better than that of KRR and BASRR for kidney-Yang deficient and kidney-Yin deficient patients by improving the expression levels of MSC adhesion molecules. | Baodong Ye Dan Chen Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qinhong Yu | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 12 |
| 13 | Identification of aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma by cDNA microarray and gene set enrichment analysis显示文摘 | Wenling Zhang Zhaoyang Zeng Yanhong Zhou Wei Xiong Songqing Fan Lan Xiao Donghai Huang Zheng Li Dan Li Minghua Wu Xiaoling Li Shourong Shen Rong Wang Li Cao Ke Tang Guiyuan Li | 2009 | Acta Biochimica et Biophysica Sinica2009，41，5： | 11 |
| 14 | Optimal antiarrhythmic drug therapy for electrical storm显示文摘Electrical storm,defined as 3 or more separate episodes of ventricular tachycardia or ventricular fibrillation within 24 hours,carries significant morbidity and mortality.These unstable ventricular arrhythmias have been described with a variety of conditions including ischemic heart disease,structural heart disease,and genetic conditions.While implantable cardioverter defibrillator implantation and ablation may be indicated and required,antiarrhythmic medication remains an important adjunctive therapy for these persons. | Dan Sorajja Thomas M.Munger Win-Kuang Shen | 2015 | The Journal of Biomedical Research2015，29，1： | 11 |
| 15 | Comparative analysis of the complete chloroplast genome among Prunus mume,P.armeniaca,and P.salicina显示文摘Prunus mume Sieb.et Zucc.,P.armeniaca L.,and P.salicina L.are economically important fruit trees in temperate regions.These species are taxonomically perplexing because of shared interspecific morphological traits and variation,which are mainly attributed to hybridization.The chloroplast is cytoplasmically inherited and often used for evolutionary studies.We sequenced the complete chloroplast genomes of P.mume,P.armeniaca,and P.salicina using Illumina sequencing followed by de novo assembly.The three chloroplast genomes exhibit a typical quadripartite structure with conserved genome arrangement,structure,and moderate divergence.The lengths of the genomes are 157,815,157,797,and 157,916 bp,respectively.The length of the large single-copy region(LSC)region is 86,113,86,283,and 86,122 bp,and the length of the SSC region is 18,916,18,734,and 19,028 bp;the IR region is 26,393,26,390,and 26,383 bp,respectively.Each of the three chloroplast genomes encodes 133 genes,including 94 protein-coding,31 tRNA,and eight rRNA genes.Differential gene analysis for the three species revealed that trnY-ATA is a unique gene in P.armeniaca;in contrast,the gene trnI-TAT is only present in P.mume and P.salicina,though the position of the gene in these chloroplast genomes differs.Further comparative analysis of the complete chloroplast genome sequences revealed that the ORF genes and the sequences of linked regions rps16 and atpA,atpH and atpI,trnc-GCA and psbD,ycf3 and atpB,and rpL32 and ndhD are significantly different and may be used as molecular markers in taxonomic studies.Phylogenetic evolution analysis of the three species suggests that P.mume has a closer genetic relationship to P.armeniaca than to P.salicina. | Song Xue Ting Shi Wenjie Luo Xiaopeng Ni Shahid Iqbal Zhaojun Ni Xiao Huang Dan Yao Zhijun Shen Zhihong Gao | 2019 | Horticulture Research2019，6，1： | 10 |
| 16 | Pretreatment neutrophil-to-lymphocyte and platelet-to- lymphocyte ratios do not predict survival in patients with cervical cancer treated with neoadjuvant chemotherapy and radical hysterectomy显示文摘 | WANG Dan WU Ming FENG Feng-zhi HUANG Hui-fang YANG Jia-xin SHEN Keng XIANG Yang | 2013 | Chinese Medical Journal2013，，8： | 10 |
