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| 1 | Super-resolution fluorescence-assisted diffraction computational tomography reveals the threedimensional landscape of the cellular organelle interactome显示文摘The emergence of super-resolution(SR)fluorescence microscopy has rejuvenated the search for new cellular substructures.However,SR fluorescence microscopy achieves high contrast at the expense of a holistic view of the interacting partners and surrounding environment.Thus,we developed SR fluorescence-assisted diffraction computational tomography(SR-FACT),which combines label-free three-dimensional optical diffraction tomography(ODT)with two-dimensional fluorescence Hessian structured illumination microscopy.The ODT module is capable of resolving the mitochondria,lipid droplets,the nuclear membrane,chromosomes,the tubular endoplasmic reticulum,and lysosomes.Using dual-mode correlated live-cell imaging for a prolonged period of time,we observed novel subcellular structures named dark-vacuole bodies,the majority of which originate from densely populated perinuclear regions,and intensively interact with organelles such as the mitochondria and the nuclear membrane before ultimately collapsing into the plasma membrane.This work demonstrates the unique capabilities of SR-FACT,which suggests its wide applicability in cell biology in general. | Dashan Dong Xiaoshuai Huang Liuju Li Heng Mao Yanquan Mo Guangyi Zhang Zhe Zhang Jiayu Shen Wei Liu Zeming Wu Guanghui Liu Yanmei Liu Hong Yang Qihuang Gong Kebin Shi Liangyi Chen | 2020 | Light(Science & Applications)2020，9，1： | 7 |
| 2 | Robust and high-speed rotation control in optical tweezers by using polarization synthesis based on heterodyne interference显示文摘The rotation control of particles in optical tweezers is often subject to the spin or orbit angular momentum induced optical torque,which is susceptible to the mechanical and morphological properties of individual particle.Here we report on a robust and high-speed rotation control in optical tweezers by using a novel linear polarization synthesis based on optical heterodyne interference between two circularly polarized lights with opposite handedness.The synthesized linear polarization can be rotated in a hopping-free scheme at arbitrary speed determined electronically by the heterodyne frequency between two laser fields.The experimental demonstration of a trapped vaterite particle in water shows that the precisely controlled rotation frequency of 300 Hz can be achieved.The proposed method will find promising applications in optically driven micro-gears,fluidic pumps and rotational micro-rheology. | Wei Liu Dashan Dong Hong Yang Qihuang Gong Kebin Shi | 2020 | Opto-Electronic Advances2020，3，8： | 5 |
| 3 | Solving the missing cone problem by deep learning显示文摘In order to extract the quantitative three-dimensional(3-D)distribution of refractive index(RI)in live cells noninvasively,optical diffraction tomography(ODT)uses the non-ionizing light sources instead of x-rays to perform a computational holographic tomography. | Dashan Dong Kebin Shi | 2020 | Advanced Photonics2020，2，2： | 4 |
| 4 | Detour-phased perovskite ultrathin planar lens using direct femtosecond laser writing显示文摘Perovskite-enabled optical devices have drawn intensive interest and have been considered promising candidates for integrated optoelectronic systems.As one of the important photonic functions,optical phase modulation previously was demonstrated with perovskite substrate and complex refractive index engineering with laser scribing.Here we report on the new scheme of achieving efficient phase modulation by combining detour phase design with 40 nm ultrathin perovskite films composed of nanosized crystalline particles.Phase modulation was realized by binary amplitude patterning,which significantly simplifies the fabrication process.Perovskite nanocrystal films exhibit significantly weak ion migration effects under femtosecond laser writing,resulting in smooth edges along the laser ablated area and high diffractive optical quality.Fabrication of a detour-phased perovskite ultrathin planar lens with a diameter of 150μm using femtosecond laser scribing was experimentally demonstrated.A high-performance 3D focus was observed,and the fabrication showed a high tolerance with different laser writing powers.Furthermore,the high-quality imaging capability of perovskite ultrathin planar lenses with a suppressed background was also demonstrated. | WENKAI YANG LIGE LIU DASHAN DONG XIN ZHANG HAN LIN YUNKUN WANG HONG YANG YUNAN GAO HAIZHENG ZHONG BAOHUA JIA KEBIN SHI | 2022 | Photonics Research2022，10，12： | 0 |
| 5 | Reflective ultrathin light-sheet microscopy with isotropic 3D resolutions显示文摘Light-sheet fluorescence microscopy(LSFM)has played an important role in bio-imaging due to its advantages of high photon efficiency,fast speed,and long-term imaging capabilities.The perpendicular layout between LSFM excitation and detection often limits the 3D resolutions as well as their isotropy.Here,we report on a reflective type light-sheet microscope with a mini-prism used as an optical path reflector.The conventional high NA objectives can be used both in excitation and detection with this design.Isotropic resolutions in 3D down to300 nm could be achieved without deconvolution.The proposed method also enables easy transform of a conventional fluorescence microscope to high performance light-sheet microscopy. | YUE WANG DASHAN DONG WENKAI YANG RENXI HE MING LEI KEBIN SHI | 2024 | Photonics Research2024，12，2： | 0 |
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