| 17 | Development of the personalized criteria for microscopic review following four different series of hematology analyzer in a Chinese large scale hospital显示文摘 | CUI Wei WU Wei WANG Xin WANG Geng HAO Ying-ying CHEN Yu LUO Dan SHOU Wei-ling ZHANG Shuo XIANG Xue-fang SI Yong-zhen CHEN Qian CAI Hao LI Tan SHEN Han SHANG Kun ZHANG Yong-qiang | 2010 | Chinese Medical Journal2010，，22： | 10 |
| 18 | Effects of Forest Type and Urbanization on Carbon Storage of Urban Forests in Changchun, Northeast China显示文摘Rapid urbanization has led to dramatic changes in urban forest structures and functions, and consequently affects carbon(C) storage in cities. In this study, field surveys were combined with high resolution images to investigate the variability of C storage of urban forests in Changchun, Northeast China. The main objectives of this study were to quantify the C storage of urban forests in Changchun City, Northeast China and understand the effects of forest type and urbanization on C storage of urban forests. The results showed that the mean C density and the total C storage of urban forests in Changchun were 4.41 kg/m2 and 4.74 × 108 kg, respectively. There were significant differences in C density among urban forest types. Landscape and relaxation forest(LF) had the highest C density with 5.41 kg/m2, while production and management forest(PF) had the lowest C density with 1.46 kg/m2. These differences demonstrate that urban forest type is an important factor needed to be considered when the C storage is accurately estimated. Further findings revealed significant differences in different gradients of urbanization, and the mean C density decreased from the first ring(6.99 kg/m2) to the fourth ring(2.87 kg/m2). The total C storage increased from the first ring to the third ring. These results indicate that C storage by urban forests will be significantly changed during the process of urbanization. The results can provide insights for decision-makers and urban planners to better understand the effects of forest type and urbanization on C storage of urban forests in Changchun, and make better management plans for urban forests. | ZHANG Dan ZHENG Haifeng REN Zhibin ZHAI Chang SHEN Guoqiang MAO Zhixia WANG Peijiang HE Xingyuan | 2015 | Chinese Geographical Science2015，25，2： | 9 |
| 19 | Effects of sepiolite on stabilization remediation of heavy metal-contaminated soil and its ecological evaluation显示文摘在重金属的补习的稳定污染了土壤因为它的划算和快速的实现,一直在获得突起。在这研究,象微生物引起的社区那样的微生物引起的性质和酶活动,象土壤 pH 和金属部分那样的化学性质,和在菠菜(Spinacia oleracea ) 的重金属累积在用海泡石估计稳定补习有效性被考虑。结果证明土壤 pH 价值与升起的海泡石集中增加了。顺序的抽取结果显示海泡石的增加把 Cd 和 Pb 的可交换的部分的重要数量变换成剩余形式。海泡石的处理被遵守从土壤把 Cd 和 Pb translocation 归结为菠菜的根和射击。分别地, Cd 和 Pb 的集中为根展出了 12.6%51.0% 和 11.5%46.0% 减小并且分别地,为射击的 0.9%46.2% 和 43.0%65.8% 减小与控制相比组织。真菌和放射菌类计数,以及在过氧化氢酶活动的增加,显示了那土壤新陈代谢的恢复发生在海泡石处理以后。 | Yuebing SUN Dan ZHAO Yingming XU Lin WANG Xuefeng LIANG Yue SHEN | 2016 | Frontiers of Environmental Science & Engineering2016，10，1： | 9 |
| 20 | SURFACE ROUGHNESS PREDICTION MODEL FOR ULTRAPRECISION TURNING ALUMINIUM ALLOY WITH A SINGLE CRYSTAL DIAMOND TOOL显示文摘A surface roughness model utilizing regression analysis method is developed for predicting roughness of ultraprecision machined surface with a single crystal diamond tool. The effects of the main variables,such as cutting speed,feed,and depth of cut on surface roughness are also analyzed in diamond turning aluminum alloy. In order to predict and control the surface roughness before ultraprecision machining,constrained variable metric method is used to select the optimum cutting conditions during process planning. A lot of experimental results show that the model can predict the surface roughness effectively under a certain cutting conditions . | Wang Hongxiang Li Dan Dong shen Precision Engineering Research Institute, Harbin Institute of Technology, Harbin 150001, China | 2002 | Chinese Journal of Mechanical Engineering2002，15，2： | 8 |
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